Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q63042 (ALR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FAD-linked sulfhydryl oxidase ALR

EC=1.8.3.2
Alternative name(s):
Augmenter of liver regeneration
Gene names
Name:Gfer
Synonyms:Alr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis. Ref.1

Catalytic activity

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactor

FAD. Ref.4

Subunit structure

Homodimer; disulfide-linked. Ref.4 Ref.5

Subcellular location

Cytoplasm. Mitochondrion intermembrane space By similarity.

Sequence similarities

Contains 1 ERV/ALR sulfhydryl oxidase domain.

Sequence caution

The sequence AAF12808.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA06399.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198FAD-linked sulfhydryl oxidase ALR
PRO_0000208550

Regions

Domain88 – 188101ERV/ALR sulfhydryl oxidase
Nucleotide binding92 – 1009FAD
Nucleotide binding164 – 17613FAD
Nucleotide binding187 – 1882FAD

Sites

Binding site1041FAD
Binding site1331FAD

Amino acid modifications

Disulfide bond88Interchain (with C-197) Ref.4
Disulfide bond135 ↔ 138Redox-active Ref.4
Disulfide bond164 ↔ 181 Ref.4
Disulfide bond197Interchain (with C-88) Ref.4

Secondary structure

............ 198
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q63042 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 46AD9BEC2EF0C393

FASTA19822,837
        10         20         30         40         50         60 
MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA APAPKGLEHG 

        70         80         90        100        110        120 
KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW AFLHTLAAYY PDMPTPEQQQ 

       130        140        150        160        170        180 
DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD 

       190 
CSRVDERWRD GWKDGSCD 

« Hide

References

[1]"Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution."
Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E.
Proc. Natl. Acad. Sci. U.S.A. 91:8142-8146(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN LIVER REGENERATION.
Strain: Fischer 344.
Tissue: Liver.
[2]Erratum
Hagiya M., Francavilla A., Polimeno L., Ihara I., Sakai H., Seki T., Shimonishi M., Porter K.A., Starzl T.E.
Proc. Natl. Acad. Sci. U.S.A. 92:3076-3076(1995) [PubMed] [Europe PMC] [Abstract]
[3]"Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat."
Dong J., Cheng J., Wang Q., Shi S., Wang G., Si C.
Chin. Med. Sci. J. 17:63-67(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Wistar.
[4]"The crystal structure of augmenter of liver regeneration: a mammalian FAD-dependent sulfhydryl oxidase."
Wu C.-K., Dailey T.A., Dailey H.A., Wang B.-C., Rose J.P.
Protein Sci. 12:1109-1118(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH FAD, SUBUNIT, COFACTOR, DISULFIDE BONDS.
[5]"The structure of augmenter of liver regeneration crystallized in the presence of 50 mM CdCl2 reveals a novel Cd2Cl4O6 cluster that aids in crystal packing."
Florence Q., Wu C.K., Habel J., Swindell J.T. II, Wang B.C., Rose J.P.
Acta Crystallogr. D 68:1128-1133(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 74-198 IN COMPLEX WITH FAD, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D30735 mRNA. Translation: BAA06399.1. Different initiation.
AF197192 Genomic DNA. Translation: AAF12808.1. Different initiation.
PIRI80184.
RefSeqNP_037354.2. NM_013222.2.
UniGeneRn.11039.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OQCX-ray1.80A/B/C/D74-198[»]
3R7CX-ray2.40A/B/C/D74-198[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000017917.

Proteomic databases

PaxDbQ63042.
PRIDEQ63042.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370.
GeneID27100.
KEGGrno:27100.
UCSCRGD:61845. rat.

Organism-specific databases

CTD2671.
RGD61845. Gfer.

Phylogenomic databases

eggNOGCOG5054.
GeneTreeENSGT00390000001979.
HOGENOMHOG000195924.
HOVERGENHBG000235.
InParanoidQ63042.
KOK17783.
OMAQKRDSKF.
OrthoDBEOG7HMS2X.
PhylomeDBQ63042.
TreeFamTF105271.

Enzyme and pathway databases

BRENDA1.8.3.2. 5301.

Gene expression databases

GenevestigatorQ63042.

Family and domain databases

Gene3D1.20.120.310. 1 hit.
InterProIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMSSF69000. SSF69000. 1 hit.
PROSITEPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ63042.
NextBio608049.
PROQ63042.

Entry information

Entry nameALR_RAT
AccessionPrimary (citable) accession number: Q63042
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references