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Protein

Nucleobindin-1

Gene

Nucb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi171 – 21747Sequence analysisAdd
BLAST
Calcium bindingi252 – 263121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi304 – 315122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • DNA binding Source: UniProtKB-KW
  • G-protein alpha-subunit binding Source: RGD

GO - Biological processi

  • regulation of protein targeting Source: RGD
  • response to cisplatin Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, DNA-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-1
Alternative name(s):
Bone 63 kDa calcium-binding protein
CALNUC
Gene namesi
Name:Nucb1
Synonyms:Nuc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620030. Nucb1.

Subcellular locationi

GO - Cellular componenti

  • cis-Golgi network Source: RGD
  • cytoplasm Source: RGD
  • early endosome Source: RGD
  • endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular region Source: RGD
  • extracellular space Source: RGD
  • extrinsic component of Golgi membrane Source: RGD
  • Golgi apparatus Source: RGD
  • Golgi-associated vesicle Source: RGD
  • Golgi cisterna membrane Source: RGD
  • lumenal side of Golgi membrane Source: RGD
  • microtubule cytoskeleton Source: Ensembl
  • nucleus Source: RGD
  • rough endoplasmic reticulum Source: RGD
  • trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 459434Nucleobindin-1PRO_0000004164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851PhosphoserineCombined sources
Modified residuei147 – 1471PhosphothreonineBy similarity
Modified residuei368 – 3681PhosphoserineCombined sources
Modified residuei456 – 4561PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63083.
PRIDEiQ63083.

PTM databases

iPTMnetiQ63083.
PhosphoSiteiQ63083.

Expressioni

Tissue specificityi

Localized in the soft tissue in the center of the osteon and in the osteocyte lacuna. Minor constituent of the mineralized matrix of bone. Detected in calvaria, rib cartilage, liver, kidney, spleen, brain, lung, skeletal and heart muscle.

Gene expression databases

BgeeiENSRNOG00000020889.
GenevisibleiQ63083. RN.

Interactioni

Subunit structurei

Interacts with GNAI3 and binds necdin.1 Publication

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: RGD

Protein-protein interaction databases

BioGridi250025. 2 interactions.
IntActiQ63083. 1 interaction.
MINTiMINT-4565910.
STRINGi10116.ENSRNOP00000028390.

Structurei

3D structure databases

ProteinModelPortaliQ63083.
SMRiQ63083. Positions 227-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini239 – 27436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini291 – 32636EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 32094Binds to GNAI2 and GNAI3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili149 – 21769Sequence analysisAdd
BLAST
Coiled coili340 – 40768Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi400 – 4056Poly-Gln

Sequence similaritiesi

Belongs to the nucleobindin family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ63083.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ63083.
TreeFamiTF323218.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTSVPRGAP FLLLPPLLML SAVLAVPVDR AAPHQEDNQA TETPDTGLYY
60 70 80 90 100
HRYLQEVINV LETDGHFREK LQAANAEDIK SGKLSQELDF VSHNVRTKLD
110 120 130 140 150
ELKRQEVSRL RMLLKAKMDA KQEPNLQVDH MNLLKQFEHL DPQNQHTFEA
160 170 180 190 200
RDLELLIQTA TRDLAQYDAA HHEEFKRYEM LKEHERRRYL ESLGEEQRKE
210 220 230 240 250
AERKLQEQQR RHREHPKVNV PGSQAQLKEV WEELDGLDPN RFNPKTFFIL
260 270 280 290 300
HDINSDGVLD EQELEALFTK ELEKVYDPKN EEDDMREMEE ERLRMREHVM
310 320 330 340 350
KNVDTNQDRL VTLEEFLAST QRKEFGETAE GWKTVEMYPA YTEEELKRFE
360 370 380 390 400
EELAAREAEL NARAQRLSQE TEALGRSQDR LEAQKRELQQ AVLQMEQRKQ
410 420 430 440 450
QQQEQSAPPS QPDGQLQFRA DTGDAPVPAP AGDQKDVPAS EKKVPEQPPV

LPQLDSQHL
Length:459
Mass (Da):53,507
Last modified:November 1, 1997 - v1
Checksum:i862BC82673002949
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36277 mRNA. Translation: CAA85285.1.
U75409 mRNA. Translation: AAB19111.1.
BC100643 mRNA. Translation: AAI00644.1.
AF336828 mRNA. Translation: AAK21297.1.
PIRiI55472.
RefSeqiNP_445915.1. NM_053463.1.
XP_006229227.1. XM_006229165.1.
UniGeneiRn.1492.

Genome annotation databases

EnsembliENSRNOT00000028390; ENSRNOP00000028390; ENSRNOG00000020889.
GeneIDi84595.
KEGGirno:84595.
UCSCiRGD:620030. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36277 mRNA. Translation: CAA85285.1.
U75409 mRNA. Translation: AAB19111.1.
BC100643 mRNA. Translation: AAI00644.1.
AF336828 mRNA. Translation: AAK21297.1.
PIRiI55472.
RefSeqiNP_445915.1. NM_053463.1.
XP_006229227.1. XM_006229165.1.
UniGeneiRn.1492.

3D structure databases

ProteinModelPortaliQ63083.
SMRiQ63083. Positions 227-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250025. 2 interactions.
IntActiQ63083. 1 interaction.
MINTiMINT-4565910.
STRINGi10116.ENSRNOP00000028390.

PTM databases

iPTMnetiQ63083.
PhosphoSiteiQ63083.

Proteomic databases

PaxDbiQ63083.
PRIDEiQ63083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028390; ENSRNOP00000028390; ENSRNOG00000020889.
GeneIDi84595.
KEGGirno:84595.
UCSCiRGD:620030. rat.

Organism-specific databases

CTDi4924.
RGDi620030. Nucb1.

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
GeneTreeiENSGT00390000001927.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiQ63083.
OMAiAKMDAEQ.
OrthoDBiEOG091G0HG8.
PhylomeDBiQ63083.
TreeFamiTF323218.

Miscellaneous databases

PROiQ63083.

Gene expression databases

BgeeiENSRNOG00000020889.
GenevisibleiQ63083. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCB1_RAT
AccessioniPrimary (citable) accession number: Q63083
Secondary accession number(s): P97623, Q497A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Discovered as DNA-binding protein in the serum of lupus-prone mice.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.