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Protein

Guanine nucleotide exchange factor DBS

Gene

Mcf2l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways. Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-193648 NRAGE signals death through JNK
R-RNO-194840 Rho GTPase cycle
R-RNO-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor DBS
Alternative name(s):
DBL's big sister
MCF2-transforming sequence-like protein
OST oncogene1 Publication
Gene namesi
Name:Mcf2l
Synonyms:Ost1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi619782 Mcf2l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809371 – 1149Guanine nucleotide exchange factor DBSAdd BLAST1149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei457PhosphoserineCombined sources1
Modified residuei462PhosphoserineCombined sources1
Modified residuei471PhosphoserineCombined sources1
Modified residuei480PhosphoserineCombined sources1
Modified residuei621PhosphoserineBy similarity1
Modified residuei622PhosphothreonineBy similarity1
Modified residuei1033PhosphoserineBy similarity1
Modified residuei1034PhosphoserineBy similarity1
Modified residuei1041PhosphoserineBy similarity1
Modified residuei1042PhosphoserineBy similarity1

Post-translational modificationi

Mainly phosphorylated on serine.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63406
PRIDEiQ63406

PTM databases

iPTMnetiQ63406
PhosphoSitePlusiQ63406

Expressioni

Tissue specificityi

Highest expression in the brain, where it is found in neurons and alpha-tanycytes (at protein level). Detected in brain, and at lower levels in the heart.1 Publication

Gene expression databases

BgeeiENSRNOG00000028426
ExpressionAtlasiQ63406 baseline and differential
GenevisibleiQ63406 RN

Interactioni

Subunit structurei

Interacts with GTP-bound RAC1 (PubMed:7957046). Interacts with CDC42. Interacts with RHOA. Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • Rho guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

CORUMiQ63406
STRINGi10116.ENSRNOP00000023352

Structurei

3D structure databases

SMRiQ63406
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 224CRAL-TRIOPROSITE-ProRule annotationAdd BLAST173
Repeati355 – 454SpectrinAdd BLAST100
Domaini632 – 812DHPROSITE-ProRule annotationAdd BLAST181
Domaini830 – 946PHPROSITE-ProRule annotationAdd BLAST117
Domaini1055 – 1116SH3PROSITE-ProRule annotationAdd BLAST62

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili503 – 528Sequence analysisAdd BLAST26

Domaini

The CRAL-TRIO domain mediates interaction with various inositol phospholipids, such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).By similarity
The DH domain is involved in interaction with CCPG1.By similarity

Sequence similaritiesi

Belongs to the MCF2 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410IPNH Eukaryota
ENOG410XNYV LUCA
GeneTreeiENSGT00760000119030
HOGENOMiHOG000231361
HOVERGENiHBG062385
InParanoidiQ63406

Family and domain databases

CDDicd01227 PH_Dbs, 1 hit
cd00160 RhoGEF, 1 hit
cd00170 SEC14, 1 hit
cd11857 SH3_DBS, 1 hit
Gene3Di1.20.900.101 hit
2.30.29.301 hit
InterProiView protein in InterPro
IPR001251 CRAL-TRIO_dom
IPR036865 CRAL-TRIO_dom_sf
IPR035899 DBL_dom_sf
IPR035534 DBS_PH
IPR035532 DBS_SH3
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF13716 CRAL_TRIO_2, 1 hit
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00435 Spectrin, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00516 SEC14, 1 hit
SM00326 SH3, 1 hit
SM00150 SPEC, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
SSF52087 SSF52087, 1 hit
PROSITEiView protein in PROSITE
PS50191 CRAL_TRIO, 1 hit
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q63406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNCWCFIFC KERVRSNSSS PQHDGTSREE ADHQVDVSDG IRLVPDKAEA
60 70 80 90 100
TMATASDEIM HQDIVPLCAA DIQEQLKKRF AYLSGGRGQD GSPVITFPDY
110 120 130 140 150
PAFSEIPDKE FQNVMTYLTS IPSLQDAGIG FILVIDRRQD KWTSVKASVL
160 170 180 190 200
RIAASFPANL QLVLVLRPTG FFQRTLSDIA FKFNRDEFKM KVPVMMLSSV
210 220 230 240 250
PELHGYIDKS QLTEDLGGTL DYCHSRWLCH RTAIESFALM VKQTAQMLQA
260 270 280 290 300
FGTELAETEL PNDVQSTSLV LSAHTEKKAK VKEDLQLALT EGNSILESLR
310 320 330 340 350
EPLAESIVHS VNQDQLDNQA TVKRLLTQLN ETEAAFDEFW AKHQQKLEQC
360 370 380 390 400
LQLRHFEQGF REVKTALDSM SQKIAAFTDV GNSLAHVQHL LKDLTTFEEK
410 420 430 440 450
SSVAVDKARA LSLEGQQLIE NRHYAVDSIH PKCEELQHLC DHFASEVTRR
460 470 480 490 500
RDLLSKSLEL HSLLETSMKW SDEGIFLLAS QPVDKCQSQD GAEAALQEIE
510 520 530 540 550
KFLETGAENK IQELNKIYKE YECILNQDLL EHVQKVFQKQ ESTEEMFHRR
560 570 580 590 600
QASLKKLAAK QTRPVQPVAP RPEALTKSPS PSPGSWRSSE NSSSEGNALR
610 620 630 640 650
RGPYRRAKSE MSEPRQGRTS STGEEEESLA ILRRHVMNEL LDTERAYVEE
660 670 680 690 700
LLCVLEGYAA EMDNPLMAHL ISTGLQNKKN ILFGNMEEIY HFHNRIFLRE
710 720 730 740 750
LESCIDCPEL VGRCFLERME EFQIYEKYCQ NKPRSESLWR QCSDCPFFQE
760 770 780 790 800
CQKKLDHKLS LDSYLLKPVQ RITKYQLLLK EMLKYSKHCE GAEDLQEALS
810 820 830 840 850
SILGILKAVN DSMHLIAITG YDGNLGDLGK LLMQGSFSVW TDHKKGHTKV
860 870 880 890 900
KELARFKPMQ RHLFLHEKAV LFCKKREENG EGYEKAPSYS YKQSLNMTAV
910 920 930 940 950
GITENVKGDT KKFEIWYNAR EEVYIIQAPT PEIKAAWVNE IRKVLTSQLQ
960 970 980 990 1000
ACREASQHRA LEQSHSLPLP TPASTSPTKG STRNVKKLED RKTDPLCLEG
1010 1020 1030 1040 1050
CVSSSLPKPP EKGKGWSKTS HSLEAPEEDG GWSSAEELIN SSDAEEDGGV
1060 1070 1080 1090 1100
GPRKLVPGKY TVLMDGEKGG SDTLAMRSGD MVEVVEEGTE GLWYVRDLTS
1110 1120 1130 1140
SKEGWVPASS LATLLGKSSS AQCLSSSGKT HCARQLCPEP AKILSPEPV
Length:1,149
Mass (Da):129,410
Last modified:April 20, 2010 - v3
Checksum:i1735D8565A965963
GO

Sequence cautioni

The sequence CAA84713 differs from that shown. Probable cloning artifact.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC117058 Genomic DNA No translation available.
Z35654 mRNA Translation: CAA84713.1 Sequence problems.
PIRiS51620
UniGeneiRn.81062

Genome annotation databases

EnsembliENSRNOT00000043170; ENSRNOP00000047024; ENSRNOG00000028426
UCSCiRGD:619782 rat

Similar proteinsi

Entry informationi

Entry nameiMCF2L_RAT
AccessioniPrimary (citable) accession number: Q63406
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 20, 2010
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome