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Q63151

- ACSL3_RAT

UniProt

Q63151 - ACSL3_RAT

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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene

Acsl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 is required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity.By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Magnesium.By similarity

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: RGD
  2. ATP binding Source: UniProtKB-KW
  3. long-chain fatty acid-CoA ligase activity Source: RGD

GO - Biological processi

  1. brain development Source: RGD
  2. fatty acid biosynthetic process Source: RGD
  3. fatty acid metabolic process Source: RGD
  4. lipid metabolic process Source: RGD
  5. long-chain fatty acid import Source: Ensembl
  6. long-chain fatty acid metabolic process Source: GOC
  7. metabolic process Source: RGD
  8. positive regulation of Golgi to plasma membrane protein transport Source: Ensembl
  9. positive regulation of phosphatidylcholine biosynthetic process Source: Ensembl
  10. positive regulation of secretion Source: Ensembl
  11. response to nutrient Source: RGD
  12. response to organic cyclic compound Source: RGD
  13. very-low-density lipoprotein particle assembly Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Brain acyl-CoA synthetase II
Long-chain acyl-CoA synthetase 3
Short name:
LACS 3
Gene namesi
Name:Acsl3
Synonyms:Acs3, Facl3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi70552. Acsl3.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. lipid particle Source: Ensembl
  5. mitochondrial outer membrane Source: UniProtKB-KW
  6. perinuclear region of cytoplasm Source: Ensembl
  7. peroxisome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Long-chain-fatty-acid--CoA ligase 3PRO_0000001313Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei683 – 6831PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63151.
PRIDEiQ63151.

Expressioni

Tissue specificityi

Predominantly expressed in the brain, and to a much lesser extent, in lung, adrenal gland, kidney, small intestine, and adipose tissue but not detected in heart or liver.

Developmental stagei

Detected 5 days after birth, increased to a maximal level at 15 days, and then decreased gradually to 10% of its maximum level in adult.

Gene expression databases

GenevestigatoriQ63151.

Interactioni

Protein-protein interaction databases

IntActiQ63151. 1 interaction.
STRINGi10116.ENSRNOP00000020161.

Structurei

3D structure databases

ProteinModelPortaliQ63151.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini42 – 720679CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiQ63151.
KOiK01897.
OMAiCDRFIFR.
OrthoDBiEOG7P2XRD.
PhylomeDBiQ63151.
TreeFamiTF314012.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Long (identifier: Q63151-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNNHVSSTPS TMKLKQTIHP ILLYFIHFII SLYTILTYIP FYFLCESKQE
60 70 80 90 100
KPNHIKAKPV SSKPDSAYRS VNSMDGLASV LYPGCDTLDK VFMYAKNKFK
110 120 130 140 150
DKRLLGTREI LNEEDEIQPN GKVFKKVILG HYNWLSYEDV FIRALDFGNG
160 170 180 190 200
LQMLGQKPKA NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
GLNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
VIVHTMAAVQ ALGVKADVDK KAHSKPLPSD IAVIMYTSGS TGIPKGVMIS
310 320 330 340 350
HSNIIASITG MARRIPRLGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSVLKPTLM AAVPEIMDRI YKNVMNKVNE
410 420 430 440 450
MSAFQRNLFI LAYNYKMEQI SKGCSTPLCD RFVFRNVRRL LGGNIRVLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES TGAGTITEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQNVTMGYY KNEAKTKADF
560 570 580 590 600
FEDENGQRWL CTGDIGEFDP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLIDNI CAYANSYHSY VIGFVVPNQK ELTELARTKG FNGTWEELCN
660 670 680 690 700
SSEMENEVLK VLSEAAISAS LEKFEIPLKI RLSPDPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK
Length:720
Mass (Da):80,458
Last modified:November 1, 1996 - v1
Checksum:i4D79FAEF25ADA527
GO
Isoform Short (identifier: Q63151-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Show »
Length:709
Mass (Da):79,302
Checksum:iFD6262C2D2794D6A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1111Missing in isoform Short. CuratedVSP_018649Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30666 mRNA. Translation: BAA06340.1.
RefSeqiNP_476448.1. NM_057107.1. [Q63151-1]
UniGeneiRn.54820.

Genome annotation databases

EnsembliENSRNOT00000020161; ENSRNOP00000020161; ENSRNOG00000014718. [Q63151-1]
GeneIDi114024.
KEGGirno:114024.
UCSCiRGD:70552. rat. [Q63151-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D30666 mRNA. Translation: BAA06340.1 .
RefSeqi NP_476448.1. NM_057107.1. [Q63151-1 ]
UniGenei Rn.54820.

3D structure databases

ProteinModelPortali Q63151.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63151. 1 interaction.
STRINGi 10116.ENSRNOP00000020161.

Proteomic databases

PaxDbi Q63151.
PRIDEi Q63151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000020161 ; ENSRNOP00000020161 ; ENSRNOG00000014718 . [Q63151-1 ]
GeneIDi 114024.
KEGGi rno:114024.
UCSCi RGD:70552. rat. [Q63151-1 ]

Organism-specific databases

CTDi 2181.
RGDi 70552. Acsl3.

Phylogenomic databases

eggNOGi COG1022.
GeneTreei ENSGT00690000102168.
HOGENOMi HOG000159459.
HOVERGENi HBG106947.
InParanoidi Q63151.
KOi K01897.
OMAi CDRFIFR.
OrthoDBi EOG7P2XRD.
PhylomeDBi Q63151.
TreeFami TF314012.

Enzyme and pathway databases

BRENDAi 6.2.1.3. 5301.

Miscellaneous databases

NextBioi 618179.
PROi Q63151.

Gene expression databases

Genevestigatori Q63151.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and expression of rat acyl-CoA synthetase 3."
    Fujino T., Kang M.-J., Suzuki H., Iijima H., Yamamoto T.T.
    J. Biol. Chem. 271:16748-16752(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: Wistar.
    Tissue: Brain.
  2. "Alternative translation initiation generates acyl-CoA synthetase 3 isoforms with heterogeneous amino termini."
    Fujino T., Kang M.-J., Minekura H., Suzuki H., Yamamoto T.T.
    J. Biochem. 122:212-216(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE INITIATION.
  3. "Suppression of long chain acyl-CoA synthetase 3 (ACSL3) decreases hepatic de novo fatty acid synthesis through decreased transcriptional activity."
    Bu S.Y., Mashek M.T., Mashek D.G.
    J. Biol. Chem. 284:30474-30483(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiACSL3_RAT
AccessioniPrimary (citable) accession number: Q63151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3