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Q63016

- LAT1_RAT

UniProt

Q63016 - LAT1_RAT

Protein

Large neutral amino acids transporter small subunit 1

Gene

Slc7a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.2 Publications

    Kineticsi

    1. KM=14.1 µM for L-leucine1 Publication

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: UniProtKB
    2. L-amino acid transmembrane transporter activity Source: Ensembl
    3. peptide antigen binding Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. nervous system development Source: UniProtKB-KW
    3. neutral amino acid transport Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Amino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    SABIO-RKQ63016.

    Protein family/group databases

    TCDBi2.A.3.8.1. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    Integral membrane protein E16
    Short name:
    Protein TA1
    L-type amino acid transporter 1
    Solute carrier family 7 member 5
    Gene namesi
    Name:Slc7a5
    Synonyms:Mpe16, Ta1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi620639. Slc7a5.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Apical cell membrane; Multi-pass membrane protein
    Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Expressed along the retinal capillaries in the ganglion cell and inner plexiform layers. Localized to the apical membrane of placental syncytiophoblastic cells By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. cytosol Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 512512Large neutral amino acids transporter small subunit 1PRO_0000054272Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei46 – 461PhosphothreonineBy similarity
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ63016.
    PRIDEiQ63016.

    Expressioni

    Tissue specificityi

    Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2.5 Publications

    Inductioni

    Expression induced in normal hepatic cells cultured in arginine-depleted medium.1 Publication

    Gene expression databases

    GenevestigatoriQ63016.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.

    Protein-protein interaction databases

    IntActiQ63016. 1 interaction.
    STRINGi10116.ENSRNOP00000025784.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei51 – 7121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei248 – 26821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei324 – 34421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei398 – 41821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei463 – 48321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00730000110280.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ63016.
    KOiK13780.
    OMAiIWLRYRK.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ63016.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q63016-1 [UniParc]FASTAAdd to Basket

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    MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN    50
    ITLINGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG 100
    ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA 150
    LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA 200
    FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG TNLDVGNIVL 250
    ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 300
    YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT 350
    SSRLFFVGSR EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS 400
    IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF 450
    LIAVSFWKTP LECGIGFAII LSGLPVYFFG VWWKNKPKWI LQVIFSVTVL 500
    CQKLMQVVPQ ET 512
    Length:512
    Mass (Da):55,903
    Last modified:January 24, 2001 - v2
    Checksum:iB487CE0B58D73A02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB015432 mRNA. Translation: BAA33035.1.
    U00995 mRNA. Translation: AAA74411.1.
    RefSeqiNP_059049.1. NM_017353.1.
    UniGeneiRn.32261.

    Genome annotation databases

    EnsembliENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824.
    GeneIDi50719.
    KEGGirno:50719.
    UCSCiRGD:620639. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB015432 mRNA. Translation: BAA33035.1 .
    U00995 mRNA. Translation: AAA74411.1 .
    RefSeqi NP_059049.1. NM_017353.1.
    UniGenei Rn.32261.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q63016. 1 interaction.
    STRINGi 10116.ENSRNOP00000025784.

    Chemistry

    BindingDBi Q63016.
    ChEMBLi CHEMBL4149.

    Protein family/group databases

    TCDBi 2.A.3.8.1. the amino acid-polyamine-organocation (apc) family.

    Proteomic databases

    PaxDbi Q63016.
    PRIDEi Q63016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025784 ; ENSRNOP00000025784 ; ENSRNOG00000018824 .
    GeneIDi 50719.
    KEGGi rno:50719.
    UCSCi RGD:620639. rat.

    Organism-specific databases

    CTDi 8140.
    RGDi 620639. Slc7a5.

    Phylogenomic databases

    eggNOGi COG0531.
    GeneTreei ENSGT00730000110280.
    HOGENOMi HOG000098892.
    HOVERGENi HBG000476.
    InParanoidi Q63016.
    KOi K13780.
    OMAi IWLRYRK.
    OrthoDBi EOG73BVCR.
    PhylomeDBi Q63016.
    TreeFami TF313355.

    Enzyme and pathway databases

    SABIO-RK Q63016.

    Miscellaneous databases

    NextBioi 610578.
    PROi Q63016.

    Gene expression databases

    Genevestigatori Q63016.

    Family and domain databases

    InterProi IPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view ]
    PANTHERi PTHR11785. PTHR11785. 1 hit.
    Pfami PF13520. AA_permease_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsi TIGR00911. 2A0308. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)."
      Kanai Y., Segawa H., Miyamoto K., Uchino H., Takeda E., Endou H.
      J. Biol. Chem. 273:23629-23632(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation."
      Sang J., Lim Y.P., Panzica M., Finch P., Thompson N.L.
      Cancer Res. 55:1152-1159(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-512, TISSUE SPECIFICITY.
      Tissue: Hepatoma.
    3. "Expression of a system L neutral amino acid transporter at the blood-brain barrier."
      Matsuo H., Tsukada S., Nakata T., Chairoungdua A., Kim D.K., Cha S.H., Inatomi J., Yorifuji H., Fukuda J., Endou H., Kanai Y.
      NeuroReport 11:3507-3511(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    5. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
      Fraga S., Pinho M.J., Soares-da-Silva P.
      Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
      Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
      Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.

    Entry informationi

    Entry nameiLAT1_RAT
    AccessioniPrimary (citable) accession number: Q63016
    Secondary accession number(s): Q9QWL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3