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Q63147

- HEM0_RAT

UniProt

Q63147 - HEM0_RAT

Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

Alas2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei163 – 1631SubstrateBy similarity
    Binding sitei280 – 2801SubstrateBy similarity
    Binding sitei299 – 2991SubstrateBy similarity
    Binding sitei332 – 3321Pyridoxal phosphateBy similarity
    Binding sitei360 – 3601Pyridoxal phosphateBy similarity
    Binding sitei388 – 3881Pyridoxal phosphateBy similarity
    Active sitei391 – 3911By similarity
    Binding sitei420 – 4201Pyridoxal phosphateBy similarity
    Binding sitei421 – 4211Pyridoxal phosphateBy similarity
    Binding sitei508 – 5081SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: UniProtKB
    2. coenzyme binding Source: RGD
    3. glycine binding Source: RGD
    4. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. erythrocyte differentiation Source: UniProtKB
    2. female pregnancy Source: RGD
    3. heme biosynthetic process Source: UniProtKB
    4. hemoglobin biosynthetic process Source: UniProtKB
    5. lactation Source: RGD
    6. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
    7. response to drug Source: RGD
    8. response to hypoxia Source: UniProtKB
    9. response to lead ion Source: RGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
    Short name:
    ALAS-E
    Alternative name(s):
    5-aminolevulinic acid synthase 2
    Delta-ALA synthase 2
    Delta-aminolevulinate synthase 2
    Gene namesi
    Name:Alas2
    Synonyms:Alase
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2084. Alas2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – ?4949MitochondrionSequence AnalysisAdd
    BLAST
    Chaini?50 – 5875385-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000001225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei391 – 3911N6-(pyridoxal phosphate)lysineCurated

    Proteomic databases

    PRIDEiQ63147.

    Expressioni

    Tissue specificityi

    Erythroid specific.

    Gene expression databases

    GenevestigatoriQ63147.

    Interactioni

    Subunit structurei

    Interacts with SUCLA2 By similarity. Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ63147.
    SMRiQ63147. Positions 1-49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG005954.
    KOiK00643.
    PhylomeDBiQ63147.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63147-1 [UniParc]FASTAAdd to Basket

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    MVAAAMLLRS CPVLSKGPTG LLGKVAKTYQ FLFGIGRCPI LATQGPTCSQ    50
    IHLKATKAGA DSPSWTKSHC PFMLSELQDR KSKIVQRAAP EVQEDVKTFK 100
    TDLLSFMEST TRSQSVPRFQ DPEQTGGAPP LLIQNNMTGS QAFGYDQFFR 150
    DKIMEKKQDH TYRVFKTVNR WANAYPFAQH FSEASMDSKD VSVWCSNDYL 200
    GISRHPRVLQ AIEETLKNHG AGAGGTRNIS GTSKFHVELE QELAELHHKD 250
    SALLFSSCFV ANDSTLFTLA KLLPGCEIYS DAGNHASMIQ GIRNSGAVKF 300
    VFRHNDPGHL KKLLEKSDPK TPKIVAFETV HSMDGAICPL EELCDVAHQY 350
    GALTFVDEVH AVGLYGTRGA GIGERDGIMH KLDIISGTLG KAFGCVGGYI 400
    ASTRDLVDMV RSYAAGFIFT TSLPPMVLSG ALESVRLLKG EEGQALRRAH 450
    QRNVKHMRQL LMDRGFPVIP CPSHIIPIRV GNAALNSKIC DLLLAKHSIY 500
    VQAINYPTVP RGEELLRLAP SPHHSPQMME NFVEKLLLAW TEVGLPLQDV 550
    SVAACNFCRR PVHFELMSEW ERSYFGNMGP QYVTTYA 587
    Length:587
    Mass (Da):64,842
    Last modified:November 1, 1996 - v1
    Checksum:iA6287CA9543DECAF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86297 mRNA. Translation: BAA13063.1.
    PIRiJX0278.
    RefSeqiNP_037329.1. NM_013197.1.
    UniGeneiRn.226279.
    Rn.32517.

    Genome annotation databases

    GeneIDi25748.
    KEGGirno:25748.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86297 mRNA. Translation: BAA13063.1 .
    PIRi JX0278.
    RefSeqi NP_037329.1. NM_013197.1.
    UniGenei Rn.226279.
    Rn.32517.

    3D structure databases

    ProteinModelPortali Q63147.
    SMRi Q63147. Positions 1-49.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q63147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25748.
    KEGGi rno:25748.

    Organism-specific databases

    CTDi 212.
    RGDi 2084. Alas2.

    Phylogenomic databases

    HOVERGENi HBG005954.
    KOi K00643.
    PhylomeDBi Q63147.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .

    Miscellaneous databases

    NextBioi 607929.
    PROi Q63147.

    Gene expression databases

    Genevestigatori Q63147.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR015118. 5aminolev_synth_preseq.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    PF09029. Preseq_ALAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and structure of rat erythroid-specific delta-aminolevulinate synthase."
      Munakata H., Yamagami T., Nagai T., Yamamoto M., Hayashi N.
      J. Biochem. 114:103-111(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    2. Furuyama K.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiHEM0_RAT
    AccessioniPrimary (citable) accession number: Q63147
    Secondary accession number(s): Q63895
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3