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Q63081

- PDIA6_RAT

UniProt

Q63081 - PDIA6_RAT

Protein

Protein disulfide-isomerase A6

Gene

Pdia6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin By similarity.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei55 – 551NucleophileBy similarity
    Sitei56 – 561Contributes to redox potential valueBy similarity
    Sitei57 – 571Contributes to redox potential valueBy similarity
    Active sitei58 – 581NucleophileBy similarity
    Sitei118 – 1181Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei190 – 1901NucleophileBy similarity
    Sitei191 – 1911Contributes to redox potential valueBy similarity
    Sitei192 – 1921Contributes to redox potential valueBy similarity
    Active sitei193 – 1931NucleophileBy similarity
    Sitei256 – 2561Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: RGD
    2. protein disulfide isomerase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic cell clearance Source: RefGenome
    2. cell redox homeostasis Source: InterPro
    3. platelet activation Source: UniProtKB
    4. platelet aggregation Source: UniProtKB
    5. protein folding Source: RefGenome
    6. response to endoplasmic reticulum stress Source: RefGenome

    Keywords - Molecular functioni

    Chaperone, Isomerase

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase A6 (EC:5.3.4.1)
    Alternative name(s):
    Calcium-binding protein 1
    Short name:
    CaBP1
    Protein disulfide isomerase P5
    Thioredoxin domain-containing protein 7
    Gene namesi
    Name:Pdia6
    Synonyms:Cabp1, Txndc7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi628688. Pdia6.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation. Cell membrane By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: RGD
    2. endoplasmic reticulum lumen Source: HGNC
    3. melanosome Source: UniProtKB
    4. plasma membrane Source: UniProtKB
    5. smooth endoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 440421Protein disulfide-isomerase A6PRO_0000034240Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 58Redox-activePROSITE-ProRule annotation
    Disulfide bondi190 ↔ 193Redox-activePROSITE-ProRule annotation
    Modified residuei428 – 4281PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiQ63081.

    PTM databases

    PhosphoSiteiQ63081.

    Expressioni

    Gene expression databases

    GenevestigatoriQ63081.

    Interactioni

    Subunit structurei

    Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with ITGB3 following platelet stimulation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi251898. 1 interaction.
    IntActiQ63081. 5 interactions.
    MINTiMINT-1775443.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63081.
    SMRiQ63081. Positions 20-132, 154-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 133113Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 287137Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi437 – 4404Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi422 – 43615Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    GeneTreeiENSGT00730000110455.
    HOVERGENiHBG053548.
    KOiK09584.
    OrthoDBiEOG7XDBFV.
    PhylomeDBiQ63081.

    Family and domain databases

    Gene3Di3.40.30.10. 2 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 3 hits.
    TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63081-1 [UniParc]FASTAAdd to Basket

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    MARLVLGLVS CTFFLAVSAL YSSSDDVIEL TPSNFNREVI QSDSLWLVEF    50
    YAPWCGHCQR LTPEWKKAAS ALKDVVKVGA VNADKHQSLG GQYGVQGFPT 100
    IKIFGANKNK PEDYQGGRTG EAIVDAALSA LRQLVKDRLG GRSGGYSSGK 150
    QGRGDSSSKK DVVELTDDTF DKNVLDSEDV WMVEFYAPWC GHCKNLEPEW 200
    AAAATEVKEQ TKGKVKLAAV DATVNQVLAS RYGIKGFPTI KIFQKGESPV 250
    DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKKT CEEHQLCVVA 300
    VLPHILDTGA TGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQYELENALG 350
    IGGFGYPAMA AINARKMKFA LLKGSFSEQG INEFLRELSF GRGSTAPVGG 400
    GSFPNITPRE PWDGKDGELP VEDDIDLSDV ELDDLEKDEL 440
    Length:440
    Mass (Da):48,173
    Last modified:March 29, 2005 - v2
    Checksum:i8D388E4FFD743719
    GO

    Sequence cautioni

    The sequence AAH82063.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082063 mRNA. Translation: AAH82063.1. Different initiation.
    X79328 mRNA. Translation: CAA55891.1.
    PIRiS45038.
    RefSeqiNP_001004442.1. NM_001004442.1.
    UniGeneiRn.2685.

    Genome annotation databases

    EnsembliENSRNOT00000074829; ENSRNOP00000064632; ENSRNOG00000050197.
    GeneIDi286906.
    KEGGirno:286906.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082063 mRNA. Translation: AAH82063.1 . Different initiation.
    X79328 mRNA. Translation: CAA55891.1 .
    PIRi S45038.
    RefSeqi NP_001004442.1. NM_001004442.1.
    UniGenei Rn.2685.

    3D structure databases

    ProteinModelPortali Q63081.
    SMRi Q63081. Positions 20-132, 154-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251898. 1 interaction.
    IntActi Q63081. 5 interactions.
    MINTi MINT-1775443.

    PTM databases

    PhosphoSitei Q63081.

    Proteomic databases

    PRIDEi Q63081.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000074829 ; ENSRNOP00000064632 ; ENSRNOG00000050197 .
    GeneIDi 286906.
    KEGGi rno:286906.

    Organism-specific databases

    CTDi 10130.
    RGDi 628688. Pdia6.

    Phylogenomic databases

    GeneTreei ENSGT00730000110455.
    HOVERGENi HBG053548.
    KOi K09584.
    OrthoDBi EOG7XDBFV.
    PhylomeDBi Q63081.

    Miscellaneous databases

    NextBioi 625007.

    Gene expression databases

    Genevestigatori Q63081.

    Family and domain databases

    Gene3Di 3.40.30.10. 2 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 3 hits.
    TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "CaBP1, a calcium binding protein of the thioredoxin family, is a resident KDEL protein of the ER and not of the intermediate compartment."
      Fuellekrug J., Soennichsen B., Wuensch U., Arseven K., Van P.N., Soeling H.-D., Mieskes G.
      J. Cell Sci. 107:2719-2727(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-440.
      Strain: Wistar.
      Tissue: Liver.
    3. Lubec G., Afjehi-Sadat L., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 119-132; 217-231 AND 314-322, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain and Spinal cord.

    Entry informationi

    Entry nameiPDIA6_RAT
    AccessioniPrimary (citable) accession number: Q63081
    Secondary accession number(s): Q641Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3