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Protein

Proprotein convertase subtilisin/kexin type 6

Gene

Pcsk6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.

Catalytic activityi

Release of mature proteins from their proproteins by cleavage of Arg-Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys.

Cofactori

Ca2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186Charge relay systemBy similarity1
Active sitei227Charge relay systemBy similarity1
Active sitei401Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • peptide hormone processing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BRENDAi3.4.21.B25. 5301.

Protein family/group databases

MEROPSiS08.075.

Names & Taxonomyi

Protein namesi
Recommended name:
Proprotein convertase subtilisin/kexin type 6 (EC:3.4.21.-)
Alternative name(s):
Paired basic amino acid cleaving enzyme 4
Subtilisin-like proprotein convertase 4
Short name:
SPC4
Subtilisin/kexin-like protease PACE4
Gene namesi
Name:Pcsk6
Synonyms:Pace4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3246. Pcsk6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 451 PublicationAdd BLAST45
PropeptideiPRO_000002711246 – 1321 PublicationAdd BLAST87
ChainiPRO_0000027113133 – 937Proprotein convertase subtilisin/kexin type 6Add BLAST805

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi882N-linked (GlcNAc...)Sequence analysis1
Glycosylationi900N-linked (GlcNAc...)Sequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei132 – 133Cleavage; by autolysis2

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ63415.
PRIDEiQ63415.

Expressioni

Tissue specificityi

High expression in the anterior pituitary and in several brain regions, the atrium, and the ventricle.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015845.

Chemistry databases

BindingDBiQ63415.

Structurei

3D structure databases

ProteinModelPortaliQ63415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini181 – 468Peptidase S8Add BLAST288
Repeati660 – 707FU 1Add BLAST48
Repeati711 – 758FU 2Add BLAST48
Repeati762 – 806FU 3Add BLAST45
Repeati810 – 855FU 4Add BLAST46
Repeati863 – 911FU 5Add BLAST49
Domaini899 – 937PLACPROSITE-ProRule annotationAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni680 – 898CRM (Cys-rich motif)Add BLAST219

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi534 – 536Cell attachment siteSequence analysis3

Domaini

The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum.

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 5 FU (furin-like) repeats.Curated
Contains 1 peptidase S8 domain.Curated
Contains 1 PLAC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ63415.
KOiK08672.
PhylomeDBiQ63415.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000742. EGF-like_dom.
IPR006212. Furin_repeat.
IPR008979. Galactose-bd-like.
IPR032778. GF_recep_IV.
IPR009030. Growth_fac_rcpt_.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010909. PLAC.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF14843. GF_recep_IV. 1 hit.
PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF08686. PLAC. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SMARTiSM00181. EGF. 5 hits.
SM00261. FU. 5 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS50900. PLAC. 1 hit.
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63415-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRAPPAPG PRPPPRAAGR HGLSPLAPRP WRWLLLLALP AVCSALPPPR
60 70 80 90 100
PVYTNHWAVQ VLGGPGAADR VAAAHGYLNL GQIGNLDDYY HFYHSKTFKR
110 120 130 140 150
STLSSRGPHT FLRMDPQVKW LQQQEVKRRV KRQARSDSLY FNDPIWSNMW
160 170 180 190 200
YMHCADKNSR CRSEMNVQAA WKRGYTGKNV VVTILDDGIE RNHPDLAPNY
210 220 230 240 250
DSYASYDVNG NDYDPSPRYD ASNENKHGTR CAGEVAASAN NSYCIVGIAY
260 270 280 290 300
NAKIGGIRML DGDVTDVVEA KSLGIRPNYI DIYSASWGPD DDGKTVDGPG
310 320 330 340 350
RLAKQAFEYG IKKGRQGLGS IFVWASGNGG REGDHCSCDG YTNSIYTISV
360 370 380 390 400
SSTTENGHKP WYLEECASTL ATTYSSGAFY ERKIVTTDLR QRCTDGHTGT
410 420 430 440 450
SVSAPMVAGI IALALEANNQ LTWRDVQHLL VKTSRPAHLK ASDWKVNGAG
460 470 480 490 500
HKVSHLYGFG LVDAEALVLE ARKWTAVPSQ HMCVATADKR PRSIPVVQVL
510 520 530 540 550
RTTALTNACA DHSDQRVVYL EHVVVRISIS HPRRGDLQIH LISPSGTKSQ
560 570 580 590 600
LLAKRLLDFS NEGFTNWEFM TVHCWGEKAE GEWTLEVQDI PSQVRNPEKQ
610 620 630 640 650
GKLKEWSLIL YGTAEHPYRT FSSHQSRSRM LELSVPEQEP LKAEGPPPQA
660 670 680 690 700
ETPEEEEEYT GVCHPECGDK GCDGPSADQC LNCVHFSLGN SKTNRKCVSE
710 720 730 740 750
CPLGYFGDTA ARRCRRCHKG CETCTGRSPT QCLSCRRGFY HHQETNTCVT
760 770 780 790 800
LCPAGLYADE SQRLCLRCHP SCQKCVDEPE KSTVCKEGFS LARGSCIPDC
810 820 830 840 850
EPGTYFDSEL IRCGECHHTC RTCVGPSREE CIHCAKSFHF QDWKCVPACG
860 870 880 890 900
EGFYPEEMPG LPHKVCRRCD ENCLSCEGSS RNCSRCKAGF TQLGTSCITN
910 920 930
HTCSNADETF CEMVKSNRLC ERKLFIQFCC RTCLLAG
Length:937
Mass (Da):104,053
Last modified:November 1, 1996 - v1
Checksum:iF3865557C33705C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31894 mRNA. Translation: AAA61987.1.
PIRiI53282.
RefSeqiNP_037131.1. NM_012999.1.
UniGeneiRn.950.

Genome annotation databases

GeneIDi25507.
KEGGirno:25507.
UCSCiRGD:3246. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31894 mRNA. Translation: AAA61987.1.
PIRiI53282.
RefSeqiNP_037131.1. NM_012999.1.
UniGeneiRn.950.

3D structure databases

ProteinModelPortaliQ63415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015845.

Chemistry databases

BindingDBiQ63415.
ChEMBLiCHEMBL5507.

Protein family/group databases

MEROPSiS08.075.

Proteomic databases

PaxDbiQ63415.
PRIDEiQ63415.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25507.
KEGGirno:25507.
UCSCiRGD:3246. rat.

Organism-specific databases

CTDi5046.
RGDi3246. Pcsk6.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ63415.
KOiK08672.
PhylomeDBiQ63415.

Enzyme and pathway databases

BRENDAi3.4.21.B25. 5301.

Miscellaneous databases

PROiQ63415.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000742. EGF-like_dom.
IPR006212. Furin_repeat.
IPR008979. Galactose-bd-like.
IPR032778. GF_recep_IV.
IPR009030. Growth_fac_rcpt_.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010909. PLAC.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 2 hits.
PfamiPF14843. GF_recep_IV. 1 hit.
PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF08686. PLAC. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SMARTiSM00181. EGF. 5 hits.
SM00261. FU. 5 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS50900. PLAC. 1 hit.
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCSK6_RAT
AccessioniPrimary (citable) accession number: Q63415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.