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Protein

Unconventional myosin-IXb

Gene

Myo9b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (By similarity). Also acts as a GTPase activator for RHOA (PubMed:7882973). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity).By similarity1 Publication

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi239 – 246ATPSequence analysis8
Zinc fingeri1592 – 1641Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • actin binding Source: RGD
  • actin-dependent ATPase activity Source: RGD
  • actin filament binding Source: RGD
  • ADP binding Source: RGD
  • ATPase activity Source: RGD
  • ATP binding Source: RGD
  • ATP-dependent protein binding Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • GTPase activator activity Source: RGD
  • microfilament motor activity Source: UniProtKB
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: InterPro
  • zinc ion binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, GTPase activation, Motor protein, Myosin
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-194840 Rho GTPase cycle
R-RNO-2029482 Regulation of actin dynamics for phagocytic cup formation
R-RNO-8985586 SLIT2:ROBO1 increases RHOA activity

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-IXb
Alternative name(s):
Unconventional myosin-9b
Gene namesi
Name:Myo9b
Synonyms:Myr51 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi3146 Myo9b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001234712 – 1980Unconventional myosin-IXbAdd BLAST1979

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei717PhosphoserineBy similarity1
Modified residuei718PhosphoserineBy similarity1
Modified residuei1046PhosphoserineCombined sources1
Modified residuei1108PhosphoserineBy similarity1
Modified residuei1115PhosphoserineBy similarity1
Modified residuei1177PhosphoserineCombined sources1
Modified residuei1220PhosphoserineCombined sources1
Modified residuei1222PhosphoserineCombined sources1
Modified residuei1229PhosphoserineCombined sources1
Modified residuei1237PhosphoserineCombined sources1
Modified residuei1243PhosphoserineBy similarity1
Modified residuei1247PhosphoserineCombined sources1
Modified residuei1266PhosphoserineCombined sources1
Modified residuei1268PhosphoserineBy similarity1
Modified residuei1304PhosphoserineBy similarity1
Modified residuei1319PhosphothreonineCombined sources1
Modified residuei1327PhosphoserineBy similarity1
Modified residuei1329PhosphoserineBy similarity1
Modified residuei1337PhosphoserineBy similarity1
Modified residuei1649PhosphoserineBy similarity1
Modified residuei1886PhosphoserineBy similarity1
Modified residuei1932PhosphoserineBy similarity1
Modified residuei1952PhosphoserineCombined sources1
Modified residuei1959PhosphoserineCombined sources1
Modified residuei1965PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63358
PRIDEiQ63358

PTM databases

iPTMnetiQ63358
PhosphoSitePlusiQ63358

Expressioni

Tissue specificityi

Expressed in testis, lung, thymus, brain, liver, spleen and heart muscle. Detected in lung, testis, spleen and liver, and at reduced level in different brain regions (at protein level).1 Publication

Developmental stagei

During forebrein development, it is expressed at higher levels in embryonic and early postnatal stages than in the adult forebrain.1 Publication

Gene expression databases

BgeeiENSRNOG00000016256
ExpressionAtlasiQ63358 baseline and differential
GenevisibleiQ63358 RN

Interactioni

Subunit structurei

Interacts (via IQ domains) with CALM. Interacts with RHOA. Interacts (via Rho-GAP domain) with ROBO1; this inhibits the interaction with RHOA and the stimulation of RHOA GTPase activity, and thereby increases the levels of active RHOA.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Arfgef1D4A6314EBI-6251137,EBI-6251168

GO - Molecular functioni

  • actin binding Source: RGD
  • actin filament binding Source: RGD
  • ATP-dependent protein binding Source: RGD
  • calmodulin binding Source: UniProtKB-KW
  • microtubule binding Source: InterPro

Protein-protein interaction databases

IntActiQ63358, 1 interactor
STRINGi10116.ENSRNOP00000048368

Structurei

3D structure databases

ProteinModelPortaliQ63358
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 114Ras-associatingPROSITE-ProRule annotationAdd BLAST100
Domaini146 – 954Myosin motorPROSITE-ProRule annotationAdd BLAST809
Domaini958 – 978IQ 1PROSITE-ProRule annotationAdd BLAST21
Domaini981 – 1001IQ 2PROSITE-ProRule annotationAdd BLAST21
Domaini1002 – 1024IQ 3PROSITE-ProRule annotationAdd BLAST23
Domaini1025 – 1054IQ 4PROSITE-ProRule annotationAdd BLAST30
Domaini1663 – 1848Rho-GAPPROSITE-ProRule annotationAdd BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni845 – 856Actin-bindingAdd BLAST12
Regioni941 – 1045Neck or regulatory domainAdd BLAST105
Regioni1046 – 1980TailAdd BLAST935
Regioni1699 – 1704Interaction with RHOABy similarity6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1841 – 1861Sequence analysisAdd BLAST21
Coiled coili1918 – 1948Sequence analysisAdd BLAST31

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1592 – 1641Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1453 Eukaryota
KOG4229 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00900000140810
HOGENOMiHOG000113707
HOVERGENiHBG052558
InParanoidiQ63358

Family and domain databases

CDDicd00029 C1, 1 hit
cd01385 MYSc_Myo9, 1 hit
Gene3Di1.10.555.10, 1 hit
3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR028557 MYO9B
IPR001609 Myosin_head_motor_dom
IPR036023 MYSc_Myo9
IPR027417 P-loop_NTPase
IPR002219 PE/DAG-bd
IPR000159 RA_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR13140:SF306 PTHR13140:SF306, 1 hit
PfamiView protein in Pfam
PF00612 IQ, 4 hits
PF00063 Myosin_head, 2 hits
PF00788 RA, 1 hit
PF00620 RhoGAP, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00015 IQ, 4 hits
SM00242 MYSc, 1 hit
SM00314 RA, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
SSF52540 SSF52540, 3 hits
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 3 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS50200 RA, 1 hit
PS50238 RHOGAP, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHEAGSSG RRRPATFHLH IYPQLPSAGS QTSCRVTATK DSTTSDVIRD
60 70 80 90 100
VVASLHLDGS KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQKEHP
110 120 130 140 150
REDGYYFLLQ ERNADGSIQY LHVQLLAQPT AACRLVERGL LPRPQADFDD
160 170 180 190 200
LCNLPELNEA NLLQSLKLRF VQQKIYTYAG SILVAINPFK FLPIYNPKYV
210 220 230 240 250
KMYENQQLGK LEPHVFALAD VAYYTMLRKH VNQCIVISGE SGSGKTQSTN
260 270 280 290 300
FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI
310 320 330 340 350
QVNYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER
360 370 380 390 400
QEFQLKQPQD YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ
410 420 430 440 450
IFSVLSAILY LGNVTYKKRA TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV
460 470 480 490 500
EVLTKRKTIT VNDKLILPYS LSEAITARDS MAKSLYSALF DWIVLRINHA
510 520 530 540 550
LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA NEQLQYYFTQ
560 570 580 590 600
HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP
610 620 630 640 650
HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK
660 670 680 690 700
NMDYMRPDIV ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE
710 720 730 740 750
AGRLRAERAE KAEAGVSSPV TRSHVEELPR GANTPSEKLY RDLHNQIIKS
760 770 780 790 800
LKGLPWQGED PRRLLQSLSR LQKPRTFFLK SKGIKQKQII PKNLLDSKSL
810 820 830 840 850
RLIISMTLHD RTTKSLLHLH KKKKPPSISA QFQTSLNKLL EALGKAEPFF
860 870 880 890 900
IRCIRSNAEK KELCFDDELV LQQLRYTGML ETVRIRRSGY SAKYTFQDFT
910 920 930 940 950
EQFQVLLPKD VQPCREAIAA LLEKLQVDRQ NYQIGKTKVF LKETERQALQ
960 970 980 990 1000
ERLHGEVLRR ILLLQSWFRM VLERRHFVQM KHAALTIQAC WRSYRVRRTL
1010 1020 1030 1040 1050
ERTRAAVYLQ AAWRGYLQRQ AYHHQRHSII RLQSLCRGHL QRRSFSQMML
1060 1070 1080 1090 1100
EKQKAEQARE TAGAEMSEGE PSPVAAGEQP SEHPVEDPES LGVETETWMN
1110 1120 1130 1140 1150
SKSPNGLSPK KEIPSPEMET PAQKTVPAES HEKVPSSREK RESRRQRGLE
1160 1170 1180 1190 1200
HVERQNKHIQ SCREENSTLR EPSRKASLET GESFPEDTKE PREDGLETWT
1210 1220 1230 1240 1250
ETAAPSCPKQ VPIVGDPPRS PSPLQRPASL DLDSRVSPVL PSSSLESPQD
1260 1270 1280 1290 1300
EDKGENSTKV QDKPESPSGS TQIQRYQHPD TERLATAVEI WRGKKLASAM
1310 1320 1330 1340 1350
LSQSLDLSEK PRTAGAALTP TEERRISFST SDVSKLSPVK TSTEVDGDLS
1360 1370 1380 1390 1400
AKKPAGHKKK SEDPSAGPDA GLPTGSQGDS KSAFKRLFLH KAKDKKPSLE
1410 1420 1430 1440 1450
GVEETEGSGG QAAQEAPARK TLDVPSSQQH RHTTGEKPLK GKKNRNRKVG
1460 1470 1480 1490 1500
QITVSEKWRE SVFRKITNAN ELKFLDEFLL NKVNDLRSQK TPIESLFIEA
1510 1520 1530 1540 1550
TERFRSNIKT MYSVPNGKIH VGYKDLMENY QIVVSNLAAE RGEKDTNLVL
1560 1570 1580 1590 1600
NVFQSLLDEF TRSYNKTDFE PVKGKAQKKK RKQERAVQEH NGHVFASYQV
1610 1620 1630 1640 1650
NIPQSCEQCL SYIWLMDKAL LCSVCKMTCH KKCVHKIQSY CSYTGRRKSE
1660 1670 1680 1690 1700
LGAEPGHFGV CVDSLTSDKA SVPIVLEKLL EHVEMHGLYT EGLYRKSGAA
1710 1720 1730 1740 1750
NRTRELRQAL QTDPATVKLE DFPIHAITGV LKQWLRELPE PLMTFAQYGD
1760 1770 1780 1790 1800
FLRAVELPEK QEQLAAIYAV LDHLPEANHT SLERLIFHLV KVALLEDVNR
1810 1820 1830 1840 1850
MSPGALAIIF APCLLRCPDN SDPLTSMKDV LKITTCVEML IKEQMRKYKV
1860 1870 1880 1890 1900
KMEEINHLEA AESIAFRRLS LLRQNAPWPL KLGFSSPYEG VRTKSPRTPV
1910 1920 1930 1940 1950
VQDLEELGAL PEEAAGGDED REKEILMERI QSIKEEKEDI TYRLPELDPR
1960 1970 1980
GSDEENLDSE TSASTESLLE ERAVRGAAEE
Length:1,980
Mass (Da):225,037
Last modified:November 1, 1996 - v1
Checksum:iD79FEC4D0FAE0C05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77609 mRNA Translation: CAA54700.1
PIRiS54307
UniGeneiRn.33782

Genome annotation databases

EnsembliENSRNOT00000045099; ENSRNOP00000048368; ENSRNOG00000016256
ENSRNOT00000081321; ENSRNOP00000073856; ENSRNOG00000016256
UCSCiRGD:3146 rat

Similar proteinsi

Entry informationi

Entry nameiMYO9B_RAT
AccessioniPrimary (citable) accession number: Q63358
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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