Q62HW8 (GLK_BURMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein glk

Including the following 2 domains:

1. Glucokinase
   EC=2.7.1.2
   Alternative name(s):
   Glucose kinase
2. Putative HTH-type transcriptional regulator

Gene names

Name:	glk
Ordered Locus Names:	BMA2132

Organism

Burkholderia mallei (strain ATCC 23344) [Complete proteome] [HAMAP]

Taxonomic identifier

243160 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › Burkholderia mallei

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP-Rule MF_00524
Subcellular location	Membrane; Single-pass membrane protein Potential HAMAP-Rule MF_00524.
Sequence similarities	In the N-terminal section; belongs to the bacterial glucokinase family. Contains 1 HTH rpiR-type DNA-binding domain. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Glycolysis Transcription Transcription regulation
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro glucokinase activity Inferred from electronic annotation. Source: EC sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Bifunctional protein glk HAMAP-Rule MF_00524		PRO_0000268797
Regions
	Transmembrane	576 – 596	21	Helical; Potential
	Domain	341 – 417	77	HTH rpiR-type
	Domain	461 – 600	140	SIS
	Nucleotide binding	23 – 28	6	ATP Potential
	DNA binding	377 – 396	20	H-T-H motif By similarity
	Region	1 – 340	340	Glucokinase HAMAP-Rule MF_00524
	Region	341 – 641	301	Putative HTH-type transcriptional regulator HAMAP-Rule MF_00524
	Compositional bias	591 – 639	49	Ala-rich HAMAP-Rule MF_00524

Sequences

	Sequence		Length	Mass (Da)
	Q62HW8 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 65D23DA89E0AD2EA	FASTA	641	68,376
	10 20 30 40 50 60 MSTGAQTKAA AASQHADGPR LLADVGGTNA RFALETGPGE ITQIRVYPGA EYPTLTDAIR 70 80 90 100 110 120 KYLKDAKIGR VNHAAIAIAN PVDGDQVRMT NHNWSFSIEA TRRALGFDTL LVVNDFTALA 130 140 150 160 170 180 MALPGLTDAQ RVQIGGGTRR QNSVIGLMGP GTGLGVSGLI PADDRWIALG SEGGHATFAP 190 200 210 220 230 240 MDEREDLVLQ YARRKYPHVS FERVCAGPGM EIIYRALAAR DKKRIAANVD TADIVERAHA 250 260 270 280 290 300 GDALALEAVE CFCAILGTFA GNLAVTLGAL GGIYIGGGVV PKLGELFMRS PFRARFEAKG 310 320 330 340 350 360 RFEAYLANIP TYLITAEYPA FLGVSAILAE QLSNRTGGAS SAVFERIRQM RDALTPAERR 370 380 390 400 410 420 VADLALNHPR SIINDPIVNI ARKADVSQPT VIRFCRSLGC QGLSDFKLKL ATGLTGTIPM 430 440 450 460 470 480 SHSQVHLGDT ATDFGAKVLD NTVSAILQLR EHLNFEHVEQ AIDILNNARR IEFYGLGNSN 490 500 510 520 530 540 IVAQDAHYKF FRFGIPTIAY GDLYMQAASA ALLGKGDVIV AVSKSGRAPE LLRVLDVAMQ 550 560 570 580 590 600 AGAKVIAITS SNTPLAKRAT VALETDHIEM RESQLSMISR ILHLVMIDIL AVGVAIRRAA 610 620 630 640 PNAELAEAMA RAKARAGASA GDEAADVLDW LSHGAAPAAK D

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000010 Genomic DNA. Translation: AAU49971.1.
RefSeq	YP_103702.1. NC_006348.1.
3D structure databases
ProteinModelPortal	Q62HW8.
ModBase	Search...
Protein-protein interaction databases
STRING	243160.BMA2132.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU49971; AAU49971; BMA2132.
GeneID	3091251.
KEGG	bma:BMA2132.
PATRIC	19119389. VBIBurMal55007_2200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1737.
HOGENOM	HOG000257466.
KO	K00845.
OMA	CGILGTF.
ProtClustDB	PRK14101.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00524. Glucokinase. Fused.
InterPro	IPR003836. Glucokinase. IPR000281. HTH_RpiR. IPR001347. SIS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF02685. Glucokinase. 1 hit. PF01418. HTH_6. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00749. glk. 1 hit.
PROSITE	PS51071. HTH_RPIR. 1 hit. PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLK_BURMA

Accession

Primary (citable) accession number: Q62HW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2004
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program