Proprotein convertase subtilisin/kexin type 6 precursor

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Q63415 (PCSK6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proprotein convertase subtilisin/kexin type 6
EC=3.4.21.-

Alternative name(s):
Paired basic amino acid cleaving enzyme 4
Subtilisin-like proprotein convertase 4
Short name=SPC4
Subtilisin/kexin-like protease PACE4

Gene names

Name:	Pcsk6
Synonyms:	Pace4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Catalytic activity	Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Yaa-Arg-\|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.
Cofactor	Calcium Potential.
Tissue specificity	High expression in the anterior pituitary and in several brain regions, the atrium, and the ventricle.
Domain	The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum.
Sequence similarities	Belongs to the peptidase S8 family. Contains 1 homo B/P domain. Contains 1 PLAC domain.

Ontologies

Keywords
Domain	Repeat Signal
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptide hormone processing Inferred from expression pattern Ref.1. Source: RGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	serine-type endopeptidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 45

Potential

Propeptide

46 – 132

Potential

PRO_0000027112

Chain

133 – 937

805

Proprotein convertase subtilisin/kexin type 6

PRO_0000027113

Regions

Domain

477 – 615

139

Homo B/P

Domain

899 – 937

PLAC

Region

133 – 454

322

Catalytic

Region

680 – 898

219

CRM (Cys-rich motif)

Motif

534 – 536

Cell attachment site Potential

Sites

Active site

186

Charge relay system By similarity

Active site

227

Charge relay system By similarity

Active site

401

Charge relay system By similarity

Site

132 – 133

Cleavage; by autolysis

Amino acid modifications

Glycosylation

240

N-linked (GlcNAc...) Potential

Glycosylation

882

N-linked (GlcNAc...) Potential

Glycosylation

900

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q63415 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F3865557C33705C8
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FASTA

937

104,053

        10         20         30         40         50         60 
MPPRAPPAPG PRPPPRAAGR HGLSPLAPRP WRWLLLLALP AVCSALPPPR PVYTNHWAVQ 

        70         80         90        100        110        120 
VLGGPGAADR VAAAHGYLNL GQIGNLDDYY HFYHSKTFKR STLSSRGPHT FLRMDPQVKW 

       130        140        150        160        170        180 
LQQQEVKRRV KRQARSDSLY FNDPIWSNMW YMHCADKNSR CRSEMNVQAA WKRGYTGKNV 

       190        200        210        220        230        240 
VVTILDDGIE RNHPDLAPNY DSYASYDVNG NDYDPSPRYD ASNENKHGTR CAGEVAASAN 

       250        260        270        280        290        300 
NSYCIVGIAY NAKIGGIRML DGDVTDVVEA KSLGIRPNYI DIYSASWGPD DDGKTVDGPG 

       310        320        330        340        350        360 
RLAKQAFEYG IKKGRQGLGS IFVWASGNGG REGDHCSCDG YTNSIYTISV SSTTENGHKP 

       370        380        390        400        410        420 
WYLEECASTL ATTYSSGAFY ERKIVTTDLR QRCTDGHTGT SVSAPMVAGI IALALEANNQ 

       430        440        450        460        470        480 
LTWRDVQHLL VKTSRPAHLK ASDWKVNGAG HKVSHLYGFG LVDAEALVLE ARKWTAVPSQ 

       490        500        510        520        530        540 
HMCVATADKR PRSIPVVQVL RTTALTNACA DHSDQRVVYL EHVVVRISIS HPRRGDLQIH 

       550        560        570        580        590        600 
LISPSGTKSQ LLAKRLLDFS NEGFTNWEFM TVHCWGEKAE GEWTLEVQDI PSQVRNPEKQ 

       610        620        630        640        650        660 
GKLKEWSLIL YGTAEHPYRT FSSHQSRSRM LELSVPEQEP LKAEGPPPQA ETPEEEEEYT 

       670        680        690        700        710        720 
GVCHPECGDK GCDGPSADQC LNCVHFSLGN SKTNRKCVSE CPLGYFGDTA ARRCRRCHKG 

       730        740        750        760        770        780 
CETCTGRSPT QCLSCRRGFY HHQETNTCVT LCPAGLYADE SQRLCLRCHP SCQKCVDEPE 

       790        800        810        820        830        840 
KSTVCKEGFS LARGSCIPDC EPGTYFDSEL IRCGECHHTC RTCVGPSREE CIHCAKSFHF 

       850        860        870        880        890        900 
QDWKCVPACG EGFYPEEMPG LPHKVCRRCD ENCLSCEGSS RNCSRCKAGF TQLGTSCITN 

       910        920        930 
HTCSNADETF CEMVKSNRLC ERKLFIQFCC RTCLLAG

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References

[1]

"PACE4: a subtilisin-like endoprotease prevalent in the anterior pituitary and regulated by thyroid status."
Johnson R.C., Darlington D.N., Hand T.A., Bloomquist B.T., Mains R.E.
Endocrinology 135:1178-1185(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Hypothalamus and Pituitary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31894 mRNA. Translation: AAA61987.1.
IPI	IPI00208966.
PIR	I53282.
RefSeq	NP_037131.1. NM_012999.1.
UniGene	Rn.950.
3D structure databases
ProteinModelPortal	Q63415.
SMR	Q63415. Positions 142-616.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000060741.
Protein family/group databases
MEROPS	S08.075.
Proteomic databases
PaxDb	Q63415.
PRIDE	Q63415.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25507.
KEGG	rno:25507.
UCSC	RGD:3246. rat.
Organism-specific databases
CTD	5046.
RGD	3246. Pcsk6.
Phylogenomic databases
eggNOG	COG1404.
HOGENOM	HOG000192536.
HOVERGEN	HBG008705.
KO	K08672.
Gene expression databases
ArrayExpress	Q63415.
Genevestigator	Q63415.
GermOnline	ENSRNOG00000011526. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.200. 1 hit.
InterPro	IPR000742. EG-like_dom. IPR006212. Furin_repeat. IPR008979. Galactose-bd-like. IPR009030. Growth_fac_rcpt. IPR000209. Peptidase_S8/S53_dom. IPR023827. Peptidase_S8_Asp-AS. IPR022398. Peptidase_S8_His-AS. IPR023828. Peptidase_S8_Ser-AS. IPR015500. Peptidase_S8_subtilisin-rel. IPR010909. PLAC. IPR009020. Prot_inh_propept. IPR002884. PrprotnconvertsP. [Graphical view]
PANTHER	PTHR10795. PTHR10795. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. PF08686. PLAC. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
SMART	SM00181. EGF. 1 hit. SM00261. FU. 5 hits. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF57184. Grow_fac_recept. 2 hits. SSF52743. Pept_S8_S53. 1 hit. SSF54897. Prot_inh_propept. 1 hit.
PROSITE	PS50900. PLAC. 1 hit. PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q63415.
ChEMBL	CHEMBL5507.
NextBio	606929.

Entry information

Entry name

PCSK6_RAT

Accession

Primary (citable) accession number: Q63415

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents