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Protein

Guanine nucleotide-binding protein subunit alpha-12

Gene

Gna12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:12176367, PubMed:21212405). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (By similarity). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (PubMed:21212405). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (By similarity). Together with NAPA promotes CDH5 localization to plasma membrane (By similarity). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:12176367).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69MagnesiumBy similarity1
Metal bindingi206MagnesiumBy similarity1
Binding sitei351GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 70GTPBy similarity6
Nucleotide bindingi200 – 203GTPBy similarity4
Nucleotide bindingi294 – 297GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-416482 G alpha (12/13) signalling events
R-RNO-456926 Thrombin signalling through proteinase activated receptors (PARs)

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-12
Short name:
G alpha-12
Short name:
G-protein subunit alpha-12
Gene namesi
Name:Gna12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi71018 Gna12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi229Q → L: Constitutively active. Interacts with PPP5C, activates its phosphatase activity and translocates PPP5C to the plasma membrane. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002037721 – 379Guanine nucleotide-binding protein subunit alpha-12Add BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi11S-palmitoyl cysteineBy similarity1
Modified residuei206PhosphothreonineBy similarity1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ63210
PRIDEiQ63210

Expressioni

Gene expression databases

BgeeiENSRNOG00000001235
ExpressionAtlasiQ63210 baseline and differential
GenevisibleiQ63210 RN

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane. Interacts with RGS22. Interacts (via N-terminus) with NAPA; the interaction promotes CDH5 localization to plasma membrane. Interacts with CTNND1 (via N-terminus); the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with PPP2R1A; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts (in GTP-bound form) with ARHGEF1. Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain). Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001654

Structurei

3D structure databases

ProteinModelPortaliQ63210
SMRiQ63210
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(12) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038729
HOVERGENiHBG063184
InParanoidiQ63210
KOiK04346
OMAiKEYQHVI
OrthoDBiEOG091G0NV2
PhylomeDBiQ63210
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR000469 Gprotein_alpha_12/13
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00440 GPROTEINA12
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

Q63210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR
60 70 80 90 100
AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL
110 120 130 140 150
KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP
160 170 180 190 200
ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL
210 220 230 240 250
LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV
260 270 280 290 300
SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV
310 320 330 340 350
EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR GKPLFHHFTT
360 370
AIDTENIRFV FHAVKDTILQ ENLKDIMLQ
Length:379
Mass (Da):44,065
Last modified:January 23, 2007 - v3
Checksum:iD178524AD7A0A9FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85760 mRNA Translation: BAA12867.1
RefSeqiNP_112296.1, NM_031034.2
UniGeneiRn.10497

Genome annotation databases

EnsembliENSRNOT00000001654; ENSRNOP00000001654; ENSRNOG00000001235
GeneIDi81663
KEGGirno:81663
UCSCiRGD:71018 rat

Entry informationi

Entry nameiGNA12_RAT
AccessioniPrimary (citable) accession number: Q63210
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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