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Protein

Protein disulfide-isomerase A6

Gene

Pdia6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (By similarity).By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55NucleophileBy similarity1
Sitei56Contributes to redox potential valueBy similarity1
Sitei57Contributes to redox potential valueBy similarity1
Active sitei58NucleophileBy similarity1
Sitei118Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei190NucleophileBy similarity1
Sitei191Contributes to redox potential valueBy similarity1
Sitei192Contributes to redox potential valueBy similarity1
Active sitei193NucleophileBy similarity1
Sitei256Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A6 (EC:5.3.4.1)
Alternative name(s):
Calcium-binding protein 1
Short name:
CaBP1
Protein disulfide isomerase P5
Thioredoxin domain-containing protein 7
Gene namesi
Name:Pdia6
Synonyms:Cabp1, Txndc7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628688. Pdia6.

Subcellular locationi

  • Endoplasmic reticulum lumen By similarity
  • Cell membrane By similarity
  • Melanosome By similarity

GO - Cellular componenti

  • endoplasmic reticulum-Golgi intermediate compartment Source: RGD
  • endoplasmic reticulum lumen Source: HGNC
  • melanosome Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • smooth endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000003424020 – 440Protein disulfide-isomerase A6Add BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 58Redox-activePROSITE-ProRule annotation
Modified residuei129PhosphoserineCombined sources1
Modified residuei156PhosphoserineBy similarity1
Modified residuei158PhosphoserineBy similarity1
Disulfide bondi190 ↔ 193Redox-activePROSITE-ProRule annotation
Modified residuei428PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ63081.
PRIDEiQ63081.

PTM databases

iPTMnetiQ63081.
PhosphoSitePlusiQ63081.

Expressioni

Gene expression databases

BgeeiENSRNOG00000050197.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with ITGB3 following platelet stimulation (By similarity).By similarity

Protein-protein interaction databases

BioGridi251898. 1 interactor.
IntActiQ63081. 5 interactors.
MINTiMINT-1775443.
STRINGi10116.ENSRNOP00000064632.

Structurei

3D structure databases

ProteinModelPortaliQ63081.
SMRiQ63081.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 133Thioredoxin 1PROSITE-ProRule annotationAdd BLAST113
Domaini151 – 287Thioredoxin 2PROSITE-ProRule annotationAdd BLAST137

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi437 – 440Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi422 – 436Asp/Glu-rich (acidic)Add BLAST15

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOVERGENiHBG053548.
InParanoidiQ63081.
KOiK09584.
PhylomeDBiQ63081.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLVLGLVS CTFFLAVSAL YSSSDDVIEL TPSNFNREVI QSDSLWLVEF
60 70 80 90 100
YAPWCGHCQR LTPEWKKAAS ALKDVVKVGA VNADKHQSLG GQYGVQGFPT
110 120 130 140 150
IKIFGANKNK PEDYQGGRTG EAIVDAALSA LRQLVKDRLG GRSGGYSSGK
160 170 180 190 200
QGRGDSSSKK DVVELTDDTF DKNVLDSEDV WMVEFYAPWC GHCKNLEPEW
210 220 230 240 250
AAAATEVKEQ TKGKVKLAAV DATVNQVLAS RYGIKGFPTI KIFQKGESPV
260 270 280 290 300
DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKKT CEEHQLCVVA
310 320 330 340 350
VLPHILDTGA TGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQYELENALG
360 370 380 390 400
IGGFGYPAMA AINARKMKFA LLKGSFSEQG INEFLRELSF GRGSTAPVGG
410 420 430 440
GSFPNITPRE PWDGKDGELP VEDDIDLSDV ELDDLEKDEL
Length:440
Mass (Da):48,173
Last modified:March 29, 2005 - v2
Checksum:i8D388E4FFD743719
GO

Sequence cautioni

The sequence AAH82063 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082063 mRNA. Translation: AAH82063.1. Different initiation.
X79328 mRNA. Translation: CAA55891.1.
PIRiS45038.
RefSeqiNP_001004442.1. NM_001004442.1.
UniGeneiRn.2685.

Genome annotation databases

GeneIDi286906.
KEGGirno:286906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082063 mRNA. Translation: AAH82063.1. Different initiation.
X79328 mRNA. Translation: CAA55891.1.
PIRiS45038.
RefSeqiNP_001004442.1. NM_001004442.1.
UniGeneiRn.2685.

3D structure databases

ProteinModelPortaliQ63081.
SMRiQ63081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251898. 1 interactor.
IntActiQ63081. 5 interactors.
MINTiMINT-1775443.
STRINGi10116.ENSRNOP00000064632.

PTM databases

iPTMnetiQ63081.
PhosphoSitePlusiQ63081.

Proteomic databases

PaxDbiQ63081.
PRIDEiQ63081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi286906.
KEGGirno:286906.

Organism-specific databases

CTDi10130.
RGDi628688. Pdia6.

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOVERGENiHBG053548.
InParanoidiQ63081.
KOiK09584.
PhylomeDBiQ63081.

Miscellaneous databases

PROiQ63081.

Gene expression databases

BgeeiENSRNOG00000050197.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA6_RAT
AccessioniPrimary (citable) accession number: Q63081
Secondary accession number(s): Q641Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.