Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q63262 (PO3F3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name=Brain-1
Short name=Brn-1
Gene names
Name:Pou3f3
Synonyms:Brn-1, Brn1, Rhs2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') By similarity. Ref.3

Subcellular location

Nucleus Ref.3.

Tissue specificity

Brain.

Developmental stage

Expressed from embryonic day 11.5 into adulthood.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processNeurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmetanephric DCT cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric ascending thin limb development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric loop of Henle development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric macula densa development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric thick ascending limb development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction Ref.3. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

nervous system development

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gene expression

Inferred from mutant phenotype PubMed 15713637. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.1. Source: RGD

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 15713637. Source: UniProtKB

transcription from RNA polymerase II promoter

Inferred from direct assay Ref.3. Source: GOC

   Cellular_componentnucleus

Inferred from mutant phenotype PubMed 15713637. Source: UniProtKB

   Molecular_functionRNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.3. Source: MGI

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497POU domain, class 3, transcription factor 3
PRO_0000100730

Regions

Domain311 – 38575POU-specific
DNA binding403 – 46260Homeobox
Compositional bias28 – 301274Gly-rich
Compositional bias80 – 203124Ala-rich
Compositional bias132 – 18352Pro-rich
Compositional bias269 – 30840His-rich

Experimental info

Mutagenesis450 – 4512WF → CS: Abolishes synergistic action with SOX11. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q63262 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 00640505E343ABC2

FASTA49750,226
        10         20         30         40         50         60 
MATAASNPYL PGNSLLAAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG 

        70         80         90        100        110        120 
AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP 

       130        140        150        160        170        180 
WSGSAVGMAG SPQQPPRPPP PPPQGPDVKG GVGREDLHAG TALHHRGPPH LGPPPPPPHQ 

       190        200        210        220        230        240 
GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG 

       250        260        270        280        290        300 
GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP 

       310        320        330        340        350        360 
GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF 

       370        380        390        400        410        420 
EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES 

       430        440        450        460        470        480 
HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT 

       490 
VSADTPPPHH GLQTSVQ 

« Hide

References

[1]"Redundancy of class III POU proteins in the oligodendrocyte lineage."
Schreiber J., Enderich J., Sock E., Schmidt C., Richter-Landsberg C., Wegner M.
J. Biol. Chem. 272:32286-32293(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"RHS2, a POU domain-containing gene, and its expression in developing and adult rat."
le Moine C., Young W.S.
Proc. Natl. Acad. Sci. U.S.A. 89:3285-3289(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 325-449.
Tissue: Hypothalamus.
[3]"Cooperative function of POU proteins and SOX proteins in glial cells."
Kuhlbrodt K., Herbarth B., Sock E., Enderich J., Hermans-Borgmeyer I., Wegner M.
J. Biol. Chem. 273:16050-16057(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 450-TRP-PHE-451.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001641 mRNA. Translation: CAA04893.1.
M84644 mRNA. Translation: AAA42041.1.
RefSeqNP_620192.1. NM_138837.1.
UniGeneRn.11354.

3D structure databases

ProteinModelPortalQ63262.
SMRQ63262. Positions 317-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000040234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID192109.
KEGGrno:192109.
UCSCRGD:619768. rat.

Organism-specific databases

CTD5455.
RGD619768. Pou3f3.

Phylogenomic databases

HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidQ63262.
KOK09365.
PhylomeDBQ63262.

Gene expression databases

GenevestigatorQ63262.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622677.

Entry information

Entry namePO3F3_RAT
AccessionPrimary (citable) accession number: Q63262
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families