Q62K79 (LEXA_BURMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	BMA1211

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	LexA repressor HAMAP MF_00015		PRO_0000170021
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	133	1	For autocatalytic cleavage activity By similarity
	Active site	170	1	For autocatalytic cleavage activity By similarity
	Site	98 – 99	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62K79 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 713C9B66594842E5	FASTA	215	23,199
	10 20 30 40 50 60 MIKLTARQQQ VFDLIRRAIE RSGFPPTRAE IAAELGFSSP NAAEEHLRAL ARKGVIELAA 70 80 90 100 110 120 GASRGIRLLG IDDAPHQLTL PHAALMQLSL PLVGRVAAGS PILAQEHISQ HYACDPALFS 130 140 150 160 170 180 SKPDYLLKVR GLSMRDAGIL DGDLLAVQKR TEAKDGQIIV ARLGDDVTVK RLKRRPGGVE 190 200 210 LIAENPDYEN IFVKAGSAEF ALEGIAVGLI RPGEF

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000010 Genomic DNA. Translation: AAU47472.1.
RefSeq	YP_102890.1. NC_006348.1.
3D structure databases
ProteinModelPortal	Q62K79.
SMR	Q62K79. Positions 1-69, 90-215.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3089988.
GenomeReviews	Gene locus BMA1211 in contig CP000010_GR.
KEGG	bma:BMA1211.
PATRIC	19117440. VBIBurMal55007_1246.
TIGR	BMA1211.
Phylogenomic databases
HOGENOM	HBG679610.
OMA	IAENPDF.
PhylomeDB	Q62K79.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_1211-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01356.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BURMA

Accession

Primary (citable) accession number: Q62K79

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program