Reviewed, UniProtKB/Swiss-Prot Q63065 (PDK1_RAT)

Names and origin

Protein names

Recommended name:
    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
    EC=2.7.11.2
Alternative name(s):
    Pyruvate dehydrogenase kinase isoform 1
    PDK p48

Gene names

Name:	Pdk1
Synonyms:	Pdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Expressed predominantly in the heart.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Inferred from mutant phenotype. Source: RGD glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell pyruvate dehydrogenase complex Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from direct assay. Source: RGD protein complex binding Inferred from direct assay. Source: RGD protein heterodimerization activity Inferred from direct assay. Source: RGD protein homodimerization activity Inferred from direct assay. Source: RGD pyruvate dehydrogenase (acetyl-transferring) kinase activity Ref.1 Inferred from direct assay. Source: RGD two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 434	408	[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial		PRO_0000023439
Regions
	Domain	161 – 391	231	Histidine kinase

Sequences

	Sequence		Length	Mass (Da)
	Q63065-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A8C304507D64ED61	FASTA	434	49,081
	10 20 30 40 50 60 MRLARLLRGG TSVRPLCAVP CASRSLASDS ASGSGPASES GVPGQVDFYA RFSPSPLSMK 70 80 90 100 110 120 QFLDFGSVNA CEKTSFMFLR QELPVRLANI MKEISLLPDN LLRTPSVQLV QSWYIQSLQE 130 140 150 160 170 180 LLDFKDKSAE DAKTIYEFTD TVIRIRNRHN DVIPTMAQGV TEYKESFGVD PVTSQNVQYF 190 200 210 220 230 240 LDRFYMSRIS IRMLLNQHSL LFGGKGSPSH RKHIGSINPN CDVVEVIKDG YENARRLCDL 250 260 270 280 290 300 YYVNSPELEL EELNAKSPGQ PIQVVYVPSH LYHMVFELFK NAMRATMEHH ADKGVYPPIQ 310 320 330 340 350 360 VHVTLGEEDL TVKMSDRGGG VPLRKIDRLF NYMYSTAPRP RVETSRAVPL AGFGYGLPIS 370 380 390 400 410 420 RLYAQYFQGD LKLYSLEGYG TDAVIYIKAL STESIERLPV YNKAAWKHYR TNHEADDWCV 430 PSREPKDMTT FRSS

References

[1]	"Primary structure of pyruvate dehydrogenase kinase establishes a new family of eukaryotic protein kinases." Popov K.M., Kedishvili N.Y., Zhao Y., Shimomura Y., Crabb D.W., Harris R.A. J. Biol. Chem. 268:26602-26606(1993) [PubMed: 8253790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L22294 mRNA. Translation: AAA62851.1.
IPI	IPI00204957.
PIR	A49686.
UniGene	Rn.11185
3D structure databases
HSSP	HSSP built from PDB template 1JM6 based on UniProtKB Q64536.
SMR	Q63065. Positions 42-429.
ModBase	Search...
Protein-protein interaction databases
STRING	Q63065.
Genome annotation databases
Ensembl	ENSRNOT00000002072; ENSRNOP00000002072; ENSRNOG00000001517; Rattus norvegicus. [Genome view]
UCSC	NM_053826. rat.
Organism-specific databases
RGD	69427. Pdk1.
Phylogenomic databases
HOVERGEN	Q63065.
Enzyme and pathway databases
BRENDA	2.7.11.2. 248.
Gene expression databases
ArrayExpress	Q63065.
Genevestigator	Q63065.
GermOnline	ENSRNOG00000001517. Rattus norvegicus.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR018955. BCDHK/PDK_mit. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDK1_RAT

Accession

Primary (citable) accession number: Q63065

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)