Reviewed,
UniProtKB/Swiss-Prot Q63085 (PDE3B_RAT)
Last modified
January 19, 2010.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: cGMP-inhibited 3',5'-cyclic phosphodiesterase B EC=3.1.4.17 Alternative name(s): Cyclic GMP-inhibited phosphodiesterase B Short name=CGI-PDE B CGIPDE1 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1108 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism By similarity. |
| Catalytic activity | Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate. |
| Cofactor | Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity. |
| Enzyme regulation | Inhibited by cGMP. |
| Subcellular location | Membrane; Multi-pass membrane protein Potential. |
| Tissue specificity | Abundant in adipose tissues. |
| Sequence similarities | Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1108 | 1108 | cGMP-inhibited 3',5'-cyclic phosphodiesterase B | PRO_0000198804 | |||||
Regions | |||||||||
| Transmembrane | 73 – 93 | 21 | Potential | ||||||
| Transmembrane | 114 – 134 | 21 | Potential | ||||||
| Transmembrane | 144 – 164 | 21 | Potential | ||||||
| Transmembrane | 175 – 195 | 21 | Potential | ||||||
| Transmembrane | 204 – 224 | 21 | Potential | ||||||
| Transmembrane | 231 – 251 | 21 | Potential | ||||||
| Region | 695 – 1063 | 369 | Catalytic By similarity | ||||||
| Compositional bias | 16 – 22 | 7 | Poly-Pro | ||||||
| Compositional bias | 99 – 102 | 4 | Poly-Ala | ||||||
| Compositional bias | 175 – 179 | 5 | Poly-Ala | ||||||
| Compositional bias | 1007 – 1021 | 15 | Poly-Asp | ||||||
| Compositional bias | 1068 – 1071 | 4 | Poly-Glu | ||||||
| Compositional bias | 1101 – 1104 | 4 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 719 | 1 | Proton donor By similarity | ||||||
| Metal binding | 723 | 1 | Divalent metal cation 1 By similarity | ||||||
| Metal binding | 803 | 1 | Divalent metal cation 1 By similarity | ||||||
| Metal binding | 804 | 1 | Divalent metal cation 1 By similarity | ||||||
| Metal binding | 804 | 1 | Divalent metal cation 2 By similarity | ||||||
| Metal binding | 919 | 1 | Divalent metal cation 1 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 427 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 610 | 1 | Phosphothreonine By similarity | ||||||
Sequences
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References
| [1] | "Molecular cloning of the rat adipocyte hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase." Taira M., Hockman S.C., Calvo J.C., Taira M., Belfrage P., Manganiello V.C. J. Biol. Chem. 268:18573-18579(1993) [PubMed: 8395509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Adipose tissue. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z22867 mRNA. Translation: CAA80489.1. |
| IPI | IPI00951591. |
| PIR | A48508. |
| RefSeq | NP_058925.1. |
| UniGene | Rn.10322 |
3D structure databases | |
| SMR | Q63085. Positions 638-1064. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q63085. |
PTM databases | |
| PhosphoSite | Q63085. |
Proteomic databases | |
| PRIDE | Q63085. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000015498; ENSRNOP00000015498; ENSRNOG00000011417; Rattus norvegicus. [Genome view] |
| GeneID | 29516. |
| KEGG | rno:29516. |
Organism-specific databases | |
| CTD | 29516. |
| RGD | 61943. Pde3b. |
Phylogenomic databases | |
| eggNOG | roNOG10811. |
| HOVERGEN | Q63085. |
| InParanoid | Q63085. |
Enzyme and pathway databases | |
| BRENDA | 3.1.4.17. 248. |
Gene expression databases | |
| ArrayExpress | Q63085. |
| Genevestigator | Q63085. |
Family and domain databases | |
| InterPro | IPR003607. Metal-dep_PHydrolase_HD_dom. IPR002073. PDEase. [Graphical view] |
| Pfam | PF00233. PDEase_I. 1 hit. [Graphical view] |
| SMART | SM00471. HDc. 1 hit. [Graphical view] |
| PROSITE | PS00126. PDEASE_I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 609453. |
Entry information
| Entry name | PDE3B_RAT | ||||||||
| Accession | Primary (citable) accession number: Q63085 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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