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Q63085

- PDE3B_RAT

UniProt

Q63085 - PDE3B_RAT

Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Gene

Pde3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis By similarity.By similarity

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by cGMP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei719 – 7191Proton donorBy similarity
    Metal bindingi723 – 7231Divalent metal cation 1By similarity
    Metal bindingi803 – 8031Divalent metal cation 1By similarity
    Metal bindingi804 – 8041Divalent metal cation 1By similarity
    Metal bindingi804 – 8041Divalent metal cation 2By similarity
    Metal bindingi919 – 9191Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
    2. cAMP binding Source: RGD
    3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: RGD
    4. drug binding Source: RGD
    5. metal ion binding Source: UniProtKB-KW
    6. phosphoprotein binding Source: RGD
    7. protein kinase B binding Source: BHF-UCL

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cAMP catabolic process Source: RGD
    3. cellular response to insulin stimulus Source: BHF-UCL
    4. diterpenoid metabolic process Source: RGD
    5. negative regulation of angiogenesis Source: UniProtKB
    6. negative regulation of insulin secretion Source: RGD
    7. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Angiogenesis

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    SABIO-RKQ63085.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC:3.1.4.17)
    Alternative name(s):
    CGIPDE1
    Cyclic GMP-inhibited phosphodiesterase B
    Short name:
    CGI-PDE B
    Gene namesi
    Name:Pde3b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61943. Pde3b.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11081108cGMP-inhibited 3',5'-cyclic phosphodiesterase BPRO_0000198804Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei279 – 2791Phosphoserine; by PKB/AKT1 or PKB/AKT2By similarity
    Modified residuei427 – 4271PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ63085.
    PRIDEiQ63085.

    PTM databases

    PhosphoSiteiQ63085.

    Expressioni

    Tissue specificityi

    Abundant in adipose tissues.

    Gene expression databases

    GenevestigatoriQ63085.

    Interactioni

    Subunit structurei

    Interacts with PIK3CG. Interacts with RAPGEF3 and PIK3R6 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63085.
    SMRiQ63085. Positions 638-1064.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei73 – 9321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei114 – 13421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei204 – 22421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3232Interaction with RAPGEF3By similarityAdd
    BLAST
    Regioni421 – 44525Interaction with PIK3R6By similarityAdd
    BLAST
    Regioni695 – 1063369CatalyticBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi16 – 227Poly-Pro
    Compositional biasi99 – 1024Poly-Ala
    Compositional biasi175 – 1795Poly-Ala
    Compositional biasi1007 – 102115Poly-AspAdd
    BLAST
    Compositional biasi1068 – 10714Poly-Glu
    Compositional biasi1101 – 11044Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ63085.
    KOiK13296.
    PhylomeDBiQ63085.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63085-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKDERERDT PAMRSPPPPP PPATATAASP PESLRNGYVK SCVSPLRQDP     50
    PRSFFFHLCR FCNVEPPAAS LRAGARLSLA ALAAFVLAAL LGAGPERWAA 100
    AATGLRTLLS ACSLSLSPLF SIACAFFFLT CFLTRAQRGP DRGAGSWWLL 150
    ALPACCYLGD FAAWQWWSWL RGEPAAAAAG RLCLVLSCVG LLTLAPRVRL 200
    RHGVLVLLFA GLVWWVSFSG LGALPPALRP LLSCLVGGAG CLLALGLDHF 250
    FHVRGASPPP RSASTADEKV PVIRPRRRSS CVSLGESAAG YYGSGKMFRR 300
    PSLPCISREQ MILWDWDLKQ WCKPHYQNSG GGNGVDLSVL NEARNMVSDL 350
    LIDPSLPPQV ISSLRSISSL MGAFSGSCRP KINSFTPFPG FYPCSEVEDP 400
    VEKGDRKLHK GLSSKPSFPT AQLRRSSGAS GLLTSEHHSR WDRSGGKRPY 450
    QELSVSSHGC HLNGPFSSNL MTIPKQRSSS VSLTHHAGLR RAGALPSPSL 500
    LNSSSHVPVS AGCLTNRSPV GFLDTSDFLT KPSVTLHRSL GSVSSAADFH 550
    QYLRNSDSSL CSSCGHQILK YVSTCEPDGT DHHNEKSGEE DSTVFSKERL 600
    NIVETQEEET VKEDCRELFL EGDDHLMEEA QQPNIDQEVL LDPMLVEDYD 650
    SLIEKMSNWN FQIFELVEKM GEKSGRILSQ VMYTLFQDTG LLETFKIPTQ 700
    EFMNYFRALE NGYRDIPYHN RVHATDVLHA VWYLTTRPIP GLQQLHNNHE 750
    TETKADSDAR LSSGQIAYLS SKSCCIPDKS YGCLSSNIPA LELMALYVAA 800
    AMHDYDHPGR TNAFLVATNA PQAVLYNDRS VLENHHAASA WNLYLSRPEY 850
    NFLLNLDHME FKRFRFLVIE AILATDLKKH FDFLAEFNAK ANDVNSNGIE 900
    WSSENDRLLV CQVCIKLADI NGPAKDRDLH LRWTEGIVNE FYEQGDEEAT 950
    LGLPISPFMD RSSPQLAKLQ ESFITHIVGP LCNSYDAAGL LPGQWIEAEE 1000
    GDDTESDDDD DDDDDDDDDD DEELDSDDEE TEDNLNPKPQ RRKGRRRIFC 1050
    QLMHHLTENH KIWKEIIEEE EKCKAEGNKL QVDNASLPQA DEIQVIEEAD 1100
    EEEEQMFE 1108
    Length:1,108
    Mass (Da):123,107
    Last modified:November 1, 1996 - v1
    Checksum:iC9B5078C7D3ADD6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22867 mRNA. Translation: CAA80489.1.
    PIRiA48508.
    RefSeqiNP_058925.1. NM_017229.1.
    UniGeneiRn.10322.

    Genome annotation databases

    GeneIDi29516.
    KEGGirno:29516.
    UCSCiRGD:61943. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22867 mRNA. Translation: CAA80489.1 .
    PIRi A48508.
    RefSeqi NP_058925.1. NM_017229.1.
    UniGenei Rn.10322.

    3D structure databases

    ProteinModelPortali Q63085.
    SMRi Q63085. Positions 638-1064.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015498.

    Chemistry

    BindingDBi Q63085.

    PTM databases

    PhosphoSitei Q63085.

    Proteomic databases

    PaxDbi Q63085.
    PRIDEi Q63085.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29516.
    KEGGi rno:29516.
    UCSCi RGD:61943. rat.

    Organism-specific databases

    CTDi 5140.
    RGDi 61943. Pde3b.

    Phylogenomic databases

    eggNOGi NOG145074.
    HOGENOMi HOG000060144.
    HOVERGENi HBG053541.
    InParanoidi Q63085.
    KOi K13296.
    PhylomeDBi Q63085.

    Enzyme and pathway databases

    SABIO-RK Q63085.

    Miscellaneous databases

    NextBioi 609453.
    PROi Q63085.

    Gene expression databases

    Genevestigatori Q63085.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the rat adipocyte hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase."
      Taira M., Hockman S.C., Calvo J.C., Taira M., Belfrage P., Manganiello V.C.
      J. Biol. Chem. 268:18573-18579(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Adipose tissue.

    Entry informationi

    Entry nameiPDE3B_RAT
    AccessioniPrimary (citable) accession number: Q63085
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3