Q62HZ7 (NTPA_BURMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BMA2099

Organism

Burkholderia mallei (strain ATCC 23344) [Complete proteome] [HAMAP]

Taxonomic identifier

243160 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia mallei

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP-Rule MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP-Rule MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleoside triphosphate catabolic process Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Non-canonical purine NTP pyrophosphatase HAMAP-Rule MF_01405		PRO_0000178143
Regions
	Region	19 – 24	6	Substrate binding By similarity
	Region	80 – 81	2	Substrate binding By similarity
Sites
	Metal binding	51	1	Magnesium or manganese By similarity
	Metal binding	80	1	Magnesium or manganese By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62HZ7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 05B46B6B4E4EA876	FASTA	210	22,179
	10 20 30 40 50 60 MTMSHASPDA ARSRIVLASN NPGKLREFAA LFSTAGIDIV PQGELGVSEA DEPHATFVEN 70 80 90 100 110 120 ALAKARHASR ATGLPAVADD SGLCVPALLG APGVYSARYA QRAGREKSDA ANNAYLVEQL 130 140 150 160 170 180 REVADRRAYY YCVLALVRHA DDPEPLIAEG RWAGEIVDAP RGAHGFGYDP HFFVPALGAT 190 200 210 AAELDPAAKN AASHRALALK ALVARLGEIR

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000010 Genomic DNA. Translation: AAU50021.1.
RefSeq	YP_103673.1. NC_006348.1.
3D structure databases
ProteinModelPortal	Q62HZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	243160.BMA2099.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU50021; AAU50021; BMA2099.
GeneID	3088734.
KEGG	bma:BMA2099.
PATRIC	19119319. VBIBurMal55007_2167.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HOG000293319.
KO	K02428.
OMA	DGTLVWP.
ProtClustDB	PRK00120.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase.
InterPro	IPR002637. Ham1p-like. IPR020922. NTPase. [Graphical view]
PANTHER	PTHR11067. PTHR11067. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BURMA

Accession

Primary (citable) accession number: Q62HZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 25, 2004
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program