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Q63285 (UHMK1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase Kist
EC=2.7.11.1
Alternative name(s):
Kinase interacting with stathmin
PAM COOH-terminal interactor protein 2
Short name=P-CIP2
U2AF homology motif kinase 1
Gene names
Name:Uhmk1
Synonyms:Kis, Kist
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA By similarity. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with stathmin By similarity. Ref.1 UniProtKB P97343

Subcellular location

Cytoplasm. Nucleus Ref.1.

Tissue specificity

In the embryo, preferentially expressed in the developing nervous system. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpeptidyl-serine phosphorylation

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular componentnucleus

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular functionATP binding

Inferred by curator Ref.1. Source: UniProtKB

RNA binding

Non-traceable author statement Ref.1. Source: UniProtKB

protein serine/threonine kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Serine/threonine-protein kinase Kist
PRO_0000086779

Regions

Domain23 – 304282Protein kinase
Domain324 – 40683RRM
Nucleotide binding29 – 379ATP By similarity

Sites

Active site1411Proton acceptor By similarity
Active site1581Proton acceptor By similarity UniProtKB Q9NR20
Binding site541ATP

Experimental info

Mutagenesis541K → R: Loss of activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q63285 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D2F9B7BF8F080DF3

FASTA41946,505
        10         20         30         40         50         60 
MAGSGCAWGA EPPRFLEAFG RLWQVQSRLG SGSSASVYRV RCCGTPGSPP GALKQFLPPG 

        70         80         90        100        110        120 
TTGAAASAAE YGFRKERAAL EQLQGHRNIV TLYGVFTIHF SPNVPSRCLL LELLDVSVSE 

       130        140        150        160        170        180 
LLVYSSHQGC SMWMIQHCAR DVLEALAFLH HEGYVHADLK PRNILWSAEN ECFKLIDFGL 

       190        200        210        220        230        240 
SFKEGNQDVK YIQTDGYRAP EAELQNCLAQ AGLQSDTECT SAVDLWSLGI ILLEMFSGMK 

       250        260        270        280        290        300 
LKHTVRSQEW KANSSAIIDH IFASKAVVNA AIPAYHLRDL IKSMLHDDPS RRIPAEMALC 

       310        320        330        340        350        360 
SPFFSIPFAP HIEDLVMLPT PVLRLLNVLD DDYLENEDEY EDVVEDVKEE CQKYGPVVSL 

       370        380        390        400        410 
LVPKENPGRG QVFVEYANAG DSKAAQKLLT GRMFDGKFVV ATFYPLSAYK RGYLYQTLL

« Hide

References

[1]"KIS is a protein kinase with an RNA recognition motif."
Maucuer A., Ozon S., Manceau V., Gavet O., Lawler S., Curmi P., Sobel A.
J. Biol. Chem. 272:23151-23156(1997) [PubMed: 9287318] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-54.
Tissue: Corpus striatum.
[2]Caldwell B.D., Darlington D.N., Penzes P., Johnson R.C., Eipper B.A., Mains R.E.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Novel proteins that interact with the COOH-terminal cytosolic routing determinants of an integral membrane peptide-processing enzyme."
Alam M.R., Caldwell B.D., Johnson R.C., Darlington D.N., Mains R.E., Eipper B.A.
J. Biol. Chem. 271:28636-28640(1996) [PubMed: 8910496] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-419.
Tissue: Hippocampus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X98374 mRNA. Translation: CAA67021.1.
U70372 mRNA. Translation: AAC53031.2.
IPIIPI00207043.
RefSeqNP_058989.1. NM_017293.1.
UniGeneRn.89229.

3D structure databases

ProteinModelPortalQ63285.
ModBaseSearch...

Protein-protein interaction databases

IntActQ63285. 2 interactions.
STRINGQ63285.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000003950; ENSRNOP00000003950; ENSRNOG00000002941.
GeneID246332.
KEGGrno:246332.
UCSCNM_017293. rat.

Organism-specific databases

CTD127933.
RGD2968. Uhmk1.

Phylogenomic databases

GeneTreeENSGT00590000083180.
HOVERGENHBG056149.
InParanoidQ63285.
OMARVRCCGN.
OrthoDBEOG4X6C8F.
PhylomeDBQ63285.

Enzyme and pathway databases

BRENDA2.7.11.1. 5301.

Gene expression databases

ArrayExpressQ63285.
GenevestigatorQ63285.
GermOnlineENSRNOG00000002941. Rattus norvegicus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000719. Prot_kinase_cat_dom.
IPR000504. RRM_dom.
IPR017442. Se/Thr_kinase-like_dom.
[Graphical view]
Gene3DG3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
KOK08877.
PfamPF00069. Pkinase. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. False negative.
PS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623773.

Entry information

Entry nameUHMK1_RAT
AccessionPrimary (citable) accession number: Q63285
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1996
Last modified: November 16, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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