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Protein

Runt-related transcription factor 1

Gene

Runx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121Chloride 1PROSITE-ProRule annotation
Binding sitei116 – 1161Chloride 1; via amide nitrogenPROSITE-ProRule annotation
Binding sitei139 – 1391Chloride 2PROSITE-ProRule annotation
Binding sitei170 – 1701Chloride 2; via amide nitrogenPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of granulocyte differentiation Source: UniProtKB
  • positive regulation of progesterone secretion Source: RGD
  • regulation of transcription, DNA-templated Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-549127. Organic cation transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 1
Alternative name(s):
Acute myeloid leukemia 1 protein
Core-binding factor subunit alpha-2
Short name:
CBF-alpha-2
Oncogene AML-1
Polyomavirus enhancer-binding protein 2 alpha B subunit
Short name:
PEA2-alpha B
Short name:
PEBP2-alpha B
Gene namesi
Name:Runx1
Synonyms:Aml1, Cbfa2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2283. Runx1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Runt-related transcription factor 1PRO_0000174657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphothreonineCombined sources
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei24 – 241N6-acetyllysineBy similarity
Modified residuei43 – 431N6-acetyllysineBy similarity
Modified residuei212 – 2121PhosphoserineBy similarity
Modified residuei249 – 2491Phosphoserine; by HIPK2By similarity
Modified residuei266 – 2661PhosphoserineBy similarity
Modified residuei267 – 2671PhosphoserineBy similarity
Modified residuei272 – 2721Phosphothreonine; by HIPK2By similarity
Modified residuei275 – 2751Phosphoserine; by HIPK2By similarity
Modified residuei433 – 4331PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A (By similarity).By similarity
Methylated.By similarity
Phosphorylated in Ser-249 Thr-272 and Ser-275 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ63046.
PRIDEiQ63046.

PTM databases

iPTMnetiQ63046.
PhosphoSiteiQ63046.

Expressioni

Tissue specificityi

Expressed in skeletal muscle.1 Publication

Inductioni

Expression increases following denervation.1 Publication

Gene expression databases

ExpressionAtlasiQ63046. baseline and differential.
GenevisibleiQ63046. RN.

Interactioni

Subunit structurei

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1 and HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Found in a complex with PRMT5, RUNX1 and CBFB. Interacts with FOXP3.By similarity

Protein-protein interaction databases

BioGridi248410. 1 interaction.
STRINGi10116.ENSRNOP00000002313.

Structurei

3D structure databases

ProteinModelPortaliQ63046.
SMRiQ63046. Positions 54-178, 318-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 178129RuntPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 845Interaction with DNABy similarity
Regioni135 – 1439Interaction with DNABy similarity
Regioni168 – 17710Interaction with DNABy similarity
Regioni290 – 36980Interaction with KAT6ABy similarityAdd
BLAST
Regioni306 – 39893Interaction with KAT6BBy similarityAdd
BLAST
Regioni360 – 40041Interaction with FOXP3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi187 – 450264Pro/Ser/Thr-richAdd
BLAST

Domaini

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Sequence similaritiesi

Contains 1 Runt domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3982. Eukaryota.
ENOG4111J4Y. LUCA.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.
InParanoidiQ63046.
KOiK08367.
OMAiFSMMAGG.
OrthoDBiEOG7WQ7TV.
PhylomeDBiQ63046.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51062. RUNT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIPVDASTS RRFTPPSTAL SPGKMSEALP LGAPDGGAAL ASKLRSGDRS
60 70 80 90 100
MVEVLADHPG ELVRTDSPNF LCSVLPTHWR CNKTLPIAFK VVALGDVPDG
110 120 130 140 150
TLVTVMAGND ENYSAELRNA TAAMKNQVAR FNDLRFVGRS GRGKSFTLTI
160 170 180 190 200
TVFTNPPQVA TYHRAIKITV DGPREPRRHR QKLDDQTKPG SLSFSERLSE
210 220 230 240 250
LEQLRRTAMR VSPHHPAPTP NPRASLNHST AFNPQPQSQM QDARQIQPSP
260 270 280 290 300
PWSYDQSYQY LGSITSSVHP ATPISPGRAS GMTSLSAELS SRLSTAPDLT
310 320 330 340 350
AFGDPRQFPT LPSISDPRMH YPGAFTYSPP VTSGIGIGMS AMSSTSRYHT
360 370 380 390 400
YLPPPYPGSS QAQAGPFQTG SPSYHLYYGT SAGSYQFSMV GGERSPPRIL
410 420 430 440 450
PPCTNASTGA ALLNPSLPSQ SDVVETEGSH SNSPTNMPPA RLEEAVWRPY
Length:450
Mass (Da):48,556
Last modified:November 1, 1996 - v1
Checksum:i4B53AD706D487AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35271 mRNA. Translation: AAA66191.1.
RefSeqiNP_059021.1. NM_017325.2.
UniGeneiRn.11201.

Genome annotation databases

EnsembliENSRNOT00000002313; ENSRNOP00000002313; ENSRNOG00000001704.
GeneIDi50662.
KEGGirno:50662.
UCSCiRGD:2283. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35271 mRNA. Translation: AAA66191.1.
RefSeqiNP_059021.1. NM_017325.2.
UniGeneiRn.11201.

3D structure databases

ProteinModelPortaliQ63046.
SMRiQ63046. Positions 54-178, 318-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248410. 1 interaction.
STRINGi10116.ENSRNOP00000002313.

PTM databases

iPTMnetiQ63046.
PhosphoSiteiQ63046.

Proteomic databases

PaxDbiQ63046.
PRIDEiQ63046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002313; ENSRNOP00000002313; ENSRNOG00000001704.
GeneIDi50662.
KEGGirno:50662.
UCSCiRGD:2283. rat.

Organism-specific databases

CTDi861.
RGDi2283. Runx1.

Phylogenomic databases

eggNOGiKOG3982. Eukaryota.
ENOG4111J4Y. LUCA.
GeneTreeiENSGT00390000016964.
HOGENOMiHOG000045616.
HOVERGENiHBG060268.
InParanoidiQ63046.
KOiK08367.
OMAiFSMMAGG.
OrthoDBiEOG7WQ7TV.
PhylomeDBiQ63046.

Enzyme and pathway databases

ReactomeiR-RNO-549127. Organic cation transport.

Miscellaneous databases

PROiQ63046.

Gene expression databases

ExpressionAtlasiQ63046. baseline and differential.
GenevisibleiQ63046. RN.

Family and domain databases

Gene3Di2.60.40.720. 1 hit.
4.10.770.10. 1 hit.
InterProiIPR000040. AML1_Runt.
IPR008967. p53-like_TF_DNA-bd.
IPR012346. p53/RUNT-type_TF_DNA-bd.
IPR013524. Runt_dom.
IPR027384. Runx_central_dom.
IPR013711. RunxI_C_dom.
IPR016554. TF_Runt-rel_RUNX.
[Graphical view]
PANTHERiPTHR11950. PTHR11950. 1 hit.
PfamiPF00853. Runt. 1 hit.
PF08504. RunxI. 1 hit.
[Graphical view]
PIRSFiPIRSF009374. TF_Runt-rel_RUNX. 1 hit.
PRINTSiPR00967. ONCOGENEAML1.
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS51062. RUNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AML1 is expressed in skeletal muscle and is regulated by innervation."
    Zhu X., Yeadon J.E., Burden S.J.
    Mol. Cell. Biol. 14:8051-8057(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Skeletal muscle.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14 AND SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRUNX1_RAT
AccessioniPrimary (citable) accession number: Q63046
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.