Large neutral amino acids transporter small subunit 1

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Q63016 (LAT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Large neutral amino acids transporter small subunit 1

Alternative name(s):
4F2 light chain
Short name=4F2 LC
Short name=4F2LC
Integral membrane protein E16
Short name=Protein TA1
L-type amino acid transporter 1
Solute carrier family 7 member 5

Gene names

Name:	Slc7a5
Synonyms:	Mpe16, Ta1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Ref.4 Ref.6
Subunit structure	Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.
Subcellular location	Cytoplasm › cytosol By similarity. Apical cell membrane; Multi-pass membrane protein. Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Expressed along the retinal capillaries in the ganglion cell and inner plexiform layers. Localized to the apical membrane of placental syncytiophoblastic cells By similarity. Ref.3 Ref.5 Ref.6
Tissue specificity	Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6
Induction	Expression induced in normal hepatic cells cultured in arginine-depleted medium. Ref.4
Miscellaneous	L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=14.1 µM for L-leucine Ref.6

Ontologies

Keywords
Biological process	Amino-acid transport Differentiation Neurogenesis Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW neutral amino acid transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-amino acid transmembrane transporter activity Inferred from electronic annotation. Source: Compara amino acid transmembrane transporter activity Inferred from sequence or structural similarity. Source: UniProtKB peptide antigen binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 512

512

Large neutral amino acids transporter small subunit 1

PRO_0000054272

Regions

Transmembrane

51 – 71

Helical; Potential

Transmembrane

85 – 105

Helical; Potential

Transmembrane

121 – 141

Helical; Potential

Transmembrane

147 – 167

Helical; Potential

Transmembrane

174 – 194

Helical; Potential

Transmembrane

200 – 220

Helical; Potential

Transmembrane

248 – 268

Helical; Potential

Transmembrane

279 – 299

Helical; Potential

Transmembrane

324 – 344

Helical; Potential

Transmembrane

398 – 418

Helical; Potential

Transmembrane

436 – 456

Helical; Potential

Transmembrane

463 – 483

Helical; Potential

Amino acid modifications

Modified residue

512

Phosphothreonine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

345

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q63016 [UniParc].

Last modified January 24, 2001. Version 2.
Checksum: B487CE0B58D73A02
Show »

FASTA

512

55,903

        10         20         30         40         50         60 
MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLINGVAII 

        70         80         90        100        110        120 
VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 

       130        140        150        160        170        180 
MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 

       190        200        210        220        230        240 
LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG 

       250        260        270        280        290        300 
TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 

       310        320        330        340        350        360 
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 

       370        380        390        400        410        420 
EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 

       430        440        450        460        470        480 
MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP LECGIGFAII LSGLPVYFFG 

       490        500        510 
VWWKNKPKWI LQVIFSVTVL CQKLMQVVPQ ET

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References

[1]	"Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)." Kanai Y., Segawa H., Miyamoto K., Uchino H., Takeda E., Endou H. J. Biol. Chem. 273:23629-23632(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation." Sang J., Lim Y.P., Panzica M., Finch P., Thompson N.L. Cancer Res. 55:1152-1159(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-512, TISSUE SPECIFICITY. Tissue: Hepatoma.
[3]	"Expression of a system L neutral amino acid transporter at the blood-brain barrier." Matsuo H., Tsukada S., Nakata T., Chairoungdua A., Kim D.K., Cha S.H., Inatomi J., Yorifuji H., Fukuda J., Endou H., Kanai Y. NeuroReport 11:3507-3511(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"Transcriptional regulation of the LAT-1/CD98 light chain." Padbury J.F., Diah S.K., McGonnigal B., Miller C., Fugere C., Kuzniar M., Thompson N.L. Biochem. Biophys. Res. Commun. 318:529-534(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[5]	"Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells." Fraga S., Pinho M.J., Soares-da-Silva P. Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier." Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K. Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015432 mRNA. Translation: BAA33035.1. U00995 mRNA. Translation: AAA74411.1.
IPI	IPI00204778.
RefSeq	NP_059049.1. NM_017353.1.
UniGene	Rn.32261.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q63016. 1 interaction.
STRING	10116.ENSRNOP00000025784.
Protein family/group databases
TCDB	2.A.3.8.1. amino acid-polyamine-organocation (APC) family.
Proteomic databases
PaxDb	Q63016.
PRIDE	Q63016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824.
GeneID	50719.
KEGG	rno:50719.
UCSC	RGD:620639. rat.
Organism-specific databases
CTD	8140.
RGD	620639. Slc7a5.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00690000101731.
HOGENOM	HOG000098892.
HOVERGEN	HBG000476.
InParanoid	Q63016.
KO	K13780.
OMA	PIVTAVY.
OrthoDB	EOG4J1186.
Gene expression databases
Genevestigator	Q63016.
GermOnline	ENSRNOG00000018824. Rattus norvegicus.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004760. L_AA_transporter. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00911. 2A0308. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q63016.
ChEMBL	CHEMBL4149.
NextBio	610578.

Entry information

Entry name

LAT1_RAT

Accession

Primary (citable) accession number: Q63016
Secondary accession number(s): Q9QWL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 24, 2001
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents