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Protein

Complement component receptor 1-like protein

Gene

Cr1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. Also acts as a decay-accelerating factor, preventing the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Seems to act as a costimulatory factor for T-cells. May play a crucial role in early embryonic development by maintaining fetomaternal tolerance.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity, Pregnancy

Enzyme and pathway databases

ReactomeiR-RNO-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement component receptor 1-like protein
Alternative name(s):
Antigen 5I2
Complement regulatory protein Crry
Gene namesi
Name:Cr1l
Synonyms:Crry
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2399. Cr1l.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 482447ExtracellularSequence analysisAdd
BLAST
Transmembranei483 – 50321HelicalSequence analysisAdd
BLAST
Topological domaini504 – 55956CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 559524Complement component receptor 1-like proteinPRO_0000238979Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 81PROSITE-ProRule annotation1 Publication
Glycosylationi53 – 531O-linked (GalNAc...)Sequence analysis
Disulfide bondi68 ↔ 94PROSITE-ProRule annotation1 Publication
Disulfide bondi99 ↔ 140PROSITE-ProRule annotation1 Publication
Disulfide bondi126 ↔ 156PROSITE-ProRule annotation1 Publication
Disulfide bondi161 ↔ 210PROSITE-ProRule annotation1 Publication
Disulfide bondi190 ↔ 227PROSITE-ProRule annotation1 Publication
Disulfide bondi233 ↔ 275PROSITE-ProRule annotation1 Publication
Disulfide bondi261 ↔ 288PROSITE-ProRule annotation1 Publication
Disulfide bondi294 ↔ 336PROSITE-ProRule annotation
Disulfide bondi322 ↔ 352PROSITE-ProRule annotation
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi357 ↔ 400PROSITE-ProRule annotation
Disulfide bondi386 ↔ 413PROSITE-ProRule annotation
Disulfide bondi419 ↔ 462PROSITE-ProRule annotation
Disulfide bondi448 ↔ 475PROSITE-ProRule annotation
Modified residuei527 – 5271PhosphoserineCombined sources
Modified residuei531 – 5311PhosphoserineBy similarity
Modified residuei537 – 5371PhosphoserineBy similarity
Modified residuei540 – 5401PhosphothreonineBy similarity
Modified residuei554 – 5541PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63135.
PRIDEiQ63135.

PTM databases

iPTMnetiQ63135.
PhosphoSiteiQ63135.

Expressioni

Gene expression databases

ExpressionAtlasiQ63135. baseline and differential.
GenevisibleiQ63135. RN.

Interactioni

Subunit structurei

Interacts with C3b.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010798.

Structurei

Secondary structure

1
559
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi45 – 517Combined sources
Beta strandi63 – 686Combined sources
Beta strandi76 – 816Combined sources
Beta strandi108 – 1147Combined sources
Beta strandi121 – 1266Combined sources
Beta strandi130 – 1345Combined sources
Beta strandi136 – 1438Combined sources
Beta strandi146 – 1516Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi170 – 1734Combined sources
Beta strandi185 – 1906Combined sources
Beta strandi200 – 2045Combined sources
Beta strandi206 – 22217Combined sources
Beta strandi226 – 2294Combined sources
Beta strandi241 – 2433Combined sources
Beta strandi249 – 2524Combined sources
Beta strandi256 – 2616Combined sources
Beta strandi266 – 2694Combined sources
Beta strandi271 – 2766Combined sources
Turni277 – 2793Combined sources
Beta strandi280 – 2834Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NTJmodel-A34-353[»]
2XRBX-ray2.50A1-288[»]
2XRDX-ray3.50A1-288[»]
ProteinModelPortaliQ63135.
SMRiQ63135. Positions 97-228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63135.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 9661Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 15862Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini159 – 22971Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini231 – 29060Sushi 4PROSITE-ProRule annotationAdd
BLAST
Domaini292 – 35463Sushi 5PROSITE-ProRule annotationAdd
BLAST
Domaini355 – 41561Sushi 6PROSITE-ProRule annotationAdd
BLAST
Domaini417 – 47761Sushi 7PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 7 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000111977.
HOVERGENiHBG081346.
InParanoidiQ63135.
KOiK04005.
PhylomeDBiQ63135.
TreeFamiTF334137.

Family and domain databases

InterProiIPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 7 hits.
[Graphical view]
SMARTiSM00032. CCP. 7 hits.
[Graphical view]
SUPFAMiSSF57535. SSF57535. 7 hits.
PROSITEiPS50923. SUSHI. 7 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63135-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEASSPLDPV GRLVAFCRGG VHLAVLLLFL SPSTLGQCPA PPLFPYAKPI
60 70 80 90 100
NPTDESTFPV GTSLKYECRP GYIKRQFSIT CEVNSVWTSP QDVCIRKQCE
110 120 130 140 150
TPLDPQNGIV HVNTDIRFGS SITYTCNEGY RLIGSSSAMC IISDQSVAWD
160 170 180 190 200
AEAPICESIP CEIPPSIPNG DFFSPNREDF HYGMVVTYQC NTDARGKKLF
210 220 230 240 250
NLVGEPSIHC TSIDGQVGVW SGPPPQCIEL NKCTPPHVEN AVIVSKNKSL
260 270 280 290 300
FSLRDMVEFR CQDGFMMKGD SSVYCRSLNR WEPQLPSCFK VKSCGAFLGE
310 320 330 340 350
LPNGHVFVPQ NLQLGAKVTF VCNTGYQLKG NSSSHCVLDG VESIWNSSVP
360 370 380 390 400
VCEQVICKLP QDMSGFQKGL QMKKDYYYGD NVALECEDGY TLEGSSQSQC
410 420 430 440 450
QSDASWDPPL PKCVSQVICK LPQDMSGFQK GLQMKKDYYY GDNVALECED
460 470 480 490 500
GYTLEGSSQS QCQSDASWDP PLPKCVSRSN SGLIAGIFIG IIVLILFIIF
510 520 530 540 550
SYWMIMKFKK RNSTNEKCKE VGIYLNSKED SCVQPQSLLT SQENNSTSSP

ARNSLTQEV
Length:559
Mass (Da):61,680
Last modified:November 1, 1996 - v1
Checksum:i29E10F6A21DB9B6E
GO
Isoform 2 (identifier: Q63135-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     354-415: Missing.

Show »
Length:497
Mass (Da):54,787
Checksum:i177AC11EE0F1AD1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661Y → Q AA sequence (PubMed:8144902).Curated
Sequence conflicti69 – 691R → Q AA sequence (PubMed:8144902).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei354 – 41562Missing in isoform 2. 1 PublicationVSP_019051Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42114 mRNA. Translation: BAA07698.1.
D42115 mRNA. Translation: BAA22548.1.
L36532 mRNA. Translation: AAA91821.1.
BC061736 mRNA. Translation: AAH61736.1.
PIRiJC2054.
RefSeqiNP_001005265.1. NM_001005265.1. [Q63135-2]
NP_001005330.1. NM_001005330.1.
NP_062174.1. NM_019301.2. [Q63135-1]
UniGeneiRn.5825.

Genome annotation databases

EnsembliENSRNOT00000010799; ENSRNOP00000010798; ENSRNOG00000008193. [Q63135-1]
ENSRNOT00000084320; ENSRNOP00000072622; ENSRNOG00000008193. [Q63135-2]
GeneIDi54243.
KEGGirno:54243.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42114 mRNA. Translation: BAA07698.1.
D42115 mRNA. Translation: BAA22548.1.
L36532 mRNA. Translation: AAA91821.1.
BC061736 mRNA. Translation: AAH61736.1.
PIRiJC2054.
RefSeqiNP_001005265.1. NM_001005265.1. [Q63135-2]
NP_001005330.1. NM_001005330.1.
NP_062174.1. NM_019301.2. [Q63135-1]
UniGeneiRn.5825.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NTJmodel-A34-353[»]
2XRBX-ray2.50A1-288[»]
2XRDX-ray3.50A1-288[»]
ProteinModelPortaliQ63135.
SMRiQ63135. Positions 97-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010798.

PTM databases

iPTMnetiQ63135.
PhosphoSiteiQ63135.

Proteomic databases

PaxDbiQ63135.
PRIDEiQ63135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010799; ENSRNOP00000010798; ENSRNOG00000008193. [Q63135-1]
ENSRNOT00000084320; ENSRNOP00000072622; ENSRNOG00000008193. [Q63135-2]
GeneIDi54243.
KEGGirno:54243.

Organism-specific databases

CTDi1379.
RGDi2399. Cr1l.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118803.
HOGENOMiHOG000111977.
HOVERGENiHBG081346.
InParanoidiQ63135.
KOiK04005.
PhylomeDBiQ63135.
TreeFamiTF334137.

Enzyme and pathway databases

ReactomeiR-RNO-977606. Regulation of Complement cascade.

Miscellaneous databases

EvolutionaryTraceiQ63135.
NextBioi610712.
PROiQ63135.

Gene expression databases

ExpressionAtlasiQ63135. baseline and differential.
GenevisibleiQ63135. RN.

Family and domain databases

InterProiIPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 7 hits.
[Graphical view]
SMARTiSM00032. CCP. 7 hits.
[Graphical view]
SUPFAMiSSF57535. SSF57535. 7 hits.
PROSITEiPS50923. SUSHI. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the rat complement regulatory protein, 5I2 antigen."
    Sakurada C., Seno H., Dohi N., Takizawa H., Nonaka M., Okada N., Okada H.
    Biochem. Biophys. Res. Commun. 198:819-826(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Liver.
  2. "Molecular characterization of rat Crry: widespread distribution of two alternative forms of Crry mRNA."
    Quigg R.J., Lo C.F., Alexander J.J., Sneed A.E. III, Moxley G.
    Immunogenetics 42:362-367(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Prostate.
  4. "Complement inhibitor of rat cell membrane resembling mouse Crry/p65."
    Takizawa H., Okada N., Okada H.
    J. Immunol. 152:3032-3038(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-53; 66-74; 369-373 AND 520-528 (ISOFORM 1), INTERACTION WITH C3B, FUNCTION.
  5. "Crry and CD59 regulate complement in rat glomerular epithelial cells and are inhibited by the nephritogenic antibody of passive Heymann nephritis."
    Quigg R.J., Holers V.M., Morgan B.P., Sneed A.E. III
    J. Immunol. 154:3437-3443(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes."
    Antic Stankovic J., Vucevic D., Majstorovic I., Vasilijic S., Colic M.
    Life Sci. 75:3053-3062(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527 AND SER-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "The extended multidomain solution structures of the complement protein Crry and its chimeric conjugate Crry-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy."
    Aslam M., Guthridge J.M., Hack B.K., Quigg R.J., Holers V.M., Perkins S.J.
    J. Mol. Biol. 329:525-550(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 34-353.
  9. Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-288, DISULFIDE BONDS.

Entry informationi

Entry nameiCR1L_RAT
AccessioniPrimary (citable) accession number: Q63135
Secondary accession number(s): Q63612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.