Reviewed, UniProtKB/Swiss-Prot Q63285 (UHMK1_RAT)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase Kist
    EC=2.7.11.1
Alternative name(s):
    Kinase interacting with stathmin
    U2AF homology motif kinase 1
    PAM COOH-terminal interactor protein 2
      Short name=P-CIP2

Gene names

Name:	Uhmk1
Synonyms:	Kis, Kist

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with stathmin By similarity.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	In the embryo, preferentially expressed in the developing nervous system.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	peptidyl-serine phosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Ref.1 Inferred by curator. Source: UniProtKB RNA binding Ref.1 Non-traceable author statement. Source: UniProtKB protein binding Ref.3 Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pam	P97467	1	EBI-1397144,EBI-1397216	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Serine/threonine-protein kinase Kist		PRO_0000086779
Regions
	Domain	23 – 304	282	Protein kinase
	Domain	324 – 406	83	RRM
	Nucleotide binding	29 – 37	9	ATP By similarity
Sites
	Active site	141	1	Proton acceptor By similarity
	Active site	158	1	Proton acceptor By similarity
	Binding site	54	1	ATP
Experimental info
	Mutagenesis	54	1	K → R: Loss of activity

Sequences

	Sequence		Length	Mass (Da)
	Q63285-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D2F9B7BF8F080DF3	FASTA	419	46,505
	10 20 30 40 50 60 MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGTPGSPP GALKQFLPPG 70 80 90 100 110 120 TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF SPNVPSRCLL LELLDVSVSE 130 140 150 160 170 180 LLVYSSHQGC SMWMIQHCAR DVLEALAFLH HEGYVHADLK PRNILWSAEN ECFKLIDFGL 190 200 210 220 230 240 SFKEGNQDVK YIQTDGYRAP EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK 250 260 270 280 290 300 LKHTVRSQEW KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC 310 320 330 340 350 360 SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEDEY EDVVEDVKEE CQKYGPVVSL 370 380 390 400 410 LVPKENPGRG QVFVEYANAG DSKAAQKLLT GRMFDGKFVV ATFYPLSAYK RGYLYQTLL

References

[1]	"KIS is a protein kinase with an RNA recognition motif." Maucuer A., Ozon S., Manceau V., Gavet O., Lawler S., Curmi P., Sobel A. J. Biol. Chem. 272:23151-23156(1997) [PubMed: 9287318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-54. Tissue: Corpus striatum.
[2]	Caldwell B.D., Darlington D.N., Penzes P., Johnson R.C., Eipper B.A., Mains R.E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme." Alam M.R., Caldwell B.D., Johnson R.C., Darlington D.N., Mains R.E., Eipper B.A. J. Biol. Chem. 271:28636-28640(1996) [PubMed: 8910496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-419. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X98374 mRNA. Translation: CAA67021.1. U70372 mRNA. Translation: AAC53031.2.
RefSeq	NP_058989.1.
UniGene	Rn.89229
3D structure databases
HSSP	HSSP built from PDB template 1O0P based on UniProtKB P26368.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q63285.
Genome annotation databases
Ensembl	ENSRNOG00000002941. Rattus norvegicus. [Contig view]
GeneID	246332.
KEGG	rno:246332.
Organism-specific databases
RGD	2968. Uhmk1.
Phylogenomic databases
HOVERGEN	Q63285.
Gene expression databases
ArrayExpress	Q63285.
GermOnline	ENSRNOG00000002941. Rattus norvegicus.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR000504. RRM_RNP1. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	623773.

Entry information

Entry name

UHMK1_RAT

Accession

Primary (citable) accession number: Q63285

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)