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Q63341

- MMP12_RAT

UniProt

Q63341 - MMP12_RAT

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Protein

Macrophage metalloelastase

Gene

Mmp12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).By similarity

Catalytic activityi

Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin.

Cofactori

Binds 4 calcium ions per subunit.By similarity
Binds 2 zinc ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Zinc 2; in inhibited formBy similarity
Metal bindingi120 – 1201Calcium 1By similarity
Metal bindingi154 – 1541Calcium 2By similarity
Metal bindingi164 – 1641Zinc 1By similarity
Metal bindingi166 – 1661Zinc 1By similarity
Metal bindingi171 – 1711Calcium 3By similarity
Metal bindingi172 – 1721Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi174 – 1741Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi176 – 1761Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi179 – 1791Zinc 1By similarity
Metal bindingi186 – 1861Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi190 – 1901Calcium 2By similarity
Metal bindingi192 – 1921Zinc 1By similarity
Metal bindingi194 – 1941Calcium 3By similarity
Metal bindingi195 – 1951Calcium 1By similarity
Metal bindingi197 – 1971Calcium 1By similarity
Metal bindingi197 – 1971Calcium 3By similarity
Metal bindingi214 – 2141Zinc 2; catalyticBy similarity
Active sitei215 – 2151PROSITE-ProRule annotation
Metal bindingi218 – 2181Zinc 2; catalyticBy similarity
Metal bindingi224 – 2241Zinc 2; catalyticBy similarity
Metal bindingi285 – 2851Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi377 – 3771Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi426 – 4261Calcium 4; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of epithelial cell proliferation Source: RGD
  2. response to drug Source: RGD
  3. response to organic cyclic compound Source: RGD
  4. wound healing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage metalloelastase (EC:3.4.24.65)
Short name:
MME
Alternative name(s):
Matrix metalloproteinase-12
Short name:
MMP-12
Gene namesi
Name:Mmp12
Synonyms:Mmel
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620195. Mmp12.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121CuratedAdd
BLAST
Propeptidei22 – 10180Activation peptideBy similarityPRO_0000028782Add
BLAST
Chaini102 – 465364Macrophage metalloelastasePRO_0000028783Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi278 ↔ 465By similarity
Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ63341.

Expressioni

Gene expression databases

GenevestigatoriQ63341.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ63341.
SMRiQ63341. Positions 105-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati281 – 32444Hemopexin 1Add
BLAST
Repeati325 – 37147Hemopexin 2Add
BLAST
Repeati373 – 42149Hemopexin 3Add
BLAST
Repeati422 – 46544Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 938Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG323958.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiQ63341.
KOiK01413.
PhylomeDBiQ63341.
TreeFamiTF315428.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028718. MMP12.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF32. PTHR10201:SF32. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63341-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLLVLVLL VSLQVSACGA APMNESEFAE WYLSRFFDYQ GDRIPMTKTK
60 70 80 90 100
TNRNLLEEKL QEMQQFFGLE VTGQLDTSTL KIMHTSRCGV PDVQHLRAVP
110 120 130 140 150
QRSRWMKRYL TYRIYNYTPD MKRADVDYIF QKAFQVWSDV TPLRFRKIHK
160 170 180 190 200
GEADITILFA FGDHGDFYDF DGKGGTLAHA FYPGPGIQGD AHFDEAETWT
210 220 230 240 250
KSFQGTNLFL VAVHELGHSL GLRHSNNPKS IMYPTYRYLH PNTFRLSADD
260 270 280 290 300
IHSIQSLYGA PVKNPSLTNP GSPPSTVCHQ SLSFDAVTTV GDKIFFFKDW
310 320 330 340 350
FFWWRLPGSP ATNITSISSM WPTIPSGIQA AYEIGGRNQL FLFKDEKYWL
360 370 380 390 400
INNLVPEPHY PRSIHSLGFP ASVKKIDAAV FDPLRQKVYF FVDKQYWRYD
410 420 430 440 450
VRQELMDAAY PKLISTHFPG IRPKIDAVLY FKRHYYIFQG AYQLEYDPLL
460
DRVTKTLSST SWFGC
Length:465
Mass (Da):53,738
Last modified:November 1, 1996 - v1
Checksum:iE779B6014EC6FF68
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231R → P in AAH88120. (PubMed:15489334)Curated
Sequence conflicti451 – 4511D → H in AAH88120. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98517 mRNA. Translation: CAA67142.1.
BC088120 mRNA. Translation: AAH88120.1.
RefSeqiNP_446415.2. NM_053963.2.
UniGeneiRn.33193.

Genome annotation databases

GeneIDi117033.
KEGGirno:117033.
UCSCiRGD:620195. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98517 mRNA. Translation: CAA67142.1 .
BC088120 mRNA. Translation: AAH88120.1 .
RefSeqi NP_446415.2. NM_053963.2.
UniGenei Rn.33193.

3D structure databases

ProteinModelPortali Q63341.
SMRi Q63341. Positions 105-465.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q63341.
ChEMBLi CHEMBL5506.

Protein family/group databases

MEROPSi M10.009.

Proteomic databases

PRIDEi Q63341.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 117033.
KEGGi rno:117033.
UCSCi RGD:620195. rat.

Organism-specific databases

CTDi 4321.
RGDi 620195. Mmp12.

Phylogenomic databases

eggNOGi NOG323958.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi Q63341.
KOi K01413.
PhylomeDBi Q63341.
TreeFami TF315428.

Miscellaneous databases

NextBioi 619817.
PROi Q63341.

Gene expression databases

Genevestigatori Q63341.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028718. MMP12.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF32. PTHR10201:SF32. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cossins J., Clements J., Catlin G.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.

Entry informationi

Entry nameiMMP12_RAT
AccessioniPrimary (citable) accession number: Q63341
Secondary accession number(s): Q5I0P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3