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Protein

Interleukin-1 beta

Gene

Il1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.By similarity

GO - Molecular functioni

  • cytokine activity Source: RGD
  • interleukin-1 receptor binding Source: GO_Central

GO - Biological processi

  • acute inflammatory response Source: RGD
  • cellular response to antibiotic Source: RGD
  • cellular response to fatty acid Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to lipid Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • chronic inflammatory response to antigenic stimulus Source: RGD
  • cytokine-mediated signaling pathway Source: GO_Central
  • fever generation Source: RGD
  • learning or memory Source: RGD
  • memory Source: RGD
  • negative regulation of branching morphogenesis of a nerve Source: RGD
  • negative regulation of gene expression Source: RGD
  • negative regulation of glutamate secretion Source: RGD
  • negative regulation of neural precursor cell proliferation Source: RGD
  • negative regulation of neurogenesis Source: RGD
  • negative regulation of neuron differentiation Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • ovulation Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of astrocyte differentiation Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of glial cell differentiation Source: RGD
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
  • positive regulation of immature T cell proliferation in thymus Source: RGD
  • positive regulation of interleukin-6 production Source: RGD
  • positive regulation of JNK cascade Source: RGD
  • positive regulation of JUN kinase activity Source: RGD
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of neutrophil chemotaxis Source: RGD
  • positive regulation of stress-activated MAPK cascade Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • response to dexamethasone Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • response to gamma radiation Source: RGD
  • response to glucocorticoid Source: RGD
  • response to heat Source: RGD
  • response to hypoxia Source: RGD
  • response to immobilization stress Source: RGD
  • response to L-ascorbic acid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to morphine Source: RGD
  • response to nutrient Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to ozone Source: RGD
  • response to peptide hormone Source: RGD
  • response to stilbenoid Source: RGD
  • response to vitamin D Source: RGD
  • social behavior Source: RGD
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Mitogen, Pyrogen

Keywords - Biological processi

Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-1 beta
Short name:
IL-1 beta
Gene namesi
Name:Il1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2891. Il1b.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Lysosome By similarity
  • Secretedexosome By similarity
  • Cytoplasmic vesicleautophagosome By similarity
  • Secreted By similarity

  • Note: The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca2+-dependent exocytosis with release of mature IL1B. 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes. However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes. 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis. 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome. These mechanisms may not be not mutually exclusive.By similarity

GO - Cellular componenti

  • autophagosome Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB-SubCell
  • extracellular space Source: RGD
  • lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000153171 – 116By similarityAdd BLAST116
ChainiPRO_0000015318117 – 268Interleukin-1 betaAdd BLAST152

Proteomic databases

PaxDbiQ63264.
PRIDEiQ63264.

PTM databases

PhosphoSitePlusiQ63264.

Interactioni

Subunit structurei

Monomer. Interacts with MEFV (By similarity).By similarity

GO - Molecular functioni

  • cytokine activity Source: RGD
  • interleukin-1 receptor binding Source: GO_Central

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006308.

Structurei

3D structure databases

ProteinModelPortaliQ63264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-1 family.Curated

Phylogenomic databases

eggNOGiENOG410IGSZ. Eukaryota.
ENOG410XU2E. LUCA.
HOGENOMiHOG000246399.
HOVERGENiHBG007328.
InParanoidiQ63264.
PhylomeDBiQ63264.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR003294. IL-1_alpha/beta.
IPR003296. IL-1_beta.
IPR020877. IL-1_CS.
IPR000975. IL-1_fam.
IPR003502. IL-1_propep.
[Graphical view]
PANTHERiPTHR10078:SF20. PTHR10078:SF20. 1 hit.
PfamiPF00340. IL1. 1 hit.
PF02394. IL1_propep. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01357. INTRLEUKN1AB.
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00253. INTERLEUKIN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVPELNCE IAAFDSEEND LFFEADRPQK IKDCFQALDL GCPDESIQLQ
60 70 80 90 100
ISQQHLDKSF RKAVSLIVAV EKLWQLPMSC PWSFQDEDPS TFFSFIFEEE
110 120 130 140 150
PVLCDSWDDD DLLVCDVPIR QLHCRLRDEQ QKCLVLSDPC ELKALHLNGQ
160 170 180 190 200
NISQQVVFSM SFVQGETSND KIPVALGLKG LNLYLSCVMK DGTPTLQLES
210 220 230 240 250
VDPKQYPKKK MEKRFVFNKI EVKTKVEFES AQFPNWYIST SQAEHRPVFL
260
GNSNGRDIVD FTMEPVSS
Length:268
Mass (Da):30,644
Last modified:November 1, 1997 - v1
Checksum:i109C19EBF69C242D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98820 mRNA. Translation: AAA41426.1.
UniGeneiRn.9869.

Genome annotation databases

UCSCiRGD:2891. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98820 mRNA. Translation: AAA41426.1.
UniGeneiRn.9869.

3D structure databases

ProteinModelPortaliQ63264.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006308.

PTM databases

PhosphoSitePlusiQ63264.

Proteomic databases

PaxDbiQ63264.
PRIDEiQ63264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2891. rat.

Organism-specific databases

RGDi2891. Il1b.

Phylogenomic databases

eggNOGiENOG410IGSZ. Eukaryota.
ENOG410XU2E. LUCA.
HOGENOMiHOG000246399.
HOVERGENiHBG007328.
InParanoidiQ63264.
PhylomeDBiQ63264.

Miscellaneous databases

PROiQ63264.

Family and domain databases

InterProiIPR008996. Cytokine_IL1-like.
IPR003294. IL-1_alpha/beta.
IPR003296. IL-1_beta.
IPR020877. IL-1_CS.
IPR000975. IL-1_fam.
IPR003502. IL-1_propep.
[Graphical view]
PANTHERiPTHR10078:SF20. PTHR10078:SF20. 1 hit.
PfamiPF00340. IL1. 1 hit.
PF02394. IL1_propep. 1 hit.
[Graphical view]
PRINTSiPR00264. INTERLEUKIN1.
PR01357. INTRLEUKN1AB.
SUPFAMiSSF50353. SSF50353. 1 hit.
PROSITEiPS00253. INTERLEUKIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIL1B_RAT
AccessioniPrimary (citable) accession number: Q63264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.