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Q63270 (ACOC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytoplasmic aconitate hydratase

Short name=Aconitase
EC=4.2.1.3
Alternative name(s):
Citrate hydro-lyase
Iron regulatory protein 1
Short name=IRP1
Iron-responsive element-binding protein 1
Short name=IRE-BP 1
Gene names
Name:Aco1
Synonyms:Ireb1, Irebp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length889 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding By similarity. Ref.1

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity. Ref.1

Catalytic activity

Citrate = isocitrate.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Subunit structure

Interacts (when associated with the 4Fe-4S) with FBXL5 By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
RNA-binding
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular iron ion homeostasis

Inferred from direct assay PubMed 15086359. Source: RGD

citrate metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of translation

Traceable author statement Ref.1. Source: RGD

response to iron(II) ion

Inferred from sequence or structural similarity. Source: UniProtKB

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 16144863. Source: MGI

cytoplasm

Traceable author statement Ref.1. Source: RGD

cytosol

Inferred from direct assay PubMed 12672910PubMed 9092825. Source: RGD

endoplasmic reticulum

Inferred from direct assay PubMed 16144863. Source: RGD

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 16144863. Source: RGD

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from sequence or structural similarity. Source: UniProtKB

aconitate hydratase activity

Inferred from sequence or structural similarity. Source: UniProtKB

iron-responsive element binding

Inferred from sequence or structural similarity. Source: UniProtKB

iron-sulfur cluster binding

Inferred from direct assay PubMed 9092825. Source: RGD

mRNA 5'-UTR binding

Inferred from direct assay PubMed 9092825. Source: RGD

mRNA binding

Traceable author statement Ref.1. Source: RGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 889889Cytoplasmic aconitate hydratase
PRO_0000076683

Regions

Region205 – 2073Substrate binding By similarity
Region779 – 7802Substrate binding By similarity

Sites

Metal binding4371Iron-sulfur (4Fe-4S) By similarity
Metal binding5031Iron-sulfur (4Fe-4S) By similarity
Metal binding5061Iron-sulfur (4Fe-4S) By similarity
Binding site861Substrate By similarity
Binding site5361Substrate By similarity
Binding site5411Substrate By similarity
Binding site6991Substrate By similarity

Experimental info

Sequence conflict1311H → D AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q63270 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 610486302B4362CD

FASTA88998,128
        10         20         30         40         50         60 
MKNPFAHLAE PLDPAQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAVRNC DEFLVKKNDI 

        70         80         90        100        110        120 
ENILNWSIMQ HKSIEVPFKP ARVILQDFTG VPAVVDFAAM RDAVKKLGGN PEKINPVCPA 

       130        140        150        160        170        180 
DLVIDHSIQV HFNRRADSLQ KNQDLEFERN RERFEFLKWG SQAFCNMRII PPGSGIIHQV 

       190        200        210        220        230        240 
NLEYLARVVF DQDGCYYPDS LVGTDSHTTM IDGLGVLGWG VGGIEAEAVM LGQPISMVLP 

       250        260        270        280        290        300 
QVIGYKLMGK PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC 

       310        320        330        340        350        360 
PEYGATAAFF PVDDVSIAYL VQTGREEDKV KHIKRYLQAV GMFRDFSDSS QDPDFTQVVE 

       370        380        390        400        410        420 
LDLKTVVPCC SGPKRPQDKV AVSEIEKDFE SCLGAKQGFK GFQVAPDHHN DHKTFIYNDS 

       430        440        450        460        470        480 
EFTLAHGSVV IAAITSCTNT SNPSVMLGAG LLAKKAVEAG LNVKPYVKTS LSPGSGVVTY 

       490        500        510        520        530        540 
YLRESGVMPY LSQLGFDVVG YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG 

       550        560        570        580        590        600 
RVHPNTRANY LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GQQVFLKDIW PTRDEIQEVE 

       610        620        630        640        650        660 
RKYVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE SLTLDLQPPK 

       670        680        690        700        710        720 
SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG LTPRDFNSYG SRRGNDAIMA 

       730        740        750        760        770        780 
RGTFANIRLL NKFLNKQAPQ TVHLPSGETL DVFDAAERYQ QAGLPLIVLA GKEYGSGSSR 

       790        800        810        820        830        840 
DWAAKGPFLL GIKAVLAESY ERTHCSNLVG MGVIPLEYLP GETADSLGLT GRERYTIHIP 

       850        860        870        880 
EHLKPRMKVQ IKLDTGKTFQ AVMRFDTDVE LTYFHNGGIL NYMIRKMAQ 

« Hide

References

[1]"The iron-responsive element binding protein. Purification, cloning, and regulation in rat liver."
Yu Y., Radisky E.S., Leibold E.A.
J. Biol. Chem. 267:19005-19010(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: Sprague-Dawley.
Tissue: Liver.
[2]Lubec G., Diao W.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 114-134 AND 277-293, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23874 mRNA. Translation: AAA41449.1.
PIRA44154.
RefSeqNP_059017.1. NM_017321.1.
UniGeneRn.35934.

3D structure databases

ProteinModelPortalQ63270.
SMRQ63270. Positions 3-889.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248407. 1 interaction.
MINTMINT-4569119.
STRING10116.ENSRNOP00000057501.

PTM databases

PhosphoSiteQ63270.

Proteomic databases

PaxDbQ63270.
PRIDEQ63270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID50655.
KEGGrno:50655.
UCSCRGD:2019. rat.

Organism-specific databases

CTD48.
RGD2019. Aco1.

Phylogenomic databases

eggNOGCOG1048.
HOGENOMHOG000025704.
HOVERGENHBG052147.
KOK01681.

Gene expression databases

GenevestigatorQ63270.

Family and domain databases

Gene3D3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR006249. Aconitase/Fe_reg_prot_2.
IPR015934. Aconitase/Fe_reg_prot_2/AcnD.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PTHR11670:SF1. PTHR11670:SF1. 1 hit.
PfamPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR01341. aconitase_1. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610466.
PROQ63270.

Entry information

Entry nameACOC_RAT
AccessionPrimary (citable) accession number: Q63270
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families