Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein subunit alpha-12

Gene

Gna12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. May play a role in the control of cell migration through the TOR signaling cascade.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691MagnesiumBy similarity
Metal bindingi206 – 2061MagnesiumBy similarity
Binding sitei351 – 3511GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 698GTPBy similarity
Nucleotide bindingi200 – 2067GTPBy similarity
Nucleotide bindingi225 – 2295GTPBy similarity
Nucleotide bindingi294 – 2974GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-12
Short name:
G alpha-12
Short name:
G-protein subunit alpha-12
Gene namesi
Name:Gna12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi71018. Gna12.

Subcellular locationi

  • Membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • brush border membrane Source: RGD
  • focal adhesion Source: Ensembl
  • heterotrimeric G-protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi229 – 2291Q → L: Constitutively active. Interacts with PPP5C, activates its phosphatase activity and translocates PPP5C to the plasma membrane. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Guanine nucleotide-binding protein subunit alpha-12PRO_0000203772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi11 – 111S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ63210.
PRIDEiQ63210.

Expressioni

Gene expression databases

ExpressionAtlasiQ63210. baseline and differential.
GenevisibleiQ63210. RN.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22 (By similarity).By similarity

GO - Molecular functioni

  • D5 dopamine receptor binding Source: RGD

Protein-protein interaction databases

MINTiMINT-132109.
STRINGi10116.ENSRNOP00000001654.

Structurei

3D structure databases

ProteinModelPortaliQ63210.
SMRiQ63210. Positions 54-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(12) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ63210.
KOiK04346.
OMAiGISEHIF.
OrthoDBiEOG7HF1JF.
PhylomeDBiQ63210.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000469. Gprotein_alpha_12/13.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q63210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR
60 70 80 90 100
AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL
110 120 130 140 150
KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP
160 170 180 190 200
ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL
210 220 230 240 250
LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV
260 270 280 290 300
SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV
310 320 330 340 350
EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR GKPLFHHFTT
360 370
AIDTENIRFV FHAVKDTILQ ENLKDIMLQ
Length:379
Mass (Da):44,065
Last modified:January 23, 2007 - v3
Checksum:iD178524AD7A0A9FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85760 mRNA. Translation: BAA12867.1.
RefSeqiNP_112296.1. NM_031034.2.
UniGeneiRn.10497.

Genome annotation databases

EnsembliENSRNOT00000001654; ENSRNOP00000001654; ENSRNOG00000001235.
GeneIDi81663.
KEGGirno:81663.
UCSCiRGD:71018. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85760 mRNA. Translation: BAA12867.1.
RefSeqiNP_112296.1. NM_031034.2.
UniGeneiRn.10497.

3D structure databases

ProteinModelPortaliQ63210.
SMRiQ63210. Positions 54-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-132109.
STRINGi10116.ENSRNOP00000001654.

Proteomic databases

PaxDbiQ63210.
PRIDEiQ63210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001654; ENSRNOP00000001654; ENSRNOG00000001235.
GeneIDi81663.
KEGGirno:81663.
UCSCiRGD:71018. rat.

Organism-specific databases

CTDi2768.
RGDi71018. Gna12.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ63210.
KOiK04346.
OMAiGISEHIF.
OrthoDBiEOG7HF1JF.
PhylomeDBiQ63210.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiR-RNO-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiQ63210.

Gene expression databases

ExpressionAtlasiQ63210. baseline and differential.
GenevisibleiQ63210. RN.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000469. Gprotein_alpha_12/13.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of rat G alpha 12."
    Mitsui H., Exton J.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Galpha(12) and Galpha(13) interact with Ser/Thr protein phosphatase type 5 and stimulate its phosphatase activity."
    Yamaguchi Y., Katoh H., Mori K., Negishi M.
    Curr. Biol. 12:1353-1358(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPP5C, SUBCELLULAR LOCATION, MUTAGENESIS OF GLN-229.

Entry informationi

Entry nameiGNA12_RAT
AccessioniPrimary (citable) accession number: Q63210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.