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Q63042

- ALR_RAT

UniProt

Q63042 - ALR_RAT

Protein

FAD-linked sulfhydryl oxidase ALR

Gene

Gfer

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.1 Publication

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    FAD.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041FAD2 Publications
    Binding sitei133 – 1331FAD2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi92 – 1009FAD2 Publications
    Nucleotide bindingi164 – 17613FAD2 PublicationsAdd
    BLAST
    Nucleotide bindingi187 – 1882FAD2 Publications

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: UniProtKB
    2. protein disulfide oxidoreductase activity Source: UniProtKB
    3. thiol oxidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Growth factor, Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.8.3.2. 5301.
    ReactomeiREACT_199211. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)
    Alternative name(s):
    Augmenter of liver regeneration
    Gene namesi
    Name:Gfer
    Synonyms:Alr
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi61845. Gfer.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198FAD-linked sulfhydryl oxidase ALRPRO_0000208550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi88 – 88Interchain (with C-197)1 PublicationPROSITE-ProRule annotation
    Disulfide bondi135 ↔ 138Redox-active1 PublicationPROSITE-ProRule annotation
    Disulfide bondi164 ↔ 1811 PublicationPROSITE-ProRule annotation
    Disulfide bondi197 – 197Interchain (with C-88)1 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ63042.
    PRIDEiQ63042.

    Expressioni

    Gene expression databases

    GenevestigatoriQ63042.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.2 Publications

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000017917.

    Structurei

    Secondary structure

    1
    198
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi92 – 10817
    Helixi116 – 13217
    Helixi136 – 14813
    Helixi156 – 17318
    Helixi181 – 1833
    Helixi184 – 1885

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OQCX-ray1.80A/B/C/D74-198[»]
    3R7CX-ray2.40A/B/C/D74-198[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ63042.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini88 – 188101ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5054.
    GeneTreeiENSGT00390000001979.
    HOGENOMiHOG000195924.
    HOVERGENiHBG000235.
    InParanoidiQ63042.
    KOiK17783.
    OMAiQKRDSKF.
    OrthoDBiEOG7HMS2X.
    PhylomeDBiQ63042.
    TreeFamiTF105271.

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view]
    PfamiPF04777. Evr1_Alr. 1 hit.
    [Graphical view]
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q63042-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA    50
    APAPKGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW 100
    AFLHTLAAYY PDMPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ 150
    PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD 198
    Length:198
    Mass (Da):22,837
    Last modified:December 16, 2008 - v2
    Checksum:i46AD9BEC2EF0C393
    GO

    Sequence cautioni

    The sequence AAF12808.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA06399.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30735 mRNA. Translation: BAA06399.1. Different initiation.
    AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.
    PIRiI80184.
    RefSeqiNP_037354.2. NM_013222.2.
    UniGeneiRn.11039.

    Genome annotation databases

    EnsembliENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370.
    GeneIDi27100.
    KEGGirno:27100.
    UCSCiRGD:61845. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30735 mRNA. Translation: BAA06399.1 . Different initiation.
    AF197192 Genomic DNA. Translation: AAF12808.1 . Different initiation.
    PIRi I80184.
    RefSeqi NP_037354.2. NM_013222.2.
    UniGenei Rn.11039.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OQC X-ray 1.80 A/B/C/D 74-198 [» ]
    3R7C X-ray 2.40 A/B/C/D 74-198 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000017917.

    Proteomic databases

    PaxDbi Q63042.
    PRIDEi Q63042.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017917 ; ENSRNOP00000017917 ; ENSRNOG00000013370 .
    GeneIDi 27100.
    KEGGi rno:27100.
    UCSCi RGD:61845. rat.

    Organism-specific databases

    CTDi 2671.
    RGDi 61845. Gfer.

    Phylogenomic databases

    eggNOGi COG5054.
    GeneTreei ENSGT00390000001979.
    HOGENOMi HOG000195924.
    HOVERGENi HBG000235.
    InParanoidi Q63042.
    KOi K17783.
    OMAi QKRDSKF.
    OrthoDBi EOG7HMS2X.
    PhylomeDBi Q63042.
    TreeFami TF105271.

    Enzyme and pathway databases

    BRENDAi 1.8.3.2. 5301.
    Reactomei REACT_199211. Mitochondrial protein import.

    Miscellaneous databases

    EvolutionaryTracei Q63042.
    NextBioi 608049.
    PROi Q63042.

    Gene expression databases

    Genevestigatori Q63042.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view ]
    Pfami PF04777. Evr1_Alr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution."
      Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E.
      Proc. Natl. Acad. Sci. U.S.A. 91:8142-8146(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN LIVER REGENERATION.
      Strain: Fischer 344.
      Tissue: Liver.
    2. "Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat."
      Dong J., Cheng J., Wang Q., Shi S., Wang G., Si C.
      Chin. Med. Sci. J. 17:63-67(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Wistar.
    3. "The crystal structure of augmenter of liver regeneration: a mammalian FAD-dependent sulfhydryl oxidase."
      Wu C.-K., Dailey T.A., Dailey H.A., Wang B.-C., Rose J.P.
      Protein Sci. 12:1109-1118(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH FAD, SUBUNIT, COFACTOR, DISULFIDE BONDS.
    4. "The structure of augmenter of liver regeneration crystallized in the presence of 50 mM CdCl2 reveals a novel Cd2Cl4O6 cluster that aids in crystal packing."
      Florence Q., Wu C.K., Habel J., Swindell J.T. II, Wang B.C., Rose J.P.
      Acta Crystallogr. D 68:1128-1133(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 74-198 IN COMPLEX WITH FAD, SUBUNIT.

    Entry informationi

    Entry nameiALR_RAT
    AccessioniPrimary (citable) accession number: Q63042
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3