Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ALX homeobox protein 1

Gene

Alx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes (PubMed:12929931). Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures (By similarity). Early on in development, it plays a role in forebrain mesenchyme survival (By similarity). May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi132 – 19160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ALX homeobox protein 1Curated
Alternative name(s):
Cartilage homeoprotein 11 Publication
Short name:
CART-11 Publication
Gene namesi
Name:Alx1Imported
Synonyms:Cart1By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2273. Alx1.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: UniProtKB
  • transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1311K → R: Loss of EP300-mediated transcriptional coactivation. 1 Publication
Mutagenesisi133 – 1364RRHR → GGHG: Loss of transcriptional activity. Loss of DNA-binding. Altered localization to the nucleus. 1 Publication
Mutagenesisi188 – 1903RKR → GKG: Loss of transcriptional activity. No effect on DNA-binding. Loss of homodimerization. Altered localization to the nucleus. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326ALX homeobox protein 1PRO_0000048857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei131 – 1311N6-acetyllysine; by EP300By similarity

Post-translational modificationi

Acetylated at Lys-131 by EP300; increases interaction with EP300 and stimulates ALX1 transcriptional activity.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ63087.
PRIDEiQ63087.

PTM databases

iPTMnetiQ63087.
PhosphoSiteiQ63087.

Expressioni

Tissue specificityi

Expressed in chondrocytes.1 Publication

Developmental stagei

Expressed in condensed prechondrocytic mesenchymal cells and in early chondrocytes of cartilage primordia. Expression is lower in mature chondrocytes.1 Publication

Gene expression databases

ExpressionAtlasiQ63087. baseline and differential.
GenevisibleiQ63087. RN.

Interactioni

Subunit structurei

Binds DNA as a homodimer; required for transcriptional activation (PubMed:12390248). Interacts (via homeobox domain) with EP300; acetylates ALX1 and stimulates its transcriptional activity (PubMed:12929931).2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006007.

Structurei

3D structure databases

ProteinModelPortaliQ63087.
SMRiQ63087. Positions 131-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 326135Transactivation domain1 PublicationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi306 – 31914OARPROSITE-ProRule annotationAdd
BLAST

Domaini

The OAR motif may negatively regulate DNA-binding and therefore transcriptional activity. It is found in the C-terminal transactivation domain that stimulates transcription.2 Publications

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG050798.
InParanoidiQ63087.
KOiK09334.
OMAiHVSLNNF.
OrthoDBiEOG7C2R1F.
PhylomeDBiQ63087.
TreeFamiTF350743.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR033209. ALX1.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PANTHERiPTHR24329:SF359. PTHR24329:SF359. 2 hits.
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63087-1) [UniParc]FASTAAdd to basket

Also known as: alpha1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEFLSEKFAL KSPPSKNSDF YMGTGGALEH VMETLDNESF YGKATAGKCV
60 70 80 90 100
QAFGPLPRAE HHVRLDRTSP CQDSSVNYGI TKVEGQPLHT ELNRAMDGCN
110 120 130 140 150
NLRMSPVKGM PEKSELDELG DKCDSNVSSS KKRRHRTTFT SLQLEELEKV
160 170 180 190 200
FQKTHYPDVY VREQLALRTE LTEARVQVWF QNRRAKWRKR ERYGQIQQAK
210 220 230 240 250
SHFAATYDIS VLPRTDSYPQ IQNNLWAGNT SGGSVVTSCM LPRDASSCMT
260 270 280 290 300
PYSHSPRTDS SYTGFSNHQN QFGHVPLNNF FTDSLLTGTT NGHAFETKPE
310 320
FERRSSSIAV LRMKAKEHTA NISWAM
Length:326
Mass (Da):36,904
Last modified:November 1, 1996 - v1
Checksum:i0DD201EC595C74AE
GO
Isoform 2 (identifier: Q63087-2) [UniParc]FASTAAdd to basket

Also known as: beta1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     178-192: VWFQNRRAKWRKRER → GQVEKKRTLRPNTAS
     193-326: Missing.

Note: Unable to bind DNA and activate transcription. Acts as a dominant negative through dimerization with isoform 1.1 Publication
Show »
Length:192
Mass (Da):21,652
Checksum:iF71AA11676A9A68C
GO
Isoform 3 (identifier: Q63087-3) [UniParc]FASTAAdd to basket

Also known as: gamma1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     177-219: Missing.

Note: Unable to bind DNA and activate transcription. Acts as a dominant negative through dimerization with isoform 1.1 Publication
Show »
Length:283
Mass (Da):31,638
Checksum:i22A264B960401ED0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei177 – 21943Missing in isoform 3. 1 PublicationVSP_057657Add
BLAST
Alternative sequencei178 – 19215VWFQN…RKRER → GQVEKKRTLRPNTAS in isoform 2. 1 PublicationVSP_057658Add
BLAST
Alternative sequencei193 – 326134Missing in isoform 2. 1 PublicationVSP_057659Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14018 mRNA. Translation: AAA40877.1.
PIRiA47523.
RefSeqiNP_037053.1. NM_012921.1. [Q63087-1]
XP_006241355.1. XM_006241293.2. [Q63087-1]
UniGeneiRn.9282.

Genome annotation databases

EnsembliENSRNOT00000006007; ENSRNOP00000006007; ENSRNOG00000004390. [Q63087-1]
GeneIDi25401.
KEGGirno:25401.
UCSCiRGD:2273. rat. [Q63087-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14018 mRNA. Translation: AAA40877.1.
PIRiA47523.
RefSeqiNP_037053.1. NM_012921.1. [Q63087-1]
XP_006241355.1. XM_006241293.2. [Q63087-1]
UniGeneiRn.9282.

3D structure databases

ProteinModelPortaliQ63087.
SMRiQ63087. Positions 131-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006007.

PTM databases

iPTMnetiQ63087.
PhosphoSiteiQ63087.

Proteomic databases

PaxDbiQ63087.
PRIDEiQ63087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006007; ENSRNOP00000006007; ENSRNOG00000004390. [Q63087-1]
GeneIDi25401.
KEGGirno:25401.
UCSCiRGD:2273. rat. [Q63087-1]

Organism-specific databases

CTDi8092.
RGDi2273. Alx1.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118958.
HOGENOMiHOG000231518.
HOVERGENiHBG050798.
InParanoidiQ63087.
KOiK09334.
OMAiHVSLNNF.
OrthoDBiEOG7C2R1F.
PhylomeDBiQ63087.
TreeFamiTF350743.

Miscellaneous databases

NextBioi606493.
PROiQ63087.

Gene expression databases

ExpressionAtlasiQ63087. baseline and differential.
GenevisibleiQ63087. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR033209. ALX1.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PANTHERiPTHR24329:SF359. PTHR24329:SF359. 2 hits.
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cartilage homeoprotein 1, a homeoprotein selectively expressed in chondrocytes."
    Zhao G.-Q., Zhou X., Eberspaecher H., Solursh M., de Crombrugghe B.
    Proc. Natl. Acad. Sci. U.S.A. 90:8633-8637(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Chondrosarcoma.
  2. "Functional domains of paired-like homeoprotein Cart1 and the relationship between dimerization and transcription activity."
    Furukawa K., Iioka T., Morishita M., Yamaguchi A., Shindo H., Namba H., Yamashita S., Tsukazaki T.
    Genes Cells 7:1135-1147(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), SUBUNIT, SUBCELLULAR LOCATION, REGION.
  3. "P300/CBP acts as a coactivator to cartilage homeoprotein-1 (Cart1), paired-like homeoprotein, through acetylation of the conserved lysine residue adjacent to the homeodomain."
    Iioka T., Furukawa K., Yamaguchi A., Shindo H., Yamashita S., Tsukazaki T.
    J. Bone Miner. Res. 18:1419-1429(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EP300, SUBCELLULAR LOCATION, ACETYLATION AT LYS-131, MUTAGENESIS OF LYS-131.
  4. "The OAR/aristaless domain of the homeodomain protein Cart1 has an attenuating role in vivo."
    Brouwer A., ten Berge D., Wiegerinck R., Meijlink F.
    Mech. Dev. 120:241-252(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.

Entry informationi

Entry nameiALX1_RAT
AccessioniPrimary (citable) accession number: Q63087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.