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Q62HT7 (TRMB_BURMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BMA2167
OrganismBurkholderia mallei (strain ATCC 23344) [Complete proteome] [HAMAP]
Taxonomic identifier243160 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171310

Regions

Region240 – 2434Substrate binding By similarity

Sites

Active site1691 By similarity
Binding site941S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1461S-adenosyl-L-methionine By similarity
Binding site1691S-adenosyl-L-methionine By similarity
Binding site1731Substrate By similarity
Binding site2051Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q62HT7 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: ABA972FFA4374B2F

FASTA26428,986
        10         20         30         40         50         60 
MIHDDDPNAP GAPHDDATAA PASATRAAPA AGDDDDANPL HLRRIRSFVT RAGRVSTGQR 

        70         80         90        100        110        120 
RAIDELGPRF VIPYGSAQPD WDAIFGRRAP RVLEIGFGMG ASTAEIAALR PGDDFIGVEV 

       130        140        150        160        170        180 
HEPGVGALLK LIGEQQLSNI RIIQHDAVEV LAQMIAPDSL DGVHIFFPDP WHKARHHKRR 

       190        200        210        220        230        240 
LIQPPFVAQL AAHLKPGAYL HCATDWQNYA EQMLEVLSAD PSLENTAQNY APRPGYRPVT 

       250        260 
KFERRGLRLG HGVWDLVFRK KHAG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000010 Genomic DNA. Translation: AAU49915.1.
RefSeqYP_103733.1. NC_006348.1.

3D structure databases

ProteinModelPortalQ62HT7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243160.BMA2167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU49915; AAU49915; BMA2167.
GeneID3091157.
KEGGbma:BMA2167.
PATRIC19119459. VBIBurMal55007_2234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BURMA
AccessionPrimary (citable) accession number: Q62HT7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 25, 2004
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways