Reviewed, UniProtKB/Swiss-Prot Q62LN5 (ASTD_BURMA)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	BMA0594

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262388
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62LN5-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: DB4B0E0D06184BA2	FASTA	487	51,869
	10 20 30 40 50 60 MTELFIDGAW RDGAGPVFAS RNPGTGEPVW EGAGASADDV ERAVASARRA FAAWSALDLD 70 80 90 100 110 120 ARCAIVKRFA ALLVERKEAL ATMIGRETGK PLWEARTEVA SMAAKVDVSI AAYHERTGER 130 140 150 160 170 180 RSPTADGVAV LRHRPHGVVA VFGPYNFPGH LPNGHIVPAL IAGNTVVFKP SELAPGVARA 190 200 210 220 230 240 TVEIWRDAGL PAGVLNLVQG EKDTGVALAN HRQIDGLFFT GSSDTGTLLH RQFGGRPEIV 250 260 270 280 290 300 LALEMGGNNP LVVADVEDID AAVHHAIQSA FLSAGQRCTC ARRILVPRGA FGDRFLERFA 310 320 330 340 350 360 DVASRITADV YDADPQPFMG AVISARAASR LVAAQAKLLE LGAAPIIEMR QRDPALGFVN 370 380 390 400 410 420 ASILDVTPVR ELPDEEHFGP LAQIVRYTDL DDAIARANDT AFGLSAGLLA DDETVWNTFR 430 440 450 460 470 480 RTIRAGIVNW NRPTNGASSA APFGGAGRSG NHRPSAYYAA DYCAYPMASV ESAQLQMPAN LSPGLHF

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000010 Genomic DNA. Translation: AAU49290.1.
RefSeq	YP_102384.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3088507.
GenomeReviews	Gene locus BMA0594 in contig CP000010_GR.
KEGG	bma:BMA0594.
TIGR	BMA0594.
Phylogenomic databases
HOGENOM	Q62LN5.
OMA	Q62LN5. EMTQPQA.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_0594-MON.
BRENDA	1.2.1.71. 260531.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_BURMA

Accession

Primary (citable) accession number: Q62LN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)