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Q63358

- MYO9B_RAT

UniProt

Q63358 - MYO9B_RAT

Protein

Unconventional myosin-IXb

Gene

Myo9b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi239 – 2468ATPSequence Analysis
    Zinc fingeri1592 – 164150Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. actin binding Source: RGD
    2. actin-dependent ATPase activity Source: RGD
    3. actin filament binding Source: RGD
    4. ADP binding Source: RGD
    5. ATPase activity Source: RGD
    6. ATP binding Source: RGD
    7. ATP-dependent protein binding Source: RGD
    8. GTPase activator activity Source: RGD
    9. microfilament motor activity Source: Ensembl
    10. protein binding Source: UniProtKB
    11. Rho GTPase activator activity Source: RGD
    12. zinc ion binding Source: RGD

    GO - Biological processi

    1. ATP catabolic process Source: RGD
    2. establishment of cell polarity Source: Ensembl
    3. lamellipodium morphogenesis Source: Ensembl
    4. macrophage chemotaxis Source: Ensembl
    5. monocyte chemotaxis Source: Ensembl
    6. Rho protein signal transduction Source: RGD

    Keywords - Molecular functioni

    GTPase activation, Motor protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Unconventional myosin-IXb
    Alternative name(s):
    Unconventional myosin-9b
    Gene namesi
    Name:Myo9b
    Synonyms:Myr5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3146. Myo9b.

    Subcellular locationi

    Cytoplasmcell cortex By similarity. Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeleton By similarity
    Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.By similarity

    GO - Cellular componenti

    1. actin filament Source: RGD
    2. cell cortex Source: UniProtKB-SubCell
    3. cytoplasm Source: RGD
    4. filamentous actin Source: Ensembl
    5. filopodium tip Source: Ensembl
    6. lamellipodium Source: RGD
    7. myosin complex Source: UniProtKB-KW
    8. perinuclear region of cytoplasm Source: RGD
    9. ruffle Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 19801979Unconventional myosin-IXbPRO_0000123471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei717 – 7171PhosphoserineBy similarity
    Modified residuei1177 – 11771PhosphoserineBy similarity
    Modified residuei1220 – 12201PhosphoserineBy similarity
    Modified residuei1243 – 12431PhosphoserineBy similarity
    Modified residuei1266 – 12661PhosphoserineBy similarity
    Modified residuei1319 – 13191PhosphothreonineBy similarity
    Modified residuei1327 – 13271PhosphoserineBy similarity
    Modified residuei1952 – 19521PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ63358.
    PRIDEiQ63358.

    PTM databases

    PhosphoSiteiQ63358.

    Expressioni

    Tissue specificityi

    Expressed in testis, lung, thymus, brain, liver, spleen and heart muscle. Detected in lung, testis, spleen and liver, and at reduced level in different brain regions (at protein level).1 Publication

    Developmental stagei

    During forebrein development, it is expressed at higher levels in embryonic and early postnatal stages than in the adult forebrain.1 Publication

    Gene expression databases

    GenevestigatoriQ63358.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Arfgef1D4A6314EBI-6251137,EBI-6251168

    Protein-protein interaction databases

    IntActiQ63358. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 114100Ras-associatingPROSITE-ProRule annotationAdd
    BLAST
    Domaini146 – 954809Myosin motorAdd
    BLAST
    Domaini958 – 97821IQ 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini981 – 100121IQ 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1002 – 102423IQ 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1025 – 105430IQ 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1663 – 1848186Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni845 – 85612Actin-bindingAdd
    BLAST
    Regioni941 – 1045105Neck or regulatory domainAdd
    BLAST
    Regioni1046 – 1980935TailAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1841 – 186121Sequence AnalysisAdd
    BLAST
    Coiled coili1918 – 194831Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 4 IQ domains.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated
    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 Ras-associating domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1592 – 164150Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5022.
    HOGENOMiHOG000113707.
    HOVERGENiHBG052558.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR028557. MYO9B.
    IPR001609. Myosin_head_motor_dom.
    IPR027417. P-loop_NTPase.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR13140:SF306. PTHR13140:SF306. 1 hit.
    PfamiPF00612. IQ. 4 hits.
    PF00063. Myosin_head. 1 hit.
    PF00788. RA. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00109. C1. 1 hit.
    SM00015. IQ. 4 hits.
    SM00242. MYSc. 1 hit.
    SM00314. RA. 1 hit.
    SM00324. RhoGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF52540. SSF52540. 3 hits.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50096. IQ. 3 hits.
    PS51456. MYOSIN_MOTOR. 1 hit.
    PS50200. RA. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63358-1 [UniParc]FASTAAdd to Basket

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    MSAHEAGSSG RRRPATFHLH IYPQLPSAGS QTSCRVTATK DSTTSDVIRD     50
    VVASLHLDGS KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQKEHP 100
    REDGYYFLLQ ERNADGSIQY LHVQLLAQPT AACRLVERGL LPRPQADFDD 150
    LCNLPELNEA NLLQSLKLRF VQQKIYTYAG SILVAINPFK FLPIYNPKYV 200
    KMYENQQLGK LEPHVFALAD VAYYTMLRKH VNQCIVISGE SGSGKTQSTN 250
    FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI 300
    QVNYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER 350
    QEFQLKQPQD YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ 400
    IFSVLSAILY LGNVTYKKRA TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV 450
    EVLTKRKTIT VNDKLILPYS LSEAITARDS MAKSLYSALF DWIVLRINHA 500
    LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA NEQLQYYFTQ 550
    HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP 600
    HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK 650
    NMDYMRPDIV ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE 700
    AGRLRAERAE KAEAGVSSPV TRSHVEELPR GANTPSEKLY RDLHNQIIKS 750
    LKGLPWQGED PRRLLQSLSR LQKPRTFFLK SKGIKQKQII PKNLLDSKSL 800
    RLIISMTLHD RTTKSLLHLH KKKKPPSISA QFQTSLNKLL EALGKAEPFF 850
    IRCIRSNAEK KELCFDDELV LQQLRYTGML ETVRIRRSGY SAKYTFQDFT 900
    EQFQVLLPKD VQPCREAIAA LLEKLQVDRQ NYQIGKTKVF LKETERQALQ 950
    ERLHGEVLRR ILLLQSWFRM VLERRHFVQM KHAALTIQAC WRSYRVRRTL 1000
    ERTRAAVYLQ AAWRGYLQRQ AYHHQRHSII RLQSLCRGHL QRRSFSQMML 1050
    EKQKAEQARE TAGAEMSEGE PSPVAAGEQP SEHPVEDPES LGVETETWMN 1100
    SKSPNGLSPK KEIPSPEMET PAQKTVPAES HEKVPSSREK RESRRQRGLE 1150
    HVERQNKHIQ SCREENSTLR EPSRKASLET GESFPEDTKE PREDGLETWT 1200
    ETAAPSCPKQ VPIVGDPPRS PSPLQRPASL DLDSRVSPVL PSSSLESPQD 1250
    EDKGENSTKV QDKPESPSGS TQIQRYQHPD TERLATAVEI WRGKKLASAM 1300
    LSQSLDLSEK PRTAGAALTP TEERRISFST SDVSKLSPVK TSTEVDGDLS 1350
    AKKPAGHKKK SEDPSAGPDA GLPTGSQGDS KSAFKRLFLH KAKDKKPSLE 1400
    GVEETEGSGG QAAQEAPARK TLDVPSSQQH RHTTGEKPLK GKKNRNRKVG 1450
    QITVSEKWRE SVFRKITNAN ELKFLDEFLL NKVNDLRSQK TPIESLFIEA 1500
    TERFRSNIKT MYSVPNGKIH VGYKDLMENY QIVVSNLAAE RGEKDTNLVL 1550
    NVFQSLLDEF TRSYNKTDFE PVKGKAQKKK RKQERAVQEH NGHVFASYQV 1600
    NIPQSCEQCL SYIWLMDKAL LCSVCKMTCH KKCVHKIQSY CSYTGRRKSE 1650
    LGAEPGHFGV CVDSLTSDKA SVPIVLEKLL EHVEMHGLYT EGLYRKSGAA 1700
    NRTRELRQAL QTDPATVKLE DFPIHAITGV LKQWLRELPE PLMTFAQYGD 1750
    FLRAVELPEK QEQLAAIYAV LDHLPEANHT SLERLIFHLV KVALLEDVNR 1800
    MSPGALAIIF APCLLRCPDN SDPLTSMKDV LKITTCVEML IKEQMRKYKV 1850
    KMEEINHLEA AESIAFRRLS LLRQNAPWPL KLGFSSPYEG VRTKSPRTPV 1900
    VQDLEELGAL PEEAAGGDED REKEILMERI QSIKEEKEDI TYRLPELDPR 1950
    GSDEENLDSE TSASTESLLE ERAVRGAAEE 1980
    Length:1,980
    Mass (Da):225,037
    Last modified:November 1, 1996 - v1
    Checksum:iD79FEC4D0FAE0C05
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77609 mRNA. Translation: CAA54700.1.
    PIRiS54307.
    UniGeneiRn.33782.

    Genome annotation databases

    UCSCiRGD:3146. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77609 mRNA. Translation: CAA54700.1 .
    PIRi S54307.
    UniGenei Rn.33782.

    3D structure databases

    ProteinModelPortali Q63358.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q63358. 1 interaction.

    PTM databases

    PhosphoSitei Q63358.

    Proteomic databases

    PaxDbi Q63358.
    PRIDEi Q63358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3146. rat.

    Organism-specific databases

    RGDi 3146. Myo9b.

    Phylogenomic databases

    eggNOGi COG5022.
    HOGENOMi HOG000113707.
    HOVERGENi HBG052558.

    Miscellaneous databases

    NextBioi 606841.
    PROi Q63358.

    Gene expression databases

    Genevestigatori Q63358.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR028557. MYO9B.
    IPR001609. Myosin_head_motor_dom.
    IPR027417. P-loop_NTPase.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR13140:SF306. PTHR13140:SF306. 1 hit.
    Pfami PF00612. IQ. 4 hits.
    PF00063. Myosin_head. 1 hit.
    PF00788. RA. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00109. C1. 1 hit.
    SM00015. IQ. 4 hits.
    SM00242. MYSc. 1 hit.
    SM00314. RA. 1 hit.
    SM00324. RhoGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF52540. SSF52540. 3 hits.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50096. IQ. 3 hits.
    PS51456. MYOSIN_MOTOR. 1 hit.
    PS50200. RA. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel type of myosin implicated in signalling by rho family GTPases."
      Reinhard J., Scheel A.A., Diekmann D., Hall A., Ruppert C., Baehler M.
      EMBO J. 14:697-704(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain stem and Spinal cord.
    2. "Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain."
      Chieregatti E., Gaertner A., Stoeffler H.-E., Baehler M.
      J. Cell Sci. 111:3597-3608(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMYO9B_RAT
    AccessioniPrimary (citable) accession number: Q63358
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3