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Q63041

- A1M_RAT

UniProt

Q63041 - A1M_RAT

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Protein

Alpha-1-macroglobulin

Gene
A1m, Pzp
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase By similarity.By similarity

GO - Molecular functioni

  1. protein complex binding Source: RGD
  2. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. embryo implantation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1-macroglobulin
Short name:
Alpha-1-M
Alternative name(s):
Alpha-1-macroglobulin 165 kDa subunit
Cleaved into the following chain:
Gene namesi
Name:A1m
Synonyms:Pzp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi628643. Pzp.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 15001476Alpha-1-macroglobulin1 PublicationPRO_0000271403Add
BLAST
Chaini1245 – 1500256Alpha-1-macroglobulin 45 kDa subunit1 PublicationPRO_0000271404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 86 By similarityBy similarity
Glycosylationi55 – 551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi157 – 1571N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi249 ↔ 298 By similarityBy similarity
Disulfide bondi267 ↔ 286 By similarityBy similarity
Disulfide bondi277 – 277Interchain (with C-430) By similarityBy similarity
Glycosylationi382 – 3821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi412 – 4121N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi430 – 430Interchain (with C-277) By similarityBy similarity
Disulfide bondi469 ↔ 562 By similarityBy similarity
Glycosylationi568 – 5681N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi594 ↔ 785 By similarityBy similarity
Disulfide bondi642 ↔ 689 By similarityBy similarity
Disulfide bondi835 ↔ 863 By similarityBy similarity
Disulfide bondi861 ↔ 897 By similarityBy similarity
Glycosylationi883 – 8831N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi935 ↔ 1344 By similarityBy similarity
Glycosylationi944 – 9441N-linked (GlcNAc...) Reviewed prediction
Cross-linki986 ↔ 989Isoglutamyl cysteine thioester (Cys-Gln) By similarityBy similarity
Glycosylationi1005 – 10051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1094 ↔ 1142 By similarityBy similarity
Glycosylationi1390 – 13901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1448 – 14481N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

PaxDbiQ63041.
PRIDEiQ63041.

Expressioni

Tissue specificityi

Widely expressed. Highest level in ovary, testis, uterus and prostate. Protein found in plasma.1 Publication

Gene expression databases

GenevestigatoriQ63041.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked By similarity.By similarity

Protein-protein interaction databases

IntActiQ63041. 1 interaction.
STRINGi10116.ENSRNOP00000009467.

Structurei

Secondary structure

1
1500
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1364 – 137512
Turni1376 – 13783
Beta strandi1384 – 139310
Beta strandi1395 – 13995
Beta strandi1401 – 14088
Beta strandi1413 – 14153
Helixi1417 – 14215
Helixi1422 – 14254
Beta strandi1429 – 14357
Beta strandi1438 – 14447
Beta strandi1451 – 146111
Beta strandi1469 – 14779
Beta strandi1481 – 14877

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDYX-ray2.30A/B1362-1495[»]
ProteinModelPortaliQ63041.
SMRiQ63041. Positions 124-225, 1362-1495.

Miscellaneous databases

EvolutionaryTraceiQ63041.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni686 – 74661Bait region By similarityBy similarityAdd
BLAST
Regioni1360 – 1500141Receptor-binding domain1 PublicationAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiCOG2373.
GeneTreeiENSGT00740000115177.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiQ63041.
OMAiTHITNAF.
OrthoDBiEOG7DJSKB.
PhylomeDBiQ63041.
TreeFamiTF313285.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63041-1 [UniParc]FASTAAdd to Basket

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MRRNQLPIPV FLLLLLLLPR DATAATGKPR YVVLVPSELY AGVPEKVCVH     50
LNHLNETVTL NVTLEYGVQY SNLLIDQAVD KDSSYCSSFT ISRPLSPSAL 100
IAVEIKGPTH HFIKKKSMWI TKAESPVFVQ TDKPIYKPGQ TVKFRVVSVD 150
ISFRPVNETF PVVYIENPKR NRIFQWQNVD LPGGLHQLSF PLSVEPALGI 200
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVQ VKMPKTMAFL EEELVVTACG 250
LYTYGKPVPG LVTMKVCRKY TQSYSNCHGQ HSKSICEEFS KQADEKGCFR 300
QVVKTKVFQP RQKGYDMKIE VEAKIKEDGT GIELTGTGSC EIANTLSKLK 350
FTKANTFYRP GLPFFGQVLL VDEKGQPIPN KNLTVQVNSV RSQFTFTTDE 400
HGLANILIDT TNFTFSFMGI RVIYKQNNIC FDNWWVDEYH TQADHSAARI 450
FSPSRSYIQL ELVLGTLACG QTQEIRIHFL LNEDALKDAK DLTFYYLIKA 500
RGSIFNSGSH VLPLEQGKVK GVVSFPIRVE PGMAPVAKLI VYTILPNEEL 550
IADVQKFDIE KCFANTVNLS FPSAQSLPAS DTHLTVKATP LSLCALTAVD 600
QSVLLLKPEA KLSPQSIYNL LPQKAEQGAY LGPLPYKGGE NCIKAEDITH 650
NGIVYTPKQD LNDNDAYSVF QSIGLKIFTN TRVHKPRYCP MYQAYPPLPY 700
VGEPQALAMS AIPGAGYRSS NIRTSSMMMM GASEVAQEVE VRETVRKYFP 750
ETWIWDMVPL DLSGDGELPV KVPDTITEWK ASAFCLSGTT GLGLSSTISH 800
KVFQPFFLEL TLPYSVVRGE AFILKATVLN YMPHCIRIHV SLEMSPDFLA 850
VPVGSHEDSH CICGNERKTV SWAVTPKSLG EVNFTATAEA LQSPELCGNK 900
VAEVPALVQK DTVVKPVIVE PEGIEKEQTY NTLLCPQDAE LQENWTLDLP 950
ANVVEGSARA TQSVLGDILG SAMQNLQNLL QMPYGCGEQN MVLFVPNIYV 1000
LEYLNETQQL TEAIKSKAIS YLISGYQRQL NYQHSDGSYS TFGDRGMRHS 1050
QGNTWLTAFV LKAFAQAQSY IYIEKTHITN AFNWLSMKQR ENGCFQQSGS 1100
LLNNAMKGGV DDEVTLSAYI TIALLEMPLP VTHSVVRNAL FCLETAWASI 1150
SNSQESHVYT KALLAYAFAL AGNRAKRSEV LESLNKDAVN EEESVHWQRP 1200
KNVEENVREM RSFSYKPRAP SAEVEMTAYV LLAYLTSASS RPTRDLSSSD 1250
LTTASKIVKW ISKQQNSHGG FSSTQDTVVA LQALSKYGAA TFTKSNKEVS 1300
VTIESSGTVS GTLHVNNGNR LLLQEVRLAD LPGNYITKVS GSGCVYLQTS 1350
LKYNILPEAE GEAPFTLKVN TLPLNFDKAE HHRKFQIHIN VSYIGERPNS 1400
NMVIVDVKMV SGFIPVKPSV KKLQDQSNIQ RTEVNTNHVL IYIEKLTNQT 1450
MGFSFAVEQD IPVKNLKPAP VKVYDYYETD EFAIEEYSAP FSSDSEQGNA 1500
Length:1,500
Mass (Da):167,125
Last modified:November 1, 1996 - v1
Checksum:i8ABB810985795AB2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501H → A AA sequence 1 Publication
Sequence conflicti918 – 9181I → P AA sequence 1 Publication
Sequence conflicti924 – 9252IE → AT AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77183 mRNA. Translation: AAA40723.1.
M84000 mRNA. Translation: AAA41591.1.
PIRiA42210.
RefSeqiNP_665722.2. NM_145779.2.
UniGeneiRn.11295.

Genome annotation databases

EnsembliENSRNOT00000009467; ENSRNOP00000009467; ENSRNOG00000006709.
GeneIDi252922.
KEGGirno:252922.
UCSCiRGD:628643. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77183 mRNA. Translation: AAA40723.1 .
M84000 mRNA. Translation: AAA41591.1 .
PIRi A42210.
RefSeqi NP_665722.2. NM_145779.2.
UniGenei Rn.11295.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EDY X-ray 2.30 A/B 1362-1495 [» ]
ProteinModelPortali Q63041.
SMRi Q63041. Positions 124-225, 1362-1495.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63041. 1 interaction.
STRINGi 10116.ENSRNOP00000009467.

Proteomic databases

PaxDbi Q63041.
PRIDEi Q63041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009467 ; ENSRNOP00000009467 ; ENSRNOG00000006709 .
GeneIDi 252922.
KEGGi rno:252922.
UCSCi RGD:628643. rat.

Organism-specific databases

CTDi 5858.
RGDi 628643. Pzp.

Phylogenomic databases

eggNOGi COG2373.
GeneTreei ENSGT00740000115177.
HOGENOMi HOG000220939.
HOVERGENi HBG000039.
InParanoidi Q63041.
OMAi THITNAF.
OrthoDBi EOG7DJSKB.
PhylomeDBi Q63041.
TreeFami TF313285.

Miscellaneous databases

EvolutionaryTracei Q63041.
NextBioi 624079.

Gene expression databases

Genevestigatori Q63041.

Family and domain databases

Gene3Di 1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProi IPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of rat alpha 1-macroglobulin, a broad-range proteinase inhibitor from the alpha-macroglobulin-complement family."
    Eggertsen G., Hudson G., Shiels B., Reed D., Fey G.H.
    Mol. Biol. Med. 8:287-302(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-54; 724-743; 901-930 AND 1245-1269, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Fischer 344.
    Tissue: Liver.
  2. "cDNA cloning and sequencing of rat alpha 1-macroglobulin."
    Warmegard B., Martin N., Johansson S.
    Biochemistry 31:2346-2352(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1287-1294 AND 1321-1327, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  4. "Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer."
    Xiao T., DeCamp D.L., Spran S.R.
    Protein Sci. 9:1889-1897(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1362-1495.

Entry informationi

Entry nameiA1M_RAT
AccessioniPrimary (citable) accession number: Q63041
Secondary accession number(s): Q63332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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