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Protein

Alpha-1-macroglobulin

Gene

A1m

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).By similarity

GO - Molecular functioni

  • brain-derived neurotrophic factor binding Source: RGD
  • nerve growth factor binding Source: RGD
  • protein complex binding Source: RGD
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI39.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1-macroglobulin
Short name:
Alpha-1-M
Alternative name(s):
Alpha-1-macroglobulin 165 kDa subunit
Cleaved into the following chain:
Gene namesi
Name:A1mImported
Synonyms:PzpImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi628643. Pzp.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000027140325 – 1500Alpha-1-macroglobulinAdd BLAST1476
ChainiPRO_00002714041245 – 1500Alpha-1-macroglobulin 45 kDa subunit1 PublicationAdd BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi48 ↔ 86By similarity
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Glycosylationi157N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi249 ↔ 298By similarity
Disulfide bondi267 ↔ 286By similarity
Disulfide bondi277Interchain (with C-430)By similarity
Glycosylationi382N-linked (GlcNAc...)Sequence analysis1
Glycosylationi412N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi430Interchain (with C-277)By similarity
Disulfide bondi469 ↔ 562By similarity
Glycosylationi568N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi594 ↔ 785By similarity
Disulfide bondi642 ↔ 689By similarity
Disulfide bondi835 ↔ 863By similarity
Disulfide bondi861 ↔ 897By similarity
Glycosylationi883N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi935 ↔ 1344By similarity
Glycosylationi944N-linked (GlcNAc...)Sequence analysis1
Cross-linki986 ↔ 989Isoglutamyl cysteine thioester (Cys-Gln)By similarity
Glycosylationi1005N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi1094 ↔ 1142By similarity
Glycosylationi1390N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1448N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

PaxDbiQ63041.
PRIDEiQ63041.

Expressioni

Tissue specificityi

Widely expressed. Highest level in ovary, testis, uterus and prostate. Protein found in plasma.1 Publication

Gene expression databases

BgeeiENSRNOG00000006709.
GenevisibleiQ63041. RN.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked.By similarity

GO - Molecular functioni

  • brain-derived neurotrophic factor binding Source: RGD
  • nerve growth factor binding Source: RGD
  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiQ63041. 1 interactor.
STRINGi10116.ENSRNOP00000009467.

Structurei

Secondary structure

11500
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1364 – 1375Combined sources12
Turni1376 – 1378Combined sources3
Beta strandi1384 – 1393Combined sources10
Beta strandi1395 – 1399Combined sources5
Beta strandi1401 – 1408Combined sources8
Beta strandi1413 – 1415Combined sources3
Helixi1417 – 1421Combined sources5
Helixi1422 – 1425Combined sources4
Beta strandi1429 – 1435Combined sources7
Beta strandi1438 – 1444Combined sources7
Beta strandi1451 – 1461Combined sources11
Beta strandi1469 – 1477Combined sources9
Beta strandi1481 – 1487Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDYX-ray2.30A/B1362-1495[»]
ProteinModelPortaliQ63041.
SMRiQ63041.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63041.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni686 – 746Bait regionBy similarityAdd BLAST61
Regioni1360 – 1500Receptor-binding domain1 PublicationAdd BLAST141

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiENOG410KCRJ. Eukaryota.
COG2373. LUCA.
GeneTreeiENSGT00760000118982.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiQ63041.
OMAiTHITNAF.
OrthoDBiEOG091G00F5.
PhylomeDBiQ63041.
TreeFamiTF313285.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SMARTiSM01360. A2M. 1 hit.
SM01359. A2M_N_2. 1 hit.
SM01361. A2M_recep. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNQLPIPV FLLLLLLLPR DATAATGKPR YVVLVPSELY AGVPEKVCVH
60 70 80 90 100
LNHLNETVTL NVTLEYGVQY SNLLIDQAVD KDSSYCSSFT ISRPLSPSAL
110 120 130 140 150
IAVEIKGPTH HFIKKKSMWI TKAESPVFVQ TDKPIYKPGQ TVKFRVVSVD
160 170 180 190 200
ISFRPVNETF PVVYIENPKR NRIFQWQNVD LPGGLHQLSF PLSVEPALGI
210 220 230 240 250
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVQ VKMPKTMAFL EEELVVTACG
260 270 280 290 300
LYTYGKPVPG LVTMKVCRKY TQSYSNCHGQ HSKSICEEFS KQADEKGCFR
310 320 330 340 350
QVVKTKVFQP RQKGYDMKIE VEAKIKEDGT GIELTGTGSC EIANTLSKLK
360 370 380 390 400
FTKANTFYRP GLPFFGQVLL VDEKGQPIPN KNLTVQVNSV RSQFTFTTDE
410 420 430 440 450
HGLANILIDT TNFTFSFMGI RVIYKQNNIC FDNWWVDEYH TQADHSAARI
460 470 480 490 500
FSPSRSYIQL ELVLGTLACG QTQEIRIHFL LNEDALKDAK DLTFYYLIKA
510 520 530 540 550
RGSIFNSGSH VLPLEQGKVK GVVSFPIRVE PGMAPVAKLI VYTILPNEEL
560 570 580 590 600
IADVQKFDIE KCFANTVNLS FPSAQSLPAS DTHLTVKATP LSLCALTAVD
610 620 630 640 650
QSVLLLKPEA KLSPQSIYNL LPQKAEQGAY LGPLPYKGGE NCIKAEDITH
660 670 680 690 700
NGIVYTPKQD LNDNDAYSVF QSIGLKIFTN TRVHKPRYCP MYQAYPPLPY
710 720 730 740 750
VGEPQALAMS AIPGAGYRSS NIRTSSMMMM GASEVAQEVE VRETVRKYFP
760 770 780 790 800
ETWIWDMVPL DLSGDGELPV KVPDTITEWK ASAFCLSGTT GLGLSSTISH
810 820 830 840 850
KVFQPFFLEL TLPYSVVRGE AFILKATVLN YMPHCIRIHV SLEMSPDFLA
860 870 880 890 900
VPVGSHEDSH CICGNERKTV SWAVTPKSLG EVNFTATAEA LQSPELCGNK
910 920 930 940 950
VAEVPALVQK DTVVKPVIVE PEGIEKEQTY NTLLCPQDAE LQENWTLDLP
960 970 980 990 1000
ANVVEGSARA TQSVLGDILG SAMQNLQNLL QMPYGCGEQN MVLFVPNIYV
1010 1020 1030 1040 1050
LEYLNETQQL TEAIKSKAIS YLISGYQRQL NYQHSDGSYS TFGDRGMRHS
1060 1070 1080 1090 1100
QGNTWLTAFV LKAFAQAQSY IYIEKTHITN AFNWLSMKQR ENGCFQQSGS
1110 1120 1130 1140 1150
LLNNAMKGGV DDEVTLSAYI TIALLEMPLP VTHSVVRNAL FCLETAWASI
1160 1170 1180 1190 1200
SNSQESHVYT KALLAYAFAL AGNRAKRSEV LESLNKDAVN EEESVHWQRP
1210 1220 1230 1240 1250
KNVEENVREM RSFSYKPRAP SAEVEMTAYV LLAYLTSASS RPTRDLSSSD
1260 1270 1280 1290 1300
LTTASKIVKW ISKQQNSHGG FSSTQDTVVA LQALSKYGAA TFTKSNKEVS
1310 1320 1330 1340 1350
VTIESSGTVS GTLHVNNGNR LLLQEVRLAD LPGNYITKVS GSGCVYLQTS
1360 1370 1380 1390 1400
LKYNILPEAE GEAPFTLKVN TLPLNFDKAE HHRKFQIHIN VSYIGERPNS
1410 1420 1430 1440 1450
NMVIVDVKMV SGFIPVKPSV KKLQDQSNIQ RTEVNTNHVL IYIEKLTNQT
1460 1470 1480 1490 1500
MGFSFAVEQD IPVKNLKPAP VKVYDYYETD EFAIEEYSAP FSSDSEQGNA
Length:1,500
Mass (Da):167,125
Last modified:November 1, 1996 - v1
Checksum:i8ABB810985795AB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50H → A AA sequence (PubMed:1725450).Curated1
Sequence conflicti918I → P AA sequence (PubMed:1725450).Curated1
Sequence conflicti924 – 925IE → AT AA sequence (PubMed:1725450).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77183 mRNA. Translation: AAA40723.1.
M84000 mRNA. Translation: AAA41591.1.
PIRiA42210.
RefSeqiNP_665722.2. NM_145779.2.
UniGeneiRn.11295.

Genome annotation databases

EnsembliENSRNOT00000009467; ENSRNOP00000009467; ENSRNOG00000006709.
GeneIDi252922.
KEGGirno:252922.
UCSCiRGD:628643. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77183 mRNA. Translation: AAA40723.1.
M84000 mRNA. Translation: AAA41591.1.
PIRiA42210.
RefSeqiNP_665722.2. NM_145779.2.
UniGeneiRn.11295.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDYX-ray2.30A/B1362-1495[»]
ProteinModelPortaliQ63041.
SMRiQ63041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ63041. 1 interactor.
STRINGi10116.ENSRNOP00000009467.

Protein family/group databases

MEROPSiI39.003.

Proteomic databases

PaxDbiQ63041.
PRIDEiQ63041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009467; ENSRNOP00000009467; ENSRNOG00000006709.
GeneIDi252922.
KEGGirno:252922.
UCSCiRGD:628643. rat.

Organism-specific databases

CTDi5858.
RGDi628643. Pzp.

Phylogenomic databases

eggNOGiENOG410KCRJ. Eukaryota.
COG2373. LUCA.
GeneTreeiENSGT00760000118982.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiQ63041.
OMAiTHITNAF.
OrthoDBiEOG091G00F5.
PhylomeDBiQ63041.
TreeFamiTF313285.

Miscellaneous databases

EvolutionaryTraceiQ63041.
PROiQ63041.

Gene expression databases

BgeeiENSRNOG00000006709.
GenevisibleiQ63041. RN.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SMARTiSM01360. A2M. 1 hit.
SM01359. A2M_N_2. 1 hit.
SM01361. A2M_recep. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA1M_RAT
AccessioniPrimary (citable) accession number: Q63041
Secondary accession number(s): Q63332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.