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Protein

Runt-related transcription factor 1

Gene

Runx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (By similarity). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112Chloride 1PROSITE-ProRule annotation1
Binding sitei116Chloride 1; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei139Chloride 2PROSITE-ProRule annotation1
Binding sitei170Chloride 2; via amide nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-549127 Organic cation transport
R-RNO-8877330 RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs)
R-RNO-8931987 RUNX1 regulates estrogen receptor mediated transcription
R-RNO-8934593 Regulation of RUNX1 Expression and Activity
R-RNO-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-RNO-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-RNO-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-RNO-8939245 RUNX1 regulates transcription of genes involved in BCR signaling
R-RNO-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-RNO-8939247 RUNX1 regulates transcription of genes involved in interleukin signaling
R-RNO-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Runt-related transcription factor 1
Alternative name(s):
Acute myeloid leukemia 1 protein
Core-binding factor subunit alpha-2
Short name:
CBF-alpha-2
Oncogene AML-1
Polyomavirus enhancer-binding protein 2 alpha B subunit
Short name:
PEA2-alpha B
Short name:
PEBP2-alpha B
Gene namesi
Name:Runx1
Synonyms:Aml1, Cbfa2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2283 Runx1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746571 – 450Runt-related transcription factor 1Add BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphothreonineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei24N6-acetyllysineBy similarity1
Modified residuei43N6-acetyllysineBy similarity1
Modified residuei193PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei249Phosphoserine; by HIPK2By similarity1
Modified residuei266PhosphoserineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei272Phosphothreonine; by HIPK2By similarity1
Modified residuei275Phosphoserine; by HIPK2By similarity1
Modified residuei295PhosphothreonineBy similarity1
Modified residuei433PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A (By similarity).By similarity
Methylated.By similarity
Phosphorylated in Ser-249 Thr-272 and Ser-275 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ63046
PRIDEiQ63046

PTM databases

iPTMnetiQ63046
PhosphoSitePlusiQ63046

Expressioni

Tissue specificityi

Expressed in skeletal muscle.1 Publication

Inductioni

Expression increases following denervation.1 Publication

Gene expression databases

BgeeiENSRNOG00000001704
ExpressionAtlasiQ63046 baseline and differential
GenevisibleiQ63046 RN

Interactioni

Subunit structurei

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1 and HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Found in a complex with PRMT5, RUNX1 and CBFB. Interacts with FOXP3. Interacts with TBX21.By similarity

Protein-protein interaction databases

BioGridi248410, 1 interactor
ELMiQ63046
STRINGi10116.ENSRNOP00000002313

Structurei

3D structure databases

ProteinModelPortaliQ63046
SMRiQ63046
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 178RuntPROSITE-ProRule annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 84Interaction with DNABy similarity5
Regioni135 – 143Interaction with DNABy similarity9
Regioni168 – 177Interaction with DNABy similarity10
Regioni290 – 369Interaction with KAT6ABy similarityAdd BLAST80
Regioni306 – 398Interaction with KAT6BBy similarityAdd BLAST93
Regioni360 – 400Interaction with FOXP3By similarityAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi187 – 450Pro/Ser/Thr-richAdd BLAST264

Domaini

A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.

Phylogenomic databases

eggNOGiKOG3982 Eukaryota
ENOG4111J4Y LUCA
GeneTreeiENSGT00390000016964
HOGENOMiHOG000045616
HOVERGENiHBG060268
InParanoidiQ63046
KOiK08367
PhylomeDBiQ63046

Family and domain databases

Gene3Di2.60.40.720, 1 hit
4.10.770.10, 1 hit
InterProiView protein in InterPro
IPR000040 AML1_Runt
IPR008967 p53-like_TF_DNA-bd
IPR012346 p53/RUNT-type_TF_DNA-bd_sf
IPR013524 Runt_dom
IPR027384 Runx_central_dom_sf
IPR013711 RunxI_C_dom
IPR016554 TF_Runt-rel_RUNX
PANTHERiPTHR11950 PTHR11950, 1 hit
PfamiView protein in Pfam
PF00853 Runt, 1 hit
PF08504 RunxI, 1 hit
PIRSFiPIRSF009374 TF_Runt-rel_RUNX, 1 hit
PRINTSiPR00967 ONCOGENEAML1
SUPFAMiSSF49417 SSF49417, 1 hit
PROSITEiView protein in PROSITE
PS51062 RUNT, 1 hit

Sequencei

Sequence statusi: Complete.

Q63046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIPVDASTS RRFTPPSTAL SPGKMSEALP LGAPDGGAAL ASKLRSGDRS
60 70 80 90 100
MVEVLADHPG ELVRTDSPNF LCSVLPTHWR CNKTLPIAFK VVALGDVPDG
110 120 130 140 150
TLVTVMAGND ENYSAELRNA TAAMKNQVAR FNDLRFVGRS GRGKSFTLTI
160 170 180 190 200
TVFTNPPQVA TYHRAIKITV DGPREPRRHR QKLDDQTKPG SLSFSERLSE
210 220 230 240 250
LEQLRRTAMR VSPHHPAPTP NPRASLNHST AFNPQPQSQM QDARQIQPSP
260 270 280 290 300
PWSYDQSYQY LGSITSSVHP ATPISPGRAS GMTSLSAELS SRLSTAPDLT
310 320 330 340 350
AFGDPRQFPT LPSISDPRMH YPGAFTYSPP VTSGIGIGMS AMSSTSRYHT
360 370 380 390 400
YLPPPYPGSS QAQAGPFQTG SPSYHLYYGT SAGSYQFSMV GGERSPPRIL
410 420 430 440 450
PPCTNASTGA ALLNPSLPSQ SDVVETEGSH SNSPTNMPPA RLEEAVWRPY
Length:450
Mass (Da):48,556
Last modified:November 1, 1996 - v1
Checksum:i4B53AD706D487AC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35271 mRNA Translation: AAA66191.1
RefSeqiNP_059021.1, NM_017325.2
UniGeneiRn.11201

Genome annotation databases

EnsembliENSRNOT00000002313; ENSRNOP00000002313; ENSRNOG00000001704
GeneIDi50662
KEGGirno:50662
UCSCiRGD:2283 rat

Similar proteinsi

Entry informationi

Entry nameiRUNX1_RAT
AccessioniPrimary (citable) accession number: Q63046
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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