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Q63081

- PDIA6_RAT

UniProt

Q63081 - PDIA6_RAT

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Protein
Protein disulfide-isomerase A6
Gene
Pdia6, Cabp1, Txndc7
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin By similarity.

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551Nucleophile By similarity
Sitei56 – 561Contributes to redox potential value By similarity
Sitei57 – 571Contributes to redox potential value By similarity
Active sitei58 – 581Nucleophile By similarity
Sitei118 – 1181Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei190 – 1901Nucleophile By similarity
Sitei191 – 1911Contributes to redox potential value By similarity
Sitei192 – 1921Contributes to redox potential value By similarity
Active sitei193 – 1931Nucleophile By similarity
Sitei256 – 2561Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. calcium ion binding Source: RGD
  2. protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic cell clearance Source: RefGenome
  2. cell redox homeostasis Source: InterPro
  3. platelet activation Source: UniProtKB
  4. platelet aggregation Source: UniProtKB
  5. protein folding Source: RefGenome
  6. response to endoplasmic reticulum stress Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A6 (EC:5.3.4.1)
Alternative name(s):
Calcium-binding protein 1
Short name:
CaBP1
Protein disulfide isomerase P5
Thioredoxin domain-containing protein 7
Gene namesi
Name:Pdia6
Synonyms:Cabp1, Txndc7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi628688. Pdia6.

Subcellular locationi

Endoplasmic reticulum lumen By similarity. Cell membrane By similarity. Melanosome By similarity

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: HGNC
  2. endoplasmic reticulum-Golgi intermediate compartment Source: RGD
  3. melanosome Source: UniProtKB
  4. plasma membrane Source: UniProtKB
  5. smooth endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 By similarity
Add
BLAST
Chaini20 – 440421Protein disulfide-isomerase A6
PRO_0000034240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 58Redox-active By similarity
Disulfide bondi190 ↔ 193Redox-active By similarity
Modified residuei428 – 4281Phosphoserine By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiQ63081.

PTM databases

PhosphoSiteiQ63081.

Expressioni

Gene expression databases

GenevestigatoriQ63081.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with ITGB3 following platelet stimulation By similarity.

Protein-protein interaction databases

BioGridi251898. 1 interaction.
IntActiQ63081. 5 interactions.
MINTiMINT-1775443.

Structurei

3D structure databases

ProteinModelPortaliQ63081.
SMRiQ63081. Positions 20-132, 154-280.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 133113Thioredoxin 1
Add
BLAST
Domaini151 – 287137Thioredoxin 2
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi437 – 4404Prevents secretion from ER By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi422 – 43615Asp/Glu-rich (acidic)
Add
BLAST

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00730000110455.
HOVERGENiHBG053548.
KOiK09584.
OrthoDBiEOG7XDBFV.
PhylomeDBiQ63081.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63081-1 [UniParc]FASTAAdd to Basket

« Hide

MARLVLGLVS CTFFLAVSAL YSSSDDVIEL TPSNFNREVI QSDSLWLVEF    50
YAPWCGHCQR LTPEWKKAAS ALKDVVKVGA VNADKHQSLG GQYGVQGFPT 100
IKIFGANKNK PEDYQGGRTG EAIVDAALSA LRQLVKDRLG GRSGGYSSGK 150
QGRGDSSSKK DVVELTDDTF DKNVLDSEDV WMVEFYAPWC GHCKNLEPEW 200
AAAATEVKEQ TKGKVKLAAV DATVNQVLAS RYGIKGFPTI KIFQKGESPV 250
DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKKT CEEHQLCVVA 300
VLPHILDTGA TGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQYELENALG 350
IGGFGYPAMA AINARKMKFA LLKGSFSEQG INEFLRELSF GRGSTAPVGG 400
GSFPNITPRE PWDGKDGELP VEDDIDLSDV ELDDLEKDEL 440
Length:440
Mass (Da):48,173
Last modified:March 29, 2005 - v2
Checksum:i8D388E4FFD743719
GO

Sequence cautioni

The sequence AAH82063.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082063 mRNA. Translation: AAH82063.1. Different initiation.
X79328 mRNA. Translation: CAA55891.1.
PIRiS45038.
RefSeqiNP_001004442.1. NM_001004442.1.
UniGeneiRn.2685.

Genome annotation databases

EnsembliENSRNOT00000074829; ENSRNOP00000064632; ENSRNOG00000050197.
GeneIDi286906.
KEGGirno:286906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC082063 mRNA. Translation: AAH82063.1 . Different initiation.
X79328 mRNA. Translation: CAA55891.1 .
PIRi S45038.
RefSeqi NP_001004442.1. NM_001004442.1.
UniGenei Rn.2685.

3D structure databases

ProteinModelPortali Q63081.
SMRi Q63081. Positions 20-132, 154-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 251898. 1 interaction.
IntActi Q63081. 5 interactions.
MINTi MINT-1775443.

PTM databases

PhosphoSitei Q63081.

Proteomic databases

PRIDEi Q63081.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000074829 ; ENSRNOP00000064632 ; ENSRNOG00000050197 .
GeneIDi 286906.
KEGGi rno:286906.

Organism-specific databases

CTDi 10130.
RGDi 628688. Pdia6.

Phylogenomic databases

GeneTreei ENSGT00730000110455.
HOVERGENi HBG053548.
KOi K09584.
OrthoDBi EOG7XDBFV.
PhylomeDBi Q63081.

Miscellaneous databases

NextBioi 625007.

Gene expression databases

Genevestigatori Q63081.

Family and domain databases

Gene3Di 3.40.30.10. 2 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 3 hits.
TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "CaBP1, a calcium binding protein of the thioredoxin family, is a resident KDEL protein of the ER and not of the intermediate compartment."
    Fuellekrug J., Soennichsen B., Wuensch U., Arseven K., Van P.N., Soeling H.-D., Mieskes G.
    J. Cell Sci. 107:2719-2727(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-440.
    Strain: Wistar.
    Tissue: Liver.
  3. Lubec G., Afjehi-Sadat L., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 119-132; 217-231 AND 314-322, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Spinal cord.

Entry informationi

Entry nameiPDIA6_RAT
AccessioniPrimary (citable) accession number: Q63081
Secondary accession number(s): Q641Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: September 3, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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