Q63199 (TNR6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor receptor superfamily member 6

Alternative name(s):
Apo-1 antigen
Apoptosis-mediating surface antigen FAS
FASLG receptor
CD_antigen=CD95

Gene names

Name:	Fas
Synonyms:	Apt1, Tnfrsf6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both By similarity.
Subunit structure	Binds DAXX and RIPK1. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Interacts with BRE and FEM1B By similarity. Interacts with FADD By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Domain	Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Sequence similarities	Contains 1 death domain. Contains 3 TNFR-Cys repeats.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 16936193. Source: RGD cellular response to organic cyclic compound Inferred from expression pattern PubMed 16987298. Source: RGD dendrite regeneration Inferred from mutant phenotype PubMed 18692025. Source: RGD extrinsic apoptotic signaling pathway via death domain receptors Inferred from expression pattern PubMed 17195944. Source: RGD immune response Inferred from electronic annotation. Source: InterPro induction of apoptosis Inferred from expression pattern PubMed 15528299. Source: RGD ovulation cycle Inferred from expression pattern PubMed 16461545. Source: RGD regulation of cell proliferation Inferred from mutant phenotype PubMed 17991709. Source: RGD response to drug Inferred from expression pattern PubMed 16687611. Source: RGD response to estrogen stimulus Inferred from expression pattern PubMed 16099864. Source: RGD response to growth factor stimulus Inferred from expression pattern PubMed 18692025. Source: RGD response to hypoxia Inferred from expression pattern PubMed 16870148. Source: RGD response to inorganic substance Inferred from expression pattern PubMed 17953211. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 17899301. Source: RGD response to peptide hormone stimulus Inferred from expression pattern PubMed 16392621. Source: RGD response to toxin Inferred from expression pattern PubMed 17900647. Source: RGD spermatogenesis Inferred from expression pattern PubMed 17195944. Source: RGD
Cellular_component	CD95 death-inducing signaling complex Inferred from direct assay PubMed 16493077. Source: RGD cell surface Inferred from direct assay PubMed 18045865. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay PubMed 17518537. Source: RGD perinuclear region of cytoplasm Inferred from direct assay PubMed 17518537. Source: RGD
Molecular_function	tumor necrosis factor-activated receptor activity Inferred from mutant phenotype PubMed 16154149. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 324	303	Tumor necrosis factor receptor superfamily member 6		PRO_0000034569
Regions
	Topological domain	22 – 171	150	Extracellular Potential
	Transmembrane	172 – 188	17	Helical; Potential
	Topological domain	189 – 324	136	Cytoplasmic Potential
	Repeat	43 – 79	37	TNFR-Cys 1
	Repeat	80 – 123	44	TNFR-Cys 2
	Repeat	124 – 163	40	TNFR-Cys 3
	Domain	219 – 303	85	Death
	Region	201 – 306	106	Interaction with HIPK3 By similarity
Amino acid modifications
	Modified residue	214	1	Phosphoserine By similarity
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 55		By similarity
	Disulfide bond	56 ↔ 69		By similarity
	Disulfide bond	59 ↔ 78		By similarity
	Disulfide bond	81 ↔ 97		By similarity
	Disulfide bond	100 ↔ 115		By similarity
	Disulfide bond	103 ↔ 123		By similarity
	Disulfide bond	125 ↔ 139		By similarity
	Disulfide bond	142 ↔ 154		By similarity
	Disulfide bond	145 ↔ 162		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63199 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D25D583C909D9D09	FASTA	324	36,835
	10 20 30 40 50 60 MLWIMAVLPL VLAGPELNVR MQGTDSIFEG LELKRSVRET DNNCSEGLYQ VGPFCCQPCQ 70 80 90 100 110 120 PGERKVKDCT TSGGAPTCHP CTEGEEYTDR KHYSDKCRRC AFCDEGHGLE VETNCTRTQN 130 140 150 160 170 180 TKCRCKENFY CNASLCDHCY HCTSCGLEDI LEPCTRTSNT KCKKQSSNYK LLWLLILPGL 190 200 210 220 230 240 AILFVFIYKR YRKRQPGDPE SGIPSPESVP MNVSDVNLNK YIWRTAEKMK ICDAKKFARQ 250 260 270 280 290 300 HKIPESKIDE IEHNSPQDAA EQKIQLLQCW YQSHGKTGAC QALIQGLRKA NRCDIAEEIQ 310 320 AMVWEDHENS ISNSRNENEG QSLE

References

[1]	"A variant mRNA species encoding a truncated form of Fas antigen in the rat liver." Kimura K., Yamamoto M., Wakatsuki T. Biochem. Biophys. Res. Commun. 198:666-674(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26112 mRNA. Translation: BAA05108.1.
IPI	IPI00206056.
PIR	JC2395.
RefSeq	NP_631933.2. NM_139194.2.
UniGene	Rn.162521.
3D structure databases
ProteinModelPortal	Q63199.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q63199. 1 interaction.
STRING	10116.ENSRNOP00000040348.
Proteomic databases
PRIDE	Q63199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	246097.
KEGG	rno:246097.
UCSC	RGD:619831. rat.
Organism-specific databases
CTD	355.
RGD	619831. Fas.
Phylogenomic databases
eggNOG	NOG45364.
HOGENOM	HOG000139681.
HOVERGEN	HBG004091.
KO	K04390.
OrthoDB	EOG4K6G53.
Gene expression databases
ArrayExpress	Q63199.
Genevestigator	Q63199.
GermOnline	ENSRNOG00000019142. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR011029. DEATH-like_dom. IPR000488. Death_domain. IPR008063. Fas_rcpt. IPR001368. TNFR/NGFR_Cys_rich_reg. [Graphical view]
Pfam	PF00531. Death. 1 hit. PF00020. TNFR_c6. 1 hit. [Graphical view]
PRINTS	PR01680. TNFACTORR6.
SMART	SM00005. DEATH. 1 hit. SM00208. TNFR. 3 hits. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50017. DEATH_DOMAIN. 1 hit. PS00652. TNFR_NGFR_1. 2 hits. PS50050. TNFR_NGFR_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	623366.

Entry information

Entry name

TNR6_RAT

Accession

Primary (citable) accession number: Q63199

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program