Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dual specificity protein kinase CLK3

Gene

Clk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Leucettine L41 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei186 – 1861ATPPROSITE-ProRule annotation
Active sitei283 – 2831Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1709ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK3 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 3
Gene namesi
Name:Clk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621259. Clk3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Dual specificity protein kinase CLK3PRO_0000085872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphotyrosineBy similarity
Modified residuei9 – 91PhosphoserineCombined sources
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei76 – 761PhosphoserineBy similarity
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei135 – 1351PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylates on all three types of residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63117.
PRIDEiQ63117.

PTM databases

iPTMnetiQ63117.
PhosphoSiteiQ63117.

Expressioni

Gene expression databases

GenevisibleiQ63117. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061691.

Structurei

3D structure databases

ProteinModelPortaliQ63117.
SMRiQ63117. Positions 136-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini156 – 472317Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ63117.
KOiK08823.
OMAiNENKSCE.
OrthoDBiEOG7TBC1V.
PhylomeDBiQ63117.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHCKRYRSP EPDPYLSYRW KRRRSYSREH EGRLRYPSRR EPPPRRSRSR
60 70 80 90 100
SHDRIPYQRR YREHRDSDTY RCEERSPSFG EDCYGSSRSR HRRRSRERGP
110 120 130 140 150
YRTRKHAHHC HKRRTRSCSS ASSRSQQSSK RSSRSVEDDK EGHLVCRIGD
160 170 180 190 200
WLQERYEIVG NLGEGTFGKV VECLDHARGK SQVALKIIRN VGKYREAARL
210 220 230 240 250
EINVLKKIKE KDKENKFLCV LMSDWFNFHG HMCIAFELLG KNTFEFLKEN
260 270 280 290 300
NFQPYPLPHV RHMAYQLCHA LRFLHENQLT HTDLKPENIL FVNSEFETLY
310 320 330 340 350
NEHKSCEEKS VKNTSIRVAD FGSATFDHEH HTTIVATRHY RPPEVILELG
360 370 380 390 400
WAQPCDVWSI GCILFEYYRG FTLFQTHENR EHLVMMEKIL GPIPSHMIHR
410 420 430 440 450
TRKQKYFYKG GLVWDENSSD GRYVKENCKP LKSYMLQDSL EHVQLFDLMR
460 470 480 490
RMLEFDPAQR ITLAEALLHP FFAGLTPEER SFHSSRNPSR
Length:490
Mass (Da):58,485
Last modified:June 12, 2007 - v2
Checksum:i6B10CA96A64AB19A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti458 – 4581A → S in CAA64076 (PubMed:8679717).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94351 mRNA. Translation: CAA64076.1.
BC070891 mRNA. Translation: AAH70891.1.
PIRiS70352.
RefSeqiNP_599167.1. NM_134340.1.
XP_006243211.1. XM_006243149.2.
UniGeneiRn.10340.

Genome annotation databases

EnsembliENSRNOT00000068108; ENSRNOP00000061691; ENSRNOG00000030126.
ENSRNOT00000080071; ENSRNOP00000072998; ENSRNOG00000030126.
GeneIDi171305.
KEGGirno:171305.
UCSCiRGD:621259. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94351 mRNA. Translation: CAA64076.1.
BC070891 mRNA. Translation: AAH70891.1.
PIRiS70352.
RefSeqiNP_599167.1. NM_134340.1.
XP_006243211.1. XM_006243149.2.
UniGeneiRn.10340.

3D structure databases

ProteinModelPortaliQ63117.
SMRiQ63117. Positions 136-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061691.

PTM databases

iPTMnetiQ63117.
PhosphoSiteiQ63117.

Proteomic databases

PaxDbiQ63117.
PRIDEiQ63117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068108; ENSRNOP00000061691; ENSRNOG00000030126.
ENSRNOT00000080071; ENSRNOP00000072998; ENSRNOG00000030126.
GeneIDi171305.
KEGGirno:171305.
UCSCiRGD:621259. rat.

Organism-specific databases

CTDi1198.
RGDi621259. Clk3.

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ63117.
KOiK08823.
OMAiNENKSCE.
OrthoDBiEOG7TBC1V.
PhylomeDBiQ63117.

Enzyme and pathway databases

BRENDAi2.7.12.1. 5301.

Miscellaneous databases

PROiQ63117.

Gene expression databases

GenevisibleiQ63117. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis."
    Becker W., Kentrup H., Heukelbach J., Joost H.G.
    Biochim. Biophys. Acta 1312:63-67(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLK3_RAT
AccessioniPrimary (citable) accession number: Q63117
Secondary accession number(s): Q6IRK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 12, 2007
Last modified: June 8, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.