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Q63053 (ARC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Activity-regulated cytoskeleton-associated protein
Alternative name(s):
ARC/ARG3.1
Activity-regulated gene 3.1 protein
Short name=Arg3.1
Gene names
Name:Arc
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity. Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors. Ref.4 Ref.5

Subunit structure

Interacts with SH3GL1/endophilin-2, SH3GL3/endophilin-3 and DNM2/DYN2. Ref.3

Subcellular location

Cytoplasmcytoskeleton. Endosome. Cytoplasmic vesiclesecretory vesicleacrosome By similarity. Cytoplasmcell cortex. Cell projectiondendrite. Cell projectiondendritic spine. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density. Cell junctionsynapse. Note: Enriched on the plasma membrane By similarity. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail. Ref.1 Ref.2 Ref.3

Tissue specificity

Expressed exclusively in certain parts of the brain including cortex and molecular layer of the hippocampus. Typically expressed at high level in a minority of neurons. Basal expression higher in cortex than in hippocampus, highest in visual cortex. Ref.1 Ref.2

Developmental stage

Expressed during postnatal development from day 8. Highest expression around day 23 with moderate levels expressed to adulthood. Ref.1

Induction

By synaptic activity in hippocampus, cerebral cortex, amygdala and hypothalamus. Highest level in hippocampus within 30 min, dropping to basal levels within 24 hours. By NGF and EGF in fibroblasts. Ref.1 Ref.2

Miscellaneous

Widely used as activity-dependent neuronal marker to identify recently activated neurons in behavioral studies By similarity. UniProtKB Q9WV31

Neurons overexpressing Arc show reduced AMPA receptor-dependent synaptic transmission associated with reduced surface levels of AMPA receptor. This effect is dependent on NMDA receptor activity. Ref.4 Ref.5

Transcripts enriched in dendrites may be translated locally. Ref.2

Sequence similarities

Belongs to the ARC/ARG3.1 family.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Endosome
Membrane
Postsynaptic cell membrane
Synapse
   DomainCoiled coil
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from electronic annotation. Source: Ensembl

cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

endocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

endoderm development

Inferred from electronic annotation. Source: Ensembl

learning

Inferred from expression pattern PubMed 20111591. Source: RGD

regulation of cell morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of neuronal synaptic plasticity

Inferred from expression pattern Ref.1. Source: RGD

   Cellular_componentacrosomal vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

actin cytoskeleton

Inferred from direct assay Ref.1. Source: RGD

cell cortex

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Traceable author statement PubMed 12451105. Source: RGD

dendritic spine

Inferred from electronic annotation. Source: UniProtKB-SubCell

endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

postsynaptic density

Inferred from electronic annotation. Source: UniProtKB-SubCell

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Camk2bP084136EBI-5275794,EBI-916155
PSEN1P497683EBI-5275794,EBI-2606326From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Activity-regulated cytoskeleton-associated protein
PRO_0000273287

Regions

Region89 – 10012Binds SH3GL1 or SH3GL3 Ref.3
Region195 – 21420Required for binding DNM2 Ref.3
Coiled coil54 – 7825 Potential

Experimental info

Sequence conflict2091V → L in AAA68695. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q63053 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4E95B46B75853CA5

FASTA39645,353
        10         20         30         40         50         60 
MELDHMTTGG LHAYPAPRGG PAAKPNVILQ IGKCRAEMLE HVRRTHRHLL TEVSKQVERE 

        70         80         90        100        110        120 
LKGLHRSVGK LENNLDGYVP TGDSQRWKKS IKACLCRCQE TIANLERWVK REMHVWREVF 

       130        140        150        160        170        180 
YRLERWADRL ESMGGKYPVG SEPARHTVSV GVGGPEPYCQ EADGYDYTVS PYAITPPPAA 

       190        200        210        220        230        240 
GELPEQESVG AQQYQSWVPG EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS 

       250        260        270        280        290        300 
QIQNHMNGPA KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELDL PQKQGEPLDQ 

       310        320        330        340        350        360 
FLWRKRDLYQ TLYVDAEEEE IIQYVVGTLQ PKFKRFLRHP LPKTLEQLIQ RGMEVQDGLE 

       370        380        390 
QAAEPSVTPL PTEDETEALT PALTSESVAS DRTQPE 

« Hide

References

[1]"Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites."
Lyford G.L., Yamagata K., Kaufmann W.E., Barnes C.A., Sanders L.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Lanahan A.A., Worley P.F.
Neuron 14:433-445(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Tissue: Hippocampus.
[2]"Somatodendritic expression of an immediate early gene is regulated by synaptic activity."
Link W., Konietzko U., Kauselmann G., Krug M., Schwanke B., Frey U., Kuhl D.
Proc. Natl. Acad. Sci. U.S.A. 92:5734-5738(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[3]"Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking."
Chowdhury S., Shepherd J.D., Okuno H., Lyford G., Petralia R.S., Plath N., Kuhl D., Huganir R.L., Worley P.F.
Neuron 52:445-459(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DNM2; SH3GL1 AND SH3GL3.
[4]"Increased expression of the immediate-early gene arc/arg3.1 reduces AMPA receptor-mediated synaptic transmission."
Rial Verde E.M., Lee-Osbourne J., Worley P.F., Malinow R., Cline H.T.
Neuron 52:461-474(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Arc/Arg3.1 mediates homeostatic synaptic scaling of AMPA Receptors."
Shepherd J.D., Rumbaugh G., Wu J., Chowdhury S., Plath N., Kuhl D., Huganir R.L., Worley P.F.
Neuron 52:475-484(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19866 mRNA. Translation: AAA68695.1.
Z46925 mRNA. Translation: CAA87033.1.
PIRI58168.
RefSeqNP_062234.1. NM_019361.1.
XP_006241796.1. XM_006241734.1.
UniGeneRn.10086.

3D structure databases

ProteinModelPortalQ63053.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248539. 2 interactions.
IntActQ63053. 6 interactions.

PTM databases

PhosphoSiteQ63053.

Proteomic databases

PaxDbQ63053.
PRIDEQ63053.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000067442; ENSRNOP00000063719; ENSRNOG00000043465.
GeneID54323.
KEGGrno:54323.
UCSCRGD:62037. rat.

Organism-specific databases

CTD23237.
RGD62037. Arc.

Phylogenomic databases

eggNOGNOG81608.
GeneTreeENSGT00390000003914.
HOGENOMHOG000034037.
HOVERGENHBG080862.
KOK15867.
OMARCQETIA.
OrthoDBEOG75B855.
PhylomeDBQ63053.
TreeFamTF335604.

Gene expression databases

GenevestigatorQ63053.

Family and domain databases

InterProIPR023263. Activity-reg_cytoskelet-assoc.
[Graphical view]
PANTHERPTHR15962. PTHR15962. 1 hit.
PRINTSPR02027. ARCARG31.
ProtoNetSearch...

Other

NextBio611018.
PROQ63053.

Entry information

Entry nameARC_RAT
AccessionPrimary (citable) accession number: Q63053
Secondary accession number(s): Q62743
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families