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Protein

Glutamate receptor ionotropic, kainate 5

Gene

Grik5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > glutamate >> AMPA.

GO - Molecular functioni

  • extracellularly glutamate-gated ion channel activity Source: InterPro
  • glutamate receptor activity Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • kainate selective glutamate receptor activity Source: RGD
  • PDZ domain binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

  • cellular response to glucose stimulus Source: RGD
  • chemical synaptic transmission Source: RGD
  • establishment of localization in cell Source: UniProtKB
  • excitatory postsynaptic potential Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • protein retention in ER lumen Source: UniProtKB
  • receptor clustering Source: UniProtKB
  • regulation of membrane potential Source: RGD
  • regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: RGD
  • synaptic transmission, glutamatergic Source: RGD

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-451308 Activation of Ca-permeable Kainate Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, kainate 5
Short name:
GluK5
Alternative name(s):
Glutamate receptor KA-2
Short name:
KA2
Gene namesi
Name:Grik5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2735 Grik5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini15 – 544ExtracellularSequence analysisAdd BLAST530
Transmembranei545 – 565HelicalSequence analysisAdd BLAST21
Topological domaini566 – 622CytoplasmicSequence analysisAdd BLAST57
Transmembranei623 – 643HelicalSequence analysisAdd BLAST21
Topological domaini644 – 803ExtracellularSequence analysisAdd BLAST160
Transmembranei804 – 824HelicalSequence analysisAdd BLAST21
Topological domaini825 – 979CytoplasmicSequence analysisAdd BLAST155

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4041

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14Sequence analysisAdd BLAST14
ChainiPRO_000001155415 – 979Glutamate receptor ionotropic, kainate 5Add BLAST965

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 292
Disulfide bondi83 ↔ 334
Disulfide bondi165 ↔ 170
Glycosylationi219N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi271N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi285N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi322N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi372N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi394N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi400N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi407N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi414N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi478N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ63273
PRIDEiQ63273

PTM databases

iPTMnetiQ63273
PhosphoSitePlusiQ63273

Expressioni

Tissue specificityi

High expression in the cerebral cortex, pyriform cortex, caudate-putamen, hippocampal complex, medial habenulata and granule cell layer of the cerebellum. Weak expression in globus pallidus, thalamus, colliculi and the reticular thalamic nucleus.

Developmental stagei

From embryonic day 14 through postnatal day 1, high expression in spinal cord, brain and some areas of the PNS. At E17 and E19, high expression in spinal cord, mesencephalon and telencephalic structures as in olfactory neurons and in nasal epithelium. Strong expression also in the pituitary.

Gene expression databases

BgeeiENSRNOG00000020310
GenevisibleiQ63273 RN

Interactioni

Subunit structurei

Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • PDZ domain binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi246572, 1 interactor
STRINGi10116.ENSRNOP00000027578

Chemistry databases

BindingDBiQ63273

Structurei

Secondary structure

1979
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 30Combined sources8
Beta strandi34 – 37Combined sources4
Helixi40 – 53Combined sources14
Beta strandi62 – 69Combined sources8
Beta strandi71 – 73Combined sources3
Helixi75 – 85Combined sources11
Helixi86 – 88Combined sources3
Helixi100 – 113Combined sources14
Beta strandi117 – 119Combined sources3
Helixi129 – 134Combined sources6
Beta strandi136 – 139Combined sources4
Helixi142 – 155Combined sources14
Beta strandi161 – 167Combined sources7
Helixi170 – 173Combined sources4
Helixi175 – 183Combined sources9
Beta strandi184 – 186Combined sources3
Beta strandi188 – 192Combined sources5
Helixi200 – 209Combined sources10
Beta strandi212 – 218Combined sources7
Helixi220 – 232Combined sources13
Turni233 – 236Combined sources4
Beta strandi237 – 239Combined sources3
Beta strandi241 – 244Combined sources4
Helixi249 – 251Combined sources3
Turni255 – 257Combined sources3
Beta strandi263 – 268Combined sources6
Helixi277 – 288Combined sources12
Turni289 – 291Combined sources3
Helixi294 – 296Combined sources3
Helixi302 – 321Combined sources20
Turni322 – 324Combined sources3
Helixi343 – 350Combined sources8
Beta strandi355 – 357Combined sources3
Beta strandi360 – 362Combined sources3
Beta strandi374 – 381Combined sources8
Beta strandi384 – 392Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OM0X-ray1.40A20-406[»]
3OM1X-ray1.68A/B20-406[»]
3QLUX-ray2.91A/B20-406[»]
3QLVX-ray3.94A/B/E/G/I20-406[»]
ProteinModelPortaliQ63273
SMRiQ63273
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi860 – 866Poly-Arg7

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ISR4 Eukaryota
ENOG410XRUE LUCA
GeneTreeiENSGT00910000143978
HOGENOMiHOG000234371
HOVERGENiHBG051839
InParanoidiQ63273
KOiK05205
OMAiWARHYDQ
OrthoDBiEOG091G02LN
PhylomeDBiQ63273
TreeFamiTF334668

Family and domain databases

InterProiView protein in InterPro
IPR001828 ANF_lig-bd_rcpt
IPR019594 Glu/Gly-bd
IPR001508 Iono_rcpt_met
IPR001320 Iontro_rcpt
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00060 Lig_chan, 1 hit
PF10613 Lig_chan-Glu_bd, 1 hit
PRINTSiPR00177 NMDARECEPTOR
SMARTiView protein in SMART
SM00918 Lig_chan-Glu_bd, 1 hit
SM00079 PBPe, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAELLLLLI VAFANPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ
60 70 80 90 100
INGIIEVPAK ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP
110 120 130 140 150
ASASTVSHIC GEKEIPHIKV GPEETPRLQY LRFASVSLYP SNEDVSLAVS
160 170 180 190 200
RILKSFNYPS ASLICAKAEC LLRLEELVRG FLISKETLSV RMLDDSRDPT
210 220 230 240 250
PLLKEIRDDK VSTIIIDANA SISHLVLRKA SELGMTSAFY KYILTTMDFP
260 270 280 290 300
ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYPGP
310 320 330 340 350
ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL
360 370 380 390 400
RMVEYDGLTG RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN
410 420 430 440 450
ATTLDINLSQ TLANKTLVVT TILENPYVMR RPNFQALSGN ERFEGFCVDM
460 470 480 490 500
LRELAELLRF RYRLRLVEDG LYGAPEPNGS WTGMVGELIN RKADLAVAAF
510 520 530 540 550
TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD PFSPAVWLFM
560 570 580 590 600
LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV
610 620 630 640 650
GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV
660 670 680 690 700
PVESADDLAD QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV
710 720 730 740 750
FVKSTEEGIA RVLNSRYAFL LESTMNEYHR RLNCNLTQIG GLLDTKGYGI
760 770 780 790 800
GMPLGSPFRD EITLAILQLQ ENNRLEILKR KWWEGGRCPK EEDHRAKGLG
810 820 830 840 850
MENIGGIFVV LICGLIIAVF VAVMEFIWST RRSAESEEVS VCQEMLQELR
860 870 880 890 900
HAVSCRKTSR SRRRRRPGGP SRALLSLRAV REMRLSNGKL YSAGAGGDAG
910 920 930 940 950
AHGGPQRLLD DPGPPGGPRP QAPTPCTHVR VCQECRRIQA LRASGAGAPP
960 970
RGLGTPAEAT SPPRPRPGPT GPRELTEHE
Length:979
Mass (Da):109,276
Last modified:November 1, 1996 - v1
Checksum:i313E2862746474B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti887N → G (Ref. 2) Curated1
Sequence conflicti889K → R (Ref. 2) Curated1
Sequence conflicti910D → N in AAA17831 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11581 mRNA Translation: CAA77667.1
U08258 mRNA Translation: AAA17831.1
PIRiJH0592
RefSeqiNP_113696.1, NM_031508.2
UniGeneiRn.74042

Genome annotation databases

EnsembliENSRNOT00000027578; ENSRNOP00000027578; ENSRNOG00000020310
GeneIDi24407
KEGGirno:24407
UCSCiRGD:2735 rat

Similar proteinsi

Entry informationi

Entry nameiGRIK5_RAT
AccessioniPrimary (citable) accession number: Q63273
Secondary accession number(s): Q62643
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health