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Protein

Phosphorylated adapter RNA export protein

Gene

Phax

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA (By similarity).By similarity

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • toxic substance binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-191859 snRNP Assembly
R-RNO-6807505 RNA polymerase II transcribes snRNA genes

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylated adapter RNA export protein
Alternative name(s):
26 kDa resiniferatoxin-binding protein
RBP-26
RNA U small nuclear RNA export adapter protein
RTX-42
Resiniferatoxin-binding protein 2
Short name:
RBP-2
Gene namesi
Name:Phax
Synonyms:Rnuxa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi708448 Phax

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002397772 – 385Phosphorylated adapter RNA export proteinAdd BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei14PhosphoserineCombined sources1
Modified residuei16PhosphoserineBy similarity1
Modified residuei56PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei287PhosphothreonineBy similarity1
Modified residuei347PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63068
PRIDEiQ63068

PTM databases

iPTMnetiQ63068
PhosphoSitePlusiQ63068

Expressioni

Tissue specificityi

Expressed in dorsal root ganglia and sensory neuron cell bodies.1 Publication

Gene expression databases

BgeeiENSRNOG00000014459
GenevisibleiQ63068 RN

Interactioni

Subunit structurei

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20 and m7G-capped RNA. Interacts with NCBP1/CBP80. Found in a complex with snoRNA. Interacts with NCBP2/CBP20 (By similarity).By similarity

Protein-protein interaction databases

BioGridi251908, 1 interactor
STRINGi10116.ENSRNOP00000019627

Structurei

3D structure databases

SMRiQ63068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 320Necessary for interaction with CBP80By similarityAdd BLAST319
Regioni219 – 319Sufficient for poly U RNA-bindingBy similarityAdd BLAST101
Regioni270 – 278Necessary for poly U RNA-binding and snRNA exportBy similarity9

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 74Nuclear localization signalBy similarity4
Motifi120 – 129Nuclear export signalBy similarity10
Motifi189 – 192Nuclear localization signalBy similarity4

Sequence similaritiesi

Belongs to the PHAX family.Curated

Phylogenomic databases

eggNOGiKOG3948 Eukaryota
ENOG4110D9C LUCA
GeneTreeiENSGT00390000011084
HOGENOMiHOG000115525
HOVERGENiHBG058976
InParanoidiQ63068
KOiK14291
OMAiSQEHTKE
OrthoDBiEOG091G0XKP
PhylomeDBiQ63068
TreeFamiTF321050

Family and domain databases

Gene3Di1.10.10.1440, 1 hit
InterProiView protein in InterPro
IPR019385 PHAX_RNA-binding_domain
IPR038092 PHAX_RNA-binding_sf
PfamiView protein in Pfam
PF10258 RNA_GG_bind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEAGDMEE GQLSDSDSDM TVVPSDRPLQ MAKVLGGGGA ACAPVSNYRT
60 70 80 90 100
VKHVDSSEES LDSDDDCSLW KRKRQKCHSP PPKPEPFPFG QSGQKPALNG
110 120 130 140 150
GKKVNNIWGA VLQEQNQDAV ATELGILGME GTIDRSRQSE TYNYLLAKKL
160 170 180 190 200
AKKESQEYTK ELDKDLDEYM HGDKKPGSKE EENGQGHLKR KRPVRDRLGN
210 220 230 240 250
RVEMNYKGRY DITEEDSPEK VADEIAFRLQ EPKKDLIARV VTILGNKKAI
260 270 280 290 300
ELLMETAEVE QNGGLFIMNG SRRRTPGGVF LNLLKNTPSI SEEQIKDIFY
310 320 330 340 350
IENQKEYENK KAARKRRTQL LGKKMKEAIK SLNFQEDDDT SRETFASDTN
360 370 380
EALASLDEAQ EGPGETKLDA EDAIEVDHPQ DLDIF
Length:385
Mass (Da):43,166
Last modified:June 13, 2006 - v2
Checksum:iC53797EA193EAD5B
GO

Sequence cautioni

The sequence CAA48076 differs from that shown. Reason: Frameshift at position 230.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96P → Q in CAA48076 (PubMed:8015392).Curated1
Sequence conflicti375E → D (PubMed:8015392).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67877 mRNA Translation: CAA48076.1 Frameshift.
BC086410 mRNA Translation: AAH86410.1
BC097932 mRNA Translation: AAH97932.1
PIRiI60184 S33448
RefSeqiNP_775156.2, NM_173133.2
UniGeneiRn.10317

Genome annotation databases

EnsembliENSRNOT00000019627; ENSRNOP00000019627; ENSRNOG00000014459
GeneIDi286917
KEGGirno:286917
UCSCiRGD:708448 rat

Similar proteinsi

Entry informationi

Entry nameiPHAX_RAT
AccessioniPrimary (citable) accession number: Q63068
Secondary accession number(s): Q4QQW9, Q5RJZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: March 28, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health