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Protein

Phosphorylated adapter RNA export protein

Gene

Phax

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA (By similarity).By similarity

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • toxic substance binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-191859. snRNP Assembly.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylated adapter RNA export protein
Alternative name(s):
26 kDa resiniferatoxin-binding protein
RBP-26
RNA U small nuclear RNA export adapter protein
RTX-42
Resiniferatoxin-binding protein 2
Short name:
RBP-2
Gene namesi
Name:Phax
Synonyms:Rnuxa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi708448. Phax.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleoplasm By similarity
  • NucleusCajal body By similarity
  • Cytoplasm By similarity

  • Note: Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies.By similarity

GO - Cellular componenti

  • Cajal body Source: UniProtKB-SubCell
  • centrosome Source: Ensembl
  • cytoplasm Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 385384Phosphorylated adapter RNA export proteinPRO_0000239777Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei56 – 561PhosphoserineCombined sources
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources
Modified residuei217 – 2171PhosphoserineCombined sources
Modified residuei287 – 2871PhosphothreonineBy similarity
Modified residuei347 – 3471PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63068.
PRIDEiQ63068.

PTM databases

iPTMnetiQ63068.
PhosphoSiteiQ63068.

Expressioni

Tissue specificityi

Expressed in dorsal root ganglia and sensory neuron cell bodies.1 Publication

Gene expression databases

GenevisibleiQ63068. RN.

Interactioni

Subunit structurei

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80. Found in a complex with snoRNA. Interacts with NCBP2/CBP20 (By similarity).By similarity

Protein-protein interaction databases

BioGridi251908. 1 interaction.
STRINGi10116.ENSRNOP00000019627.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 320319Necessary for interaction with CBP80By similarityAdd
BLAST
Regioni219 – 319101Sufficient for poly U RNA-bindingBy similarityAdd
BLAST
Regioni270 – 2789Necessary for poly U RNA-binding and snRNA exportBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 744Nuclear localization signalBy similarity
Motifi120 – 12910Nuclear export signalBy similarity
Motifi189 – 1924Nuclear localization signalBy similarity

Sequence similaritiesi

Belongs to the PHAX family.Curated

Phylogenomic databases

eggNOGiKOG3948. Eukaryota.
ENOG4110D9C. LUCA.
GeneTreeiENSGT00390000011084.
HOGENOMiHOG000115525.
HOVERGENiHBG058976.
InParanoidiQ63068.
KOiK14291.
OMAiDAGDMED.
OrthoDBiEOG7Q5HF9.
PhylomeDBiQ63068.
TreeFamiTF321050.

Family and domain databases

InterProiIPR019385. PHAX_RNA-binding_domain.
[Graphical view]
PfamiPF10258. RNA_GG_bind. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEAGDMEE GQLSDSDSDM TVVPSDRPLQ MAKVLGGGGA ACAPVSNYRT
60 70 80 90 100
VKHVDSSEES LDSDDDCSLW KRKRQKCHSP PPKPEPFPFG QSGQKPALNG
110 120 130 140 150
GKKVNNIWGA VLQEQNQDAV ATELGILGME GTIDRSRQSE TYNYLLAKKL
160 170 180 190 200
AKKESQEYTK ELDKDLDEYM HGDKKPGSKE EENGQGHLKR KRPVRDRLGN
210 220 230 240 250
RVEMNYKGRY DITEEDSPEK VADEIAFRLQ EPKKDLIARV VTILGNKKAI
260 270 280 290 300
ELLMETAEVE QNGGLFIMNG SRRRTPGGVF LNLLKNTPSI SEEQIKDIFY
310 320 330 340 350
IENQKEYENK KAARKRRTQL LGKKMKEAIK SLNFQEDDDT SRETFASDTN
360 370 380
EALASLDEAQ EGPGETKLDA EDAIEVDHPQ DLDIF
Length:385
Mass (Da):43,166
Last modified:June 13, 2006 - v2
Checksum:iC53797EA193EAD5B
GO

Sequence cautioni

The sequence CAA48076.1 differs from that shown. Reason: Frameshift at position 230. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961P → Q in CAA48076 (PubMed:8015392).Curated
Sequence conflicti375 – 3751E → D (PubMed:8015392).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67877 mRNA. Translation: CAA48076.1. Frameshift.
BC086410 mRNA. Translation: AAH86410.1.
BC097932 mRNA. Translation: AAH97932.1.
PIRiI60184. S33448.
RefSeqiNP_775156.2. NM_173133.2.
UniGeneiRn.10317.

Genome annotation databases

EnsembliENSRNOT00000019627; ENSRNOP00000019627; ENSRNOG00000014459.
GeneIDi286917.
KEGGirno:286917.
UCSCiRGD:708448. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67877 mRNA. Translation: CAA48076.1. Frameshift.
BC086410 mRNA. Translation: AAH86410.1.
BC097932 mRNA. Translation: AAH97932.1.
PIRiI60184. S33448.
RefSeqiNP_775156.2. NM_173133.2.
UniGeneiRn.10317.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251908. 1 interaction.
STRINGi10116.ENSRNOP00000019627.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ63068.
PhosphoSiteiQ63068.

Proteomic databases

PaxDbiQ63068.
PRIDEiQ63068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019627; ENSRNOP00000019627; ENSRNOG00000014459.
GeneIDi286917.
KEGGirno:286917.
UCSCiRGD:708448. rat.

Organism-specific databases

CTDi51808.
RGDi708448. Phax.

Phylogenomic databases

eggNOGiKOG3948. Eukaryota.
ENOG4110D9C. LUCA.
GeneTreeiENSGT00390000011084.
HOGENOMiHOG000115525.
HOVERGENiHBG058976.
InParanoidiQ63068.
KOiK14291.
OMAiDAGDMED.
OrthoDBiEOG7Q5HF9.
PhylomeDBiQ63068.
TreeFamiTF321050.

Enzyme and pathway databases

ReactomeiR-RNO-191859. snRNP Assembly.
R-RNO-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

PROiQ63068.

Gene expression databases

GenevisibleiQ63068. RN.

Family and domain databases

InterProiIPR019385. PHAX_RNA-binding_domain.
[Graphical view]
PfamiPF10258. RNA_GG_bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a resiniferatoxin-binding protein."
    Ninkina N., Willoughby J., Beech M., Coore P., Wood J.
    Brain Res. Mol. Brain Res. 22:39-48(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Thymus.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-56; SER-57; SER-60; SER-63 AND SER-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPHAX_RAT
AccessioniPrimary (citable) accession number: Q63068
Secondary accession number(s): Q4QQW9, Q5RJZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: June 8, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.