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Protein

Large neutral amino acids transporter small subunit 1

Gene

Slc7a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (By similarity).By similarity2 Publications

Miscellaneous

L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).

Kineticsi

  1. KM=14.1 µM for L-leucine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionDevelopmental protein
    Biological processAmino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    ReactomeiR-RNO-210991 Basigin interactions
    R-RNO-352230 Amino acid transport across the plasma membrane
    R-RNO-71240 Tryptophan catabolism
    SABIO-RKQ63016

    Protein family/group databases

    TCDBi2.A.3.8.1 the amino acid-polyamine-organocation (apc) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    Integral membrane protein E16
    Short name:
    Protein TA1
    L-type amino acid transporter 1
    Solute carrier family 7 member 5
    Gene namesi
    Name:Slc7a5
    Synonyms:Mpe16, Ta1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 19

    Organism-specific databases

    RGDi620639 Slc7a5

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
    Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
    Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
    Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
    Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
    Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
    Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
    Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
    Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
    Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
    Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
    Transmembranei463 – 483HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL4149

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000542721 – 512Large neutral amino acids transporter small subunit 1Add BLAST512

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei46PhosphothreonineBy similarity1
    Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ63016
    PRIDEiQ63016

    Expressioni

    Tissue specificityi

    Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2.5 Publications

    Inductioni

    Expression induced in normal hepatic cells cultured in arginine-depleted medium.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000018824
    GenevisibleiQ63016 RN

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Interacts with LAPTM4B; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation.By similarity

    Protein-protein interaction databases

    BioGridi248432, 1 interactor
    IntActiQ63016, 1 interactor
    STRINGi10116.ENSRNOP00000025784

    Chemistry databases

    BindingDBiQ63016

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1287 Eukaryota
    COG0531 LUCA
    GeneTreeiENSGT00760000119037
    HOGENOMiHOG000098892
    HOVERGENiHBG000476
    InParanoidiQ63016
    KOiK13780
    OMAiHKAFGIH
    OrthoDBiEOG091G07EM
    PhylomeDBiQ63016
    TreeFamiTF313355

    Family and domain databases

    InterProiView protein in InterPro
    IPR002293 AA/rel_permease1
    IPR004760 L_AA_transporter
    PfamiView protein in Pfam
    PF13520 AA_permease_2, 1 hit
    PIRSFiPIRSF006060 AA_transporter, 1 hit
    TIGRFAMsiTIGR00911 2A0308, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q63016-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN
    60 70 80 90 100
    ITLINGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG
    110 120 130 140 150
    ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA
    160 170 180 190 200
    LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA
    210 220 230 240 250
    FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG TNLDVGNIVL
    260 270 280 290 300
    ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA
    310 320 330 340 350
    YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT
    360 370 380 390 400
    SSRLFFVGSR EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS
    410 420 430 440 450
    IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF
    460 470 480 490 500
    LIAVSFWKTP LECGIGFAII LSGLPVYFFG VWWKNKPKWI LQVIFSVTVL
    510
    CQKLMQVVPQ ET
    Length:512
    Mass (Da):55,903
    Last modified:January 24, 2001 - v2
    Checksum:iB487CE0B58D73A02
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB015432 mRNA Translation: BAA33035.1
    U00995 mRNA Translation: AAA74411.1
    RefSeqiNP_059049.1, NM_017353.1
    UniGeneiRn.32261

    Genome annotation databases

    EnsembliENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824
    GeneIDi50719
    KEGGirno:50719
    UCSCiRGD:620639 rat

    Similar proteinsi

    Entry informationi

    Entry nameiLAT1_RAT
    AccessioniPrimary (citable) accession number: Q63016
    Secondary accession number(s): Q9QWL4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 24, 2001
    Last modified: May 23, 2018
    This is version 135 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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