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Q63010 (EST5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Liver carboxylesterase B-1

EC=3.1.1.1
Alternative name(s):
Liver microsomal carboxylesterase
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.

Catalytic activity

A carboxylic ester + H2O = an alcohol + a carboxylate.

Subunit structure

Monomer. Ref.3

Subcellular location

Endoplasmic reticulum lumen. Note: Microsomal membrane, lumen of endoplasmic reticulum.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
   DomainSignal
   Molecular functionHydrolase
Serine esterase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarboxylic ester hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Ref.3
Chain19 – 561543Liver carboxylesterase B-1
PRO_0000008582

Regions

Motif558 – 5614Prevents secretion from ER Potential

Sites

Active site2211Acyl-ester intermediate By similarity
Active site3531Charge relay system By similarity
Active site4661Charge relay system By similarity

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond87 ↔ 116 By similarity
Disulfide bond273 ↔ 284 By similarity

Experimental info

Sequence conflict191N → D AA sequence Ref.3
Sequence conflict291M → V AA sequence Ref.3
Sequence conflict381A → V AA sequence Ref.3
Sequence conflict4221V → A in AAH79129. Ref.2
Sequence conflict5091A → G in AAH79129. Ref.2
Sequence conflict5361R → G in AAH79129. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q63010 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 013C1C13ACEBC6C9

FASTA56162,495
        10         20         30         40         50         60 
MCLRSLFLVS LATCVVCGNP SSPPVVDTMK GKVLGKYASL EGVTQSVAVF LGVPFAKPPL 

        70         80         90        100        110        120 
GSLRFAPPQP AEPWSFVKNT TTYPPMCSQD ATKGQRMNDL LTNRKEKVHL QFSEDCLYLN 

       130        140        150        160        170        180 
IYTPADFTKD SRMPVMVWIH GGGLTQGGAS TYDGQVLSAY ENVVVVAIQY RLGIWGFFST 

       190        200        210        220        230        240 
GDEHSRGNWG HLDQVAALHW VQDNIANFGG DPGSVTIFGE SAGGFSVSVL VLSPLSKNLY 

       250        260        270        280        290        300 
HRAISESGVV LITELFTKDV RPAAKQIADM AGCKTTTSAI IVHCLRQKTE EELLEIMEKM 

       310        320        330        340        350        360 
NLIKLSSQRD TKESYHFLST VIDDVVLPKD PKEILAEKNF NTVPYIVGIN KQECGWLLPT 

       370        380        390        400        410        420 
MMRFVPPDVK LDKKMAIMLL EKFASIYGIP EDIIPVAIEK YRKGSDDPIK IRDGILAFIG 

       430        440        450        460        470        480 
DVLFCIPSVM VSRDHRDAGA PTYVYEYQYY PSFSSPQRPK DVVGDHADDV YSVFGAPILR 

       490        500        510        520        530        540 
DGASEEEIKL SKMVMKFWAN FARNGNPNAR GLPHWPQYDQ KEEYLQIGAT TQQSQRLKAE 

       550        560 
EVAFWTQLLA KRQPQPHHNE L 

« Hide

References

« Hide 'large scale' references
[1]"Rat kidney carboxylesterase. Cloning, sequencing, cellular localization, and relationship to rat liver hydrolase."
Yan B., Yang D., Brady M., Parkinson A.
J. Biol. Chem. 269:29688-29696(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
[3]"Molecular aspects of carboxylesterase isoforms in comparison with other esterases."
Satoh T., Hosokawa M.
Toxicol. Lett. 82:439-445(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 19-40, NUCLEOTIDE SEQUENCE [MRNA] OF 113-121; 138-149; 216-224; 350-356 AND 464-469, SUBUNIT.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10698 mRNA. Translation: AAA64639.1.
BC079129 mRNA. Translation: AAH79129.1.
PIRS71597.
RefSeqNP_001019536.1. NM_001024365.1.
UniGeneRn.202952.

3D structure databases

ProteinModelPortalQ63010.
SMRQ63010. Positions 21-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997337.
STRING10116.ENSRNOP00000024187.

Protein family/group databases

MEROPSS09.969.

PTM databases

PhosphoSiteQ63010.

Proteomic databases

PRIDEQ63010.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID501232.
KEGGrno:501232.

Organism-specific databases

CTD501232.

Phylogenomic databases

HOVERGENHBG008839.
PhylomeDBQ63010.

Gene expression databases

GenevestigatorQ63010.

Family and domain databases

InterProIPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio708661.

Entry information

Entry nameEST5_RAT
AccessionPrimary (citable) accession number: Q63010
Secondary accession number(s): Q6AYA3, Q9QUX6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families