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Q63016

- LAT1_RAT

UniProt

Q63016 - LAT1_RAT

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Protein
Large neutral amino acids transporter small subunit 1
Gene
Slc7a5, Mpe16, Ta1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.2 Publications

Kineticsi

  1. KM=14.1 µM for L-leucine1 Publication

GO - Molecular functioni

  1. L-amino acid transmembrane transporter activity Source: Ensembl
  2. amino acid transmembrane transporter activity Source: UniProtKB
  3. peptide antigen binding Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. nervous system development Source: UniProtKB-KW
  3. neutral amino acid transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Amino-acid transport, Differentiation, Neurogenesis, Transport

Enzyme and pathway databases

SABIO-RKQ63016.

Protein family/group databases

TCDBi2.A.3.8.1. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Large neutral amino acids transporter small subunit 1
Alternative name(s):
4F2 light chain
Short name:
4F2 LC
Short name:
4F2LC
Integral membrane protein E16
Short name:
Protein TA1
L-type amino acid transporter 1
Solute carrier family 7 member 5
Gene namesi
Name:Slc7a5
Synonyms:Mpe16, Ta1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi620639. Slc7a5.

Subcellular locationi

Cytoplasmcytosol By similarity. Apical cell membrane; Multi-pass membrane protein
Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells. Expressed along the retinal capillaries in the ganglion cell and inner plexiform layers. Localized to the apical membrane of placental syncytiophoblastic cells By similarity.3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei51 – 7121Helical; Reviewed prediction
Add
BLAST
Transmembranei85 – 10521Helical; Reviewed prediction
Add
BLAST
Transmembranei121 – 14121Helical; Reviewed prediction
Add
BLAST
Transmembranei147 – 16721Helical; Reviewed prediction
Add
BLAST
Transmembranei174 – 19421Helical; Reviewed prediction
Add
BLAST
Transmembranei200 – 22021Helical; Reviewed prediction
Add
BLAST
Transmembranei248 – 26821Helical; Reviewed prediction
Add
BLAST
Transmembranei279 – 29921Helical; Reviewed prediction
Add
BLAST
Transmembranei324 – 34421Helical; Reviewed prediction
Add
BLAST
Transmembranei398 – 41821Helical; Reviewed prediction
Add
BLAST
Transmembranei436 – 45621Helical; Reviewed prediction
Add
BLAST
Transmembranei463 – 48321Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. cytosol Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Large neutral amino acids transporter small subunit 1
PRO_0000054272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461Phosphothreonine By similarity
Glycosylationi50 – 501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi345 – 3451N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63016.
PRIDEiQ63016.

Expressioni

Tissue specificityi

Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2.5 Publications

Inductioni

Expression induced in normal hepatic cells cultured in arginine-depleted medium.1 Publication

Gene expression databases

GenevestigatoriQ63016.

Interactioni

Subunit structurei

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.

Protein-protein interaction databases

IntActiQ63016. 1 interaction.
STRINGi10116.ENSRNOP00000025784.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
GeneTreeiENSGT00730000110280.
HOGENOMiHOG000098892.
HOVERGENiHBG000476.
InParanoidiQ63016.
KOiK13780.
OMAiIWLRYRK.
OrthoDBiEOG73BVCR.
PhylomeDBiQ63016.
TreeFamiTF313355.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00911. 2A0308. 1 hit.

Sequencei

Sequence statusi: Complete.

Q63016-1 [UniParc]FASTAAdd to Basket

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MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN    50
ITLINGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG 100
ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA 150
LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA 200
FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG TNLDVGNIVL 250
ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 300
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT 350
SSRLFFVGSR EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS 400
IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF 450
LIAVSFWKTP LECGIGFAII LSGLPVYFFG VWWKNKPKWI LQVIFSVTVL 500
CQKLMQVVPQ ET 512
Length:512
Mass (Da):55,903
Last modified:January 24, 2001 - v2
Checksum:iB487CE0B58D73A02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015432 mRNA. Translation: BAA33035.1.
U00995 mRNA. Translation: AAA74411.1.
RefSeqiNP_059049.1. NM_017353.1.
UniGeneiRn.32261.

Genome annotation databases

EnsembliENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824.
GeneIDi50719.
KEGGirno:50719.
UCSCiRGD:620639. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015432 mRNA. Translation: BAA33035.1 .
U00995 mRNA. Translation: AAA74411.1 .
RefSeqi NP_059049.1. NM_017353.1.
UniGenei Rn.32261.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q63016. 1 interaction.
STRINGi 10116.ENSRNOP00000025784.

Chemistry

BindingDBi Q63016.
ChEMBLi CHEMBL4149.

Protein family/group databases

TCDBi 2.A.3.8.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbi Q63016.
PRIDEi Q63016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025784 ; ENSRNOP00000025784 ; ENSRNOG00000018824 .
GeneIDi 50719.
KEGGi rno:50719.
UCSCi RGD:620639. rat.

Organism-specific databases

CTDi 8140.
RGDi 620639. Slc7a5.

Phylogenomic databases

eggNOGi COG0531.
GeneTreei ENSGT00730000110280.
HOGENOMi HOG000098892.
HOVERGENi HBG000476.
InParanoidi Q63016.
KOi K13780.
OMAi IWLRYRK.
OrthoDBi EOG73BVCR.
PhylomeDBi Q63016.
TreeFami TF313355.

Enzyme and pathway databases

SABIO-RK Q63016.

Miscellaneous databases

NextBioi 610578.
PROi Q63016.

Gene expression databases

Genevestigatori Q63016.

Family and domain databases

InterProi IPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF13520. AA_permease_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006060. AA_transporter. 1 hit.
TIGRFAMsi TIGR00911. 2A0308. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)."
    Kanai Y., Segawa H., Miyamoto K., Uchino H., Takeda E., Endou H.
    J. Biol. Chem. 273:23629-23632(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "TA1, a highly conserved oncofetal complementary DNA from rat hepatoma, encodes an integral membrane protein associated with liver development, carcinogenesis, and cell activation."
    Sang J., Lim Y.P., Panzica M., Finch P., Thompson N.L.
    Cancer Res. 55:1152-1159(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 272-512, TISSUE SPECIFICITY.
    Tissue: Hepatoma.
  3. "Expression of a system L neutral amino acid transporter at the blood-brain barrier."
    Matsuo H., Tsukada S., Nakata T., Chairoungdua A., Kim D.K., Cha S.H., Inatomi J., Yorifuji H., Fukuda J., Endou H., Kanai Y.
    NeuroReport 11:3507-3511(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  5. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
    Fraga S., Pinho M.J., Soares-da-Silva P.
    Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
    Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
    Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.

Entry informationi

Entry nameiLAT1_RAT
AccessioniPrimary (citable) accession number: Q63016
Secondary accession number(s): Q9QWL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 24, 2001
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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