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Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104FAD2 Publications1
Binding sitei133FAD2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 100FAD2 Publications9
Nucleotide bindingi164 – 176FAD2 PublicationsAdd BLAST13
Nucleotide bindingi187 – 188FAD2 Publications2

GO - Molecular functioni

GO - Biological processi

  • cellular response to actinomycin D Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to toxic substance Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • liver regeneration Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of natural killer cell mediated cytotoxicity Source: RGD
  • negative regulation of oxidative stress-induced neuron death Source: RGD
  • positive regulation of DNA biosynthetic process Source: RGD
  • response to lipopolysaccharide Source: RGD

Keywordsi

Molecular functionGrowth factor, Oxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2 5301

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
Alternative name(s):
Augmenter of liver regeneration
Gene namesi
Name:Gfer
Synonyms:Alr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi61845 Gfer

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085501 – 198FAD-linked sulfhydryl oxidase ALRAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi88Interchain (with C-197)PROSITE-ProRule annotation1 Publication
Disulfide bondi135 ↔ 138Redox-activePROSITE-ProRule annotation1 Publication
Disulfide bondi164 ↔ 181PROSITE-ProRule annotation1 Publication
Disulfide bondi197Interchain (with C-88)PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ63042
PRIDEiQ63042

Expressioni

Gene expression databases

BgeeiENSRNOG00000013370
GenevisibleiQ63042 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked (PubMed:12717032, PubMed:22948913). Interacts with CHCHD4/MIA40 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017917

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi92 – 108Combined sources17
Helixi116 – 132Combined sources17
Helixi136 – 148Combined sources13
Helixi156 – 173Combined sources18
Helixi181 – 183Combined sources3
Helixi184 – 188Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQCX-ray1.80A/B/C/D74-198[»]
3R7CX-ray2.40A/B/C/D74-198[»]
SMRiQ63042
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63042

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 188ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST101

Phylogenomic databases

eggNOGiKOG3355 Eukaryota
COG5054 LUCA
GeneTreeiENSGT00390000001979
HOGENOMiHOG000195924
HOVERGENiHBG000235
InParanoidiQ63042
KOiK17783
OMAiCWVCAED
OrthoDBiEOG091G0TRW
PhylomeDBiQ63042
TreeFamiTF105271

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

Q63042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA
60 70 80 90 100
APAPKGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW
110 120 130 140 150
AFLHTLAAYY PDMPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Length:198
Mass (Da):22,837
Last modified:December 16, 2008 - v2
Checksum:i46AD9BEC2EF0C393
GO

Sequence cautioni

The sequence AAF12808 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA06399 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30735 mRNA Translation: BAA06399.1 Different initiation.
AF197192 Genomic DNA Translation: AAF12808.1 Different initiation.
PIRiI80184
RefSeqiNP_037354.2, NM_013222.2
UniGeneiRn.11039

Genome annotation databases

EnsembliENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370
GeneIDi27100
KEGGirno:27100
UCSCiRGD:61845 rat

Similar proteinsi

Entry informationi

Entry nameiALR_RAT
AccessioniPrimary (citable) accession number: Q63042
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 16, 2008
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health