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Protein

Cytoplasmic dynein 1 intermediate chain 1

Gene

Dync1i1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

GO - Molecular functioni

  • microtubule binding Source: HGNC
  • microtubule motor activity Source: HGNC

GO - Biological processi

  • vesicle transport along microtubule Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 1
Alternative name(s):
Cytoplasmic dynein intermediate chain 1
Dynein intermediate chain 1, cytosolic
Short name:
DH IC-1
Gene namesi
Name:Dync1i1
Synonyms:Dnci1, Dncic1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2512. Dync1i1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Dynein, Kinetochore, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 643643Cytoplasmic dynein 1 intermediate chain 1PRO_0000114654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei111 – 1111PhosphoserineCombined sources
Modified residuei174 – 1741PhosphothreonineBy similarity
Modified residuei177 – 1771PhosphoserineCombined sources
Modified residuei195 – 1951PhosphoserineCombined sources
Modified residuei633 – 6331PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63100.
PRIDEiQ63100.

PTM databases

iPTMnetiQ63100.
PhosphoSiteiQ63100.
SwissPalmiQ63100.

Expressioni

Tissue specificityi

High levels seen in the brain and testis, while a lower level expression is seen in the liver, spleen, kidney, lung, skeletal muscle and heart.

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Isoform 1, isoform 2 and isoform 3 interact with DYNC1H1. Isoform 1, isoform 2 and isoform 3 interact with DYNLT3. Isoform 1, isoform 2 and isoform 3 interact with DYNLT1. Interacts with DCTN1.By similarity6 Publications

GO - Molecular functioni

  • microtubule binding Source: HGNC

Protein-protein interaction databases

BioGridi248198. 6 interactions.
IntActiQ63100. 1 interaction.
MINTiMINT-4542819.
STRINGi10116.ENSRNOP00000013184.

Structurei

3D structure databases

ProteinModelPortaliQ63100.
SMRiQ63100. Positions 145-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati283 – 33250WD 1Add
BLAST
Repeati336 – 37641WD 2Add
BLAST
Repeati385 – 42642WD 3Add
BLAST
Repeati435 – 47541WD 4Add
BLAST
Repeati480 – 52546WD 5Add
BLAST
Repeati528 – 56841WD 6Add
BLAST
Repeati574 – 61340WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 123123Interaction with DCTN1Add
BLAST

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiQ63100.
KOiK10415.
PhylomeDBiQ63100.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63100-1) [UniParc]FASTAAdd to basket

Also known as: 1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVPDDS
60 70 80 90 100
DLDRKRRETE ALLQSIGISP EPPLVQPLHF LTWDTCYFHY LVPTPMSPSS
110 120 130 140 150
KSVSTPSEAG SQDDLGPLTR TLQWDTDPSV LQLQSDSELG RRLNKLGVSK
160 170 180 190 200
VTQVDFLPRE VVSYSKETQT PLATHQSEED EEDEEMVEPK VGHDSELENQ
210 220 230 240 250
DKKQETKEAP PRELTEEEKQ QILHSEEFLI FFDRTIRVIE RALAEDSDIF
260 270 280 290 300
FDYSGRELEE KDGDVQAGAN LSFNRQFYDE HWSKHRVVTC MDWSLQYPEL
310 320 330 340 350
MVASYSNNED APHEPDGVAL VWNMKFKKTT PEYVFHCQSS VMSVCFARFH
360 370 380 390 400
PNLVVGGTYS GQIVLWDNRS HRRTPVQRTP LSAAAHTHPV YCVNVVGTQN
410 420 430 440 450
AHNLITVSTD GKMCSWSLDM LSTPQESMEL VYNKSKPVAV TGMAFPTGDV
460 470 480 490 500
NNFVVGSEEG TVYTACRHGS KAGIGEVFEG HQGPVTGINC HMAVGPIDFS
510 520 530 540 550
HLFVTSSFDW TVKLWTTKHN KPLYSFEDNA DYVYDVMWSP VHPALFACVD
560 570 580 590 600
GMGRLDLWNL NSDTEVPTAS VAIEGAYALN RVRWAQGGKE VAVGDSEGRI
610 620 630 640
WIYDVGELAV PHNDEWTRFA RTLVEIRANR ADSEEEGAVE LAA
Length:643
Mass (Da):72,754
Last modified:November 1, 1997 - v1
Checksum:iBD126092A6633402
GO
Isoform 2 (identifier: Q63100-3) [UniParc]FASTAAdd to basket

Also known as: 1B

The sequence of this isoform differs from the canonical sequence as follows:
     75-90: Missing.

Show »
Length:627
Mass (Da):70,702
Checksum:iC86E6231A0B82368
GO
Isoform 3 (identifier: Q63100-4) [UniParc]FASTAAdd to basket

Also known as: 1C

The sequence of this isoform differs from the canonical sequence as follows:
     75-90: Missing.
     121-141: Missing.

Show »
Length:606
Mass (Da):68,331
Checksum:i0D0CFB9525D9C8B8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei75 – 9016Missing in isoform 2 and isoform 3. CuratedVSP_039087Add
BLAST
Alternative sequencei121 – 14121Missing in isoform 3. CuratedVSP_039088Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66845 mRNA. Translation: CAA47321.1.
PIRiA43423.
RefSeqiNP_062107.1. NM_019234.1. [Q63100-1]
UniGeneiRn.11273.

Genome annotation databases

GeneIDi29564.
KEGGirno:29564.
UCSCiRGD:2512. rat. [Q63100-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66845 mRNA. Translation: CAA47321.1.
PIRiA43423.
RefSeqiNP_062107.1. NM_019234.1. [Q63100-1]
UniGeneiRn.11273.

3D structure databases

ProteinModelPortaliQ63100.
SMRiQ63100. Positions 145-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248198. 6 interactions.
IntActiQ63100. 1 interaction.
MINTiMINT-4542819.
STRINGi10116.ENSRNOP00000013184.

PTM databases

iPTMnetiQ63100.
PhosphoSiteiQ63100.
SwissPalmiQ63100.

Proteomic databases

PaxDbiQ63100.
PRIDEiQ63100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29564.
KEGGirno:29564.
UCSCiRGD:2512. rat. [Q63100-1]

Organism-specific databases

CTDi1780.
RGDi2512. Dync1i1.

Phylogenomic databases

eggNOGiKOG1587. Eukaryota.
ENOG410XQ99. LUCA.
HOGENOMiHOG000116383.
HOVERGENiHBG004083.
InParanoidiQ63100.
KOiK10415.
PhylomeDBiQ63100.

Miscellaneous databases

NextBioi609628.
PROiQ63100.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR025956. DYNC1I1/DYNC1I2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF11540. Dynein_IC2. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function."
    Paschal B.M., Mikami A., Pfister K.K., Vallee R.B.
    J. Cell Biol. 118:1133-1143(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex."
    Karki S., Holzbaur E.L.
    J. Biol. Chem. 270:28806-28811(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DCTN1.
  3. "Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued."
    Vaughan K.T., Vallee R.B.
    J. Cell Biol. 131:1507-1516(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DCTN1.
  4. "Identification and developmental regulation of a neuron-specific subunit of cytoplasmic dynein."
    Pfister K.K., Salata M.W., Dillman J.F. III, Torre E., Lye R.J.
    Mol. Biol. Cell 7:331-343(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CYTOPLASMIC DYNEIN 1 COMPLEX.
  5. "Cytoplasmic dynein contains a family of differentially expressed light chains."
    King S.M., Barbarese E., Dillman J.F. III, Benashski S.E., Do K.T., Patel-King R.S., Pfister K.K.
    Biochemistry 37:15033-15041(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CYTOPLASMIC DYNEIN 1 COMPLEX.
  6. "Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex."
    Lo K.W., Kogoy J.M., Rasoul B.A., King S.M., Pfister K.K.
    J. Biol. Chem. 282:36871-36878(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNLT1 AND DYNLT3.
  7. "Intermediate chain subunit as a probe for cytoplasmic dynein function: biochemical analyses and live cell imaging in PC12 cells."
    Myers K.R., Lo K.W., Lye R.J., Kogoy J.M., Soura V., Hafezparast M., Pfister K.K.
    J. Neurosci. Res. 85:2640-2647(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), INTERACTION WITH DYNC1H1.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111; SER-177; SER-195 AND SER-633, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDC1I1_RAT
AccessioniPrimary (citable) accession number: Q63100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 17, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.