Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-adducin

Gene

Add1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

GO - Molecular functioni

  • T cell receptor binding Source: RGD

GO - Biological processi

  • cellular response to retinoic acid Source: RGD
  • cytoskeleton organization Source: RGD
  • ion transport Source: RGD
  • negative regulation of actin filament polymerization Source: RGD
  • positive regulation of angiogenesis Source: RGD
  • positive regulation of endocytosis Source: RGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-adducin
Alternative name(s):
Erythrocyte adducin subunit alpha
Gene namesi
Name:Add1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2041. Add1.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • dendrite Source: RGD
  • dendritic spine Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • postsynaptic density Source: RGD
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185321 – 735Alpha-adducinAdd BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei12PhosphoserineCombined sources1
Modified residuei59Phosphoserine; by PKABy similarity1
Modified residuei64PhosphoserineBy similarity1
Modified residuei331PhosphothreonineBy similarity1
Modified residuei334PhosphoserineBy similarity1
Modified residuei353PhosphoserineBy similarity1
Modified residuei355PhosphoserineCombined sources1
Modified residuei358PhosphothreonineCombined sources1
Modified residuei364PhosphoserineBy similarity1
Modified residuei366PhosphoserineCombined sources1
Modified residuei408Phosphoserine; by PKABy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei429PhosphothreonineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei436Phosphoserine; by PKABy similarity1
Modified residuei445Phosphothreonine; by ROCK2By similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei465PhosphoserineCombined sources1
Modified residuei480Phosphothreonine; by ROCK2By similarity1
Modified residuei481Phosphoserine; by PKABy similarity1
Modified residuei586PhosphoserineCombined sources1
Modified residuei600PhosphoserineCombined sources1
Modified residuei605PhosphoserineBy similarity1
Modified residuei610PhosphothreonineCombined sources1
Modified residuei613PhosphoserineBy similarity1
Modified residuei614PhosphothreonineCombined sources1
Modified residuei705PhosphoserineBy similarity1
Modified residuei708PhosphoserineBy similarity1
Modified residuei712PhosphoserineBy similarity1
Modified residuei714Phosphoserine; by PKCBy similarity1
Modified residuei724Phosphoserine; by PKA and PKCBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ63028.
PRIDEiQ63028.

PTM databases

iPTMnetiQ63028.
PhosphoSitePlusiQ63028.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013039.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

GO - Molecular functioni

  • T cell receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018072.

Structurei

3D structure databases

ProteinModelPortaliQ63028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni715 – 732Interaction with calmodulinSequence analysisAdd BLAST18

Domaini

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ63028.
KOiK18622.
PhylomeDBiQ63028.
TreeFamiTF313003.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q63028-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA
110 120 130 140 150
SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDAKCIVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK
360 370 380 390 400
AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLNS
460 470 480 490 500
GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS
610 620 630 640 650
PPDQSAVPNT PPSTPVKLEG GLPQEPTSRD GSDATTSKPT PPDLSPDEPS
660 670 680 690 700
EALAFPTVAE EAHASPDTTQ PLAEADPEPA SASAPGAEEV ASPATEEGSP
710 720 730
MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Length:735
Mass (Da):80,355
Last modified:December 1, 2000 - v2
Checksum:i5DFC5DB452774222
GO
Isoform 2 (identifier: Q63028-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-632: GLPQEPTSRDGS → GDGCAKEYLLP
     633-735: Missing.

Show »
Length:631
Mass (Da):69,749
Checksum:i42AACB92CFC0D4D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti633 – 636DATT → GCHA in CAA88907 (PubMed:8543181).Curated4
Sequence conflicti654A → G in CAA58690 (PubMed:8624703).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti316Y → F in strain: Milan hypersensitive. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000179621 – 632GLPQE…SRDGS → GDGCAKEYLLP in isoform 2. CuratedAdd BLAST12
Alternative sequenceiVSP_000180633 – 735Missing in isoform 2. CuratedAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49081 mRNA. Translation: CAA88906.1.
Z49082 mRNA. Translation: CAA88907.1.
BC107657 mRNA. Translation: AAI07658.1.
X83715 mRNA. Translation: CAA58690.1.
PIRiS54147.
RefSeqiNP_058686.2. NM_016990.2. [Q63028-1]
UniGeneiRn.5788.

Genome annotation databases

GeneIDi24170.
KEGGirno:24170.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49081 mRNA. Translation: CAA88906.1.
Z49082 mRNA. Translation: CAA88907.1.
BC107657 mRNA. Translation: AAI07658.1.
X83715 mRNA. Translation: CAA58690.1.
PIRiS54147.
RefSeqiNP_058686.2. NM_016990.2. [Q63028-1]
UniGeneiRn.5788.

3D structure databases

ProteinModelPortaliQ63028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018072.

PTM databases

iPTMnetiQ63028.
PhosphoSitePlusiQ63028.

Proteomic databases

PaxDbiQ63028.
PRIDEiQ63028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24170.
KEGGirno:24170.

Organism-specific databases

CTDi118.
RGDi2041. Add1.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ63028.
KOiK18622.
PhylomeDBiQ63028.
TreeFamiTF313003.

Miscellaneous databases

PROiQ63028.

Gene expression databases

BgeeiENSRNOG00000013039.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADDA_RAT
AccessioniPrimary (citable) accession number: Q63028
Secondary accession number(s): Q3B7D4, Q64722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.