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Q63342

- M2GD_RAT

UniProt

Q63342 - M2GD_RAT

Protein

Dimethylglycine dehydrogenase, mitochondrial

Gene

Dmgdh

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    N,N-dimethylglycine + electron-transfer flavoprotein + H2O = sarcosine + formaldehyde + reduced electron-transfer flavoprotein.

    Cofactori

    Binds 1 FAD covalently per monomer.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. aminomethyltransferase activity Source: InterPro
    2. dimethylglycine dehydrogenase activity Source: RGD
    3. flavin adenine dinucleotide binding Source: RGD
    4. folic acid binding Source: RGD

    GO - Biological processi

    1. amino-acid betaine catabolic process Source: UniProtKB-UniPathway
    2. choline catabolic process Source: RGD
    3. choline metabolic process Source: RGD
    4. glycine catabolic process Source: InterPro
    5. tetrahydrofolate interconversion Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16117.
    BRENDAi1.5.99.2. 5301.
    UniPathwayiUPA00291; UER00433.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dimethylglycine dehydrogenase, mitochondrial (EC:1.5.8.4)
    Alternative name(s):
    ME2GLYDH
    Gene namesi
    Name:Dmgdh
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620453. Dmgdh.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4343MitochondrionSequence AnalysisAdd
    BLAST
    Chaini44 – 857814Dimethylglycine dehydrogenase, mitochondrialPRO_0000010769Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841Tele-8alpha-FAD histidine
    Modified residuei107 – 1071N6-acetyllysineBy similarity
    Modified residuei141 – 1411N6-acetyllysine; alternateBy similarity
    Modified residuei141 – 1411N6-succinyllysine; alternateBy similarity
    Modified residuei161 – 1611N6-acetyllysineBy similarity
    Modified residuei216 – 2161N6-acetyllysineBy similarity
    Modified residuei310 – 3101N6-succinyllysineBy similarity
    Modified residuei312 – 3121N6-succinyllysineBy similarity
    Modified residuei328 – 3281N6-acetyllysineBy similarity
    Modified residuei353 – 3531N6-acetyllysineBy similarity
    Modified residuei427 – 4271N6-acetyllysine; alternateBy similarity
    Modified residuei427 – 4271N6-succinyllysine; alternateBy similarity
    Modified residuei469 – 4691N6-acetyllysine; alternateBy similarity
    Modified residuei469 – 4691N6-succinyllysine; alternateBy similarity
    Modified residuei516 – 5161N6-acetyllysine; alternateBy similarity
    Modified residuei516 – 5161N6-succinyllysine; alternateBy similarity
    Modified residuei648 – 6481N6-acetyllysine; alternateBy similarity
    Modified residuei648 – 6481N6-succinyllysine; alternateBy similarity
    Modified residuei757 – 7571N6-acetyllysineBy similarity
    Modified residuei786 – 7861N6-acetyllysine; alternateBy similarity
    Modified residuei786 – 7861N6-succinyllysine; alternateBy similarity
    Modified residuei788 – 7881N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ63342.
    PRIDEiQ63342.

    PTM databases

    PhosphoSiteiQ63342.

    Expressioni

    Gene expression databases

    GenevestigatoriQ63342.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63342.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvT family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0665.
    HOGENOMiHOG000251716.
    HOVERGENiHBG081945.
    InParanoidiQ63342.
    PhylomeDBiQ63342.

    Family and domain databases

    Gene3Di2.40.30.110. 1 hit.
    3.30.1360.120. 2 hits.
    InterProiIPR006076. FAD-dep_OxRdtase.
    IPR013977. GCV_T_C.
    IPR006222. GCV_T_N.
    IPR029043. GcvT/YgfZ_C.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view]
    PfamiPF01266. DAO. 1 hit.
    PF01571. GCV_T. 1 hit.
    PF08669. GCV_T_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF101790. SSF101790. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63342-1 [UniParc]FASTAAdd to Basket

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    MLRLGALRLR GLALRSSQGR PSSAGLREGQ ESPPSPPEWK DRAETVIIGG    50
    GCVGVSLAYH LAKAGMRDVV LLEKSELTAG STWHAAGLTT YFHPGINLKK 100
    IHYDSIKLYE RLEEETGQVV GFHQPGSIRL ATTPERVDEF KYQMTRTNWH 150
    ATEQYIIEPE KIHELFPLLN MDKILAGLYN PGDGHIDPYS LTMALATGAR 200
    KYGVLLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA AGFWAREVGK 250
    MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL 300
    LFGPYESQEK MKLQASWVAH GVPPGFGKEL FESDLDRITE HVEAAMEMVP 350
    VLKKADIINI VNGPITYSPD ILPMVGPHQG VRNYWVAIGF GYGIIHAGGV 400
    GKYLSDWILH GEPPFDLIEL DPNRYGKWTT TQYTEAKARE SYGFNNIVGY 450
    PKEERFAGRP TQRVSGLYKI LESKCSMGFH AGWEQPHWFY KPGQDTQYRP 500
    SFRRTNWFRP VGSEYKQVMQ RVGVIDLSPF GKFNIKGQDS TQLLDHLCAN 550
    VIPKVGFTNI SHMLTPRGRV YAELTVSHQS PGEFLLITGS GSELHDLRWI 600
    EEAAVRGGYD VEIRNITDEL GVLGVAGPYA RRVLQKLTSE DLSDDVFKFL 650
    QTKSLKISDI PVTAIRISYT GELGWELYHR REDSAALYER IMNAGQEEGI 700
    DNFGTYALNA LRLEKAFRAW GSEMNCDTNP LEAGLDYFIK LNKPADFTGK 750
    QALKQIKAKG LKRRLVCLTL ATDDVDPEGN ESVWYKGKVI GNTTSGSYSY 800
    SIQKSLAFAY VPVELSEVGQ QVEVELLGKN YPATIIQEPL VLTEPTRTRL 850
    QKDGRKS 857
    Length:857
    Mass (Da):96,048
    Last modified:November 1, 1996 - v1
    Checksum:iA6FED946B8AB9878
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55995 mRNA. Translation: CAA39468.1.
    PIRiS16133.
    UniGeneiRn.3646.

    Genome annotation databases

    UCSCiRGD:620453. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55995 mRNA. Translation: CAA39468.1 .
    PIRi S16133.
    UniGenei Rn.3646.

    3D structure databases

    ProteinModelPortali Q63342.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q63342.

    Proteomic databases

    PaxDbi Q63342.
    PRIDEi Q63342.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620453. rat.

    Organism-specific databases

    RGDi 620453. Dmgdh.

    Phylogenomic databases

    eggNOGi COG0665.
    HOGENOMi HOG000251716.
    HOVERGENi HBG081945.
    InParanoidi Q63342.
    PhylomeDBi Q63342.

    Enzyme and pathway databases

    UniPathwayi UPA00291 ; UER00433 .
    BioCyci MetaCyc:MONOMER-16117.
    BRENDAi 1.5.99.2. 5301.

    Miscellaneous databases

    PROi Q63342.

    Gene expression databases

    Genevestigatori Q63342.

    Family and domain databases

    Gene3Di 2.40.30.110. 1 hit.
    3.30.1360.120. 2 hits.
    InterProi IPR006076. FAD-dep_OxRdtase.
    IPR013977. GCV_T_C.
    IPR006222. GCV_T_N.
    IPR029043. GcvT/YgfZ_C.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view ]
    Pfami PF01266. DAO. 1 hit.
    PF01571. GCV_T. 1 hit.
    PF08669. GCV_T_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101790. SSF101790. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone."
      Lang H., Polster M., Brandsch R.
      Eur. J. Biochem. 198:793-799(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    2. "The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria."
      Cook R.J., Misono K.S., Wagner C.
      J. Biol. Chem. 260:12998-13002(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: FAD-BINDING.
      Tissue: Liver.

    Entry informationi

    Entry nameiM2GD_RAT
    AccessioniPrimary (citable) accession number: Q63342
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3