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Protein

Integrin alpha-7

Gene

Itga7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. Involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells (By similarity). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi328 – 336Sequence analysis9
Calcium bindingi390 – 398Sequence analysis9
Calcium bindingi448 – 456Sequence analysis9

GO - Molecular functioni

  • cell adhesion molecule binding Source: RGD
  • integrin binding Source: RGD
  • laminin binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • cell adhesion Source: RGD
  • integrin-mediated signaling pathway Source: RGD
  • regulation of cell shape Source: UniProtKB-KW
  • skeletal muscle tissue development Source: RGD

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion, Cell shape
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-3000157. Laminin interactions.
R-RNO-3000178. ECM proteoglycans.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-7
Alternative name(s):
H36-alpha7
Cleaved into the following 3 chains:
Gene namesi
Name:Itga7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi71022. Itga7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 1036ExtracellularSequence analysisAdd BLAST1003
Transmembranei1037 – 1057HelicalSequence analysisAdd BLAST21
Topological domaini1058 – 1135CytoplasmicSequence analysisAdd BLAST78

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi603R → G: Abolishes cleavage by urokinase. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33By similarityAdd BLAST33
ChainiPRO_000029312434 – 1135Integrin alpha-7Add BLAST1102
ChainiPRO_000001627334 – 910Integrin alpha-7 heavy chainSequence analysisAdd BLAST877
ChainiPRO_0000398834604 – 1135Integrin alpha-7 70 kDa formAdd BLAST532
ChainiPRO_0000016274914 – 1135Integrin alpha-7 light chainSequence analysisAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi94 ↔ 103By similarity
Disulfide bondi140 ↔ 163By similarity
Disulfide bondi184 ↔ 197By similarity
Disulfide bondi495 ↔ 502By similarity
Disulfide bondi508 ↔ 571By similarity
Disulfide bondi637 ↔ 643By similarity
Disulfide bondi736 ↔ 747By similarity
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi894 ↔ 948Interchain (between heavy and light chains)By similarity
Glycosylationi943N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi955 ↔ 960By similarity
Glycosylationi979N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi999N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.By similarity
A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei603 – 604Cleavage; by urokinase2

Keywords - PTMi

ADP-ribosylation, Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ63258.
PRIDEiQ63258.

PTM databases

iPTMnetiQ63258.
PhosphoSitePlusiQ63258.

Expressioni

Tissue specificityi

Expressed in skeletal and cardiac muscle. Expressed in replicating myoblasts. In differentiated muscle fibers localizes between fibers and the surrounding matrix. Isoform Alpha-7X1A and isoform Alpha-7X1B are expressed at myotendinous and neuromuscular junctions; isoform Alpha-7X1C is expressed at neuromuscular junctions and at extrasynaptic sites.1 Publication

Developmental stagei

Isoforms are developmentally regulated during the formation of skeletal muscle. Isoform Alpha-7X1A and isoform Alpha-7X1C are induced upon terminal myogenic differentiation; isoform Alpha-7X1B is present earlier in replicating cells and diminishes upon differentiation.

Gene expression databases

BgeeiENSRNOG00000007905.
ExpressionAtlasiQ63258. baseline and differential.
GenevisibleiQ63258. RN.

Interactioni

Subunit structurei

Interacts (via C-terminus intracellular tail region) with CIB1; the interaction is stabilized/increased in a calcium- and magnesium-dependent manner (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-7 associates with beta-1. Interacts with COMP.By similarity1 Publication

GO - Molecular functioni

  • cell adhesion molecule binding Source: RGD
  • integrin binding Source: RGD
  • laminin binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

CORUMiQ63258.
IntActiQ63258. 1 interactor.
STRINGi10116.ENSRNOP00000058370.

Structurei

3D structure databases

ProteinModelPortaliQ63258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 103FG-GAP 1PROSITE-ProRule annotationAdd BLAST66
Repeati110 – 165FG-GAP 2PROSITE-ProRule annotationAdd BLAST56
Repeati185 – 238FG-GAP 3PROSITE-ProRule annotationAdd BLAST54
Repeati248 – 305FG-GAP 4PROSITE-ProRule annotationAdd BLAST58
Repeati306 – 367FG-GAP 5PROSITE-ProRule annotationAdd BLAST62
Repeati368 – 423FG-GAP 6PROSITE-ProRule annotationAdd BLAST56
Repeati427 – 486FG-GAP 7PROSITE-ProRule annotationAdd BLAST60
Repeati1111 – 111414
Repeati1119 – 112224
Repeati1127 – 113034

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1111 – 11303 X 4 AA repeats of D-X-H-PAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1061 – 1065GFFKR motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi908 – 913Poly-Arg6

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPBB. Eukaryota.
ENOG410XVGZ. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ63258.
KOiK06583.
PhylomeDBiQ63258.

Family and domain databases

InterProiView protein in InterPro
IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
PfamiView protein in Pfam
PF01839. FG-GAP. 2 hits.
PF08441. Integrin_alpha2. 1 hit.
PRINTSiPR01185. INTEGRINA.
SMARTiView protein in SMART
SM00191. Int_alpha. 5 hits.
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiView protein in PROSITE
PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Alpha-7X1B (identifier: Q63258-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARIPRCDFL GLPGICYLLS FLLAGLLLPR ASAFNLDVMG AIRKEGEPGS
60 70 80 90 100
LFGFSVALHR QLQPRPQSWL LVGAPQALAL PGQQANRTGG LFACPLSLEE
110 120 130 140 150
TDCYRVDIDR GANVQKESKE NQWLGVSVRS QGAGGKVVTC AHRYESRQRV
160 170 180 190 200
DQVLETRDVI GRCFVLSQDL AIRDELDGGE WKFCEGRPQG HEQFGFCQQG
210 220 230 240 250
TAATFSPDSH YLIFGAPGTY NWKGLLFVTN IDSSDPDQLV YKTLDPADRL
260 270 280 290 300
TGPAGDLTLN SYLGFSIDSG KGLMRSEELS FVAGAPRANH KGAVVILRKD
310 320 330 340 350
SASRLIPEVV LSGERLTSGF GYSLAVTDLN SDGWADLIVG APYFFERQEE
360 370 380 390 400
LGGAVYVYMN QGGHWADISP LRLCGSPDSM FGISLAVLGD LNQDGFPDIA
410 420 430 440 450
VGAPFDGDGK VFIYHGSSLG VVTKPSQVLE GEAVGIKSFG YSLSGGLDVD
460 470 480 490 500
GNHYPDLLVG SLADTAALFR ARPVLHVSQE IFIDPRAIDL EQPNCADGRL
510 520 530 540 550
VCVHVKVCFS YVAVPSSYSP IVVLDYVLDG DTDRRLRGQA PRVTFPGRGP
560 570 580 590 600
DDLKHQSSGT VSLKHQHDRV CGDTVFQLQE NVKDKLRAIV VTLSYGLQTP
610 620 630 640 650
RLRRQAPDQG LPLVAPILNA HQPSTQRTEI HFLKQGCGDD KICQSNLQLA
660 670 680 690 700
QAQFCSRISD TEFQALPMDL DGTALFALSG QPFIGLELTV TNLPSDPARP
710 720 730 740 750
QADGDDAHEA QLLATLPASL RYSGVRTLDS VEKPLCLSNE NASHVECELG
760 770 780 790 800
NPMKRGTQVT FYLILSTSGI TIETTELKVE LLLATISEQD LHPVSVRAHV
810 820 830 840 850
FIELPLSISG VATPQQLFFS GKVKGESAMR SERDVGSKVK YEVTVSNQGQ
860 870 880 890 900
SLNTLGSAFL NIMWPHEIAN GKWLLYPMRV ELEGGQGPEK KGICSPRPNI
910 920 930 940 950
LHLDVDSRDR RRRELGQPEP QEPPEKVEPS TSWWPVSSAE KRNVTLDCAQ
960 970 980 990 1000
GTAKCVVFSC PLYSFDRAAV LHVWGRLWNS TFLEEYMSVK SLEVIVRANI
1010 1020 1030 1040 1050
TVKSSIKNLL LRDASTVIPV MVYLDPVAVV AEGVPWWVIL LAVLAGLLVL
1060 1070 1080 1090 1100
ALLVLLLWKL GFFKRAKHPE ATVPQYHAVK ILREDRQQFK EEKTGTIQRS
1110 1120 1130
NWGNSQWEGS DAHPILAADW HPELGPDGHP VSVTA
Length:1,135
Mass (Da):124,194
Last modified:July 10, 2007 - v2
Checksum:iEEBA7063537A4AF1
GO
Isoform Alpha-7X1A (identifier: Q63258-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1060-1135: LGFFKRAKHP...PDGHPVSVTA → CGFFRRNSPS...LRPLYPSTTQ

Show »
Length:1,116
Mass (Da):121,678
Checksum:iBBE7FBE7FFEF50A4
GO
Isoform Alpha-7X1C (identifier: Q63258-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1065-1135: RAKHPEATVP...PDGHPVSVTA → CAVPAQRILSIY

Show »
Length:1,076
Mass (Da):117,520
Checksum:i303F816B3547B48E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79 – 95ALPGQ…LFACP → DSYPDSRQIAHGRPLCLS in CAA46170 (PubMed:1315319).CuratedAdd BLAST17
Sequence conflicti130 – 133SQGA → PRES in CAA46170 (PubMed:1315319).Curated4
Sequence conflicti149R → E in CAA46170 (PubMed:1315319).Curated1
Sequence conflicti225 – 232LLFVTNID → TARVELCAQG in CAA46170 (PubMed:1315319).Curated8
Sequence conflicti236 – 259PDQLV…GDLTL → LAQVDDGPYEAGGEKDQDPR PSPVPA in CAA46170 (PubMed:1315319).CuratedAdd BLAST24
Sequence conflicti378D → H in CAA46170 (PubMed:1315319).Curated1
Sequence conflicti575 – 582VFQLQENV → CVPAAGKR in CAA46170 (PubMed:1315319).Curated8
Sequence conflicti616P → G in CAA46170 (PubMed:1315319).Curated1
Sequence conflicti650A → V in CAA46170 (PubMed:1315319).Curated1
Sequence conflicti678 – 679LS → HG in CAA46170 (PubMed:1315319).Curated2
Sequence conflicti833 – 835RDV → WDE in CAA46170 (PubMed:1315319).Curated3
Sequence conflicti949 – 950AQ → P in CAA46170 (PubMed:1315319).Curated2
Sequence conflicti996V → G in CAA52346 (PubMed:8126096).Curated1
Sequence conflicti996V → G in CAA52347 (PubMed:8126096).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0027341060 – 1135LGFFK…VSVTA → CGFFRRNSPSSSFPANYHRA HLAVQPSAMEAGGPGTVGWD SSSGRSTLRPLYPSTTQ in isoform Alpha-7X1A. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_0027351065 – 1135RAKHP…VSVTA → CAVPAQRILSIY in isoform Alpha-7X1C. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088846 mRNA. Translation: AAH88846.1.
X65036 mRNA. Translation: CAA46170.1.
X74293 mRNA. Translation: CAA52346.1.
X74294 mRNA. Translation: CAA52347.1.
PIRiS38783.
S40148.
S40149.
RefSeqiNP_110469.1. NM_030842.1. [Q63258-1]
UniGeneiRn.54492.

Genome annotation databases

EnsembliENSRNOT00000061657; ENSRNOP00000058370; ENSRNOG00000007905. [Q63258-1]
GeneIDi81008.
KEGGirno:81008.
UCSCiRGD:71022. rat. [Q63258-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiITA7_RAT
AccessioniPrimary (citable) accession number: Q63258
Secondary accession number(s): Q5HZX9, Q63026, Q63027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 10, 2007
Last modified: September 27, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families