Reviewed, UniProtKB/Swiss-Prot Q63117 (CLK3_RAT)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase CLK3
    EC=2.7.12.1
Alternative name(s):
    CDC-like kinase 3

Gene names

Name:

Clk3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cytoplasmic vesicle › secretory vesicle › acrosome By similarity.
Post-translational modification	Autophosphorylates on all three types of residues.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Dual specificity protein kinase CLK3		PRO_0000085872
Regions
	Domain	156 – 472	317	Protein kinase
	Nucleotide binding	162 – 170	9	ATP By similarity
Sites
	Active site	283	1	Proton acceptor By similarity
	Binding site	186	1	ATP By similarity
Amino acid modifications
	Modified residue	7	1	Phosphotyrosine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	49	1	Phosphoserine By similarity
	Modified residue	51	1	Phosphoserine By similarity
	Modified residue	67	1	Phosphoserine By similarity
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	78	1	Phosphoserine By similarity
	Modified residue	135	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	458	1	A → S in CAA64076. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q63117-1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 6B10CA96A64AB19A	FASTA	490	58,485
	10 20 30 40 50 60 MHHCKRYRSP EPDPYLSYRW KRRRSYSREH EGRLRYPSRR EPPPRRSRSR SHDRIPYQRR 70 80 90 100 110 120 YREHRDSDTY RCEERSPSFG EDCYGSSRSR HRRRSRERGP YRTRKHAHHC HKRRTRSCSS 130 140 150 160 170 180 ASSRSQQSSK RSSRSVEDDK EGHLVCRIGD WLQERYEIVG NLGEGTFGKV VECLDHARGK 190 200 210 220 230 240 SQVALKIIRN VGKYREAARL EINVLKKIKE KDKENKFLCV LMSDWFNFHG HMCIAFELLG 250 260 270 280 290 300 KNTFEFLKEN NFQPYPLPHV RHMAYQLCHA LRFLHENQLT HTDLKPENIL FVNSEFETLY 310 320 330 340 350 360 NEHKSCEEKS VKNTSIRVAD FGSATFDHEH HTTIVATRHY RPPEVILELG WAQPCDVWSI 370 380 390 400 410 420 GCILFEYYRG FTLFQTHENR EHLVMMEKIL GPIPSHMIHR TRKQKYFYKG GLVWDENSSD 430 440 450 460 470 480 GRYVKENCKP LKSYMLQDSL EHVQLFDLMR RMLEFDPAQR ITLAEALLHP FFAGLTPEER 490 SFHSSRNPSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and characterization of rat Clk3, a LAMMER kinase predominately expressed in testis." Becker W., Kentrup H., Heukelbach J., Joost H.G. Biochim. Biophys. Acta 1312:63-67(1996) [PubMed: 8679717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94351 mRNA. Translation: CAA64076.1. BC070891 mRNA. Translation: AAH70891.1.
IPI	IPI00205776.
PIR	S70352.
RefSeq	NP_599167.1.
UniGene	Rn.10340
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
SMR	Q63117. Positions 136-481.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63117.
PTM databases
PhosphoSite	Q63117.
Genome annotation databases
Ensembl	ENSRNOT00000049440; ENSRNOP00000042962; ENSRNOG00000030126; Rattus norvegicus. [Genome view]
GeneID	171305.
KEGG	rno:171305.
UCSC	NM_134340. rat.
Organism-specific databases
CTD	171305.
RGD	621259. Clk3.
Phylogenomic databases
HOVERGEN	Q63117.
Enzyme and pathway databases
BRENDA	2.7.12.1. 248.
Gene expression databases
ArrayExpress	Q63117.
Genevestigator	Q63117.
GermOnline	ENSRNOG00000030126. Rattus norvegicus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622075.

Entry information

Entry name

CLK3_RAT

Accession

Primary (citable) accession number: Q63117
Secondary accession number(s): Q6IRK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 12, 2007
Last modified:	October 13, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)