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Q63041 (A1M_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1-macroglobulin
Short name=Alpha-1-M
Alternative name(s):
Alpha-1-macroglobulin 165 kDa subunit

Cleaved into the following chain:

  1. Alpha-1-macroglobulin 45 kDa subunit
Gene names
Name:A1m
Synonyms:Pzp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase By similarity. UniProtKB P01023

Subunit structure

Homotetramer; disulfide-linked By similarity. UniProtKB P01023

Subcellular location

Secreted Ref.1.

Tissue specificity

Widely expressed. Highest level in ovary, testis, uterus and prostate. Protein found in plasma. Ref.1

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Ref.1
Chain25 – 15001476Alpha-1-macroglobulin Ref.1
PRO_0000271403
Chain1245 – 1500256Alpha-1-macroglobulin 45 kDa subunit Ref.1
PRO_0000271404

Regions

Region686 – 74661Bait region By similarity UniProtKB P01023
Region1360 – 1500141Receptor-binding domain Ref.4

Amino acid modifications

Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation3821N-linked (GlcNAc...) Potential
Glycosylation4121N-linked (GlcNAc...) Potential
Glycosylation5681N-linked (GlcNAc...) Potential
Glycosylation8831N-linked (GlcNAc...) Potential
Glycosylation9441N-linked (GlcNAc...) Potential
Glycosylation10051N-linked (GlcNAc...) Potential
Glycosylation13901N-linked (GlcNAc...) Potential
Glycosylation14481N-linked (GlcNAc...) Potential
Disulfide bond48 ↔ 86 By similarity UniProtKB P01023
Disulfide bond249 ↔ 298 By similarity UniProtKB P01023
Disulfide bond267 ↔ 286 By similarity UniProtKB P01023
Disulfide bond277Interchain (with C-430) By similarity UniProtKB P01023
Disulfide bond430Interchain (with C-277) By similarity UniProtKB P01023
Disulfide bond469 ↔ 562 By similarity UniProtKB P01023
Disulfide bond594 ↔ 785 By similarity UniProtKB P01023
Disulfide bond642 ↔ 689 By similarity UniProtKB P01023
Disulfide bond835 ↔ 863 By similarity UniProtKB P01023
Disulfide bond861 ↔ 897 By similarity UniProtKB P01023
Disulfide bond935 ↔ 1344 By similarity UniProtKB P01023
Disulfide bond1094 ↔ 1142 By similarity UniProtKB P01023
Cross-link986 ↔ 989Isoglutamyl cysteine thioester (Cys-Gln) By similarity UniProtKB P01023

Experimental info

Sequence conflict501H → A AA sequence Ref.1
Sequence conflict9181I → P AA sequence Ref.1
Sequence conflict924 – 9252IE → AT AA sequence Ref.1

Secondary structure

1 ......................... 1500
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q63041 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8ABB810985795AB2

FASTA1,500167,125
        10         20         30         40         50         60 
MRRNQLPIPV FLLLLLLLPR DATAATGKPR YVVLVPSELY AGVPEKVCVH LNHLNETVTL 

        70         80         90        100        110        120 
NVTLEYGVQY SNLLIDQAVD KDSSYCSSFT ISRPLSPSAL IAVEIKGPTH HFIKKKSMWI 

       130        140        150        160        170        180 
TKAESPVFVQ TDKPIYKPGQ TVKFRVVSVD ISFRPVNETF PVVYIENPKR NRIFQWQNVD 

       190        200        210        220        230        240 
LPGGLHQLSF PLSVEPALGI YKVVVQKDSG KKIEHSFEVK EYVLPKFEVQ VKMPKTMAFL 

       250        260        270        280        290        300 
EEELVVTACG LYTYGKPVPG LVTMKVCRKY TQSYSNCHGQ HSKSICEEFS KQADEKGCFR 

       310        320        330        340        350        360 
QVVKTKVFQP RQKGYDMKIE VEAKIKEDGT GIELTGTGSC EIANTLSKLK FTKANTFYRP 

       370        380        390        400        410        420 
GLPFFGQVLL VDEKGQPIPN KNLTVQVNSV RSQFTFTTDE HGLANILIDT TNFTFSFMGI 

       430        440        450        460        470        480 
RVIYKQNNIC FDNWWVDEYH TQADHSAARI FSPSRSYIQL ELVLGTLACG QTQEIRIHFL 

       490        500        510        520        530        540 
LNEDALKDAK DLTFYYLIKA RGSIFNSGSH VLPLEQGKVK GVVSFPIRVE PGMAPVAKLI 

       550        560        570        580        590        600 
VYTILPNEEL IADVQKFDIE KCFANTVNLS FPSAQSLPAS DTHLTVKATP LSLCALTAVD 

       610        620        630        640        650        660 
QSVLLLKPEA KLSPQSIYNL LPQKAEQGAY LGPLPYKGGE NCIKAEDITH NGIVYTPKQD 

       670        680        690        700        710        720 
LNDNDAYSVF QSIGLKIFTN TRVHKPRYCP MYQAYPPLPY VGEPQALAMS AIPGAGYRSS 

       730        740        750        760        770        780 
NIRTSSMMMM GASEVAQEVE VRETVRKYFP ETWIWDMVPL DLSGDGELPV KVPDTITEWK 

       790        800        810        820        830        840 
ASAFCLSGTT GLGLSSTISH KVFQPFFLEL TLPYSVVRGE AFILKATVLN YMPHCIRIHV 

       850        860        870        880        890        900 
SLEMSPDFLA VPVGSHEDSH CICGNERKTV SWAVTPKSLG EVNFTATAEA LQSPELCGNK 

       910        920        930        940        950        960 
VAEVPALVQK DTVVKPVIVE PEGIEKEQTY NTLLCPQDAE LQENWTLDLP ANVVEGSARA 

       970        980        990       1000       1010       1020 
TQSVLGDILG SAMQNLQNLL QMPYGCGEQN MVLFVPNIYV LEYLNETQQL TEAIKSKAIS 

      1030       1040       1050       1060       1070       1080 
YLISGYQRQL NYQHSDGSYS TFGDRGMRHS QGNTWLTAFV LKAFAQAQSY IYIEKTHITN 

      1090       1100       1110       1120       1130       1140 
AFNWLSMKQR ENGCFQQSGS LLNNAMKGGV DDEVTLSAYI TIALLEMPLP VTHSVVRNAL 

      1150       1160       1170       1180       1190       1200 
FCLETAWASI SNSQESHVYT KALLAYAFAL AGNRAKRSEV LESLNKDAVN EEESVHWQRP 

      1210       1220       1230       1240       1250       1260 
KNVEENVREM RSFSYKPRAP SAEVEMTAYV LLAYLTSASS RPTRDLSSSD LTTASKIVKW 

      1270       1280       1290       1300       1310       1320 
ISKQQNSHGG FSSTQDTVVA LQALSKYGAA TFTKSNKEVS VTIESSGTVS GTLHVNNGNR 

      1330       1340       1350       1360       1370       1380 
LLLQEVRLAD LPGNYITKVS GSGCVYLQTS LKYNILPEAE GEAPFTLKVN TLPLNFDKAE 

      1390       1400       1410       1420       1430       1440 
HHRKFQIHIN VSYIGERPNS NMVIVDVKMV SGFIPVKPSV KKLQDQSNIQ RTEVNTNHVL 

      1450       1460       1470       1480       1490       1500 
IYIEKLTNQT MGFSFAVEQD IPVKNLKPAP VKVYDYYETD EFAIEEYSAP FSSDSEQGNA

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References

[1]"Sequence of rat alpha 1-macroglobulin, a broad-range proteinase inhibitor from the alpha-macroglobulin-complement family."
Eggertsen G., Hudson G., Shiels B., Reed D., Fey G.H.
Mol. Biol. Med. 8:287-302(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-54; 724-743; 901-930 AND 1245-1269, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Fischer 344.
Tissue: Liver.
[2]"cDNA cloning and sequencing of rat alpha 1-macroglobulin."
Warmegard B., Martin N., Johansson S.
Biochemistry 31:2346-2352(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[3]Lubec G., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1287-1294 AND 1321-1327, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[4]"Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer."
Xiao T., DeCamp D.L., Spran S.R.
Protein Sci. 9:1889-1897(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1362-1495.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M77183 mRNA. Translation: AAA40723.1.
M84000 mRNA. Translation: AAA41591.1.
IPIIPI00326140.
PIRA42210.
RefSeqNP_665722.2. NM_145779.2.
UniGeneRn.11295.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EDYX-ray2.30A/B1362-1495[»]
ProteinModelPortalQ63041.
SMRQ63041. Positions 124-225, 1362-1495.
ModBaseSearch...

Protein-protein interaction databases

IntActQ63041. 1 interaction.
STRING10116.ENSRNOP00000009467.

Proteomic databases

PaxDbQ63041.
PRIDEQ63041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009467; ENSRNOP00000009467; ENSRNOG00000006709.
GeneID252922.
KEGGrno:252922.
UCSCRGD:628643. rat.

Organism-specific databases

CTD5858.
RGD628643. Pzp.

Phylogenomic databases

eggNOGCOG2373.
GeneTreeENSGT00560000076685.
HOGENOMHOG000220939.
HOVERGENHBG000039.
InParanoidQ63041.
OMATHITNAF.
OrthoDBEOG4DBTCV.

Gene expression databases

GenevestigatorQ63041.

Family and domain databases

Gene3D2.60.40.690. 1 hit.
InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMSSF49410. AM_receptor_bind. 1 hit.
SSF48239. Terp_cyc_toroid. 1 hit.
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ63041.
NextBio624079.

Entry information

Entry nameA1M_RAT
AccessionPrimary (citable) accession number: Q63041
Secondary accession number(s): Q63332
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents