Q62JB3 (DAPD_BURMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
EC=2.3.1.117

Alternative name(s):
Tetrahydrodipicolinate N-succinyltransferase
Short name=THDP succinyltransferase
Short name=THP succinyltransferase
Short name=Tetrahydropicolinate succinylase

Gene names

Name:	dapD
Ordered Locus Names:	BMA1566

Organism

Burkholderia mallei (strain ATCC 23344) [Complete proteome] [HAMAP]

Taxonomic identifier

243160 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia mallei

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate. HAMAP-Rule MF_00811
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3. HAMAP-Rule MF_00811
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase HAMAP-Rule MF_00811		PRO_0000196926
Sites
	Binding site	106	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62JB3 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: CCC4692B397A0EF4	FASTA	275	29,508
	10 20 30 40 50 60 MSQQLQQIID NAWENRAELS PKAASAEIRE AVAHAIEQLD RGALRVAEKI DGAWTVHQWL 70 80 90 100 110 120 KKAVLLSFRL EDNAPMPAGG YSQFYDKVPS KFANYTAEDF AAGGFRVVPP AIARRGSFIA 130 140 150 160 170 180 KNVVLMPSYT NIGAYVDEGT MVDTWATVGS CAQIGKNVHL SGGVGIGGVL EPLQANPVII 190 200 210 220 230 240 EDNCFIGARS EVVEGVIVEE NSVISMGVYL GQSTKIYDRE TGEVTYGRIP AGSVVVAGNL 250 260 270 PAKDGTHSLY CAVIVKKVDA KTRAKVGLNE LLRGD

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000010 Genomic DNA. Translation: AAU47910.1.
RefSeq	YP_103206.1. NC_006348.1.
3D structure databases
ProteinModelPortal	Q62JB3.
SMR	Q62JB3. Positions 3-273.
ModBase	Search...
Protein-protein interaction databases
STRING	243160.BMA1566.
Proteomic databases
PRIDE	Q62JB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU47910; AAU47910; BMA1566.
GeneID	3089179.
KEGG	bma:BMA1566.
PATRIC	19118192. VBIBurMal55007_1613.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2171.
HOGENOM	HOG000003295.
KO	K00674.
OMA	NQWAKKA.
ProtClustDB	PRK11830.
Enzyme and pathway databases
UniPathway	UPA00034; UER00019.
Family and domain databases
Gene3D	1.10.166.10. 1 hit.
HAMAP	MF_00811. DapD.
InterPro	IPR005664. DapD_Trfase_Hexpep_rpt_fam. IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR023180. THP_succinylTrfase_dom1. IPR011004. Trimer_LpxA-like. [Graphical view]
PANTHER	PTHR19136:SF52. PTHR19136:SF52. 1 hit.
Pfam	PF00132. Hexapep. 2 hits. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR00965. dapD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPD_BURMA

Accession

Primary (citable) accession number: Q62JB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program