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Q63041

- A1M_RAT

UniProt

Q63041 - A1M_RAT

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Protein

Alpha-1-macroglobulin

Gene

A1m

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase By similarity.By similarity

GO - Molecular functioni

  1. protein complex binding Source: RGD
  2. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. embryo implantation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI39.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1-macroglobulin
Short name:
Alpha-1-M
Alternative name(s):
Alpha-1-macroglobulin 165 kDa subunit
Cleaved into the following chain:
Gene namesi
Name:A1mImported
Synonyms:PzpImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi628643. Pzp.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 15001476Alpha-1-macroglobulinPRO_0000271403Add
BLAST
Chaini1245 – 1500256Alpha-1-macroglobulin 45 kDa subunit1 PublicationPRO_0000271404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 86By similarity
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi249 ↔ 298By similarity
Disulfide bondi267 ↔ 286By similarity
Disulfide bondi277 – 277Interchain (with C-430)By similarity
Glycosylationi382 – 3821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi430 – 430Interchain (with C-277)By similarity
Disulfide bondi469 ↔ 562By similarity
Glycosylationi568 – 5681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi594 ↔ 785By similarity
Disulfide bondi642 ↔ 689By similarity
Disulfide bondi835 ↔ 863By similarity
Disulfide bondi861 ↔ 897By similarity
Glycosylationi883 – 8831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi935 ↔ 1344By similarity
Glycosylationi944 – 9441N-linked (GlcNAc...)Sequence Analysis
Cross-linki986 ↔ 989Isoglutamyl cysteine thioester (Cys-Gln)By similarity
Glycosylationi1005 – 10051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi1094 ↔ 1142By similarity
Glycosylationi1390 – 13901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1448 – 14481N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

PaxDbiQ63041.
PRIDEiQ63041.

Expressioni

Tissue specificityi

Widely expressed. Highest level in ovary, testis, uterus and prostate. Protein found in plasma.1 Publication

Gene expression databases

GenevestigatoriQ63041.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked.By similarity

Protein-protein interaction databases

IntActiQ63041. 1 interaction.
STRINGi10116.ENSRNOP00000009467.

Structurei

Secondary structure

1
1500
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1364 – 137512
Turni1376 – 13783
Beta strandi1384 – 139310
Beta strandi1395 – 13995
Beta strandi1401 – 14088
Beta strandi1413 – 14153
Helixi1417 – 14215
Helixi1422 – 14254
Beta strandi1429 – 14357
Beta strandi1438 – 14447
Beta strandi1451 – 146111
Beta strandi1469 – 14779
Beta strandi1481 – 14877

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDYX-ray2.30A/B1362-1495[»]
ProteinModelPortaliQ63041.
SMRiQ63041. Positions 124-225, 1362-1495.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ63041.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni686 – 74661Bait regionBy similarityAdd
BLAST
Regioni1360 – 1500141Receptor-binding domain1 PublicationAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiCOG2373.
GeneTreeiENSGT00760000118982.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiQ63041.
OMAiTHITNAF.
OrthoDBiEOG7DJSKB.
PhylomeDBiQ63041.
TreeFamiTF313285.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63041-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRNQLPIPV FLLLLLLLPR DATAATGKPR YVVLVPSELY AGVPEKVCVH
60 70 80 90 100
LNHLNETVTL NVTLEYGVQY SNLLIDQAVD KDSSYCSSFT ISRPLSPSAL
110 120 130 140 150
IAVEIKGPTH HFIKKKSMWI TKAESPVFVQ TDKPIYKPGQ TVKFRVVSVD
160 170 180 190 200
ISFRPVNETF PVVYIENPKR NRIFQWQNVD LPGGLHQLSF PLSVEPALGI
210 220 230 240 250
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVQ VKMPKTMAFL EEELVVTACG
260 270 280 290 300
LYTYGKPVPG LVTMKVCRKY TQSYSNCHGQ HSKSICEEFS KQADEKGCFR
310 320 330 340 350
QVVKTKVFQP RQKGYDMKIE VEAKIKEDGT GIELTGTGSC EIANTLSKLK
360 370 380 390 400
FTKANTFYRP GLPFFGQVLL VDEKGQPIPN KNLTVQVNSV RSQFTFTTDE
410 420 430 440 450
HGLANILIDT TNFTFSFMGI RVIYKQNNIC FDNWWVDEYH TQADHSAARI
460 470 480 490 500
FSPSRSYIQL ELVLGTLACG QTQEIRIHFL LNEDALKDAK DLTFYYLIKA
510 520 530 540 550
RGSIFNSGSH VLPLEQGKVK GVVSFPIRVE PGMAPVAKLI VYTILPNEEL
560 570 580 590 600
IADVQKFDIE KCFANTVNLS FPSAQSLPAS DTHLTVKATP LSLCALTAVD
610 620 630 640 650
QSVLLLKPEA KLSPQSIYNL LPQKAEQGAY LGPLPYKGGE NCIKAEDITH
660 670 680 690 700
NGIVYTPKQD LNDNDAYSVF QSIGLKIFTN TRVHKPRYCP MYQAYPPLPY
710 720 730 740 750
VGEPQALAMS AIPGAGYRSS NIRTSSMMMM GASEVAQEVE VRETVRKYFP
760 770 780 790 800
ETWIWDMVPL DLSGDGELPV KVPDTITEWK ASAFCLSGTT GLGLSSTISH
810 820 830 840 850
KVFQPFFLEL TLPYSVVRGE AFILKATVLN YMPHCIRIHV SLEMSPDFLA
860 870 880 890 900
VPVGSHEDSH CICGNERKTV SWAVTPKSLG EVNFTATAEA LQSPELCGNK
910 920 930 940 950
VAEVPALVQK DTVVKPVIVE PEGIEKEQTY NTLLCPQDAE LQENWTLDLP
960 970 980 990 1000
ANVVEGSARA TQSVLGDILG SAMQNLQNLL QMPYGCGEQN MVLFVPNIYV
1010 1020 1030 1040 1050
LEYLNETQQL TEAIKSKAIS YLISGYQRQL NYQHSDGSYS TFGDRGMRHS
1060 1070 1080 1090 1100
QGNTWLTAFV LKAFAQAQSY IYIEKTHITN AFNWLSMKQR ENGCFQQSGS
1110 1120 1130 1140 1150
LLNNAMKGGV DDEVTLSAYI TIALLEMPLP VTHSVVRNAL FCLETAWASI
1160 1170 1180 1190 1200
SNSQESHVYT KALLAYAFAL AGNRAKRSEV LESLNKDAVN EEESVHWQRP
1210 1220 1230 1240 1250
KNVEENVREM RSFSYKPRAP SAEVEMTAYV LLAYLTSASS RPTRDLSSSD
1260 1270 1280 1290 1300
LTTASKIVKW ISKQQNSHGG FSSTQDTVVA LQALSKYGAA TFTKSNKEVS
1310 1320 1330 1340 1350
VTIESSGTVS GTLHVNNGNR LLLQEVRLAD LPGNYITKVS GSGCVYLQTS
1360 1370 1380 1390 1400
LKYNILPEAE GEAPFTLKVN TLPLNFDKAE HHRKFQIHIN VSYIGERPNS
1410 1420 1430 1440 1450
NMVIVDVKMV SGFIPVKPSV KKLQDQSNIQ RTEVNTNHVL IYIEKLTNQT
1460 1470 1480 1490 1500
MGFSFAVEQD IPVKNLKPAP VKVYDYYETD EFAIEEYSAP FSSDSEQGNA
Length:1,500
Mass (Da):167,125
Last modified:November 1, 1996 - v1
Checksum:i8ABB810985795AB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501H → A AA sequence (PubMed:1725450)Curated
Sequence conflicti918 – 9181I → P AA sequence (PubMed:1725450)Curated
Sequence conflicti924 – 9252IE → AT AA sequence (PubMed:1725450)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77183 mRNA. Translation: AAA40723.1.
M84000 mRNA. Translation: AAA41591.1.
PIRiA42210.
RefSeqiNP_665722.2. NM_145779.2.
UniGeneiRn.11295.

Genome annotation databases

EnsembliENSRNOT00000009467; ENSRNOP00000009467; ENSRNOG00000006709.
GeneIDi252922.
KEGGirno:252922.
UCSCiRGD:628643. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M77183 mRNA. Translation: AAA40723.1 .
M84000 mRNA. Translation: AAA41591.1 .
PIRi A42210.
RefSeqi NP_665722.2. NM_145779.2.
UniGenei Rn.11295.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EDY X-ray 2.30 A/B 1362-1495 [» ]
ProteinModelPortali Q63041.
SMRi Q63041. Positions 124-225, 1362-1495.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63041. 1 interaction.
STRINGi 10116.ENSRNOP00000009467.

Protein family/group databases

MEROPSi I39.003.

Proteomic databases

PaxDbi Q63041.
PRIDEi Q63041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009467 ; ENSRNOP00000009467 ; ENSRNOG00000006709 .
GeneIDi 252922.
KEGGi rno:252922.
UCSCi RGD:628643. rat.

Organism-specific databases

CTDi 5858.
RGDi 628643. Pzp.

Phylogenomic databases

eggNOGi COG2373.
GeneTreei ENSGT00760000118982.
HOGENOMi HOG000220939.
HOVERGENi HBG000039.
InParanoidi Q63041.
OMAi THITNAF.
OrthoDBi EOG7DJSKB.
PhylomeDBi Q63041.
TreeFami TF313285.

Miscellaneous databases

EvolutionaryTracei Q63041.
NextBioi 624079.

Gene expression databases

Genevestigatori Q63041.

Family and domain databases

Gene3Di 1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProi IPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of rat alpha 1-macroglobulin, a broad-range proteinase inhibitor from the alpha-macroglobulin-complement family."
    Eggertsen G., Hudson G., Shiels B., Reed D., Fey G.H.
    Mol. Biol. Med. 8:287-302(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-54; 724-743; 901-930 AND 1245-1269, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Fischer 344Imported.
    Tissue: LiverImported.
  2. "cDNA cloning and sequencing of rat alpha 1-macroglobulin."
    Warmegard B., Martin N., Johansson S.
    Biochemistry 31:2346-2352(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-DawleyImported.
    Tissue: LiverImported.
  3. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1287-1294 AND 1321-1327, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  4. "Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer."
    Xiao T., DeCamp D.L., Spran S.R.
    Protein Sci. 9:1889-1897(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1362-1495.

Entry informationi

Entry nameiA1M_RAT
AccessioniPrimary (citable) accession number: Q63041
Secondary accession number(s): Q63332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3