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Protein

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene

Plod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:7578263). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (By similarity).By similarity1 Publication

Catalytic activityi

L-lysine-[procollagen] + 2-oxoglutarate + O2 = (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe2+By similarity
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi657IronPROSITE-ProRule annotation1
Metal bindingi659IronPROSITE-ProRule annotation1
Metal bindingi709IronPROSITE-ProRule annotation1
Active sitei719Sequence analysis1

GO - Molecular functioni

  • ferrous iron binding Source: RGD
  • L-ascorbic acid binding Source: RGD
  • peptide binding Source: RGD
  • procollagen-lysine 5-dioxygenase activity Source: RGD

GO - Biological processi

  • cellular response to hormone stimulus Source: RGD
  • epidermis development Source: RGD
  • hydroxylysine biosynthetic process Source: RGD
  • peptidyl-lysine hydroxylation Source: UniProtKB
  • response to hypoxia Source: RGD

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.4. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (EC:1.14.11.41 Publication)
Alternative name(s):
Lysyl hydroxylase 1
Short name:
LH1
Gene namesi
Name:Plod1
Synonyms:Plod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621382. Plod1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000002468119 – 728Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi539N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi687N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ63321.
PRIDEiQ63321.

Interactioni

Subunit structurei

Homodimer (By similarity). Identified in a complex with P3H3 and P3H4 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ63321. 1 interactor.
MINTiMINT-4997349.
STRINGi10116.ENSRNOP00000010433.

Structurei

3D structure databases

ProteinModelPortaliQ63321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini637 – 728Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST92

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1971. Eukaryota.
ENOG410Y4QU. LUCA.
HOVERGENiHBG053618.
InParanoidiQ63321.
PhylomeDBiQ63321.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR029044. Nucleotide-diphossugar_trans.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR001006. Procol_lys_dOase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
SMARTiView protein in SMART
SM00702. P4Hc. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.
PS01325. LYS_HYDROXYLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLLLLASL AWLLLAQAKD DAKLEDNLLV LTVATKETEG FRRFKRSAQF
60 70 80 90 100
FNYKIQSLGL GEDWSAAGGP SAAGGGQKVR LLKKALKKYA DKEDLVILFV
110 120 130 140 150
DSYDVVFASG PRELLKKFQQ AKSRVVFSAE ELIYPDRRLE AKYPTVPDGK
160 170 180 190 200
RFLGSGGFIG YAPSLSKLVA EWEGQDNDSD QLFYTKIFLD PEKREQINIS
210 220 230 240 250
LDHRCRIFQN LDGALDEVVL KFEMGHVRAR NLAYDTLPVV IHGNGPTKLQ
260 270 280 290 300
VNYLGNYIPR FWTFETGCTV CDEGLRSLKG IGDEALPTVL VGVFIEQPTP
310 320 330 340 350
FLSLFFRRLL HLRYPQKQMR LFIHNQEQHH KLQVEQFLAE HGGEYQSVKL
360 370 380 390 400
VGPEVRMANA DARNMGADLC RQDQTCTYYF SVDADVALTE PNSLRLLIEQ
410 420 430 440 450
NKNVIAPLMT RHGRLWSNFW GALSADGYYA RSEDYVDIVQ GRRVGVWNVP
460 470 480 490 500
YISNIYLIKG SALRAELRHV DLFHYSKLDP DMSFCANVRQ QEVFMFLTNR
510 520 530 540 550
HTFGHLLSLD NYQTTHLHND LWEVFSNPQD WKEKYIHENY TKALAGKLVE
560 570 580 590 600
TPCPDVYWFP IFTEVACDEL VEEMEHYGQW SLGDNKDNRI QGGYENVPTI
610 620 630 640 650
DIHMNQITFE REWHKFLVEY IAPLTEKLYP GYYTKAQFDL AFVVRYKPDE
660 670 680 690 700
QPSLMPHHDA STFTINIALN RVGEDYEGGG CRFLRYNCSV RAPRKGWALM
710 720
HPGRLTHYHE GLPTTKGTRY IAVSFVDP
Length:728
Mass (Da):83,612
Last modified:November 1, 1996 - v1
Checksum:i3CAE198EB0742DF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25331 mRNA. Translation: AAA41550.1.
PIRiS59964.
UniGeneiRn.4445.

Genome annotation databases

UCSCiRGD:621382. rat.

Similar proteinsi

Entry informationi

Entry nameiPLOD1_RAT
AccessioniPrimary (citable) accession number: Q63321
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome