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Protein

Dimethylglycine dehydrogenase, mitochondrial

Gene

Dmgdh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N,N-dimethylglycine + electron-transfer flavoprotein + H2O = sarcosine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactori

FAD1 PublicationNote: Binds 1 FAD covalently per monomer.1 Publication

Pathwayi: betaine degradation

This protein is involved in step 2 of the subpathway that synthesizes sarcosine from betaine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Betaine--homocysteine S-methyltransferase 1 (Bhmt)
  2. Dimethylglycine dehydrogenase, mitochondrial (Dmgdh)
This subpathway is part of the pathway betaine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sarcosine from betaine, the pathway betaine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

  • dimethylglycine dehydrogenase activity Source: RGD
  • flavin adenine dinucleotide binding Source: RGD
  • folic acid binding Source: RGD

GO - Biological processi

  • amino-acid betaine catabolic process Source: UniProtKB-UniPathway
  • choline catabolic process Source: RGD
  • choline metabolic process Source: RGD
  • tetrahydrofolate interconversion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16117.
BRENDAi1.5.8.4. 5301.
UniPathwayiUPA00291; UER00433.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylglycine dehydrogenase, mitochondrial (EC:1.5.8.4)
Alternative name(s):
ME2GLYDH
Gene namesi
Name:Dmgdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620453. Dmgdh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionSequence analysisAdd BLAST43
ChainiPRO_000001076944 – 857Dimethylglycine dehydrogenase, mitochondrialAdd BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84Tele-8alpha-FAD histidine1
Modified residuei107N6-acetyllysineBy similarity1
Modified residuei141N6-acetyllysine; alternateBy similarity1
Modified residuei141N6-succinyllysine; alternateBy similarity1
Modified residuei161N6-acetyllysineBy similarity1
Modified residuei216N6-acetyllysineBy similarity1
Modified residuei310N6-succinyllysineBy similarity1
Modified residuei312N6-succinyllysineBy similarity1
Modified residuei328N6-acetyllysineBy similarity1
Modified residuei353N6-acetyllysineBy similarity1
Modified residuei427N6-acetyllysine; alternateBy similarity1
Modified residuei427N6-succinyllysine; alternateBy similarity1
Modified residuei469N6-acetyllysine; alternateBy similarity1
Modified residuei469N6-succinyllysine; alternateBy similarity1
Modified residuei516N6-acetyllysine; alternateBy similarity1
Modified residuei516N6-succinyllysine; alternateBy similarity1
Modified residuei648N6-acetyllysine; alternateBy similarity1
Modified residuei648N6-succinyllysine; alternateBy similarity1
Modified residuei757N6-acetyllysineBy similarity1
Modified residuei786N6-acetyllysine; alternateBy similarity1
Modified residuei786N6-succinyllysine; alternateBy similarity1
Modified residuei788N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63342.
PRIDEiQ63342.

PTM databases

iPTMnetiQ63342.
PhosphoSitePlusiQ63342.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030481.

Structurei

Secondary structure

1857
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 48Combined sources8
Helixi52 – 63Combined sources12
Beta strandi68 – 72Combined sources5
Beta strandi74 – 76Combined sources3
Turni77 – 81Combined sources5
Helixi82 – 84Combined sources3
Beta strandi94 – 96Combined sources3
Helixi98 – 116Combined sources19
Beta strandi127 – 131Combined sources5
Helixi134 – 147Combined sources14
Beta strandi150 – 152Combined sources3
Beta strandi155 – 157Combined sources3
Helixi159 – 165Combined sources7
Beta strandi176 – 180Combined sources5
Helixi188 – 201Combined sources14
Beta strandi205 – 209Combined sources5
Beta strandi214 – 217Combined sources4
Beta strandi223 – 227Combined sources5
Beta strandi230 – 239Combined sources10
Helixi242 – 244Combined sources3
Helixi245 – 250Combined sources6
Turni251 – 253Combined sources3
Beta strandi259 – 268Combined sources10
Helixi272 – 275Combined sources4
Beta strandi283 – 286Combined sources4
Turni287 – 290Combined sources4
Beta strandi291 – 296Combined sources6
Beta strandi299 – 304Combined sources6
Turni308 – 310Combined sources3
Helixi315 – 320Combined sources6
Helixi336 – 338Combined sources3
Helixi339 – 348Combined sources10
Helixi350 – 353Combined sources4
Beta strandi357 – 367Combined sources11
Beta strandi374 – 377Combined sources4
Beta strandi384 – 388Combined sources5
Helixi393 – 410Combined sources18
Helixi418 – 420Combined sources3
Helixi422 – 424Combined sources3
Helixi431 – 443Combined sources13
Turni444 – 446Combined sources3
Helixi467 – 471Combined sources5
Turni472 – 474Combined sources3
Beta strandi475 – 480Combined sources6
Beta strandi483 – 489Combined sources7
Helixi508 – 520Combined sources13
Beta strandi523 – 526Combined sources4
Beta strandi530 – 537Combined sources8
Helixi540 – 547Combined sources8
Beta strandi548 – 550Combined sources3
Beta strandi557 – 564Combined sources8
Beta strandi570 – 580Combined sources11
Beta strandi583 – 588Combined sources6
Helixi590 – 592Combined sources3
Helixi593 – 606Combined sources14
Beta strandi612 – 615Combined sources4
Turni617 – 619Combined sources3
Beta strandi620 – 627Combined sources8
Helixi630 – 635Combined sources6
Turni644 – 646Combined sources3
Beta strandi651 – 657Combined sources7
Beta strandi660 – 665Combined sources6
Beta strandi671 – 680Combined sources10
Helixi681 – 683Combined sources3
Helixi684 – 694Combined sources11
Turni695 – 699Combined sources5
Helixi705 – 714Combined sources10
Turni720 – 722Combined sources3
Turni730 – 734Combined sources5
Helixi736 – 738Combined sources3
Beta strandi743 – 745Combined sources3
Helixi750 – 759Combined sources10
Beta strandi762 – 770Combined sources9
Beta strandi782 – 785Combined sources4
Beta strandi788 – 799Combined sources12
Turni800 – 803Combined sources4
Beta strandi804 – 812Combined sources9
Helixi813 – 815Combined sources3
Beta strandi821 – 826Combined sources6
Beta strandi829 – 835Combined sources7
Helixi844 – 857Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P9SX-ray2.32A/B22-857[»]
4PAAX-ray2.26A/B22-857[»]
4PABX-ray1.85A/B2-857[»]
ProteinModelPortaliQ63342.
SMRiQ63342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
COG0665. LUCA.
HOGENOMiHOG000251716.
HOVERGENiHBG081945.
InParanoidiQ63342.
PhylomeDBiQ63342.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLGALRLR GLALRSSQGR PSSAGLREGQ ESPPSPPEWK DRAETVIIGG
60 70 80 90 100
GCVGVSLAYH LAKAGMRDVV LLEKSELTAG STWHAAGLTT YFHPGINLKK
110 120 130 140 150
IHYDSIKLYE RLEEETGQVV GFHQPGSIRL ATTPERVDEF KYQMTRTNWH
160 170 180 190 200
ATEQYIIEPE KIHELFPLLN MDKILAGLYN PGDGHIDPYS LTMALATGAR
210 220 230 240 250
KYGVLLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA AGFWAREVGK
260 270 280 290 300
MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL
310 320 330 340 350
LFGPYESQEK MKLQASWVAH GVPPGFGKEL FESDLDRITE HVEAAMEMVP
360 370 380 390 400
VLKKADIINI VNGPITYSPD ILPMVGPHQG VRNYWVAIGF GYGIIHAGGV
410 420 430 440 450
GKYLSDWILH GEPPFDLIEL DPNRYGKWTT TQYTEAKARE SYGFNNIVGY
460 470 480 490 500
PKEERFAGRP TQRVSGLYKI LESKCSMGFH AGWEQPHWFY KPGQDTQYRP
510 520 530 540 550
SFRRTNWFRP VGSEYKQVMQ RVGVIDLSPF GKFNIKGQDS TQLLDHLCAN
560 570 580 590 600
VIPKVGFTNI SHMLTPRGRV YAELTVSHQS PGEFLLITGS GSELHDLRWI
610 620 630 640 650
EEAAVRGGYD VEIRNITDEL GVLGVAGPYA RRVLQKLTSE DLSDDVFKFL
660 670 680 690 700
QTKSLKISDI PVTAIRISYT GELGWELYHR REDSAALYER IMNAGQEEGI
710 720 730 740 750
DNFGTYALNA LRLEKAFRAW GSEMNCDTNP LEAGLDYFIK LNKPADFTGK
760 770 780 790 800
QALKQIKAKG LKRRLVCLTL ATDDVDPEGN ESVWYKGKVI GNTTSGSYSY
810 820 830 840 850
SIQKSLAFAY VPVELSEVGQ QVEVELLGKN YPATIIQEPL VLTEPTRTRL

QKDGRKS
Length:857
Mass (Da):96,048
Last modified:November 1, 1996 - v1
Checksum:iA6FED946B8AB9878
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55995 mRNA. Translation: CAA39468.1.
PIRiS16133.
UniGeneiRn.3646.

Genome annotation databases

UCSCiRGD:620453. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55995 mRNA. Translation: CAA39468.1.
PIRiS16133.
UniGeneiRn.3646.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P9SX-ray2.32A/B22-857[»]
4PAAX-ray2.26A/B22-857[»]
4PABX-ray1.85A/B2-857[»]
ProteinModelPortaliQ63342.
SMRiQ63342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000030481.

PTM databases

iPTMnetiQ63342.
PhosphoSitePlusiQ63342.

Proteomic databases

PaxDbiQ63342.
PRIDEiQ63342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620453. rat.

Organism-specific databases

RGDi620453. Dmgdh.

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
COG0665. LUCA.
HOGENOMiHOG000251716.
HOVERGENiHBG081945.
InParanoidiQ63342.
PhylomeDBiQ63342.

Enzyme and pathway databases

UniPathwayiUPA00291; UER00433.
BioCyciMetaCyc:MONOMER-16117.
BRENDAi1.5.8.4. 5301.

Miscellaneous databases

PROiQ63342.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiM2GD_RAT
AccessioniPrimary (citable) accession number: Q63342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.