Q63245 (FOXD3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Forkhead box protein D3

Alternative name(s):
HNF3/FH transcription factor genesis
Hepatocyte nuclear factor 3 forkhead homolog 2
Short name=HFH-2

Gene names

Name:	Foxd3
Synonyms:	Hfh2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	101 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Developmental protein Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon extension involved in axon guidance Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome cartilage development Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic placenta development Inferred from Biological aspect of Ancestor. Source: RefGenome enteric nervous system development Inferred from Biological aspect of Ancestor. Source: RefGenome iridophore differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome kidney development Inferred from Biological aspect of Ancestor. Source: RefGenome lateral line nerve glial cell development Inferred from Biological aspect of Ancestor. Source: RefGenome melanocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB neural crest cell migration Inferred from Biological aspect of Ancestor. Source: RefGenome pattern specification process Inferred from Biological aspect of Ancestor. Source: RefGenome peripheral nervous system development Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome sympathetic nervous system development Inferred from Biological aspect of Ancestor. Source: RefGenome trophectodermal cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	DNA binding, bending Inferred from Biological aspect of Ancestor. Source: RefGenome RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome double-stranded DNA binding Inferred from mutant phenotype Ref.1. Source: RGD sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from Biological aspect of Ancestor. Source: RefGenome transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – ›101

›101

Forkhead box protein D3

PRO_0000091819

Regions

DNA binding

3 – 97

95

Fork-head

Experimental info

Non-terminal residue

1

1

Non-terminal residue

101

1

Secondary structure

1

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101

Helix Strand Turn

Details...

Helix

8 – 17

10

Turn

20 – 22

3

Helix

26 – 36

11

Helix

38 – 43

6

Helix

47 – 59

13

Beta strand

62 – 64

3

Beta strand

71 – 73

3

Beta strand

76 – 78

3

Helix

83 – 89

7

Beta strand

94 – 96

3

Sequences

	Sequence		Length	Mass (Da)
	Q63245 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DC0ADEC15A1DD143	FASTA	101	11,943
	10 20 30 40 50 60 LVKPPYSYIA LITMAILQSP QKKLTLSGIC EFISNRFPYY REKFPAWQNS IRHNLSLNDC 70 80 90 100 FVKIPREPGN PGKGNYWTLD PQSEDMFDNG SFLRRRKRFK R

References

[1]	"Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family." Clevidence D.E., Overdier D.G., Tao W., Qian X., Pani L., Lai E., Costa R.H. Proc. Natl. Acad. Sci. U.S.A. 90:3948-3952(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins." Marsden I., Jin C., Liao X. J. Mol. Biol. 278:293-299(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 3-97.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L13202 mRNA. Translation: AAA41319.1.

IPI

IPI00765696.

PIR

I60917.

UniGene

Rn.92964.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HDC	NMR	-	A	2-98	[»]
2HFH	NMR	-	A	2-101	[»]

ProteinModelPortal

Q63245.

SMR

Q63245. Positions 2-98.

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000054694.

PTM databases

PhosphoSite

Q63245.

Proteomic databases

PaxDb

Q63245.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

UCSC

RGD:621715. rat.

Organism-specific databases

RGD

621715. Foxd3.

Phylogenomic databases

eggNOG

COG5025.

HOGENOM

HOG000231285.

HOVERGEN

HBG083674.

InParanoid

Q63245.

Gene expression databases

ArrayExpress

Q63245.

Genevestigator

Q63245.

GermOnline

ENSRNOG00000009021. Rattus norvegicus.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00250. Fork_head. 1 hit.
[Graphical view]

PRINTS

PR00053. FORKHEAD.

SMART

SM00339. FH. 1 hit.
[Graphical view]

PROSITE

PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q63245.

Entry information

Entry name

FOXD3_RAT

Accession

Primary (citable) accession number: Q63245

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program