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Q63010

- EST5_RAT

UniProt

Q63010 - EST5_RAT

Protein

Liver carboxylesterase B-1

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.

    Catalytic activityi

    A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei353 – 3531Charge relay systemBy similarity
    Active sitei466 – 4661Charge relay systemBy similarity

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Protein family/group databases

    MEROPSiS09.969.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Liver carboxylesterase B-1 (EC:3.1.1.1)
    Alternative name(s):
    Liver microsomal carboxylesterase
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Subcellular locationi

    Endoplasmic reticulum lumen
    Note: Microsomal membrane, lumen of endoplasmic reticulum.

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 561543Liver carboxylesterase B-1PRO_0000008582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi87 ↔ 116By similarity
    Disulfide bondi273 ↔ 284By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ63010.

    PTM databases

    PhosphoSiteiQ63010.

    Expressioni

    Gene expression databases

    GenevestigatoriQ63010.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    MINTiMINT-4997337.
    STRINGi10116.ENSRNOP00000024187.

    Structurei

    3D structure databases

    ProteinModelPortaliQ63010.
    SMRiQ63010. Positions 21-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi558 – 5614Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG008839.
    PhylomeDBiQ63010.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q63010-1 [UniParc]FASTAAdd to Basket

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    MCLRSLFLVS LATCVVCGNP SSPPVVDTMK GKVLGKYASL EGVTQSVAVF    50
    LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD ATKGQRMNDL 100
    LTNRKEKVHL QFSEDCLYLN IYTPADFTKD SRMPVMVWIH GGGLTQGGAS 150
    TYDGQVLSAY ENVVVVAIQY RLGIWGFFST GDEHSRGNWG HLDQVAALHW 200
    VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLSKNLY HRAISESGVV 250
    LITELFTKDV RPAAKQIADM AGCKTTTSAI IVHCLRQKTE EELLEIMEKM 300
    NLIKLSSQRD TKESYHFLST VIDDVVLPKD PKEILAEKNF NTVPYIVGIN 350
    KQECGWLLPT MMRFVPPDVK LDKKMAIMLL EKFASIYGIP EDIIPVAIEK 400
    YRKGSDDPIK IRDGILAFIG DVLFCIPSVM VSRDHRDAGA PTYVYEYQYY 450
    PSFSSPQRPK DVVGDHADDV YSVFGAPILR DGASEEEIKL SKMVMKFWAN 500
    FARNGNPNAR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE EVAFWTQLLA 550
    KRQPQPHHNE L 561
    Length:561
    Mass (Da):62,495
    Last modified:November 1, 1996 - v1
    Checksum:i013C1C13ACEBC6C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191N → D AA sequence (PubMed:8597091)Curated
    Sequence conflicti29 – 291M → V AA sequence (PubMed:8597091)Curated
    Sequence conflicti38 – 381A → V AA sequence (PubMed:8597091)Curated
    Sequence conflicti422 – 4221V → A in AAH79129. (PubMed:15489334)Curated
    Sequence conflicti509 – 5091A → G in AAH79129. (PubMed:15489334)Curated
    Sequence conflicti536 – 5361R → G in AAH79129. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10698 mRNA. Translation: AAA64639.1.
    BC079129 mRNA. Translation: AAH79129.1.
    PIRiS71597.
    RefSeqiNP_001019536.1. NM_001024365.1.
    UniGeneiRn.202952.

    Genome annotation databases

    GeneIDi501232.
    KEGGirno:501232.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10698 mRNA. Translation: AAA64639.1 .
    BC079129 mRNA. Translation: AAH79129.1 .
    PIRi S71597.
    RefSeqi NP_001019536.1. NM_001024365.1.
    UniGenei Rn.202952.

    3D structure databases

    ProteinModelPortali Q63010.
    SMRi Q63010. Positions 21-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997337.
    STRINGi 10116.ENSRNOP00000024187.

    Protein family/group databases

    MEROPSi S09.969.

    PTM databases

    PhosphoSitei Q63010.

    Proteomic databases

    PRIDEi Q63010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 501232.
    KEGGi rno:501232.

    Organism-specific databases

    CTDi 501232.

    Phylogenomic databases

    HOVERGENi HBG008839.
    PhylomeDBi Q63010.

    Miscellaneous databases

    NextBioi 708661.

    Gene expression databases

    Genevestigatori Q63010.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
      Yan B., Yang D., Brady M., Parkinson A.
      J. Biol. Chem. 269:29688-29696(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Brown Norway.
      Tissue: Kidney.
    3. "Molecular aspects of carboxylesterase isoforms in comparison with other esterases."
      Satoh T., Hosokawa M.
      Toxicol. Lett. 82:439-445(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-40, NUCLEOTIDE SEQUENCE [MRNA] OF 113-121; 138-149; 216-224; 350-356 AND 464-469, SUBUNIT.
      Tissue: Liver.

    Entry informationi

    Entry nameiEST5_RAT
    AccessioniPrimary (citable) accession number: Q63010
    Secondary accession number(s): Q6AYA3, Q9QUX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3