Dual specificity protein kinase CLK3 - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

Q63117 (CLK3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dual specificity protein kinase CLK3
EC=2.7.12.1

Alternative name(s):
CDC-like kinase 3

Gene names

Name:

Clk3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Leucettine L41 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cytoplasmic vesicle › secretory vesicle › acrosome By similarity.
Post-translational modification	Autophosphorylates on all three types of residues.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of RNA splicing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 490

490

Dual specificity protein kinase CLK3

PRO_0000085872

Regions

Domain

156 – 472

317

Protein kinase

Nucleotide binding

162 – 170

ATP By similarity

Sites

Active site

283

Proton acceptor By similarity

Binding site

186

ATP By similarity

Amino acid modifications

Modified residue

Phosphotyrosine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

135

Phosphoserine By similarity

Experimental info

Sequence conflict

458

A → S in CAA64076. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q63117 [UniParc].

Last modified June 12, 2007. Version 2.
Checksum: 6B10CA96A64AB19A
Show »

FASTA

490

58,485

        10         20         30         40         50         60 
MHHCKRYRSP EPDPYLSYRW KRRRSYSREH EGRLRYPSRR EPPPRRSRSR SHDRIPYQRR 

        70         80         90        100        110        120 
YREHRDSDTY RCEERSPSFG EDCYGSSRSR HRRRSRERGP YRTRKHAHHC HKRRTRSCSS 

       130        140        150        160        170        180 
ASSRSQQSSK RSSRSVEDDK EGHLVCRIGD WLQERYEIVG NLGEGTFGKV VECLDHARGK 

       190        200        210        220        230        240 
SQVALKIIRN VGKYREAARL EINVLKKIKE KDKENKFLCV LMSDWFNFHG HMCIAFELLG 

       250        260        270        280        290        300 
KNTFEFLKEN NFQPYPLPHV RHMAYQLCHA LRFLHENQLT HTDLKPENIL FVNSEFETLY 

       310        320        330        340        350        360 
NEHKSCEEKS VKNTSIRVAD FGSATFDHEH HTTIVATRHY RPPEVILELG WAQPCDVWSI 

       370        380        390        400        410        420 
GCILFEYYRG FTLFQTHENR EHLVMMEKIL GPIPSHMIHR TRKQKYFYKG GLVWDENSSD 

       430        440        450        460        470        480 
GRYVKENCKP LKSYMLQDSL EHVQLFDLMR RMLEFDPAQR ITLAEALLHP FFAGLTPEER 

       490 
SFHSSRNPSR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis." Becker W., Kentrup H., Heukelbach J., Joost H.G. Biochim. Biophys. Acta 1312:63-67(1996) [PubMed: 8679717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94351 mRNA. Translation: CAA64076.1. BC070891 mRNA. Translation: AAH70891.1.
IPI	IPI00947828.
PIR	S70352.
RefSeq	NP_599167.1. NM_134340.1.
UniGene	Rn.10340.
3D structure databases
ProteinModelPortal	Q63117.
SMR	Q63117. Positions 136-481.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63117.
PTM databases
PhosphoSite	Q63117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000068108; ENSRNOP00000061691; ENSRNOG00000030126.
GeneID	171305.
KEGG	rno:171305.
UCSC	NM_134340. rat.
Organism-specific databases
CTD	1198.
RGD	621259. Clk3.
Phylogenomic databases
eggNOG	roNOG09009.
GeneTree	ENSGT00580000081366.
HOVERGEN	HBG107720.
InParanoid	Q63117.
OrthoDB	EOG49ZXP2.
PhylomeDB	Q63117.
Enzyme and pathway databases
BRENDA	2.7.12.1. 5301.
Gene expression databases
ArrayExpress	Q63117.
Genevestigator	Q63117.
GermOnline	ENSRNOG00000030126. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08823.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622075.

Entry information

Entry name

CLK3_RAT

Accession

Primary (citable) accession number: Q63117
Secondary accession number(s): Q6IRK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents