Reviewed, UniProtKB/Swiss-Prot Q63042 (ALR_RAT)

Names and origin

Protein names

Recommended name:
    FAD-linked sulfhydryl oxidase ALR
    EC=1.8.3.2
Alternative name(s):
    Augmenter of liver regeneration

Gene names

Name:	Gfer
Synonyms:	Alr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. May have a function in liver regeneration and spermatogenesis. Ref.1
Catalytic activity	4 R'C(R)SH + O2 = 2 R'C(R)S-S(R)CR' + 2 H2O.
Cofactor	FAD.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

FAD-linked sulfhydryl oxidase ALR

PRO_0000208550

Regions

Domain

88 – 188

101

ERV/ALR sulfhydryl oxidase

Region

171 – 188

18

FAD-binding

Amino acid modifications

Disulfide bond

88

Interchain (with C-197)

Disulfide bond

135 ↔ 138

Redox-active Potential

Disulfide bond

164 ↔ 181

Disulfide bond

197

Interchain (with C-88)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

198

Helix Strand Turn

Details...

Helix

92 – 108

17

Helix

116 – 132

17

Helix

136 – 148

13

Helix

156 – 173

18

Helix

181 – 183

3

Helix

184 – 188

5

Sequences

	Sequence		Length	Mass (Da)
	Q63042-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 46AD9BEC2EF0C393	FASTA	198	22,837
	10 20 30 40 50 60 MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA APAPKGLEHG 70 80 90 100 110 120 KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW AFLHTLAAYY PDMPTPEQQQ 130 140 150 160 170 180 DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD 190 CSRVDERWRD GWKDGSCD

References

[1]	"Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution." Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E. Proc. Natl. Acad. Sci. U.S.A. 91:8142-8146(1994) [PubMed: 8058770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN LIVER REGENERATION. Strain: Fischer 344. Tissue: Liver.
[2]	Erratum Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E. Proc. Natl. Acad. Sci. U.S.A. 92:3076-3076(1995) [PubMed: 7708779] [Abstract]
[3]	"The DNA sequence of augmenter of liver regeneration from rat's genome." Dong J., Cheng J., Wang Q.H., Liu Y.Z., Wang G., Shi S.S., Zhong Y.W., Xia X.B., Song H.B., Yang J.Z., Liu Y. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
[4]	"The crystal structure of augmenter of liver regeneration: a mammalian FAD-dependent sulfhydryl oxidase." Wu C.-K., Dailey T.A., Dailey H.A., Wang B.-C., Rose J.P. Protein Sci. 12:1109-1118(2003) [PubMed: 12717032] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D30735 mRNA. Translation: BAA06399.1. Different initiation.
AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.

IPI

IPI00326141.

PIR

I80184.

RefSeq

NP_037354.1.

UniGene

Rn.11039

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OQC	X-ray	1.80	A/B/C/D	74-198	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q63042.

Genome annotation databases

Ensembl

ENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370; Rattus norvegicus. [Genome view]

GeneID

27100.

KEGG

rno:27100.

UCSC

NM_013222. rat.

Organism-specific databases

CTD

27100.

RGD

61845. Gfer.

Phylogenomic databases

eggNOG

roNOG12723.

HOVERGEN

Q63042.

InParanoid

Q63042.

OMA

CTDFKSW.

OrthoDB

EOG9JMB8W.

PhylomeDB

Q63042.

Enzyme and pathway databases

BRENDA

1.8.3.2. 248.

Gene expression databases

ArrayExpress

Q63042.

Genevestigator

Q63042.

GermOnline

ENSRNOG00000013370. Rattus norvegicus.

Family and domain databases

InterPro

IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006863. Evr1_Alr.
[Graphical view]

Gene3D

G3DSA:1.20.120.310. Evr1_Alr. 1 hit.

Pfam

PF04777. Evr1_Alr. 1 hit.
[Graphical view]

PROSITE

PS51324. ERV_ALR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

608049.

Entry information

Entry name

ALR_RAT

Accession

Primary (citable) accession number: Q63042

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 16, 2008
Last modified:	January 19, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)