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Q63342

- M2GD_RAT

UniProt

Q63342 - M2GD_RAT

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Protein

Dimethylglycine dehydrogenase, mitochondrial

Gene

Dmgdh

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

N,N-dimethylglycine + electron-transfer flavoprotein + H2O = sarcosine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactori

Binds 1 FAD covalently per monomer.1 Publication

Pathwayi

GO - Molecular functioni

  1. aminomethyltransferase activity Source: InterPro
  2. dimethylglycine dehydrogenase activity Source: RGD
  3. flavin adenine dinucleotide binding Source: RGD
  4. folic acid binding Source: RGD

GO - Biological processi

  1. amino-acid betaine catabolic process Source: UniProtKB-UniPathway
  2. choline catabolic process Source: RGD
  3. choline metabolic process Source: RGD
  4. glycine catabolic process Source: InterPro
  5. tetrahydrofolate interconversion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16117.
BRENDAi1.5.99.2. 5301.
UniPathwayiUPA00291; UER00433.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylglycine dehydrogenase, mitochondrial (EC:1.5.8.4)
Alternative name(s):
ME2GLYDH
Gene namesi
Name:Dmgdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620453. Dmgdh.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343MitochondrionSequence AnalysisAdd
BLAST
Chaini44 – 857814Dimethylglycine dehydrogenase, mitochondrialPRO_0000010769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841Tele-8alpha-FAD histidine
Modified residuei107 – 1071N6-acetyllysineBy similarity
Modified residuei141 – 1411N6-acetyllysine; alternateBy similarity
Modified residuei141 – 1411N6-succinyllysine; alternateBy similarity
Modified residuei161 – 1611N6-acetyllysineBy similarity
Modified residuei216 – 2161N6-acetyllysineBy similarity
Modified residuei310 – 3101N6-succinyllysineBy similarity
Modified residuei312 – 3121N6-succinyllysineBy similarity
Modified residuei328 – 3281N6-acetyllysineBy similarity
Modified residuei353 – 3531N6-acetyllysineBy similarity
Modified residuei427 – 4271N6-acetyllysine; alternateBy similarity
Modified residuei427 – 4271N6-succinyllysine; alternateBy similarity
Modified residuei469 – 4691N6-acetyllysine; alternateBy similarity
Modified residuei469 – 4691N6-succinyllysine; alternateBy similarity
Modified residuei516 – 5161N6-acetyllysine; alternateBy similarity
Modified residuei516 – 5161N6-succinyllysine; alternateBy similarity
Modified residuei648 – 6481N6-acetyllysine; alternateBy similarity
Modified residuei648 – 6481N6-succinyllysine; alternateBy similarity
Modified residuei757 – 7571N6-acetyllysineBy similarity
Modified residuei786 – 7861N6-acetyllysine; alternateBy similarity
Modified residuei786 – 7861N6-succinyllysine; alternateBy similarity
Modified residuei788 – 7881N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63342.
PRIDEiQ63342.

PTM databases

PhosphoSiteiQ63342.

Expressioni

Gene expression databases

GenevestigatoriQ63342.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P9SX-ray2.32A/B22-857[»]
4PAAX-ray2.26A/B22-857[»]
4PABX-ray1.85A/B2-857[»]
ProteinModelPortaliQ63342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0665.
HOGENOMiHOG000251716.
HOVERGENiHBG081945.
InParanoidiQ63342.
PhylomeDBiQ63342.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63342 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRLGALRLR GLALRSSQGR PSSAGLREGQ ESPPSPPEWK DRAETVIIGG
60 70 80 90 100
GCVGVSLAYH LAKAGMRDVV LLEKSELTAG STWHAAGLTT YFHPGINLKK
110 120 130 140 150
IHYDSIKLYE RLEEETGQVV GFHQPGSIRL ATTPERVDEF KYQMTRTNWH
160 170 180 190 200
ATEQYIIEPE KIHELFPLLN MDKILAGLYN PGDGHIDPYS LTMALATGAR
210 220 230 240 250
KYGVLLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA AGFWAREVGK
260 270 280 290 300
MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL
310 320 330 340 350
LFGPYESQEK MKLQASWVAH GVPPGFGKEL FESDLDRITE HVEAAMEMVP
360 370 380 390 400
VLKKADIINI VNGPITYSPD ILPMVGPHQG VRNYWVAIGF GYGIIHAGGV
410 420 430 440 450
GKYLSDWILH GEPPFDLIEL DPNRYGKWTT TQYTEAKARE SYGFNNIVGY
460 470 480 490 500
PKEERFAGRP TQRVSGLYKI LESKCSMGFH AGWEQPHWFY KPGQDTQYRP
510 520 530 540 550
SFRRTNWFRP VGSEYKQVMQ RVGVIDLSPF GKFNIKGQDS TQLLDHLCAN
560 570 580 590 600
VIPKVGFTNI SHMLTPRGRV YAELTVSHQS PGEFLLITGS GSELHDLRWI
610 620 630 640 650
EEAAVRGGYD VEIRNITDEL GVLGVAGPYA RRVLQKLTSE DLSDDVFKFL
660 670 680 690 700
QTKSLKISDI PVTAIRISYT GELGWELYHR REDSAALYER IMNAGQEEGI
710 720 730 740 750
DNFGTYALNA LRLEKAFRAW GSEMNCDTNP LEAGLDYFIK LNKPADFTGK
760 770 780 790 800
QALKQIKAKG LKRRLVCLTL ATDDVDPEGN ESVWYKGKVI GNTTSGSYSY
810 820 830 840 850
SIQKSLAFAY VPVELSEVGQ QVEVELLGKN YPATIIQEPL VLTEPTRTRL

QKDGRKS
Length:857
Mass (Da):96,048
Last modified:November 1, 1996 - v1
Checksum:iA6FED946B8AB9878
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55995 mRNA. Translation: CAA39468.1.
PIRiS16133.
UniGeneiRn.3646.

Genome annotation databases

UCSCiRGD:620453. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55995 mRNA. Translation: CAA39468.1 .
PIRi S16133.
UniGenei Rn.3646.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4P9S X-ray 2.32 A/B 22-857 [» ]
4PAA X-ray 2.26 A/B 22-857 [» ]
4PAB X-ray 1.85 A/B 2-857 [» ]
ProteinModelPortali Q63342.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q63342.

Proteomic databases

PaxDbi Q63342.
PRIDEi Q63342.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620453. rat.

Organism-specific databases

RGDi 620453. Dmgdh.

Phylogenomic databases

eggNOGi COG0665.
HOGENOMi HOG000251716.
HOVERGENi HBG081945.
InParanoidi Q63342.
PhylomeDBi Q63342.

Enzyme and pathway databases

UniPathwayi UPA00291 ; UER00433 .
BioCyci MetaCyc:MONOMER-16117.
BRENDAi 1.5.99.2. 5301.

Miscellaneous databases

PROi Q63342.

Gene expression databases

Genevestigatori Q63342.

Family and domain databases

Gene3Di 2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProi IPR006076. FAD-dep_OxRdtase.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view ]
Pfami PF01266. DAO. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view ]
SUPFAMi SSF101790. SSF101790. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone."
    Lang H., Polster M., Brandsch R.
    Eur. J. Biochem. 198:793-799(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria."
    Cook R.J., Misono K.S., Wagner C.
    J. Biol. Chem. 260:12998-13002(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FAD-BINDING.
    Tissue: Liver.

Entry informationi

Entry nameiM2GD_RAT
AccessioniPrimary (citable) accession number: Q63342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3