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Q63342 (M2GD_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dimethylglycine dehydrogenase, mitochondrial

EC=1.5.8.4
Alternative name(s):
ME2GLYDH
Gene names
Name:Dmgdh
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length857 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

N,N-dimethylglycine + electron-transfer flavoprotein + H2O = sarcosine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactor

Binds 1 FAD covalently per monomer. Ref.2

Pathway

Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the GcvT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion Potential
Chain44 – 857814Dimethylglycine dehydrogenase, mitochondrial
PRO_0000010769

Amino acid modifications

Modified residue841Tele-8alpha-FAD histidine
Modified residue1071N6-acetyllysine By similarity
Modified residue1411N6-acetyllysine; alternate By similarity
Modified residue1411N6-succinyllysine; alternate By similarity
Modified residue1611N6-acetyllysine By similarity
Modified residue2161N6-acetyllysine By similarity
Modified residue3101N6-succinyllysine By similarity
Modified residue3121N6-succinyllysine By similarity
Modified residue3281N6-acetyllysine By similarity
Modified residue3531N6-acetyllysine By similarity
Modified residue4271N6-acetyllysine; alternate By similarity
Modified residue4271N6-succinyllysine; alternate By similarity
Modified residue4691N6-acetyllysine; alternate By similarity
Modified residue4691N6-succinyllysine; alternate By similarity
Modified residue5161N6-acetyllysine; alternate By similarity
Modified residue5161N6-succinyllysine; alternate By similarity
Modified residue6481N6-acetyllysine; alternate By similarity
Modified residue6481N6-succinyllysine; alternate By similarity
Modified residue7571N6-acetyllysine By similarity
Modified residue7861N6-acetyllysine; alternate By similarity
Modified residue7861N6-succinyllysine; alternate By similarity
Modified residue7881N6-succinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q63342 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A6FED946B8AB9878

FASTA85796,048
        10         20         30         40         50         60 
MLRLGALRLR GLALRSSQGR PSSAGLREGQ ESPPSPPEWK DRAETVIIGG GCVGVSLAYH 

        70         80         90        100        110        120 
LAKAGMRDVV LLEKSELTAG STWHAAGLTT YFHPGINLKK IHYDSIKLYE RLEEETGQVV 

       130        140        150        160        170        180 
GFHQPGSIRL ATTPERVDEF KYQMTRTNWH ATEQYIIEPE KIHELFPLLN MDKILAGLYN 

       190        200        210        220        230        240 
PGDGHIDPYS LTMALATGAR KYGVLLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA 

       250        260        270        280        290        300 
AGFWAREVGK MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL 

       310        320        330        340        350        360 
LFGPYESQEK MKLQASWVAH GVPPGFGKEL FESDLDRITE HVEAAMEMVP VLKKADIINI 

       370        380        390        400        410        420 
VNGPITYSPD ILPMVGPHQG VRNYWVAIGF GYGIIHAGGV GKYLSDWILH GEPPFDLIEL 

       430        440        450        460        470        480 
DPNRYGKWTT TQYTEAKARE SYGFNNIVGY PKEERFAGRP TQRVSGLYKI LESKCSMGFH 

       490        500        510        520        530        540 
AGWEQPHWFY KPGQDTQYRP SFRRTNWFRP VGSEYKQVMQ RVGVIDLSPF GKFNIKGQDS 

       550        560        570        580        590        600 
TQLLDHLCAN VIPKVGFTNI SHMLTPRGRV YAELTVSHQS PGEFLLITGS GSELHDLRWI 

       610        620        630        640        650        660 
EEAAVRGGYD VEIRNITDEL GVLGVAGPYA RRVLQKLTSE DLSDDVFKFL QTKSLKISDI 

       670        680        690        700        710        720 
PVTAIRISYT GELGWELYHR REDSAALYER IMNAGQEEGI DNFGTYALNA LRLEKAFRAW 

       730        740        750        760        770        780 
GSEMNCDTNP LEAGLDYFIK LNKPADFTGK QALKQIKAKG LKRRLVCLTL ATDDVDPEGN 

       790        800        810        820        830        840 
ESVWYKGKVI GNTTSGSYSY SIQKSLAFAY VPVELSEVGQ QVEVELLGKN YPATIIQEPL 

       850 
VLTEPTRTRL QKDGRKS 

« Hide

References

[1]"Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone."
Lang H., Polster M., Brandsch R.
Eur. J. Biochem. 198:793-799(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[2]"The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria."
Cook R.J., Misono K.S., Wagner C.
J. Biol. Chem. 260:12998-13002(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: FAD-BINDING.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55995 mRNA. Translation: CAA39468.1.
PIRS16133.
UniGeneRn.3646.

3D structure databases

ProteinModelPortalQ63342.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ63342.

Proteomic databases

PaxDbQ63342.
PRIDEQ63342.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:620453. rat.

Organism-specific databases

RGD620453. Dmgdh.

Phylogenomic databases

eggNOGCOG0665.
HOGENOMHOG000251716.
HOVERGENHBG081945.
InParanoidQ63342.
PhylomeDBQ63342.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16117.
BRENDA1.5.99.2. 5301.
UniPathwayUPA00291; UER00433.

Gene expression databases

GenevestigatorQ63342.

Family and domain databases

Gene3D2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProIPR006076. FAD-dep_OxRdtase.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ63342.

Entry information

Entry nameM2GD_RAT
AccessionPrimary (citable) accession number: Q63342
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways