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Protein

Interleukin-4 receptor subunit alpha

Gene

Il4r

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2 (By similarity).By similarity
Isoform 2 (soluble form) inhibits IL4-induced spleen cell proliferation.

GO - Molecular functioni

  • cytokine receptor activity Source: InterPro
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-4 receptor subunit alpha
Short name:
IL-4 receptor subunit alpha
Short name:
IL-4R subunit alpha
Short name:
IL-4R-alpha
Short name:
IL-4RA
Alternative name(s):
CD_antigen: CD124
Gene namesi
Name:Il4r
Synonyms:Il4ra
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2899. Il4r.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 232207ExtracellularSequence analysisAdd
BLAST
Transmembranei233 – 25624HelicalSequence analysisAdd
BLAST
Topological domaini257 – 801545CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular space Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
  • receptor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 801776Interleukin-4 receptor subunit alphaPRO_0000010892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 44By similarity
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Disulfide bondi74 ↔ 86By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Modified residuei164 – 1641PhosphoserineBy similarity
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence analysis
Modified residuei492 – 4921PhosphotyrosineBy similarity

Post-translational modificationi

On IL4 binding, phosphorylated on C-terminal tyrosine residues.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63257.
PRIDEiQ63257.

PTM databases

iPTMnetiQ63257.
PhosphoSiteiQ63257.

Expressioni

Tissue specificityi

Isoform 2 is expressed in kidney, spleen, lung and liver.

Gene expression databases

BgeeiENSRNOG00000015441.
GenevisibleiQ63257. RN.

Interactioni

Subunit structurei

The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3. Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP. Interacts with JAK1 through a Box 1-containing region; inhibited by SOCS5. Interacts with SOCS5; inhibits IL4 signaling (By similarity).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020994.

Structurei

3D structure databases

ProteinModelPortaliQ63257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 22399Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni439 – 549111Required for IRS1 activation and IL4-induced cell growthBy similarityAdd
BLAST
Regioni549 – 64496Required for IL4-induced gene expressionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi212 – 2165WSXWS motif
Motifi262 – 2709Box 1 motif
Motifi698 – 7036ITIM motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi375 – 3806Poly-Glu

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGBQ. Eukaryota.
ENOG41124QC. LUCA.
GeneTreeiENSGT00510000049182.
HOGENOMiHOG000090263.
HOVERGENiHBG052116.
InParanoidiQ63257.
KOiK05071.
OMAiSPCCGCC.
OrthoDBiEOG091G010H.
PhylomeDBiQ63257.
TreeFamiTF337996.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR003531. Hempt_rcpt_S_F1_CS.
IPR013783. Ig-like_fold.
IPR015319. IL-4_rcpt-alpha_N.
[Graphical view]
PfamiPF09238. IL4Ra_N. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 1 hit.
PS01355. HEMATOPO_REC_S_F1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63257-1) [UniParc]FASTAAdd to basket
Also known as: Membrane-bound form, mIL4R

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGWLCTKFLS SVSCLILLWV TGSGGIKVLG DPTCFSDYIR TSTCEWQLDS
60 70 80 90 100
TVDCSSQLLL DYRLLFEFSE NLTCTPKNSA DTVCVCQMAI EEPIQADTYW
110 120 130 140 150
LELWSERGQL WQGSFKPSDN VKPPAPDNLT LHTNVSNALL LMWSNPYPSN
160 170 180 190 200
NFLHKGLICM VNISREDNPA EFKVYNVTYT EPKLSFPVNT LTSGVRYRAR
210 220 230 240 250
VRVLSQSFPG IWSEWSPSIT WYNHFQLPLL QRLPLGVSIS CICILLFCLT
260 270 280 290 300
CYFSIIKIKK IWWDQIPTPA RSPLAAIIIQ DTKVSLWEKQ TRSQESTKSR
310 320 330 340 350
HWKTCLTKLL PCLLEHRVKK ERESPKAAKT KPLQSPEKAG WYPAEVSRTV
360 370 380 390 400
LWPENVHVSV VRCMELFEAP VQNVEEEEDE MVKGDLSMSP ENSGGGFQES
410 420 430 440 450
QADIMARLTE NLFSDLLGAE NGGVGQSSMA ESSSLLPSES GQASTSWACF
460 470 480 490 500
PTGPSETTCQ VTGQQPPHPD PERATGTACT QVPLVISDNP AYRSFSDFSS
510 520 530 540 550
PAPNPGELAS EQKQAGHLEE GDLLSPVDPH SSGPPMQQAE SWEQILHMSV
560 570 580 590 600
LQHGTAGSTP APTSGYQEFV QAVKQGASQD AGVPGVGPSG DTGYKAFSSL
610 620 630 640 650
LSSSGVCTDT AAAGTDSGCG GYKPFQNPVP NQSPNSMPLF TFGLDMELPP
660 670 680 690 700
SPLNSAPPNS TPECLGLELG LKGGTWLKAP PPSEQVPKPF GDDLGLGIVY
710 720 730 740 750
SSLTCHLCGH LKQHHSQEED GQIHVVASPG CGCCYDEKSP SLGNLSGTLE
760 770 780 790 800
SCPGEMSQEA NLTLAPRTPS NLSGVGKGPG HSPVPSQTTE VPVGTLGVTV

S
Length:801
Mass (Da):86,719
Last modified:July 19, 2004 - v2
Checksum:i835E98D5E800EC76
GO
Isoform 2 (identifier: Q63257-2) [UniParc]FASTAAdd to basket
Also known as: Soluble form, sIL4R

The sequence of this isoform differs from the canonical sequence as follows:
     224-229: HFQLPL → PNNVNL
     230-801: Missing.

Show »
Length:229
Mass (Da):26,001
Checksum:iF3C6DE348B1A162F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31W → R in CAA49528 (PubMed:7640343).Curated
Sequence conflicti138 – 1381A → G in CAA49528 (PubMed:7640343).Curated
Sequence conflicti285 – 2851S → L in CAA49528 (PubMed:7640343).Curated
Sequence conflicti580 – 5823DAG → ARW in CAA49528 (PubMed:7640343).Curated
Sequence conflicti728 – 7281S → C in CAA49528 (PubMed:7640343).Curated
Sequence conflicti776 – 7772GK → Q in CAA49528 (PubMed:7640343).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei224 – 2296HFQLPL → PNNVNL in isoform 2. 1 PublicationVSP_011118
Alternative sequencei230 – 801572Missing in isoform 2. 1 PublicationVSP_011119Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69903 mRNA. Translation: CAA49528.1.
AB015746 mRNA. Translation: BAA78337.1.
AB015747 mRNA. Translation: BAA78338.1.
PIRiS31575.
RefSeqiNP_596871.2. NM_133380.2. [Q63257-1]
XP_006230277.1. XM_006230215.2. [Q63257-1]
XP_006230278.1. XM_006230216.2. [Q63257-1]
XP_008758084.1. XM_008759862.1. [Q63257-1]
XP_008758085.1. XM_008759863.1. [Q63257-1]
XP_008758086.1. XM_008759864.1. [Q63257-1]
UniGeneiRn.10471.

Genome annotation databases

EnsembliENSRNOT00000020994; ENSRNOP00000020994; ENSRNOG00000015441. [Q63257-1]
ENSRNOT00000088073; ENSRNOP00000073767; ENSRNOG00000015441. [Q63257-2]
GeneIDi25084.
KEGGirno:25084.
UCSCiRGD:2899. rat. [Q63257-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69903 mRNA. Translation: CAA49528.1.
AB015746 mRNA. Translation: BAA78337.1.
AB015747 mRNA. Translation: BAA78338.1.
PIRiS31575.
RefSeqiNP_596871.2. NM_133380.2. [Q63257-1]
XP_006230277.1. XM_006230215.2. [Q63257-1]
XP_006230278.1. XM_006230216.2. [Q63257-1]
XP_008758084.1. XM_008759862.1. [Q63257-1]
XP_008758085.1. XM_008759863.1. [Q63257-1]
XP_008758086.1. XM_008759864.1. [Q63257-1]
UniGeneiRn.10471.

3D structure databases

ProteinModelPortaliQ63257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020994.

PTM databases

iPTMnetiQ63257.
PhosphoSiteiQ63257.

Proteomic databases

PaxDbiQ63257.
PRIDEiQ63257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020994; ENSRNOP00000020994; ENSRNOG00000015441. [Q63257-1]
ENSRNOT00000088073; ENSRNOP00000073767; ENSRNOG00000015441. [Q63257-2]
GeneIDi25084.
KEGGirno:25084.
UCSCiRGD:2899. rat. [Q63257-1]

Organism-specific databases

CTDi3566.
RGDi2899. Il4r.

Phylogenomic databases

eggNOGiENOG410IGBQ. Eukaryota.
ENOG41124QC. LUCA.
GeneTreeiENSGT00510000049182.
HOGENOMiHOG000090263.
HOVERGENiHBG052116.
InParanoidiQ63257.
KOiK05071.
OMAiSPCCGCC.
OrthoDBiEOG091G010H.
PhylomeDBiQ63257.
TreeFamiTF337996.

Miscellaneous databases

PROiQ63257.

Gene expression databases

BgeeiENSRNOG00000015441.
GenevisibleiQ63257. RN.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR003531. Hempt_rcpt_S_F1_CS.
IPR013783. Ig-like_fold.
IPR015319. IL-4_rcpt-alpha_N.
[Graphical view]
PfamiPF09238. IL4Ra_N. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 1 hit.
PS01355. HEMATOPO_REC_S_F1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIL4RA_RAT
AccessioniPrimary (citable) accession number: Q63257
Secondary accession number(s): Q9R1W8, Q9WTM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.