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Protein

Serine/threonine-protein kinase Kist

Gene

Uhmk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP
Active sitei141 – 1411Proton acceptorPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • enzyme binding Source: RGD
  • protein serine/threonine kinase activity Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

  • peptidyl-serine phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Kist (EC:2.7.11.1)
Alternative name(s):
Kinase interacting with stathmin
PAM COOH-terminal interactor protein 2
Short name:
P-CIP2
U2AF homology motif kinase 1
Gene namesi
Name:Uhmk1
Synonyms:Kis, Kist
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2968. Uhmk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi54 – 541K → R: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Serine/threonine-protein kinase KistPRO_0000086779Add
BLAST

Proteomic databases

PaxDbiQ63285.

PTM databases

iPTMnetiQ63285.
PhosphoSiteiQ63285.

Expressioni

Tissue specificityi

In the embryo, preferentially expressed in the developing nervous system.1 Publication

Interactioni

Subunit structurei

Interacts with stathmin and CDKN1B/p27Kip1 (By similarity) Interacts with PAM.By similarity2 Publications

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

IntActiQ63285. 2 interactions.
STRINGi10116.ENSRNOP00000003950.

Structurei

3D structure databases

ProteinModelPortaliQ63285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 304282Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini324 – 40683RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000013166.
HOVERGENiHBG056149.
InParanoidiQ63285.
KOiK08877.
PhylomeDBiQ63285.
TreeFamiTF331856.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000719. Prot_kinase_dom.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGTPGSPP
60 70 80 90 100
GALKQFLPPG TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF
110 120 130 140 150
SPNVPSRCLL LELLDVSVSE LLVYSSHQGC SMWMIQHCAR DVLEALAFLH
160 170 180 190 200
HEGYVHADLK PRNILWSAEN ECFKLIDFGL SFKEGNQDVK YIQTDGYRAP
210 220 230 240 250
EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK LKHTVRSQEW
260 270 280 290 300
KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC
310 320 330 340 350
SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEDEY EDVVEDVKEE
360 370 380 390 400
CQKYGPVVSL LVPKENPGRG QVFVEYANAG DSKAAQKLLT GRMFDGKFVV
410
ATFYPLSAYK RGYLYQTLL
Length:419
Mass (Da):46,505
Last modified:November 1, 1996 - v1
Checksum:iD2F9B7BF8F080DF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98374 mRNA. Translation: CAA67021.1.
U70372 mRNA. Translation: AAC53031.2.
RefSeqiNP_058989.1. NM_017293.1.
UniGeneiRn.89229.

Genome annotation databases

GeneIDi246332.
KEGGirno:246332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98374 mRNA. Translation: CAA67021.1.
U70372 mRNA. Translation: AAC53031.2.
RefSeqiNP_058989.1. NM_017293.1.
UniGeneiRn.89229.

3D structure databases

ProteinModelPortaliQ63285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63285. 2 interactions.
STRINGi10116.ENSRNOP00000003950.

PTM databases

iPTMnetiQ63285.
PhosphoSiteiQ63285.

Proteomic databases

PaxDbiQ63285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246332.
KEGGirno:246332.

Organism-specific databases

CTDi127933.
RGDi2968. Uhmk1.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000013166.
HOVERGENiHBG056149.
InParanoidiQ63285.
KOiK08877.
PhylomeDBiQ63285.
TreeFamiTF331856.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Miscellaneous databases

NextBioi623773.
PROiQ63285.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000719. Prot_kinase_dom.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-54.
    Tissue: Corpus striatum.
  2. Caldwell B.D., Darlington D.N., Penzes P., Johnson R.C., Eipper B.A., Mains R.E.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme."
    Alam M.R., Caldwell B.D., Johnson R.C., Darlington D.N., Mains R.E., Eipper B.A.
    J. Biol. Chem. 271:28636-28640(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-419, INTERACTION WITH PAM.
    Tissue: Hippocampus.

Entry informationi

Entry nameiUHMK1_RAT
AccessioniPrimary (citable) accession number: Q63285
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.