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Q63358

- MYO9B_RAT

UniProt

Q63358 - MYO9B_RAT

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Protein
Unconventional myosin-IXb
Gene
Myo9b, Myr5
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2468ATP Reviewed prediction
Zinc fingeri1592 – 164150Phorbol-ester/DAG-type
Add
BLAST

GO - Molecular functioni

  1. ADP binding Source: RGD
  2. ATP binding Source: RGD
  3. ATP-dependent protein binding Source: RGD
  4. ATPase activity Source: RGD
  5. GTPase activator activity Source: RGD
  6. Rho GTPase activator activity Source: RGD
  7. actin binding Source: RGD
  8. actin filament binding Source: RGD
  9. actin-dependent ATPase activity Source: RGD
  10. microfilament motor activity Source: Ensembl
  11. protein binding Source: UniProtKB
  12. zinc ion binding Source: RGD

GO - Biological processi

  1. ATP catabolic process Source: RGD
  2. Rho protein signal transduction Source: RGD
  3. establishment of cell polarity Source: Ensembl
  4. lamellipodium morphogenesis Source: Ensembl
  5. macrophage chemotaxis Source: Ensembl
  6. monocyte chemotaxis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-IXb
Alternative name(s):
Unconventional myosin-9b
Gene namesi
Name:Myo9b
Synonyms:Myr5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3146. Myo9b.

Subcellular locationi

Cytoplasmcell cortex By similarity. Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeleton By similarity
Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region By similarity.

GO - Cellular componenti

  1. actin filament Source: RGD
  2. cell cortex Source: UniProtKB-SubCell
  3. cytoplasm Source: RGD
  4. filamentous actin Source: Ensembl
  5. filopodium tip Source: Ensembl
  6. lamellipodium Source: RGD
  7. myosin complex Source: UniProtKB-KW
  8. perinuclear region of cytoplasm Source: RGD
  9. ruffle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 19801979Unconventional myosin-IXb
PRO_0000123471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei717 – 7171Phosphoserine By similarity
Modified residuei1177 – 11771Phosphoserine By similarity
Modified residuei1220 – 12201Phosphoserine By similarity
Modified residuei1243 – 12431Phosphoserine By similarity
Modified residuei1266 – 12661Phosphoserine By similarity
Modified residuei1319 – 13191Phosphothreonine By similarity
Modified residuei1327 – 13271Phosphoserine By similarity
Modified residuei1952 – 19521Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63358.
PRIDEiQ63358.

PTM databases

PhosphoSiteiQ63358.

Expressioni

Tissue specificityi

Expressed in testis, lung, thymus, brain, liver, spleen and heart muscle. Detected in lung, testis, spleen and liver, and at reduced level in different brain regions (at protein level).1 Publication

Developmental stagei

During forebrein development, it is expressed at higher levels in embryonic and early postnatal stages than in the adult forebrain.1 Publication

Gene expression databases

GenevestigatoriQ63358.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Arfgef1D4A6314EBI-6251137,EBI-6251168

Protein-protein interaction databases

IntActiQ63358. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ63358.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 114100Ras-associating
Add
BLAST
Domaini146 – 954809Myosin motor
Add
BLAST
Domaini958 – 97821IQ 1
Add
BLAST
Domaini981 – 100121IQ 2
Add
BLAST
Domaini1002 – 102423IQ 3
Add
BLAST
Domaini1025 – 105430IQ 4
Add
BLAST
Domaini1663 – 1848186Rho-GAP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni845 – 85612Actin-binding
Add
BLAST
Regioni941 – 1045105Neck or regulatory domain
Add
BLAST
Regioni1046 – 1980935Tail
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1841 – 186121 Reviewed prediction
Add
BLAST
Coiled coili1918 – 194831 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 4 IQ domains.
Contains 1 Rho-GAP domain.

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5022.
HOGENOMiHOG000113707.
HOVERGENiHBG052558.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13140:SF306. PTHR13140:SF306. 1 hit.
PfamiPF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63358-1 [UniParc]FASTAAdd to Basket

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MSAHEAGSSG RRRPATFHLH IYPQLPSAGS QTSCRVTATK DSTTSDVIRD     50
VVASLHLDGS KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQKEHP 100
REDGYYFLLQ ERNADGSIQY LHVQLLAQPT AACRLVERGL LPRPQADFDD 150
LCNLPELNEA NLLQSLKLRF VQQKIYTYAG SILVAINPFK FLPIYNPKYV 200
KMYENQQLGK LEPHVFALAD VAYYTMLRKH VNQCIVISGE SGSGKTQSTN 250
FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI 300
QVNYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER 350
QEFQLKQPQD YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ 400
IFSVLSAILY LGNVTYKKRA TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV 450
EVLTKRKTIT VNDKLILPYS LSEAITARDS MAKSLYSALF DWIVLRINHA 500
LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA NEQLQYYFTQ 550
HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP 600
HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK 650
NMDYMRPDIV ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE 700
AGRLRAERAE KAEAGVSSPV TRSHVEELPR GANTPSEKLY RDLHNQIIKS 750
LKGLPWQGED PRRLLQSLSR LQKPRTFFLK SKGIKQKQII PKNLLDSKSL 800
RLIISMTLHD RTTKSLLHLH KKKKPPSISA QFQTSLNKLL EALGKAEPFF 850
IRCIRSNAEK KELCFDDELV LQQLRYTGML ETVRIRRSGY SAKYTFQDFT 900
EQFQVLLPKD VQPCREAIAA LLEKLQVDRQ NYQIGKTKVF LKETERQALQ 950
ERLHGEVLRR ILLLQSWFRM VLERRHFVQM KHAALTIQAC WRSYRVRRTL 1000
ERTRAAVYLQ AAWRGYLQRQ AYHHQRHSII RLQSLCRGHL QRRSFSQMML 1050
EKQKAEQARE TAGAEMSEGE PSPVAAGEQP SEHPVEDPES LGVETETWMN 1100
SKSPNGLSPK KEIPSPEMET PAQKTVPAES HEKVPSSREK RESRRQRGLE 1150
HVERQNKHIQ SCREENSTLR EPSRKASLET GESFPEDTKE PREDGLETWT 1200
ETAAPSCPKQ VPIVGDPPRS PSPLQRPASL DLDSRVSPVL PSSSLESPQD 1250
EDKGENSTKV QDKPESPSGS TQIQRYQHPD TERLATAVEI WRGKKLASAM 1300
LSQSLDLSEK PRTAGAALTP TEERRISFST SDVSKLSPVK TSTEVDGDLS 1350
AKKPAGHKKK SEDPSAGPDA GLPTGSQGDS KSAFKRLFLH KAKDKKPSLE 1400
GVEETEGSGG QAAQEAPARK TLDVPSSQQH RHTTGEKPLK GKKNRNRKVG 1450
QITVSEKWRE SVFRKITNAN ELKFLDEFLL NKVNDLRSQK TPIESLFIEA 1500
TERFRSNIKT MYSVPNGKIH VGYKDLMENY QIVVSNLAAE RGEKDTNLVL 1550
NVFQSLLDEF TRSYNKTDFE PVKGKAQKKK RKQERAVQEH NGHVFASYQV 1600
NIPQSCEQCL SYIWLMDKAL LCSVCKMTCH KKCVHKIQSY CSYTGRRKSE 1650
LGAEPGHFGV CVDSLTSDKA SVPIVLEKLL EHVEMHGLYT EGLYRKSGAA 1700
NRTRELRQAL QTDPATVKLE DFPIHAITGV LKQWLRELPE PLMTFAQYGD 1750
FLRAVELPEK QEQLAAIYAV LDHLPEANHT SLERLIFHLV KVALLEDVNR 1800
MSPGALAIIF APCLLRCPDN SDPLTSMKDV LKITTCVEML IKEQMRKYKV 1850
KMEEINHLEA AESIAFRRLS LLRQNAPWPL KLGFSSPYEG VRTKSPRTPV 1900
VQDLEELGAL PEEAAGGDED REKEILMERI QSIKEEKEDI TYRLPELDPR 1950
GSDEENLDSE TSASTESLLE ERAVRGAAEE 1980
Length:1,980
Mass (Da):225,037
Last modified:November 1, 1996 - v1
Checksum:iD79FEC4D0FAE0C05
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77609 mRNA. Translation: CAA54700.1.
PIRiS54307.
UniGeneiRn.33782.

Genome annotation databases

UCSCiRGD:3146. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77609 mRNA. Translation: CAA54700.1 .
PIRi S54307.
UniGenei Rn.33782.

3D structure databases

ProteinModelPortali Q63358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63358. 1 interaction.

PTM databases

PhosphoSitei Q63358.

Proteomic databases

PaxDbi Q63358.
PRIDEi Q63358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3146. rat.

Organism-specific databases

RGDi 3146. Myo9b.

Phylogenomic databases

eggNOGi COG5022.
HOGENOMi HOG000113707.
HOVERGENi HBG052558.

Miscellaneous databases

NextBioi 606841.
PROi Q63358.

Gene expression databases

Genevestigatori Q63358.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000159. Ras-assoc.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR13140:SF306. PTHR13140:SF306. 1 hit.
Pfami PF00612. IQ. 4 hits.
PF00063. Myosin_head. 1 hit.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view ]
PRINTSi PR00193. MYOSINHEAVY.
SMARTi SM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel type of myosin implicated in signalling by rho family GTPases."
    Reinhard J., Scheel A.A., Diekmann D., Hall A., Ruppert C., Baehler M.
    EMBO J. 14:697-704(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain stem and Spinal cord.
  2. "Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain."
    Chieregatti E., Gaertner A., Stoeffler H.-E., Baehler M.
    J. Cell Sci. 111:3597-3608(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMYO9B_RAT
AccessioniPrimary (citable) accession number: Q63358
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi