Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q63147

- HEM0_RAT

UniProt

Q63147 - HEM0_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

Alas2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631SubstrateBy similarity
Binding sitei280 – 2801SubstrateBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei332 – 3321Pyridoxal phosphateBy similarity
Binding sitei360 – 3601Pyridoxal phosphateBy similarity
Binding sitei388 – 3881Pyridoxal phosphateBy similarity
Active sitei391 – 3911By similarity
Binding sitei420 – 4201Pyridoxal phosphateBy similarity
Binding sitei421 – 4211Pyridoxal phosphateBy similarity
Binding sitei508 – 5081SubstrateBy similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: UniProtKB
  2. coenzyme binding Source: RGD
  3. glycine binding Source: RGD
  4. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. erythrocyte differentiation Source: UniProtKB
  2. female pregnancy Source: RGD
  3. heme biosynthetic process Source: UniProtKB
  4. hemoglobin biosynthetic process Source: UniProtKB
  5. lactation Source: RGD
  6. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  7. response to drug Source: RGD
  8. response to hypoxia Source: UniProtKB
  9. response to lead ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:Alas2
Synonyms:Alase
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2084. Alas2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial matrix Source: InterPro
  3. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – ?4949MitochondrionSequence AnalysisAdd
BLAST
Chaini?50 – 5875385-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000001225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei391 – 3911N6-(pyridoxal phosphate)lysineCurated

Proteomic databases

PRIDEiQ63147.

Expressioni

Tissue specificityi

Erythroid specific.

Gene expression databases

GenevestigatoriQ63147.

Interactioni

Subunit structurei

Interacts with SUCLA2 (By similarity). Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ63147.
SMRiQ63147. Positions 1-49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG005954.
InParanoidiQ63147.
KOiK00643.
PhylomeDBiQ63147.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAAAMLLRS CPVLSKGPTG LLGKVAKTYQ FLFGIGRCPI LATQGPTCSQ
60 70 80 90 100
IHLKATKAGA DSPSWTKSHC PFMLSELQDR KSKIVQRAAP EVQEDVKTFK
110 120 130 140 150
TDLLSFMEST TRSQSVPRFQ DPEQTGGAPP LLIQNNMTGS QAFGYDQFFR
160 170 180 190 200
DKIMEKKQDH TYRVFKTVNR WANAYPFAQH FSEASMDSKD VSVWCSNDYL
210 220 230 240 250
GISRHPRVLQ AIEETLKNHG AGAGGTRNIS GTSKFHVELE QELAELHHKD
260 270 280 290 300
SALLFSSCFV ANDSTLFTLA KLLPGCEIYS DAGNHASMIQ GIRNSGAVKF
310 320 330 340 350
VFRHNDPGHL KKLLEKSDPK TPKIVAFETV HSMDGAICPL EELCDVAHQY
360 370 380 390 400
GALTFVDEVH AVGLYGTRGA GIGERDGIMH KLDIISGTLG KAFGCVGGYI
410 420 430 440 450
ASTRDLVDMV RSYAAGFIFT TSLPPMVLSG ALESVRLLKG EEGQALRRAH
460 470 480 490 500
QRNVKHMRQL LMDRGFPVIP CPSHIIPIRV GNAALNSKIC DLLLAKHSIY
510 520 530 540 550
VQAINYPTVP RGEELLRLAP SPHHSPQMME NFVEKLLLAW TEVGLPLQDV
560 570 580
SVAACNFCRR PVHFELMSEW ERSYFGNMGP QYVTTYA
Length:587
Mass (Da):64,842
Last modified:November 1, 1996 - v1
Checksum:iA6287CA9543DECAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86297 mRNA. Translation: BAA13063.1.
PIRiJX0278.
RefSeqiNP_037329.1. NM_013197.1.
UniGeneiRn.226279.
Rn.32517.

Genome annotation databases

GeneIDi25748.
KEGGirno:25748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86297 mRNA. Translation: BAA13063.1 .
PIRi JX0278.
RefSeqi NP_037329.1. NM_013197.1.
UniGenei Rn.226279.
Rn.32517.

3D structure databases

ProteinModelPortali Q63147.
SMRi Q63147. Positions 1-49.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q63147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25748.
KEGGi rno:25748.

Organism-specific databases

CTDi 212.
RGDi 2084. Alas2.

Phylogenomic databases

HOVERGENi HBG005954.
InParanoidi Q63147.
KOi K00643.
PhylomeDBi Q63147.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00375 .

Miscellaneous databases

NextBioi 607929.
PROi Q63147.

Gene expression databases

Genevestigatori Q63147.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and structure of rat erythroid-specific delta-aminolevulinate synthase."
    Munakata H., Yamagami T., Nagai T., Yamamoto M., Hayashi N.
    J. Biochem. 114:103-111(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  2. Furuyama K.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiHEM0_RAT
AccessioniPrimary (citable) accession number: Q63147
Secondary accession number(s): Q63895
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3