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Q63010

- EST5_RAT

UniProt

Q63010 - EST5_RAT

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Protein

Liver carboxylesterase B-1

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei466 – 4661Charge relay systemBy similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

MEROPSiS09.969.

Names & Taxonomyi

Protein namesi
Recommended name:
Liver carboxylesterase B-1 (EC:3.1.1.1)
Alternative name(s):
Liver microsomal carboxylesterase
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Subcellular locationi

Endoplasmic reticulum lumen
Note: Microsomal membrane, lumen of endoplasmic reticulum.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 561543Liver carboxylesterase B-1PRO_0000008582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi87 ↔ 116By similarity
Disulfide bondi273 ↔ 284By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ63010.

PTM databases

PhosphoSiteiQ63010.

Expressioni

Gene expression databases

GenevestigatoriQ63010.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

MINTiMINT-4997337.
STRINGi10116.ENSRNOP00000024187.

Structurei

3D structure databases

ProteinModelPortaliQ63010.
SMRiQ63010. Positions 21-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi558 – 5614Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008839.
PhylomeDBiQ63010.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63010-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCLRSLFLVS LATCVVCGNP SSPPVVDTMK GKVLGKYASL EGVTQSVAVF
60 70 80 90 100
LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD ATKGQRMNDL
110 120 130 140 150
LTNRKEKVHL QFSEDCLYLN IYTPADFTKD SRMPVMVWIH GGGLTQGGAS
160 170 180 190 200
TYDGQVLSAY ENVVVVAIQY RLGIWGFFST GDEHSRGNWG HLDQVAALHW
210 220 230 240 250
VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLSKNLY HRAISESGVV
260 270 280 290 300
LITELFTKDV RPAAKQIADM AGCKTTTSAI IVHCLRQKTE EELLEIMEKM
310 320 330 340 350
NLIKLSSQRD TKESYHFLST VIDDVVLPKD PKEILAEKNF NTVPYIVGIN
360 370 380 390 400
KQECGWLLPT MMRFVPPDVK LDKKMAIMLL EKFASIYGIP EDIIPVAIEK
410 420 430 440 450
YRKGSDDPIK IRDGILAFIG DVLFCIPSVM VSRDHRDAGA PTYVYEYQYY
460 470 480 490 500
PSFSSPQRPK DVVGDHADDV YSVFGAPILR DGASEEEIKL SKMVMKFWAN
510 520 530 540 550
FARNGNPNAR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE EVAFWTQLLA
560
KRQPQPHHNE L
Length:561
Mass (Da):62,495
Last modified:November 1, 1996 - v1
Checksum:i013C1C13ACEBC6C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191N → D AA sequence (PubMed:8597091)Curated
Sequence conflicti29 – 291M → V AA sequence (PubMed:8597091)Curated
Sequence conflicti38 – 381A → V AA sequence (PubMed:8597091)Curated
Sequence conflicti422 – 4221V → A in AAH79129. (PubMed:15489334)Curated
Sequence conflicti509 – 5091A → G in AAH79129. (PubMed:15489334)Curated
Sequence conflicti536 – 5361R → G in AAH79129. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10698 mRNA. Translation: AAA64639.1.
BC079129 mRNA. Translation: AAH79129.1.
PIRiS71597.
RefSeqiNP_001019536.1. NM_001024365.1.
UniGeneiRn.202952.

Genome annotation databases

GeneIDi501232.
KEGGirno:501232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10698 mRNA. Translation: AAA64639.1 .
BC079129 mRNA. Translation: AAH79129.1 .
PIRi S71597.
RefSeqi NP_001019536.1. NM_001024365.1.
UniGenei Rn.202952.

3D structure databases

ProteinModelPortali Q63010.
SMRi Q63010. Positions 21-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4997337.
STRINGi 10116.ENSRNOP00000024187.

Chemistry

ChEMBLi CHEMBL2366498.

Protein family/group databases

MEROPSi S09.969.

PTM databases

PhosphoSitei Q63010.

Proteomic databases

PRIDEi Q63010.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 501232.
KEGGi rno:501232.

Organism-specific databases

CTDi 501232.

Phylogenomic databases

HOVERGENi HBG008839.
PhylomeDBi Q63010.

Miscellaneous databases

NextBioi 708661.

Gene expression databases

Genevestigatori Q63010.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view ]
Pfami PF00135. COesterase. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
    Yan B., Yang D., Brady M., Parkinson A.
    J. Biol. Chem. 269:29688-29696(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Kidney.
  3. "Molecular aspects of carboxylesterase isoforms in comparison with other esterases."
    Satoh T., Hosokawa M.
    Toxicol. Lett. 82:439-445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-40, NUCLEOTIDE SEQUENCE [MRNA] OF 113-121; 138-149; 216-224; 350-356 AND 464-469, SUBUNIT.
    Tissue: Liver.

Entry informationi

Entry nameiEST5_RAT
AccessioniPrimary (citable) accession number: Q63010
Secondary accession number(s): Q6AYA3, Q9QUX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3