Q631K2 (CLPP2_BACCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	BCE33L4844

Organism

Bacillus cereus (strain ZK / E33L) [Complete proteome] [HAMAP]

Taxonomic identifier

288681 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000179491
Sites
	Active site	98	1	By similarity
	Active site	123	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q631K2 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: AB05E7B3E532B620	FASTA	193	21,420
	10 20 30 40 50 60 MNLIPTVIEQ TNRGERAYDI YSRLLKDRII MLGSAIDDNV ANSIVSQLLF LESQDPEKDI 70 80 90 100 110 120 HIYINSPGGS ITAGMAIYDT MQFIKPQVST ICIGMAASMG AFLLAAGEKG KRYALPNSEV 130 140 150 160 170 180 MIHQPLGGAQ GQATEIEIAA KRILFLREKL NQILADRTGQ PLEVLQRDTD RDNFMTAEKA 190 LEYGLIDKIF TNR

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ZK / E33L.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000001 Genomic DNA. Translation: AAU15433.1.
RefSeq	YP_086415.1. NC_006274.1.
3D structure databases
ProteinModelPortal	Q631K2.
SMR	Q631K2. Positions 3-193.
ModBase	Search...
Protein-protein interaction databases
STRING	Q631K2.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000044561; EBBACP00000043462; EBBACG00000044552.
GeneID	3022633.
GenomeReviews	Gene locus BCE33L4844 in contig CP000001_GR.
KEGG	bcz:BCZK4844.
PATRIC	18893634. VBIBacCer95304_5125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
GeneTree	EBGT00050000002046.
HOGENOM	HBG558421.
OMA	MEAQDFG.
PhylomeDB	Q631K2.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	BCER288681:BCE33L4844-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_BACCZ

Accession

Primary (citable) accession number: Q631K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program