Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104FAD2 Publications1
Binding sitei133FAD2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100FAD2 Publications9
Nucleotide bindingi164 – 176FAD2 PublicationsAdd BLAST13
Nucleotide bindingi187 – 188FAD2 Publications2

GO - Molecular functioni

GO - Biological processi

  • cellular response to actinomycin D Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to toxic substance Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • liver regeneration Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of natural killer cell mediated cytotoxicity Source: RGD
  • negative regulation of oxidative stress-induced neuron death Source: RGD
  • positive regulation of DNA biosynthetic process Source: RGD
  • response to lipopolysaccharide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
Alternative name(s):
Augmenter of liver regeneration
Gene namesi
Name:Gfer
Synonyms:Alr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61845. Gfer.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • extracellular space Source: RGD
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085501 – 198FAD-linked sulfhydryl oxidase ALRAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi88Interchain (with C-197)PROSITE-ProRule annotation1 Publication
Disulfide bondi135 ↔ 138Redox-activePROSITE-ProRule annotation1 Publication
Disulfide bondi164 ↔ 181PROSITE-ProRule annotation1 Publication
Disulfide bondi197Interchain (with C-88)PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ63042.
PRIDEiQ63042.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013370.
GenevisibleiQ63042. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017917.

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi92 – 108Combined sources17
Helixi116 – 132Combined sources17
Helixi136 – 148Combined sources13
Helixi156 – 173Combined sources18
Helixi181 – 183Combined sources3
Helixi184 – 188Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQCX-ray1.80A/B/C/D74-198[»]
3R7CX-ray2.40A/B/C/D74-198[»]
SMRiQ63042.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63042.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 188ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiQ63042.
KOiK17783.
OMAiHGGNLFF.
OrthoDBiEOG091G0TRW.
PhylomeDBiQ63042.
TreeFamiTF105271.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q63042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA
60 70 80 90 100
APAPKGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW
110 120 130 140 150
AFLHTLAAYY PDMPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Length:198
Mass (Da):22,837
Last modified:December 16, 2008 - v2
Checksum:i46AD9BEC2EF0C393
GO

Sequence cautioni

The sequence AAF12808 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA06399 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30735 mRNA. Translation: BAA06399.1. Different initiation.
AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.
PIRiI80184.
RefSeqiNP_037354.2. NM_013222.2.
UniGeneiRn.11039.

Genome annotation databases

EnsembliENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370.
GeneIDi27100.
KEGGirno:27100.
UCSCiRGD:61845. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30735 mRNA. Translation: BAA06399.1. Different initiation.
AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.
PIRiI80184.
RefSeqiNP_037354.2. NM_013222.2.
UniGeneiRn.11039.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQCX-ray1.80A/B/C/D74-198[»]
3R7CX-ray2.40A/B/C/D74-198[»]
SMRiQ63042.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017917.

Proteomic databases

PaxDbiQ63042.
PRIDEiQ63042.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370.
GeneIDi27100.
KEGGirno:27100.
UCSCiRGD:61845. rat.

Organism-specific databases

CTDi2671.
RGDi61845. Gfer.

Phylogenomic databases

eggNOGiKOG3355. Eukaryota.
COG5054. LUCA.
GeneTreeiENSGT00390000001979.
HOGENOMiHOG000195924.
HOVERGENiHBG000235.
InParanoidiQ63042.
KOiK17783.
OMAiHGGNLFF.
OrthoDBiEOG091G0TRW.
PhylomeDBiQ63042.
TreeFamiTF105271.

Enzyme and pathway databases

BRENDAi1.8.3.2. 5301.

Miscellaneous databases

EvolutionaryTraceiQ63042.
PROiQ63042.

Gene expression databases

BgeeiENSRNOG00000013370.
GenevisibleiQ63042. RN.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALR_RAT
AccessioniPrimary (citable) accession number: Q63042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.