Q633V5 (NTPA_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BCE33L4233

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178122
Regions
	Region	11 – 16	6	Substrate binding By similarity
	Region	73 – 74	2	Substrate binding By similarity
Sites
	Metal binding	44	1	Magnesium or manganese By similarity
	Metal binding	73	1	Magnesium or manganese By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q633V5 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 09C1E0693E774682	FASTA	205	23,223
	10 20 30 40 50 60 MENMKQVVVA TKNMGKVREF AELFERFDLE VKSLHDFPHI EEVEETGETF EENAILKADS 70 80 90 100 110 120 LSRQLNAIVI ADDSGLIVDA LNGKPGVYSA RFAGEPKDDQ ANIDKVLQEL NEVAFEKRKA 130 140 150 160 170 180 RFYCALAVAF PEGDKKPVIV NGTCEGFILE QRRGENGFGY DPIFYVEEYK KAMAELSSDE 190 200 KNAISHRGRA LRKLEEKIPE WFLGE

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU16035.1.
RefSeq	YP_085812.1. NC_006274.1.
3D structure databases
ProteinModelPortal	Q633V5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q633V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000042647; EBBACP00000041548; EBBACG00000042638.
GeneID	3024790.
GenomeReviews	Gene locus BCE33L4233 in contig CP000001_GR.
KEGG	bcz:BCZK4233.
PATRIC	18892303. VBIBacCer95304_4486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
GeneTree	EBGT00050000001334.
HOGENOM	HBG697237.
OMA	GEAHNDA.
PhylomeDB	Q633V5.
ProtClustDB	PRK14822.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L4233-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BACCZ

Accession

Primary (citable) accession number: Q633V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program