Reviewed,
UniProtKB/Swiss-Prot Q63151 (ACSL3_RAT)
Last modified
January 19, 2010.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Long-chain-fatty-acid--CoA ligase 3 EC=6.2.1.3 Alternative name(s): Long-chain acyl-CoA synthetase 3 Short name=LACS 3 Brain acyl-CoA synthetase II | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 720 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 is required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) By similarity. Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity. Ref.3 |
| Catalytic activity | ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA. |
| Cofactor | Magnesium By similarity. |
| Subcellular location | Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity. |
| Tissue specificity | Predominantly expressed in the brain, and to a much lesser extent, in lung, adrenal gland, kidney, small intestine, and adipose tissue but not detected in heart or liver. |
| Developmental stage | Detected 5 days after birth, increased to a maximal level at 15 days, and then decreased gradually to 10% of its maximum level in adult. |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative initiation. [Align] [Select] | ||||||
| Isoform Long (identifier: Q63151-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q63151-2) The sequence of this isoform differs from the canonical sequence as follows: 1-11: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 720 | 720 | Long-chain-fatty-acid--CoA ligase 3 | PRO_0000001313 | |||||
Regions | |||||||||
| Transmembrane | 21 – 41 | 21 | Signal-anchor for type III membrane protein Potential | ||||||
| Topological domain | 42 – 720 | 679 | Cytoplasmic Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 593 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 683 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 688 | 1 | Phosphothreonine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 11 | 11 | Missing in isoform Short. | VSP_018649 | |||||
Sequences
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References
| [1] | "Molecular characterization and expression of rat acyl-CoA synthetase 3." Fujino T., Kang M.-J., Suzuki H., Iijima H., Yamamoto T.T. J. Biol. Chem. 271:16748-16752(1996) [PubMed: 8663269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: Wistar. Tissue: Brain. |
| [2] | "Alternative translation initiation generates acyl-CoA synthetase 3 isoforms with heterogeneous amino termini." Fujino T., Kang M.-J., Minekura H., Suzuki H., Yamamoto T.T. J. Biochem. 122:212-216(1997) [PubMed: 9276691] [Abstract] Cited for: ALTERNATIVE INITIATION. |
| [3] | "Suppression of long chain acyl-CoA synthetase 3 (ACSL3) decreases hepatic de novo fatty acid synthesis through decreased transcriptional activity." Bu S.Y., Mashek M.T., Mashek D.G. J. Biol. Chem. 284:30474-30483(2009) [PubMed: 19737935] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D30666 mRNA. Translation: BAA06340.1. |
| IPI | IPI00205908. IPI00760128. |
| RefSeq | NP_476448.1. |
| UniGene | Rn.54820 |
3D structure databases | |
| SMR | Q63151. Positions 132-711. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q63151. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000020161; ENSRNOP00000020161; ENSRNOG00000014718; Rattus norvegicus. [Genome view] |
| GeneID | 114024. |
| KEGG | rno:114024. |
| NMPDR | fig|10116.3.peg.30313. |
| UCSC | NM_057107. rat. |
Organism-specific databases | |
| CTD | 114024. |
| RGD | 70552. Acsl3. |
Phylogenomic databases | |
| eggNOG | roNOG09303. |
| HOVERGEN | Q63151. |
| InParanoid | Q63151. |
| OMA | NQIKAKP. |
| OrthoDB | EOG9Z0DRH. |
| PhylomeDB | Q63151. |
Enzyme and pathway databases | |
| BRENDA | 6.2.1.3. 248. |
Gene expression databases | |
| ArrayExpress | Q63151. |
| Genevestigator | Q63151. |
| GermOnline | ENSRNOG00000014718. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view] |
| Pfam | PF00501. AMP-binding. 1 hit. [Graphical view] |
| PROSITE | PS00455. AMP_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 618179. |
Entry information
| Entry name | ACSL3_RAT | ||||||||
| Accession | Primary (citable) accession number: Q63151 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
