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Protein

Activity-regulated cytoskeleton-associated protein

Gene

Arc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration (By similarity). Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors.By similarity2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Activity-regulated cytoskeleton-associated protein
Alternative name(s):
ARC/ARG3.1
Activity-regulated gene 3.1 protein
Short name:
Arg3.1
Gene namesi
Name:ArcImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi62037. Arc.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB-SubCell
  • actin cytoskeleton Source: RGD
  • cell cortex Source: UniProtKB-SubCell
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: RGD
  • dendritic spine Source: UniProtKB-SubCell
  • endosome Source: UniProtKB-SubCell
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Activity-regulated cytoskeleton-associated proteinPRO_0000273287Add
BLAST

Proteomic databases

PaxDbiQ63053.
PRIDEiQ63053.

PTM databases

PhosphoSiteiQ63053.

Expressioni

Tissue specificityi

Expressed exclusively in certain parts of the brain including cortex and molecular layer of the hippocampus. Typically expressed at high level in a minority of neurons. Basal expression higher in cortex than in hippocampus, highest in visual cortex.2 Publications

Developmental stagei

Expressed during postnatal development from day 8. Highest expression around day 23 with moderate levels expressed to adulthood.1 Publication

Inductioni

By synaptic activity in hippocampus, cerebral cortex, amygdala and hypothalamus. Highest level in hippocampus within 30 min, dropping to basal levels within 24 hours. By NGF and EGF in fibroblasts.2 Publications

Gene expression databases

GenevisibleiQ63053. RN.

Interactioni

Subunit structurei

Interacts with SH3GL1/endophilin-2, SH3GL3/endophilin-3 and DNM2/DYN2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Camk2bP084136EBI-5275794,EBI-916155
PSEN1P497683EBI-5275794,EBI-2606326From a different organism.

Protein-protein interaction databases

BioGridi248539. 2 interactions.
IntActiQ63053. 6 interactions.
STRINGi10116.ENSRNOP00000063719.

Structurei

3D structure databases

ProteinModelPortaliQ63053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 10012Binds SH3GL1 or SH3GL31 PublicationAdd
BLAST
Regioni195 – 21420Required for binding DNM21 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili54 – 7825Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the ARC/ARG3.1 family.Sequence Analysis

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG81608.
GeneTreeiENSGT00390000003914.
HOGENOMiHOG000034037.
HOVERGENiHBG080862.
InParanoidiQ63053.
KOiK15867.
OMAiRCQETIA.
OrthoDBiEOG75B855.
PhylomeDBiQ63053.
TreeFamiTF335604.

Family and domain databases

InterProiIPR023263. Activity-reg_cytoskelet-assoc.
[Graphical view]
PANTHERiPTHR15962. PTHR15962. 1 hit.
PRINTSiPR02027. ARCARG31.

Sequencei

Sequence statusi: Complete.

Q63053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDHMTTGG LHAYPAPRGG PAAKPNVILQ IGKCRAEMLE HVRRTHRHLL
60 70 80 90 100
TEVSKQVERE LKGLHRSVGK LENNLDGYVP TGDSQRWKKS IKACLCRCQE
110 120 130 140 150
TIANLERWVK REMHVWREVF YRLERWADRL ESMGGKYPVG SEPARHTVSV
160 170 180 190 200
GVGGPEPYCQ EADGYDYTVS PYAITPPPAA GELPEQESVG AQQYQSWVPG
210 220 230 240 250
EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS QIQNHMNGPA
260 270 280 290 300
KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELDL PQKQGEPLDQ
310 320 330 340 350
FLWRKRDLYQ TLYVDAEEEE IIQYVVGTLQ PKFKRFLRHP LPKTLEQLIQ
360 370 380 390
RGMEVQDGLE QAAEPSVTPL PTEDETEALT PALTSESVAS DRTQPE
Length:396
Mass (Da):45,353
Last modified:November 1, 1996 - v1
Checksum:i4E95B46B75853CA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091V → L in AAA68695 (PubMed:7857651).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19866 mRNA. Translation: AAA68695.1.
Z46925 mRNA. Translation: CAA87033.1.
PIRiI58168.
RefSeqiNP_062234.1. NM_019361.1.
XP_008763812.1. XM_008765590.1.
XP_008763813.1. XM_008765591.1.
UniGeneiRn.10086.

Genome annotation databases

EnsembliENSRNOT00000067442; ENSRNOP00000063719; ENSRNOG00000043465.
ENSRNOT00000076998; ENSRNOP00000068090; ENSRNOG00000043465.
GeneIDi54323.
KEGGirno:54323.
UCSCiRGD:62037. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19866 mRNA. Translation: AAA68695.1.
Z46925 mRNA. Translation: CAA87033.1.
PIRiI58168.
RefSeqiNP_062234.1. NM_019361.1.
XP_008763812.1. XM_008765590.1.
XP_008763813.1. XM_008765591.1.
UniGeneiRn.10086.

3D structure databases

ProteinModelPortaliQ63053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248539. 2 interactions.
IntActiQ63053. 6 interactions.
STRINGi10116.ENSRNOP00000063719.

PTM databases

PhosphoSiteiQ63053.

Proteomic databases

PaxDbiQ63053.
PRIDEiQ63053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067442; ENSRNOP00000063719; ENSRNOG00000043465.
ENSRNOT00000076998; ENSRNOP00000068090; ENSRNOG00000043465.
GeneIDi54323.
KEGGirno:54323.
UCSCiRGD:62037. rat.

Organism-specific databases

CTDi23237.
RGDi62037. Arc.

Phylogenomic databases

eggNOGiNOG81608.
GeneTreeiENSGT00390000003914.
HOGENOMiHOG000034037.
HOVERGENiHBG080862.
InParanoidiQ63053.
KOiK15867.
OMAiRCQETIA.
OrthoDBiEOG75B855.
PhylomeDBiQ63053.
TreeFamiTF335604.

Miscellaneous databases

NextBioi611018.
PROiQ63053.

Gene expression databases

GenevisibleiQ63053. RN.

Family and domain databases

InterProiIPR023263. Activity-reg_cytoskelet-assoc.
[Graphical view]
PANTHERiPTHR15962. PTHR15962. 1 hit.
PRINTSiPR02027. ARCARG31.
ProtoNetiSearch...

Publicationsi

  1. "Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites."
    Lyford G.L., Yamagata K., Kaufmann W.E., Barnes C.A., Sanders L.K., Copeland N.G., Gilbert D.J., Jenkins N.A., Lanahan A.A., Worley P.F.
    Neuron 14:433-445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Hippocampus1 Publication.
  2. "Somatodendritic expression of an immediate early gene is regulated by synaptic activity."
    Link W., Konietzko U., Kauselmann G., Krug M., Schwanke B., Frey U., Kuhl D.
    Proc. Natl. Acad. Sci. U.S.A. 92:5734-5738(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-DawleyImported.
    Tissue: HippocampusImported.
  3. "Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking."
    Chowdhury S., Shepherd J.D., Okuno H., Lyford G., Petralia R.S., Plath N., Kuhl D., Huganir R.L., Worley P.F.
    Neuron 52:445-459(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DNM2; SH3GL1 AND SH3GL3.
  4. "Increased expression of the immediate-early gene arc/arg3.1 reduces AMPA receptor-mediated synaptic transmission."
    Rial Verde E.M., Lee-Osbourne J., Worley P.F., Malinow R., Cline H.T.
    Neuron 52:461-474(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Arc/Arg3.1 mediates homeostatic synaptic scaling of AMPA Receptors."
    Shepherd J.D., Rumbaugh G., Wu J., Chowdhury S., Plath N., Kuhl D., Huganir R.L., Worley P.F.
    Neuron 52:475-484(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiARC_RAT
AccessioniPrimary (citable) accession number: Q63053
Secondary accession number(s): Q62743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Widely used as activity-dependent neuronal marker to identify recently activated neurons in behavioral studies.By similarity
Neurons overexpressing Arc show reduced AMPA receptor-dependent synaptic transmission associated with reduced surface levels of AMPA receptor. This effect is dependent on NMDA receptor activity.2 Publications
Transcripts enriched in dendrites may be translated locally.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.