Reviewed, UniProtKB/Swiss-Prot Q63081 (PDIA6_RAT)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase A6
    EC=5.3.4.1
Alternative name(s):
    Protein disulfide isomerase P5
    Calcium-binding protein 1
      Short name=CaBP1
    Thioredoxin domain-containing protein 7

Gene names

Name:	Pdia6
Synonyms:	Cabp1, Txndc7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity. Melanosome By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Ligand	Calcium
Molecular function	Isomerase
PTM	Disulfide bond Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein folding Ref.2 Traceable author statement. Source: RGD
Cellular component	ER-Golgi intermediate compartment Ref.2 Traceable author statement. Source: RGD endoplasmic reticulum lumen Inferred from direct assay. Source: HGNC melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Ref.2 Traceable author statement. Source: RGD protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 440	421	Protein disulfide-isomerase A6		PRO_0000034240
Regions
	Domain	21 – 133	113	Thioredoxin 1
	Domain	151 – 287	137	Thioredoxin 2
	Motif	437 – 440	4	Prevents secretion from ER By similarity
	Compositional bias	422 – 436	15	Asp/Glu-rich (acidic)
Sites
	Active site	55	1	Nucleophile By similarity
	Active site	58	1	Nucleophile By similarity
	Active site	190	1	Nucleophile By similarity
	Active site	193	1	Nucleophile By similarity
	Site	56	1	Contributes to redox potential value By similarity
	Site	57	1	Contributes to redox potential value By similarity
	Site	118	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	191	1	Contributes to redox potential value By similarity
	Site	192	1	Contributes to redox potential value By similarity
	Site	256	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Modified residue	428	1	Phosphoserine By similarity
	Disulfide bond	55 ↔ 58		Redox-active By similarity
	Disulfide bond	190 ↔ 193		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q63081-1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 8D388E4FFD743719	FASTA	440	48,173
	10 20 30 40 50 60 MARLVLGLVS CTFFLAVSAL YSSSDDVIEL TPSNFNREVI QSDSLWLVEF YAPWCGHCQR 70 80 90 100 110 120 LTPEWKKAAS ALKDVVKVGA VNADKHQSLG GQYGVQGFPT IKIFGANKNK PEDYQGGRTG 130 140 150 160 170 180 EAIVDAALSA LRQLVKDRLG GRSGGYSSGK QGRGDSSSKK DVVELTDDTF DKNVLDSEDV 190 200 210 220 230 240 WMVEFYAPWC GHCKNLEPEW AAAATEVKEQ TKGKVKLAAV DATVNQVLAS RYGIKGFPTI 250 260 270 280 290 300 KIFQKGESPV DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKKT CEEHQLCVVA 310 320 330 340 350 360 VLPHILDTGA TGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQYELENALG IGGFGYPAMA 370 380 390 400 410 420 AINARKMKFA LLKGSFSEQG INEFLRELSF GRGSTAPVGG GSFPNITPRE PWDGKDGELP 430 440 VEDDIDLSDV ELDDLEKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"CaBP1, a calcium binding protein of the thioredoxin family, is a resident KDEL protein of the ER and not of the intermediate compartment." Fuellekrug J., Soennichsen B., Wuensch U., Arseven K., Van P.N., Soeling H.-D., Mieskes G. J. Cell Sci. 107:2719-2727(1994) [PubMed: 7876340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-440. Strain: Wistar. Tissue: Liver.
[3]	Lubec G., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 119-132; 217-231 AND 314-322, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082063 mRNA. Translation: AAH82063.1. Different initiation. X79328 mRNA. Translation: CAA55891.1.
IPI	IPI00365929.
PIR	S45038.
RefSeq	NP_001004442.1.
UniGene	Rn.2685
3D structure databases
SMR	Q63081. Positions 18-263, 154-280.
ModBase	Search...
PTM databases
PhosphoSite	Q63081.
Proteomic databases
PRIDE	Q63081.
Genome annotation databases
GeneID	286906.
KEGG	rno:286906.
UCSC	BC082063. rat.
Organism-specific databases
CTD	286906.
RGD	628688. Pdia6.
Phylogenomic databases
HOVERGEN	Q63081.
Enzyme and pathway databases
BRENDA	5.3.4.1. 248.
Gene expression databases
Genevestigator	Q63081.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR017936. Thioredoxin-like. IPR012336. Thioredoxin-like_fold. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	625007.

Entry information

Entry name

PDIA6_RAT

Accession

Primary (citable) accession number: Q63081
Secondary accession number(s): Q641Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 29, 2005
Last modified:	February 9, 2010
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)