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Protein

Canalicular multispecific organic anion transporter 1

Gene

Abcc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates hepatobiliary excretion of numerous organic anions.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi667 – 6748ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1330 – 13378ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • organic anion transmembrane transporter activity Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

  • cellular chloride ion homeostasis Source: RGD
  • drug transmembrane transport Source: RGD
  • organic anion transport Source: RGD
  • prostaglandin transport Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to drug Source: RGD
  • response to estrogen Source: RGD
  • response to heat Source: RGD
  • response to methotrexate Source: RGD
  • response to oxidative stress Source: RGD
  • response to steroid hormone Source: RGD
  • thyroid hormone transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-382556. ABC-family proteins mediated transport.
SABIO-RKQ63120.

Names & Taxonomyi

Protein namesi
Recommended name:
Canalicular multispecific organic anion transporter 1
Alternative name(s):
ATP-binding cassette sub-family C member 2
Canalicular multidrug resistance protein
Multidrug resistance-associated protein 2
Gene namesi
Name:Abcc2
Synonyms:Cmoat, Cmrp, Mrp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2366. Abcc2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626ExtracellularBy similarityAdd
BLAST
Transmembranei27 – 4721Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini48 – 6720CytoplasmicBy similarityAdd
BLAST
Transmembranei68 – 8821Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini89 – 924ExtracellularBy similarity
Transmembranei93 – 11321Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini114 – 12512CytoplasmicBy similarityAdd
BLAST
Transmembranei126 – 14621Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini147 – 16418ExtracellularBy similarityAdd
BLAST
Transmembranei165 – 18521Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini186 – 309124CytoplasmicBy similarityAdd
BLAST
Transmembranei310 – 33021Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini331 – 35626ExtracellularBy similarityAdd
BLAST
Transmembranei357 – 37721Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini378 – 43356CytoplasmicBy similarityAdd
BLAST
Transmembranei434 – 45421Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini455 – 4573ExtracellularBy similarity
Transmembranei458 – 47821Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini479 – 54062CytoplasmicBy similarityAdd
BLAST
Transmembranei541 – 56121Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Topological domaini562 – 58322ExtracellularBy similarityAdd
BLAST
Transmembranei584 – 60421Helical; Name=11PROSITE-ProRule annotationAdd
BLAST
Topological domaini605 – 967363CytoplasmicBy similarityAdd
BLAST
Transmembranei968 – 98821Helical; Name=12PROSITE-ProRule annotationAdd
BLAST
Topological domaini989 – 102941ExtracellularBy similarityAdd
BLAST
Transmembranei1030 – 105021Helical; Name=13PROSITE-ProRule annotationAdd
BLAST
Topological domaini1051 – 109343CytoplasmicBy similarityAdd
BLAST
Transmembranei1094 – 111421Helical; Name=14PROSITE-ProRule annotationAdd
BLAST
Topological domaini1115 – 11151ExtracellularBy similarity
Transmembranei1116 – 113621Helical; Name=15PROSITE-ProRule annotationAdd
BLAST
Topological domaini1137 – 120771CytoplasmicBy similarityAdd
BLAST
Transmembranei1208 – 122821Helical; Name=16PROSITE-ProRule annotationAdd
BLAST
Topological domaini1229 – 12302ExtracellularBy similarity
Transmembranei1231 – 125121Helical; Name=17PROSITE-ProRule annotationAdd
BLAST
Topological domaini1252 – 1541290CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • cell surface Source: Ensembl
  • integral component of plasma membrane Source: Ensembl
  • intercellular canaliculus Source: Ensembl
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Abcc2 are a cause of hereditary conjugated hyperbilirubinemia (EHBR).

Chemistry

ChEMBLiCHEMBL2073676.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15411541Canalicular multispecific organic anion transporter 1PRO_0000093359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Modified residuei279 – 2791PhosphoserineCombined sources
Modified residuei281 – 2811PhosphoserineCombined sources
Modified residuei874 – 8741PhosphoserineCombined sources
Glycosylationi1007 – 10071N-linked (GlcNAc...)Sequence analysis
Glycosylationi1010 – 10101N-linked (GlcNAc...)Sequence analysis
Glycosylationi1011 – 10111N-linked (GlcNAc...)Sequence analysis
Modified residuei1434 – 14341PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63120.
PRIDEiQ63120.

PTM databases

iPTMnetiQ63120.
PhosphoSiteiQ63120.

Expressioni

Tissue specificityi

Mainly expressed in the liver.

Gene expression databases

GenevisibleiQ63120. RN.

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ63120. 1 interaction.
MINTiMINT-7138043.
STRINGi10116.ENSRNOP00000064799.

Chemistry

BindingDBiQ63120.

Structurei

3D structure databases

ProteinModelPortaliQ63120.
SMRiQ63120. Positions 632-859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini318 – 601284ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini633 – 857225ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini975 – 1260286ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1296 – 1530235ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00840000129727.
HOVERGENiHBG108314.
InParanoidiQ63120.
KOiK05666.
OMAiCNSTFWD.
OrthoDBiEOG7MWGW0.
PhylomeDBiQ63120.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030247. ABCC2.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF176. PTHR24223:SF176. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKFCNSTFW DLSLLESPEA DLPLCFEQTV LVWIPLGFLW LLAPWQLYSV
60 70 80 90 100
YRSRTKRSSI TKFYLAKQVF VVFLLILAAI DLSLALTEDT GQATVPPVRY
110 120 130 140 150
TNPILYLCTW LLVLAVQHSR QWCVRKNSWF LSLFWILSVL CGVFQFQTLI
160 170 180 190 200
RALLKDSKSN MAYSYLFFVS YGFQIVLLIL TAFSGPSDST QTPSVTASFL
210 220 230 240 250
SSITFSWYDR TVLKGYKHPL TLEDVWDIDE GFKTRSVTSK FEAAMTKDLQ
260 270 280 290 300
KARQAFQRRL QKSQRKPEAT LHGLNKKQSQ SQDVLVLEEA KKKSEKTTKD
310 320 330 340 350
YPKSWLIKSL FKTFHVVILK SFILKLIHDL LVFLNPQLLK LLIGFVKSSN
360 370 380 390 400
SYVWFGYICA ILMFAVTLIQ SFCLQSYFQH CFVLGMCVRT TVMSSIYKKA
410 420 430 440 450
LTLSNLARKQ YTIGETVNLM SVDSQKLMDA TNYMQLVWSS VIQITLSIFF
460 470 480 490 500
LWRELGPSIL AGVGVMVLLI PVNGVLATKI RNIQVQNMKN KDKRLKIMNE
510 520 530 540 550
ILSGIKILKY FAWEPSFQEQ VQGIRKKELK NLLRFGQLQS LLIFILQITP
560 570 580 590 600
ILVSVVTFSV YVLVDSANVL NAEKAFTSIT LFNILRFPLS MLPMVTSSIL
610 620 630 640 650
QASVSVDRLE RYLGGDDLDT SAIRRVSNFD KAVKFSEASF TWDPDLEATI
660 670 680 690 700
QDVNLDIKPG QLVAVVGTVG SGKSSLVSAM LGEMENVHGH ITIQGSTAYV
710 720 730 740 750
PQQSWIQNGT IKDNILFGSE YNEKKYQQVL KACALLPDLE ILPGGDMAEI
760 770 780 790 800
GEKGINLSGG QKQRVSLARA AYQDADIYIL DDPLSAVDAH VGKHIFNKVV
810 820 830 840 850
GPNGLLAGKT RIFVTHGIHF LPQVDEIVVL GKGTILEKGS YRDLLDKKGV
860 870 880 890 900
FARNWKTFMK HSGPEGEATV NNDSEAEDDD DGLIPTMEEI PEDAASLAMR
910 920 930 940 950
RENSLRRTLS RSSRSSSRRG KSLKNSLKIK NVNVLKEKEK EVEGQKLIKK
960 970 980 990 1000
EFVETGKVKF SIYLKYLQAV GWWSILFIIL FYGLNNVAFI GSNLWLSAWT
1010 1020 1030 1040 1050
SDSDNLNGTN NSSSHRDMRI GVFGALGLAQ GICLLISTLW SIYACRNASK
1060 1070 1080 1090 1100
ALHGQLLTNI LRAPMRFFDT TPTGRIVNRF SGDISTVDDL LPQTLRSWMM
1110 1120 1130 1140 1150
CFFGIAGTLV MICMATPVFA IIIIPLSILY ISVQVFYVAT SRQLRRLDSV
1160 1170 1180 1190 1200
TKSPIYSHFS ETVTGLPIIR AFEHQQRFLA WNEKQIDINQ KCVFSWITSN
1210 1220 1230 1240 1250
RWLAIRLELV GNLVVFCSAL LLVIYRKTLT GDVVGFVLSN ALNITQTLNW
1260 1270 1280 1290 1300
LVRMTSEAET NIVAVERISE YINVENEAPW VTDKRPPADW PRHGEIQFNN
1310 1320 1330 1340 1350
YQVRYRPELD LVLKGITCNI KSGEKVGVVG RTGAGKSSLT NCLFRILESA
1360 1370 1380 1390 1400
GGQIIIDGID VASIGLHDLR ERLTIIPQDP ILFSGSLRMN LDPFNKYSDE
1410 1420 1430 1440 1450
EVWRALELAH LRSFVSGLQL GLLSEVTEGG DNLSIGQRQL LCLGRAVLRK
1460 1470 1480 1490 1500
SKILVLDEAT AAVDLETDSL IQTTIRKEFS QCTVITIAHR LHTIMDSDKI
1510 1520 1530 1540
MVLDNGKIVE YGSPEELLSN RGSFYLMAKE AGIENVNHTE L
Length:1,541
Mass (Da):173,384
Last modified:November 1, 1996 - v1
Checksum:iD5FB55571BFDDB39
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti420 – 4201M → V in BAA13016 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L49379 mRNA. Translation: AAC42087.1.
X96393 mRNA. Translation: CAA65257.1.
D86086 mRNA. Translation: BAA13016.1.
PIRiS71839.
RefSeqiNP_036965.1. NM_012833.2.
UniGeneiRn.10265.

Genome annotation databases

EnsembliENSRNOT00000070861; ENSRNOP00000064799; ENSRNOG00000046727.
GeneIDi25303.
KEGGirno:25303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L49379 mRNA. Translation: AAC42087.1.
X96393 mRNA. Translation: CAA65257.1.
D86086 mRNA. Translation: BAA13016.1.
PIRiS71839.
RefSeqiNP_036965.1. NM_012833.2.
UniGeneiRn.10265.

3D structure databases

ProteinModelPortaliQ63120.
SMRiQ63120. Positions 632-859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63120. 1 interaction.
MINTiMINT-7138043.
STRINGi10116.ENSRNOP00000064799.

Chemistry

BindingDBiQ63120.
ChEMBLiCHEMBL2073676.

PTM databases

iPTMnetiQ63120.
PhosphoSiteiQ63120.

Proteomic databases

PaxDbiQ63120.
PRIDEiQ63120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000070861; ENSRNOP00000064799; ENSRNOG00000046727.
GeneIDi25303.
KEGGirno:25303.

Organism-specific databases

CTDi1244.
RGDi2366. Abcc2.

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00840000129727.
HOVERGENiHBG108314.
InParanoidiQ63120.
KOiK05666.
OMAiCNSTFWD.
OrthoDBiEOG7MWGW0.
PhylomeDBiQ63120.

Enzyme and pathway databases

ReactomeiR-RNO-382556. ABC-family proteins mediated transport.
SABIO-RKQ63120.

Miscellaneous databases

NextBioi606087.
PROiQ63120.

Gene expression databases

GenevisibleiQ63120. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030247. ABCC2.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF176. PTHR24223:SF176. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene."
    Paulusma C.C., Bosma P.J., Zaman G.J.R., Bakker C.T.M., Otter M., Scheffer G.L., Scheper R.J., Borst P., Oude Elferink R.P.J.
    Science 271:1126-1128(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats."
    Buechler M., Koenig J., Brom M., Kartenbeck J., Spring H., Horie T., Keppler D.
    J. Biol. Chem. 271:15091-15098(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INVOLVEMENT IN EHBR.
    Strain: Wistar.
    Tissue: Liver.
  3. "Expression of the putative ATP-binding cassette region, homologous to that in multidrug resistance associated protein (MRP), is hereditarily defective in Eisai hyperbilirubinemic rats (EHBR)."
    Ito K., Suzuki H., Hirohashi T., Kume K., Shimizu T., Sugiyama Y.
    Int. Hepatol. Commun. 292:292-299(1996)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INVOLVEMENT IN EHBR.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-279; SER-281 AND SER-874, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMRP2_RAT
AccessioniPrimary (citable) accession number: Q63120
Secondary accession number(s): Q63145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.