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Q63016 (LAT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large neutral amino acids transporter small subunit 1
Alternative name(s):
4F2 light chain
Short name=4F2 LC
Short name=4F2LC
Integral membrane protein E16
Short name=Protein TA1
L-type amino acid transporter 1
Solute carrier family 7 member 5
Gene names
Name:Slc7a5
Synonyms:Mpe16, Ta1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Ref.4 Ref.6

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.

Subcellular location

Cytoplasmcytosol By similarity. Apical cell membrane; Multi-pass membrane protein. Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Expressed along the retinal capillaries in the ganglion cell and inner plexiform layers. Localized to the apical membrane of placental syncytiophoblastic cells By similarity. Ref.3 Ref.5 Ref.6

Tissue specificity

Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6

Induction

Expression induced in normal hepatic cells cultured in arginine-depleted medium. Ref.4

Miscellaneous

L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=14.1 µM for L-leucine Ref.6

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Large neutral amino acids transporter small subunit 1
PRO_0000054272

Regions

Transmembrane51 – 7121Helical; Potential
Transmembrane85 – 10521Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane248 – 26821Helical; Potential
Transmembrane279 – 29921Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane398 – 41821Helical; Potential
Transmembrane436 – 45621Helical; Potential
Transmembrane463 – 48321Helical; Potential

Amino acid modifications

Modified residue461Phosphothreonine By similarity
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q63016 [UniParc].

Last modified January 24, 2001. Version 2.
Checksum: B487CE0B58D73A02

FASTA51255,903
        10         20         30         40         50         60 
MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLINGVAII 

        70         80         90        100        110        120 
VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 

       130        140        150        160        170        180 
MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 

       190        200        210        220        230        240 
LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG 

       250        260        270        280        290        300 
TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 

       310        320        330        340        350        360 
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 

       370        380        390        400        410        420 
EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 

       430        440        450        460        470        480 
MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP LECGIGFAII LSGLPVYFFG 

       490        500        510 
VWWKNKPKWI LQVIFSVTVL CQKLMQVVPQ ET 

« Hide

References

[1]"Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)."
Kanai Y., Segawa H., Miyamoto K., Uchino H., Takeda E., Endou H.
J. Biol. Chem. 273:23629-23632(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation."
Sang J., Lim Y.P., Panzica M., Finch P., Thompson N.L.
Cancer Res. 55:1152-1159(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-512, TISSUE SPECIFICITY.
Tissue: Hepatoma.
[3]"Expression of a system L neutral amino acid transporter at the blood-brain barrier."
Matsuo H., Tsukada S., Nakata T., Chairoungdua A., Kim D.K., Cha S.H., Inatomi J., Yorifuji H., Fukuda J., Endou H., Kanai Y.
NeuroReport 11:3507-3511(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"Transcriptional regulation of the LAT-1/CD98 light chain."
Padbury J.F., Diah S.K., McGonnigal B., Miller C., Fugere C., Kuzniar M., Thompson N.L.
Biochem. Biophys. Res. Commun. 318:529-534(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[5]"Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
Fraga S., Pinho M.J., Soares-da-Silva P.
Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015432 mRNA. Translation: BAA33035.1.
U00995 mRNA. Translation: AAA74411.1.
RefSeqNP_059049.1. NM_017353.1.
UniGeneRn.32261.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ63016. 1 interaction.
STRING10116.ENSRNOP00000025784.

Chemistry

BindingDBQ63016.
ChEMBLCHEMBL4149.

Protein family/group databases

TCDB2.A.3.8.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbQ63016.
PRIDEQ63016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824.
GeneID50719.
KEGGrno:50719.
UCSCRGD:620639. rat.

Organism-specific databases

CTD8140.
RGD620639. Slc7a5.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00730000110280.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ63016.
KOK13780.
OMAIWLRYRK.
OrthoDBEOG73BVCR.
PhylomeDBQ63016.
TreeFamTF313355.

Enzyme and pathway databases

SABIO-RKQ63016.

Gene expression databases

GenevestigatorQ63016.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00911. 2A0308. 1 hit.
ProtoNetSearch...

Other

NextBio610578.
PROQ63016.

Entry information

Entry nameLAT1_RAT
AccessionPrimary (citable) accession number: Q63016
Secondary accession number(s): Q9QWL4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 24, 2001
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families