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Q63085

- PDE3B_RAT

UniProt

Q63085 - PDE3B_RAT

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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Gene

Pde3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei719 – 7191Proton donorBy similarity
Metal bindingi723 – 7231Divalent metal cation 1By similarity
Metal bindingi803 – 8031Divalent metal cation 1By similarity
Metal bindingi804 – 8041Divalent metal cation 1By similarity
Metal bindingi804 – 8041Divalent metal cation 2By similarity
Metal bindingi919 – 9191Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
  2. cAMP binding Source: RGD
  3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: RGD
  4. drug binding Source: RGD
  5. metal ion binding Source: UniProtKB-KW
  6. phosphoprotein binding Source: RGD
  7. protein kinase B binding Source: BHF-UCL

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cAMP catabolic process Source: RGD
  3. cellular response to insulin stimulus Source: BHF-UCL
  4. diterpenoid metabolic process Source: RGD
  5. negative regulation of angiogenesis Source: UniProtKB
  6. negative regulation of insulin secretion Source: RGD
  7. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Angiogenesis

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Enzyme and pathway databases

SABIO-RKQ63085.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)
Alternative name(s):
CGIPDE1
Cyclic GMP-inhibited phosphodiesterase B
Short name:
CGI-PDE B
Gene namesi
Name:Pde3b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61943. Pde3b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Transmembranei114 – 13421HelicalSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11081108cGMP-inhibited 3',5'-cyclic phosphodiesterase BPRO_0000198804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei279 – 2791Phosphoserine; by PKB/AKT1 or PKB/AKT2By similarity
Modified residuei427 – 4271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63085.
PRIDEiQ63085.

PTM databases

PhosphoSiteiQ63085.

Expressioni

Tissue specificityi

Abundant in adipose tissues.

Gene expression databases

GenevestigatoriQ63085.

Interactioni

Subunit structurei

Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015498.

Structurei

3D structure databases

ProteinModelPortaliQ63085.
SMRiQ63085. Positions 638-1064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3232Interaction with RAPGEF3By similarityAdd
BLAST
Regioni421 – 44525Interaction with PIK3R6By similarityAdd
BLAST
Regioni695 – 1063369CatalyticBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi16 – 227Poly-Pro
Compositional biasi99 – 1024Poly-Ala
Compositional biasi175 – 1795Poly-Ala
Compositional biasi1007 – 102115Poly-AspAdd
BLAST
Compositional biasi1068 – 10714Poly-Glu
Compositional biasi1101 – 11044Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145074.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ63085.
KOiK13296.
PhylomeDBiQ63085.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63085-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKDERERDT PAMRSPPPPP PPATATAASP PESLRNGYVK SCVSPLRQDP
60 70 80 90 100
PRSFFFHLCR FCNVEPPAAS LRAGARLSLA ALAAFVLAAL LGAGPERWAA
110 120 130 140 150
AATGLRTLLS ACSLSLSPLF SIACAFFFLT CFLTRAQRGP DRGAGSWWLL
160 170 180 190 200
ALPACCYLGD FAAWQWWSWL RGEPAAAAAG RLCLVLSCVG LLTLAPRVRL
210 220 230 240 250
RHGVLVLLFA GLVWWVSFSG LGALPPALRP LLSCLVGGAG CLLALGLDHF
260 270 280 290 300
FHVRGASPPP RSASTADEKV PVIRPRRRSS CVSLGESAAG YYGSGKMFRR
310 320 330 340 350
PSLPCISREQ MILWDWDLKQ WCKPHYQNSG GGNGVDLSVL NEARNMVSDL
360 370 380 390 400
LIDPSLPPQV ISSLRSISSL MGAFSGSCRP KINSFTPFPG FYPCSEVEDP
410 420 430 440 450
VEKGDRKLHK GLSSKPSFPT AQLRRSSGAS GLLTSEHHSR WDRSGGKRPY
460 470 480 490 500
QELSVSSHGC HLNGPFSSNL MTIPKQRSSS VSLTHHAGLR RAGALPSPSL
510 520 530 540 550
LNSSSHVPVS AGCLTNRSPV GFLDTSDFLT KPSVTLHRSL GSVSSAADFH
560 570 580 590 600
QYLRNSDSSL CSSCGHQILK YVSTCEPDGT DHHNEKSGEE DSTVFSKERL
610 620 630 640 650
NIVETQEEET VKEDCRELFL EGDDHLMEEA QQPNIDQEVL LDPMLVEDYD
660 670 680 690 700
SLIEKMSNWN FQIFELVEKM GEKSGRILSQ VMYTLFQDTG LLETFKIPTQ
710 720 730 740 750
EFMNYFRALE NGYRDIPYHN RVHATDVLHA VWYLTTRPIP GLQQLHNNHE
760 770 780 790 800
TETKADSDAR LSSGQIAYLS SKSCCIPDKS YGCLSSNIPA LELMALYVAA
810 820 830 840 850
AMHDYDHPGR TNAFLVATNA PQAVLYNDRS VLENHHAASA WNLYLSRPEY
860 870 880 890 900
NFLLNLDHME FKRFRFLVIE AILATDLKKH FDFLAEFNAK ANDVNSNGIE
910 920 930 940 950
WSSENDRLLV CQVCIKLADI NGPAKDRDLH LRWTEGIVNE FYEQGDEEAT
960 970 980 990 1000
LGLPISPFMD RSSPQLAKLQ ESFITHIVGP LCNSYDAAGL LPGQWIEAEE
1010 1020 1030 1040 1050
GDDTESDDDD DDDDDDDDDD DEELDSDDEE TEDNLNPKPQ RRKGRRRIFC
1060 1070 1080 1090 1100
QLMHHLTENH KIWKEIIEEE EKCKAEGNKL QVDNASLPQA DEIQVIEEAD

EEEEQMFE
Length:1,108
Mass (Da):123,107
Last modified:November 1, 1996 - v1
Checksum:iC9B5078C7D3ADD6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22867 mRNA. Translation: CAA80489.1.
PIRiA48508.
RefSeqiNP_058925.1. NM_017229.1.
UniGeneiRn.10322.

Genome annotation databases

GeneIDi29516.
KEGGirno:29516.
UCSCiRGD:61943. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22867 mRNA. Translation: CAA80489.1 .
PIRi A48508.
RefSeqi NP_058925.1. NM_017229.1.
UniGenei Rn.10322.

3D structure databases

ProteinModelPortali Q63085.
SMRi Q63085. Positions 638-1064.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015498.

PTM databases

PhosphoSitei Q63085.

Proteomic databases

PaxDbi Q63085.
PRIDEi Q63085.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29516.
KEGGi rno:29516.
UCSCi RGD:61943. rat.

Organism-specific databases

CTDi 5140.
RGDi 61943. Pde3b.

Phylogenomic databases

eggNOGi NOG145074.
HOGENOMi HOG000060144.
HOVERGENi HBG053541.
InParanoidi Q63085.
KOi K13296.
PhylomeDBi Q63085.

Enzyme and pathway databases

SABIO-RK Q63085.

Miscellaneous databases

NextBioi 609453.
PROi Q63085.

Gene expression databases

Genevestigatori Q63085.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the rat adipocyte hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase."
    Taira M., Hockman S.C., Calvo J.C., Taira M., Belfrage P., Manganiello V.C.
    J. Biol. Chem. 268:18573-18579(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Adipose tissue.

Entry informationi

Entry nameiPDE3B_RAT
AccessioniPrimary (citable) accession number: Q63085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3