FAD-linked sulfhydryl oxidase ALR - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

Q63042 (ALR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
FAD-linked sulfhydryl oxidase ALR
EC=1.8.3.2

Alternative name(s):
Augmenter of liver regeneration

Gene names

Name:	Gfer
Synonyms:	Alr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs. May have a function in liver regeneration and spermatogenesis. Ref.1
Catalytic activity	2 R'C(R)SH + O₂ = R'C(R)S-S(R)CR' + H₂O₂.
Cofactor	FAD. Ref.4
Subunit structure	Homodimer; disulfide-linked. Ref.4
Subcellular location	Cytoplasm. Mitochondrion intermembrane space By similarity.
Sequence similarities	Contains 1 ERV/ALR sulfhydryl oxidase domain.
Sequence caution	The sequence AAF12808.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA06399.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Growth factor Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

FAD-linked sulfhydryl oxidase ALR

PRO_0000208550

Regions

Domain

88 – 188

101

ERV/ALR sulfhydryl oxidase

Region

171 – 188

FAD-binding

Sites

Binding site

FAD By similarity

Binding site

FAD By similarity

Binding site

FAD By similarity

Binding site

164

FAD By similarity

Binding site

167

FAD By similarity

Binding site

173

FAD By similarity

Binding site

187

FAD By similarity

Amino acid modifications

Disulfide bond

Interchain (with C-197) Ref.4

Disulfide bond

135 ↔ 138

Redox-active Potential

Disulfide bond

164 ↔ 181

Ref.4

Disulfide bond

197

Interchain (with C-88) Ref.4

Secondary structure

198

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q63042 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 46AD9BEC2EF0C393
Show »

FASTA

198

22,837

        10         20         30         40         50         60 
MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA APAPKGLEHG 

        70         80         90        100        110        120 
KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW AFLHTLAAYY PDMPTPEQQQ 

       130        140        150        160        170        180 
DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD 

       190 
CSRVDERWRD GWKDGSCD

« Hide

References

[1]	"Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution." Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E. Proc. Natl. Acad. Sci. U.S.A. 91:8142-8146(1994) [PubMed: 8058770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN LIVER REGENERATION. Strain: Fischer 344. Tissue: Liver.
[2]	Erratum Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E. Proc. Natl. Acad. Sci. U.S.A. 92:3076-3076(1995) [PubMed: 7708779] [Abstract]
[3]	"Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat." Dong J., Cheng J., Wang Q., Shi S., Wang G., Si C. Chin. Med. Sci. J. 17:63-67(2002) [PubMed: 12906155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
[4]	"The crystal structure of augmenter of liver regeneration: a mammalian FAD-dependent sulfhydryl oxidase." Wu C.-K., Dailey T.A., Dailey H.A., Wang B.-C., Rose J.P. Protein Sci. 12:1109-1118(2003) [PubMed: 12717032] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), SUBUNIT, COFACTOR, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D30735 mRNA. Translation: BAA06399.1. Different initiation.
AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.

IPI

IPI00326141.

PIR

I80184.

RefSeq

NP_037354.2. NM_013222.2.

UniGene

Rn.11039.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OQC	X-ray	1.80	A/B/C/D	74-198	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q63042.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370.

GeneID

27100.

KEGG

rno:27100.

UCSC

NM_013222. rat.

Organism-specific databases

CTD

2671.

RGD

61845. Gfer.

Phylogenomic databases

eggNOG

roNOG12723.

GeneTree

ENSGT00390000001979.

HOVERGEN

HBG000235.

InParanoid

Q63042.

OMA

CTDFKSW.

OrthoDB

EOG4M91ST.

PhylomeDB

Q63042.

Enzyme and pathway databases

BRENDA

1.8.3.2. 5301.

Gene expression databases

ArrayExpress

Q63042.

Genevestigator

Q63042.

GermOnline

ENSRNOG00000013370. Rattus norvegicus.

Family and domain databases

InterPro

IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006863. Evr1_Alr.
[Graphical view]

Gene3D

G3DSA:1.20.120.310. Evr1_Alr. 1 hit.

Pfam

PF04777. Evr1_Alr. 1 hit.
[Graphical view]

SUPFAM

SSF69000. Evr1_Alr. 1 hit.

PROSITE

PS51324. ERV_ALR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

608049.

Entry information

Entry name

ALR_RAT

Accession

Primary (citable) accession number: Q63042

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 16, 2008
Last modified:	November 16, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents