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Protein

Dual specificity protein kinase CLK3

Gene

Clk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Leucettine L41 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186ATPPROSITE-ProRule annotation1
Active sitei283Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 170ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK3 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 3
Gene namesi
Name:Clk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621259. Clk3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858721 – 490Dual specificity protein kinase CLK3Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphotyrosineBy similarity1
Modified residuei9PhosphoserineCombined sources1
Modified residuei49PhosphoserineBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei76PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei135PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylates on all three types of residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63117.
PRIDEiQ63117.

PTM databases

iPTMnetiQ63117.
PhosphoSitePlusiQ63117.

Expressioni

Gene expression databases

BgeeiENSRNOG00000030126.
GenevisibleiQ63117. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061691.

Structurei

3D structure databases

ProteinModelPortaliQ63117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 472Protein kinasePROSITE-ProRule annotationAdd BLAST317

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ63117.
KOiK08823.
OMAiNENKSCE.
PhylomeDBiQ63117.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHCKRYRSP EPDPYLSYRW KRRRSYSREH EGRLRYPSRR EPPPRRSRSR
60 70 80 90 100
SHDRIPYQRR YREHRDSDTY RCEERSPSFG EDCYGSSRSR HRRRSRERGP
110 120 130 140 150
YRTRKHAHHC HKRRTRSCSS ASSRSQQSSK RSSRSVEDDK EGHLVCRIGD
160 170 180 190 200
WLQERYEIVG NLGEGTFGKV VECLDHARGK SQVALKIIRN VGKYREAARL
210 220 230 240 250
EINVLKKIKE KDKENKFLCV LMSDWFNFHG HMCIAFELLG KNTFEFLKEN
260 270 280 290 300
NFQPYPLPHV RHMAYQLCHA LRFLHENQLT HTDLKPENIL FVNSEFETLY
310 320 330 340 350
NEHKSCEEKS VKNTSIRVAD FGSATFDHEH HTTIVATRHY RPPEVILELG
360 370 380 390 400
WAQPCDVWSI GCILFEYYRG FTLFQTHENR EHLVMMEKIL GPIPSHMIHR
410 420 430 440 450
TRKQKYFYKG GLVWDENSSD GRYVKENCKP LKSYMLQDSL EHVQLFDLMR
460 470 480 490
RMLEFDPAQR ITLAEALLHP FFAGLTPEER SFHSSRNPSR
Length:490
Mass (Da):58,485
Last modified:June 12, 2007 - v2
Checksum:i6B10CA96A64AB19A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti458A → S in CAA64076 (PubMed:8679717).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94351 mRNA. Translation: CAA64076.1.
BC070891 mRNA. Translation: AAH70891.1.
PIRiS70352.
RefSeqiNP_599167.1. NM_134340.1.
UniGeneiRn.10340.

Genome annotation databases

EnsembliENSRNOT00000068108; ENSRNOP00000061691; ENSRNOG00000030126.
ENSRNOT00000080071; ENSRNOP00000072998; ENSRNOG00000030126.
GeneIDi171305.
KEGGirno:171305.
UCSCiRGD:621259. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94351 mRNA. Translation: CAA64076.1.
BC070891 mRNA. Translation: AAH70891.1.
PIRiS70352.
RefSeqiNP_599167.1. NM_134340.1.
UniGeneiRn.10340.

3D structure databases

ProteinModelPortaliQ63117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061691.

PTM databases

iPTMnetiQ63117.
PhosphoSitePlusiQ63117.

Proteomic databases

PaxDbiQ63117.
PRIDEiQ63117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068108; ENSRNOP00000061691; ENSRNOG00000030126.
ENSRNOT00000080071; ENSRNOP00000072998; ENSRNOG00000030126.
GeneIDi171305.
KEGGirno:171305.
UCSCiRGD:621259. rat.

Organism-specific databases

CTDi1198.
RGDi621259. Clk3.

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ63117.
KOiK08823.
OMAiNENKSCE.
PhylomeDBiQ63117.

Enzyme and pathway databases

BRENDAi2.7.12.1. 5301.

Miscellaneous databases

PROiQ63117.

Gene expression databases

BgeeiENSRNOG00000030126.
GenevisibleiQ63117. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLK3_RAT
AccessioniPrimary (citable) accession number: Q63117
Secondary accession number(s): Q6IRK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.