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Protein

POU domain, class 3, transcription factor 3

Gene

Pou3f3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi403 – 46260HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  2. sequence-specific DNA binding Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: RGD

GO - Biological processi

  1. metanephric ascending thin limb development Source: UniProtKB
  2. metanephric DCT cell differentiation Source: UniProtKB
  3. metanephric loop of Henle development Source: UniProtKB
  4. metanephric macula densa development Source: UniProtKB
  5. metanephric thick ascending limb development Source: UniProtKB
  6. negative regulation of apoptotic process Source: UniProtKB
  7. negative regulation of transcription, DNA-templated Source: UniProtKB
  8. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  9. nervous system development Source: UniProtKB-KW
  10. positive regulation of cell proliferation Source: UniProtKB
  11. positive regulation of gene expression Source: UniProtKB
  12. positive regulation of transcription, DNA-templated Source: UniProtKB
  13. positive regulation of transcription from RNA polymerase II promoter Source: RGD
  14. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name:
Brain-1
Short name:
Brn-1
Gene namesi
Name:Pou3f3
Synonyms:Brn-1, Brn1, Rhs2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi619768. Pou3f3.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi450 – 4512WF → CS: Abolishes synergistic action with SOX11. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497POU domain, class 3, transcription factor 3PRO_0000100730Add
BLAST

Expressioni

Tissue specificityi

Brain.

Developmental stagei

Expressed from embryonic day 11.5 into adulthood.

Gene expression databases

GenevestigatoriQ63262.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040234.

Structurei

3D structure databases

ProteinModelPortaliQ63262.
SMRiQ63262. Positions 317-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini311 – 38575POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 301274Gly-richAdd
BLAST
Compositional biasi80 – 203124Ala-richAdd
BLAST
Compositional biasi132 – 18352Pro-richAdd
BLAST
Compositional biasi269 – 30840His-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiQ63262.
KOiK09365.
PhylomeDBiQ63262.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAASNPYL PGNSLLAAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM
60 70 80 90 100
QPGSAAVTSG AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL
110 120 130 140 150
PHAAAAAAAA AAAAVEASSP WSGSAVGMAG SPQQPPRPPP PPPQGPDVKG
160 170 180 190 200
GVGREDLHAG TALHHRGPPH LGPPPPPPHQ GHPGGWGAAA AAAAAAAAAA
210 220 230 240 250
AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG GGGGGAGGGA
260 270 280 290 300
QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP
310 320 330 340 350
GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN
360 370 380 390 400
VFSQTTICRF EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ
410 420 430 440 450
GRKRKKRTSI EVSVKGALES HFLKCPKPSA QEITNLADSL QLEKEVVRVW
460 470 480 490
FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT VSADTPPPHH GLQTSVQ
Length:497
Mass (Da):50,226
Last modified:July 15, 1999 - v2
Checksum:i00640505E343ABC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001641 mRNA. Translation: CAA04893.1.
M84644 mRNA. Translation: AAA42041.1.
RefSeqiNP_620192.1. NM_138837.1.
UniGeneiRn.11354.

Genome annotation databases

GeneIDi192109.
KEGGirno:192109.
UCSCiRGD:619768. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001641 mRNA. Translation: CAA04893.1.
M84644 mRNA. Translation: AAA42041.1.
RefSeqiNP_620192.1. NM_138837.1.
UniGeneiRn.11354.

3D structure databases

ProteinModelPortaliQ63262.
SMRiQ63262. Positions 317-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192109.
KEGGirno:192109.
UCSCiRGD:619768. rat.

Organism-specific databases

CTDi5455.
RGDi619768. Pou3f3.

Phylogenomic databases

HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiQ63262.
KOiK09365.
PhylomeDBiQ63262.

Miscellaneous databases

NextBioi622677.

Gene expression databases

GenevestigatoriQ63262.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Redundancy of class III POU proteins in the oligodendrocyte lineage."
    Schreiber J., Enderich J., Sock E., Schmidt C., Richter-Landsberg C., Wegner M.
    J. Biol. Chem. 272:32286-32293(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "RHS2, a POU domain-containing gene, and its expression in developing and adult rat."
    le Moine C., Young W.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:3285-3289(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 325-449.
    Tissue: Hypothalamus.
  3. "Cooperative function of POU proteins and SOX proteins in glial cells."
    Kuhlbrodt K., Herbarth B., Sock E., Enderich J., Hermans-Borgmeyer I., Wegner M.
    J. Biol. Chem. 273:16050-16057(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 450-TRP-PHE-451.

Entry informationi

Entry nameiPO3F3_RAT
AccessioniPrimary (citable) accession number: Q63262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.