Reviewed, UniProtKB/Swiss-Prot Q633V4 (RNPH_BACCZ)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BCE33L4234

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease PH HAMAP MF_00564		PRO_0000139865

Sequences

	Sequence		Length	Mass (Da)
	Q633V4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 00053DA2C582D0E6	FASTA	245	26,983
	10 20 30 40 50 60 MRVDGREKTE LRHIHIHTNY LKHPEGSVLI EVGDTKVICS ATIEERVPPF MRGEGKGWVT 70 80 90 100 110 120 AEYAMIPRAT EQRTIRESSK GKVTGRTMEI QRLIGRALRA VVDLEALGER TVWIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAYVAMVLA FEKLLQAEKV SKIPVKDYLA ATSVGIVEEQ GVVLDLNYAE 190 200 210 220 230 240 DSKADVDMNV IMTGKGQFVE VQGTGEEATF SRAQLNELLD AAEQGIFQLI DIQKEALGDI VSHIE

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000001 Genomic DNA. Translation: AAU16036.1.
RefSeq	YP_085813.1.
3D structure databases
SMR	Q633V4. Positions 1-239.
ModBase	Search...
Protein-protein interaction databases
STRING	Q633V4.
Genome annotation databases
GeneID	3022779.
GenomeReviews	Gene locus BCE33L4234 in contig CP000001_GR.
KEGG	bcz:BCZK4234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q633V4.
OMA	YAMLPRA.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L4234-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BACCZ

Accession

Primary (citable) accession number: Q633V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)