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Q63358 (MYO9B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Unconventional myosin-IXb
Alternative name(s):
Unconventional myosin-9b
Gene names
Name:Myo9b
Synonyms:Myr5
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1980 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.

Subcellular location

Cytoplasmcell cortex By similarity. Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeleton By similarity. Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region By similarity.

Tissue specificity

Expressed in testis, lung, thymus, brain, liver, spleen and heart muscle. Detected in lung, testis, spleen and liver, and at reduced level in different brain regions (at protein level). Ref.2

Developmental stage

During forebrein development, it is expressed at higher levels in embryonic and early postnatal stages than in the adult forebrain. Ref.2

Sequence similarities

Contains 4 IQ domains.

Contains 1 myosin head-like domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 Ras-associating domain.

Contains 1 Rho-GAP domain.

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   DomainCoiled coil
Repeat
Zinc-finger
   LigandActin-binding
ATP-binding
Calmodulin-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionGTPase activation
Motor protein
Myosin
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay PubMed 16179355. Source: RGD

Rho protein signal transduction

Inferred from direct assay Ref.1. Source: RGD

establishment of cell polarity

Inferred from electronic annotation. Source: Ensembl

lamellipodium morphogenesis

Inferred from electronic annotation. Source: Ensembl

macrophage chemotaxis

Inferred from electronic annotation. Source: Ensembl

monocyte chemotaxis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentactin filament

Inferred from direct assay PubMed 9348541. Source: RGD

cell cortex

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay PubMed 9348541. Source: RGD

filamentous actin

Inferred from electronic annotation. Source: Ensembl

filopodium tip

Inferred from electronic annotation. Source: Ensembl

lamellipodium

Inferred from direct assay PubMed 9348541. Source: RGD

myosin complex

Inferred from electronic annotation. Source: UniProtKB-KW

perinuclear region of cytoplasm

Inferred from direct assay PubMed 9348541. Source: RGD

ruffle

Inferred from direct assay PubMed 9348541. Source: RGD

   Molecular_functionADP binding

Inferred from direct assay PubMed 16179355. Source: RGD

ATP binding

Inferred from direct assay PubMed 16179355. Source: RGD

ATP-dependent protein binding

Inferred from direct assay Ref.1. Source: RGD

ATPase activity

Inferred from direct assay PubMed 16179355. Source: RGD

GTPase activator activity

Inferred from direct assay Ref.1. Source: RGD

Rho GTPase activator activity

Inferred from mutant phenotype PubMed 9348541. Source: RGD

actin binding

Inferred from direct assay Ref.1. Source: RGD

actin filament binding

Inferred from direct assay PubMed 16179355Ref.1. Source: RGD

actin-dependent ATPase activity

Inferred from direct assay PubMed 16179355. Source: RGD

microfilament motor activity

Inferred from electronic annotation. Source: Ensembl

zinc ion binding

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Arfgef1D4A6314EBI-6251137,EBI-6251168

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 19801979Unconventional myosin-IXb
PRO_0000123471

Regions

Domain2 – 940939Myosin head-like
Domain15 – 114100Ras-associating
Domain958 – 97821IQ 1
Domain981 – 100121IQ 2
Domain1002 – 102423IQ 3
Domain1025 – 105430IQ 4
Domain1663 – 1848186Rho-GAP
Nucleotide binding239 – 2468ATP Potential
Zinc finger1592 – 164150Phorbol-ester/DAG-type
Region845 – 85612Actin-binding
Region941 – 1045105Neck or regulatory domain
Region1046 – 1980935Tail
Coiled coil1841 – 186121 Potential
Coiled coil1918 – 194831 Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue7171Phosphoserine By similarity
Modified residue11771Phosphoserine By similarity
Modified residue12201Phosphoserine By similarity
Modified residue12431Phosphoserine By similarity
Modified residue12661Phosphoserine By similarity
Modified residue13191Phosphothreonine By similarity
Modified residue13271Phosphoserine By similarity
Modified residue19521Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q63358 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D79FEC4D0FAE0C05

FASTA1,980225,037
        10         20         30         40         50         60 
MSAHEAGSSG RRRPATFHLH IYPQLPSAGS QTSCRVTATK DSTTSDVIRD VVASLHLDGS 

        70         80         90        100        110        120 
KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQKEHP REDGYYFLLQ ERNADGSIQY 

       130        140        150        160        170        180 
LHVQLLAQPT AACRLVERGL LPRPQADFDD LCNLPELNEA NLLQSLKLRF VQQKIYTYAG 

       190        200        210        220        230        240 
SILVAINPFK FLPIYNPKYV KMYENQQLGK LEPHVFALAD VAYYTMLRKH VNQCIVISGE 

       250        260        270        280        290        300 
SGSGKTQSTN FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI 

       310        320        330        340        350        360 
QVNYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER QEFQLKQPQD 

       370        380        390        400        410        420 
YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ IFSVLSAILY LGNVTYKKRA 

       430        440        450        460        470        480 
TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV EVLTKRKTIT VNDKLILPYS LSEAITARDS 

       490        500        510        520        530        540 
MAKSLYSALF DWIVLRINHA LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA 

       550        560        570        580        590        600 
NEQLQYYFTQ HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP 

       610        620        630        640        650        660 
HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK NMDYMRPDIV 

       670        680        690        700        710        720 
ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE AGRLRAERAE KAEAGVSSPV 

       730        740        750        760        770        780 
TRSHVEELPR GANTPSEKLY RDLHNQIIKS LKGLPWQGED PRRLLQSLSR LQKPRTFFLK 

       790        800        810        820        830        840 
SKGIKQKQII PKNLLDSKSL RLIISMTLHD RTTKSLLHLH KKKKPPSISA QFQTSLNKLL 

       850        860        870        880        890        900 
EALGKAEPFF IRCIRSNAEK KELCFDDELV LQQLRYTGML ETVRIRRSGY SAKYTFQDFT 

       910        920        930        940        950        960 
EQFQVLLPKD VQPCREAIAA LLEKLQVDRQ NYQIGKTKVF LKETERQALQ ERLHGEVLRR 

       970        980        990       1000       1010       1020 
ILLLQSWFRM VLERRHFVQM KHAALTIQAC WRSYRVRRTL ERTRAAVYLQ AAWRGYLQRQ 

      1030       1040       1050       1060       1070       1080 
AYHHQRHSII RLQSLCRGHL QRRSFSQMML EKQKAEQARE TAGAEMSEGE PSPVAAGEQP 

      1090       1100       1110       1120       1130       1140 
SEHPVEDPES LGVETETWMN SKSPNGLSPK KEIPSPEMET PAQKTVPAES HEKVPSSREK 

      1150       1160       1170       1180       1190       1200 
RESRRQRGLE HVERQNKHIQ SCREENSTLR EPSRKASLET GESFPEDTKE PREDGLETWT 

      1210       1220       1230       1240       1250       1260 
ETAAPSCPKQ VPIVGDPPRS PSPLQRPASL DLDSRVSPVL PSSSLESPQD EDKGENSTKV 

      1270       1280       1290       1300       1310       1320 
QDKPESPSGS TQIQRYQHPD TERLATAVEI WRGKKLASAM LSQSLDLSEK PRTAGAALTP 

      1330       1340       1350       1360       1370       1380 
TEERRISFST SDVSKLSPVK TSTEVDGDLS AKKPAGHKKK SEDPSAGPDA GLPTGSQGDS 

      1390       1400       1410       1420       1430       1440 
KSAFKRLFLH KAKDKKPSLE GVEETEGSGG QAAQEAPARK TLDVPSSQQH RHTTGEKPLK 

      1450       1460       1470       1480       1490       1500 
GKKNRNRKVG QITVSEKWRE SVFRKITNAN ELKFLDEFLL NKVNDLRSQK TPIESLFIEA 

      1510       1520       1530       1540       1550       1560 
TERFRSNIKT MYSVPNGKIH VGYKDLMENY QIVVSNLAAE RGEKDTNLVL NVFQSLLDEF 

      1570       1580       1590       1600       1610       1620 
TRSYNKTDFE PVKGKAQKKK RKQERAVQEH NGHVFASYQV NIPQSCEQCL SYIWLMDKAL 

      1630       1640       1650       1660       1670       1680 
LCSVCKMTCH KKCVHKIQSY CSYTGRRKSE LGAEPGHFGV CVDSLTSDKA SVPIVLEKLL 

      1690       1700       1710       1720       1730       1740 
EHVEMHGLYT EGLYRKSGAA NRTRELRQAL QTDPATVKLE DFPIHAITGV LKQWLRELPE 

      1750       1760       1770       1780       1790       1800 
PLMTFAQYGD FLRAVELPEK QEQLAAIYAV LDHLPEANHT SLERLIFHLV KVALLEDVNR 

      1810       1820       1830       1840       1850       1860 
MSPGALAIIF APCLLRCPDN SDPLTSMKDV LKITTCVEML IKEQMRKYKV KMEEINHLEA 

      1870       1880       1890       1900       1910       1920 
AESIAFRRLS LLRQNAPWPL KLGFSSPYEG VRTKSPRTPV VQDLEELGAL PEEAAGGDED 

      1930       1940       1950       1960       1970       1980 
REKEILMERI QSIKEEKEDI TYRLPELDPR GSDEENLDSE TSASTESLLE ERAVRGAAEE 

« Hide

References

[1]"A novel type of myosin implicated in signalling by rho family GTPases."
Reinhard J., Scheel A.A., Diekmann D., Hall A., Ruppert C., Baehler M.
EMBO J. 14:697-704(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain stem and Spinal cord.
[2]"Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain."
Chieregatti E., Gaertner A., Stoeffler H.-E., Baehler M.
J. Cell Sci. 111:3597-3608(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77609 mRNA. Translation: CAA54700.1.
PIRS54307.
UniGeneRn.33782.

3D structure databases

ProteinModelPortalQ63358.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63358. 1 interaction.

PTM databases

PhosphoSiteQ63358.

Proteomic databases

PaxDbQ63358.
PRIDEQ63358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3146. rat.

Organism-specific databases

RGD3146. Myo9b.

Phylogenomic databases

eggNOGCOG5022.
HOGENOMHOG000113707.
HOVERGENHBG052558.

Gene expression databases

GenevestigatorQ63358.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PANTHERPTHR13140:SF184. PTHR13140:SF184. 1 hit.
PfamPF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
PROSITEPS50096. IQ. 3 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio606841.
PROQ63358.

Entry information

Entry nameMYO9B_RAT
AccessionPrimary (citable) accession number: Q63358
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families