Reviewed, UniProtKB/Swiss-Prot Q63342 (M2GD_RAT)

Names and origin

Protein names

Recommended name:
    Dimethylglycine dehydrogenase, mitochondrial
    EC=1.5.99.2
Alternative name(s):
    ME2GLYDH

Gene names

Name:

Dmgdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N,N-dimethylglycine + acceptor + H2O = sarcosine + formaldehyde + reduced acceptor.
Cofactor	Binds 1 FAD covalently per monomer. Ref.2
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	choline catabolic process Ref.1 Traceable author statement. Source: RGD glycine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Ref.1 Inferred from direct assay. Source: RGD
Molecular function	FAD binding Inferred from direct assay. Source: RGD aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro dimethylglycine dehydrogenase activity Ref.1 Inferred from direct assay. Source: RGD folic acid binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Potential
	Chain	44 – 857	814	Dimethylglycine dehydrogenase, mitochondrial		PRO_0000010769
Amino acid modifications
	Modified residue	84	1	Pros-8alpha-FAD histidine

Sequences

	Sequence		Length	Mass (Da)
	Q63342-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A6FED946B8AB9878	FASTA	857	96,048
	10 20 30 40 50 60 MLRLGALRLR GLALRSSQGR PSSAGLREGQ ESPPSPPEWK DRAETVIIGG GCVGVSLAYH 70 80 90 100 110 120 LAKAGMRDVV LLEKSELTAG STWHAAGLTT YFHPGINLKK IHYDSIKLYE RLEEETGQVV 130 140 150 160 170 180 GFHQPGSIRL ATTPERVDEF KYQMTRTNWH ATEQYIIEPE KIHELFPLLN MDKILAGLYN 190 200 210 220 230 240 PGDGHIDPYS LTMALATGAR KYGVLLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA 250 260 270 280 290 300 AGFWAREVGK MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL 310 320 330 340 350 360 LFGPYESQEK MKLQASWVAH GVPPGFGKEL FESDLDRITE HVEAAMEMVP VLKKADIINI 370 380 390 400 410 420 VNGPITYSPD ILPMVGPHQG VRNYWVAIGF GYGIIHAGGV GKYLSDWILH GEPPFDLIEL 430 440 450 460 470 480 DPNRYGKWTT TQYTEAKARE SYGFNNIVGY PKEERFAGRP TQRVSGLYKI LESKCSMGFH 490 500 510 520 530 540 AGWEQPHWFY KPGQDTQYRP SFRRTNWFRP VGSEYKQVMQ RVGVIDLSPF GKFNIKGQDS 550 560 570 580 590 600 TQLLDHLCAN VIPKVGFTNI SHMLTPRGRV YAELTVSHQS PGEFLLITGS GSELHDLRWI 610 620 630 640 650 660 EEAAVRGGYD VEIRNITDEL GVLGVAGPYA RRVLQKLTSE DLSDDVFKFL QTKSLKISDI 670 680 690 700 710 720 PVTAIRISYT GELGWELYHR REDSAALYER IMNAGQEEGI DNFGTYALNA LRLEKAFRAW 730 740 750 760 770 780 GSEMNCDTNP LEAGLDYFIK LNKPADFTGK QALKQIKAKG LKRRLVCLTL ATDDVDPEGN 790 800 810 820 830 840 ESVWYKGKVI GNTTSGSYSY SIQKSLAFAY VPVELSEVGQ QVEVELLGKN YPATIIQEPL 850 VLTEPTRTRL QKDGRKS

References

[1]	"Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone." Lang H., Polster M., Brandsch R. Eur. J. Biochem. 198:793-799(1991) [PubMed: 1710985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria." Cook R.J., Misono K.S., Wagner C. J. Biol. Chem. 260:12998-13002(1985) [PubMed: 4055729] [Abstract] Cited for: FAD-BINDING. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55995 mRNA. Translation: CAA39468.1.
IPI	IPI00207941.
PIR	S16133.
UniGene	Rn.3646
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q63342.
Genome annotation databases
Ensembl	ENSRNOG00000023588. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	620453. Dmgdh.
Phylogenomic databases
HOVERGEN	Q63342.
Enzyme and pathway databases
BRENDA	1.5.99.2. 248.
Gene expression databases
ArrayExpress	Q63342.
GermOnline	ENSRNOG00000023588. Rattus norvegicus.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M2GD_RAT

Accession

Primary (citable) accession number: Q63342

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)