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Protein

Transcription elongation factor B polypeptide 3

Gene

Tceb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).2 Publications

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD

GO - Biological processi

  • regulation of transcription from RNA polymerase II promoter Source: RGD
  • transcription elongation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor B polypeptide 3
Alternative name(s):
Elongin 110 kDa subunit
Elongin-A
Short name:
EloA
RNA polymerase II transcription factor SIII subunit A1
SIII p110
Gene namesi
Name:Tceb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3827. Tceb3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • elongin complex Source: GO_Central
  • integral component of membrane Source: InterPro
  • nucleus Source: RGD
  • transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi549 – 5491T → I: Reduces transcription activity. 1 Publication
Mutagenesisi550 – 5501L → S: Reduces transcription activity and abolishes heterotrimeric formation. 1 Publication
Mutagenesisi554 – 5541C → F: Reduces transcription activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773Transcription elongation factor B polypeptide 3PRO_0000086962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951PhosphoserineCombined sources
Modified residuei310 – 3101PhosphoserineBy similarity
Modified residuei380 – 3801PhosphoserineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity
Modified residuei430 – 4301N6-acetyllysineBy similarity
Modified residuei515 – 5151PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63187.
PRIDEiQ63187.

PTM databases

iPTMnetiQ63187.
PhosphoSiteiQ63187.

Interactioni

Subunit structurei

Heterotrimer of an A (A1, A2 or A3), B and C subunit.1 Publication

Protein-protein interaction databases

IntActiQ63187. 5 interactions.
MINTiMINT-4598437.
STRINGi10116.ENSRNOP00000059109.

Structurei

3D structure databases

ProteinModelPortaliQ63187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 7976TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini565 – 60945F-boxPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni521 – 680160Activation domainAdd
BLAST
Regioni549 – 55810Interacting with Elongin BC complex

Domaini

The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2821. Eukaryota.
ENOG4110PG3. LUCA.
HOGENOMiHOG000059565.
HOVERGENiHBG051467.
InParanoidiQ63187.
PhylomeDBiQ63187.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR010684. RNA_pol_II_trans_fac_SIII_A.
IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF06881. Elongin_A. 1 hit.
PF08711. Med26. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS51319. TFIIS_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAESALQVV EKLQARLAAN PDPKKLLKYL KKLSVLPITV DILVETGVGK
60 70 80 90 100
TVNSFRKHEQ VGNFARDLVA QWKKLVPVER NNEAEDQDFE KSNSRKRPRD
110 120 130 140 150
VPQQEEEAEG NYQESWQASG SQPYSPEHRQ KKHRKLPELE RPHKVAHGHE
160 170 180 190 200
RRDERKRCHK VSPPYSSDPE SSDYGHVQSP PPSSPHQMYT DLSRSPEMDQ
210 220 230 240 250
EPIVSHPKPG KVHSNTFQDR LGVSHLGEHQ GKGAVSQNKP HKSSHKEKRP
260 270 280 290 300
VDARGDEKSS VMGREKSHKA SSKEESRRLL SEDSAKEKLP SSVVKKEKDR
310 320 330 340 350
EGNSLKKKLS PALDVASDNH FKKPKHKDSE KIKSDKNKQS VDSVDSGRGT
360 370 380 390 400
GDPLPRAKDK VPNNLKAQEG KVRTNSDRKS PGSLPKVEEM DMDDEFEQPT
410 420 430 440 450
MSFESYLSYD QPRKKKKKVV KTSGTALGEK GLKKKDSKST SKNLNSAQKL
460 470 480 490 500
PKANENKSDK LQPAGAEPTR PRKVPTDVLP ALPDIPLPAI QTNYRPLPSL
510 520 530 540 550
ELISSFQPKR KAFSSPQEEE EAGFTGRRMN SKMQVYSGSK CAYLPKMMTL
560 570 580 590 600
HQQCIRVLKN NIDSIFEVGG VPYSVLEPVL ERCTPDQLYR IEECNHVLIE
610 620 630 640 650
ETDQLWKVHC HRDFKEERPE EYESWREMYL RLQDAREQRL RLLTNNIRSA
660 670 680 690 700
HANKPKGRQA KMAFVNSVAK PPRDVRRRQE KFGTGGAAVP EKVRIKPAPY
710 720 730 740 750
TTGSSHVPAS NSSSSFHSSP EELAYEGPST SSAHLAPVAS SSVSYDPRKP
760 770
AVKKIAPMMA KTIKAFKNRF SRR
Length:773
Mass (Da):87,203
Last modified:November 1, 1996 - v1
Checksum:iAEEE76E6953165AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46816 mRNA. Translation: AAA82095.1.
PIRiA57244.
UniGeneiRn.37427.

Genome annotation databases

UCSCiRGD:3827. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46816 mRNA. Translation: AAA82095.1.
PIRiA57244.
UniGeneiRn.37427.

3D structure databases

ProteinModelPortaliQ63187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63187. 5 interactions.
MINTiMINT-4598437.
STRINGi10116.ENSRNOP00000059109.

PTM databases

iPTMnetiQ63187.
PhosphoSiteiQ63187.

Proteomic databases

PaxDbiQ63187.
PRIDEiQ63187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3827. rat.

Organism-specific databases

RGDi3827. Tceb3.

Phylogenomic databases

eggNOGiKOG2821. Eukaryota.
ENOG4110PG3. LUCA.
HOGENOMiHOG000059565.
HOVERGENiHBG051467.
InParanoidiQ63187.
PhylomeDBiQ63187.

Miscellaneous databases

PROiQ63187.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR001810. F-box_dom.
IPR010684. RNA_pol_II_trans_fac_SIII_A.
IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF06881. Elongin_A. 1 hit.
PF08711. Med26. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS51319. TFIIS_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II."
    Aso T., Lane W.S., Conaway J.W., Conaway R.C.
    Science 269:1439-1443(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "RNA polymerase II transcription factor SIII. I. Identification, purification, and properties."
    Bradsher J.N., Jackson K.W., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 268:25587-25593(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "The inducible elongin A elongation activation domain: structure, function and interaction with the elongin BC complex."
    Aso T., Haque D., Barstead R.J., Conaway R.C., Conaway J.W.
    EMBO J. 15:5557-5566(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ACTIVATION DOMAIN, INTERACTION WITH ELONGIN BC COMPLEX, MUTAGENESIS OF THR-549; LEU-550 AND CYS-554.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiELOA1_RAT
AccessioniPrimary (citable) accession number: Q63187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.