Reviewed, UniProtKB/Swiss-Prot Q62MP8 (QUEF_BURMA)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	BMA0180

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163024

Sequences

	Sequence		Length	Mass (Da)
	Q62MP8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 53ECAF5D759A9272	FASTA	274	30,401
	10 20 30 40 50 60 MNPEHSPLGK ATVYANQYDA SLLFPIPRAG AREQIGIGAP LPFFGTDIWN AYELSWLNAR 70 80 90 100 110 120 GKPQIAIATF YVPAESPNIV ESKSFKLYLG SFAQTAFESA DAVRDALKRD VSAACGASVT 130 140 150 160 170 180 VRLATPAEFR KLQMDELDGL SLDRLDLDAH VYETDPSFLT ASHDEAPVEE TLVTDLLKSN 190 200 210 220 230 240 CPVTGQPDWG SVQIHYVGAP IDHAGLLRYI ISFRNHTGFH EQCVERIFVD ILRACQPVKL 250 260 270 AVYARYTRRG GLDINPFRTN YNQPMPDNAR TARQ

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000010 Genomic DNA. Translation: AAU49002.1.
RefSeq	YP_102019.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3090187.
GenomeReviews	Gene locus BMA0180 in contig CP000010_GR.
KEGG	bma:BMA0180.
TIGR	BMA0180.
Phylogenomic databases
HOGENOM	Q62MP8.
OMA	Q62MP8. EHNEFHE.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_0180-MON.
BRENDA	1.7.1.13. 260531.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURMA

Accession

Primary (citable) accession number: Q62MP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)