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Q63358

- MYO9B_RAT

UniProt

Q63358 - MYO9B_RAT

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Protein

Unconventional myosin-IXb

Gene

Myo9b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2468ATPSequence Analysis
Zinc fingeri1592 – 164150Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. actin binding Source: RGD
  2. actin-dependent ATPase activity Source: RGD
  3. actin filament binding Source: RGD
  4. ADP binding Source: RGD
  5. ATPase activity Source: RGD
  6. ATP binding Source: RGD
  7. ATP-dependent protein binding Source: RGD
  8. GTPase activator activity Source: RGD
  9. microfilament motor activity Source: Ensembl
  10. Rho GTPase activator activity Source: RGD
  11. zinc ion binding Source: RGD

GO - Biological processi

  1. ATP catabolic process Source: RGD
  2. establishment of cell polarity Source: Ensembl
  3. lamellipodium morphogenesis Source: Ensembl
  4. macrophage chemotaxis Source: Ensembl
  5. monocyte chemotaxis Source: Ensembl
  6. Rho protein signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-IXb
Alternative name(s):
Unconventional myosin-9b
Gene namesi
Name:Myo9b
Synonyms:Myr5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3146. Myo9b.

Subcellular locationi

Cytoplasmcell cortex By similarity. Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeleton By similarity
Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.By similarity

GO - Cellular componenti

  1. actin filament Source: RGD
  2. cell cortex Source: Ensembl
  3. cytoplasm Source: RGD
  4. filamentous actin Source: Ensembl
  5. filopodium tip Source: Ensembl
  6. lamellipodium Source: RGD
  7. myosin complex Source: UniProtKB-KW
  8. perinuclear region of cytoplasm Source: RGD
  9. ruffle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 19801979Unconventional myosin-IXbPRO_0000123471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei717 – 7171PhosphoserineBy similarity
Modified residuei1177 – 11771PhosphoserineBy similarity
Modified residuei1220 – 12201PhosphoserineBy similarity
Modified residuei1243 – 12431PhosphoserineBy similarity
Modified residuei1266 – 12661PhosphoserineBy similarity
Modified residuei1319 – 13191PhosphothreonineBy similarity
Modified residuei1327 – 13271PhosphoserineBy similarity
Modified residuei1952 – 19521PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63358.
PRIDEiQ63358.

PTM databases

PhosphoSiteiQ63358.

Expressioni

Tissue specificityi

Expressed in testis, lung, thymus, brain, liver, spleen and heart muscle. Detected in lung, testis, spleen and liver, and at reduced level in different brain regions (at protein level).1 Publication

Developmental stagei

During forebrein development, it is expressed at higher levels in embryonic and early postnatal stages than in the adult forebrain.1 Publication

Gene expression databases

ExpressionAtlasiQ63358. baseline.
GenevestigatoriQ63358.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Arfgef1D4A6314EBI-6251137,EBI-6251168

Protein-protein interaction databases

IntActiQ63358. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ63358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 114100Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini146 – 954809Myosin motorAdd
BLAST
Domaini958 – 97821IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini981 – 100121IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini1002 – 102423IQ 3PROSITE-ProRule annotationAdd
BLAST
Domaini1025 – 105430IQ 4PROSITE-ProRule annotationAdd
BLAST
Domaini1663 – 1848186Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni845 – 85612Actin-bindingAdd
BLAST
Regioni941 – 1045105Neck or regulatory domainAdd
BLAST
Regioni1046 – 1980935TailAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1841 – 186121Sequence AnalysisAdd
BLAST
Coiled coili1918 – 194831Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 4 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1592 – 164150Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5022.
HOGENOMiHOG000113707.
HOVERGENiHBG052558.
InParanoidiQ63358.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13140:SF306. PTHR13140:SF306. 1 hit.
PfamiPF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63358-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAHEAGSSG RRRPATFHLH IYPQLPSAGS QTSCRVTATK DSTTSDVIRD
60 70 80 90 100
VVASLHLDGS KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQKEHP
110 120 130 140 150
REDGYYFLLQ ERNADGSIQY LHVQLLAQPT AACRLVERGL LPRPQADFDD
160 170 180 190 200
LCNLPELNEA NLLQSLKLRF VQQKIYTYAG SILVAINPFK FLPIYNPKYV
210 220 230 240 250
KMYENQQLGK LEPHVFALAD VAYYTMLRKH VNQCIVISGE SGSGKTQSTN
260 270 280 290 300
FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI
310 320 330 340 350
QVNYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER
360 370 380 390 400
QEFQLKQPQD YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ
410 420 430 440 450
IFSVLSAILY LGNVTYKKRA TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV
460 470 480 490 500
EVLTKRKTIT VNDKLILPYS LSEAITARDS MAKSLYSALF DWIVLRINHA
510 520 530 540 550
LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA NEQLQYYFTQ
560 570 580 590 600
HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP
610 620 630 640 650
HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK
660 670 680 690 700
NMDYMRPDIV ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE
710 720 730 740 750
AGRLRAERAE KAEAGVSSPV TRSHVEELPR GANTPSEKLY RDLHNQIIKS
760 770 780 790 800
LKGLPWQGED PRRLLQSLSR LQKPRTFFLK SKGIKQKQII PKNLLDSKSL
810 820 830 840 850
RLIISMTLHD RTTKSLLHLH KKKKPPSISA QFQTSLNKLL EALGKAEPFF
860 870 880 890 900
IRCIRSNAEK KELCFDDELV LQQLRYTGML ETVRIRRSGY SAKYTFQDFT
910 920 930 940 950
EQFQVLLPKD VQPCREAIAA LLEKLQVDRQ NYQIGKTKVF LKETERQALQ
960 970 980 990 1000
ERLHGEVLRR ILLLQSWFRM VLERRHFVQM KHAALTIQAC WRSYRVRRTL
1010 1020 1030 1040 1050
ERTRAAVYLQ AAWRGYLQRQ AYHHQRHSII RLQSLCRGHL QRRSFSQMML
1060 1070 1080 1090 1100
EKQKAEQARE TAGAEMSEGE PSPVAAGEQP SEHPVEDPES LGVETETWMN
1110 1120 1130 1140 1150
SKSPNGLSPK KEIPSPEMET PAQKTVPAES HEKVPSSREK RESRRQRGLE
1160 1170 1180 1190 1200
HVERQNKHIQ SCREENSTLR EPSRKASLET GESFPEDTKE PREDGLETWT
1210 1220 1230 1240 1250
ETAAPSCPKQ VPIVGDPPRS PSPLQRPASL DLDSRVSPVL PSSSLESPQD
1260 1270 1280 1290 1300
EDKGENSTKV QDKPESPSGS TQIQRYQHPD TERLATAVEI WRGKKLASAM
1310 1320 1330 1340 1350
LSQSLDLSEK PRTAGAALTP TEERRISFST SDVSKLSPVK TSTEVDGDLS
1360 1370 1380 1390 1400
AKKPAGHKKK SEDPSAGPDA GLPTGSQGDS KSAFKRLFLH KAKDKKPSLE
1410 1420 1430 1440 1450
GVEETEGSGG QAAQEAPARK TLDVPSSQQH RHTTGEKPLK GKKNRNRKVG
1460 1470 1480 1490 1500
QITVSEKWRE SVFRKITNAN ELKFLDEFLL NKVNDLRSQK TPIESLFIEA
1510 1520 1530 1540 1550
TERFRSNIKT MYSVPNGKIH VGYKDLMENY QIVVSNLAAE RGEKDTNLVL
1560 1570 1580 1590 1600
NVFQSLLDEF TRSYNKTDFE PVKGKAQKKK RKQERAVQEH NGHVFASYQV
1610 1620 1630 1640 1650
NIPQSCEQCL SYIWLMDKAL LCSVCKMTCH KKCVHKIQSY CSYTGRRKSE
1660 1670 1680 1690 1700
LGAEPGHFGV CVDSLTSDKA SVPIVLEKLL EHVEMHGLYT EGLYRKSGAA
1710 1720 1730 1740 1750
NRTRELRQAL QTDPATVKLE DFPIHAITGV LKQWLRELPE PLMTFAQYGD
1760 1770 1780 1790 1800
FLRAVELPEK QEQLAAIYAV LDHLPEANHT SLERLIFHLV KVALLEDVNR
1810 1820 1830 1840 1850
MSPGALAIIF APCLLRCPDN SDPLTSMKDV LKITTCVEML IKEQMRKYKV
1860 1870 1880 1890 1900
KMEEINHLEA AESIAFRRLS LLRQNAPWPL KLGFSSPYEG VRTKSPRTPV
1910 1920 1930 1940 1950
VQDLEELGAL PEEAAGGDED REKEILMERI QSIKEEKEDI TYRLPELDPR
1960 1970 1980
GSDEENLDSE TSASTESLLE ERAVRGAAEE
Length:1,980
Mass (Da):225,037
Last modified:November 1, 1996 - v1
Checksum:iD79FEC4D0FAE0C05
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77609 mRNA. Translation: CAA54700.1.
PIRiS54307.
UniGeneiRn.33782.

Genome annotation databases

UCSCiRGD:3146. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77609 mRNA. Translation: CAA54700.1 .
PIRi S54307.
UniGenei Rn.33782.

3D structure databases

ProteinModelPortali Q63358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63358. 1 interaction.

PTM databases

PhosphoSitei Q63358.

Proteomic databases

PaxDbi Q63358.
PRIDEi Q63358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3146. rat.

Organism-specific databases

RGDi 3146. Myo9b.

Phylogenomic databases

eggNOGi COG5022.
HOGENOMi HOG000113707.
HOVERGENi HBG052558.
InParanoidi Q63358.

Miscellaneous databases

NextBioi 606841.
PROi Q63358.

Gene expression databases

ExpressionAtlasi Q63358. baseline.
Genevestigatori Q63358.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR13140:SF306. PTHR13140:SF306. 1 hit.
Pfami PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel type of myosin implicated in signalling by rho family GTPases."
    Reinhard J., Scheel A.A., Diekmann D., Hall A., Ruppert C., Baehler M.
    EMBO J. 14:697-704(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain stem and Spinal cord.
  2. "Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain."
    Chieregatti E., Gaertner A., Stoeffler H.-E., Baehler M.
    J. Cell Sci. 111:3597-3608(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMYO9B_RAT
AccessioniPrimary (citable) accession number: Q63358
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3