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Q62HL4

- SYI1_BURMA

UniProt

Q62HL4 - SYI1_BURMA

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Protein
Isoleucine--tRNA ligase 1
Gene
ileS1, ileS-1, BMA2242
Organism
Burkholderia mallei (strain ATCC 23344)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylate By similarity
Binding sitei625 – 6251ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 1 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 1
Short name:
IleRS 1
Gene namesi
Name:ileS1
Synonyms:ileS-1
Ordered Locus Names:BMA2242
OrganismiBurkholderia mallei (strain ATCC 23344)
Taxonomic identifieri243160 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000006693: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligase 1UniRule annotation
PRO_0000098368Add
BLAST

Proteomic databases

PRIDEiQ62HL4.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243160.BMA2242.

Structurei

3D structure databases

ProteinModelPortaliQ62HL4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotation
Add
BLAST
Motifi622 – 6265"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62HL4-1 [UniParc]FASTAAdd to Basket

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MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVEDW EARGVYEKIR    50
AASQGRPKFI LHDGPPYANG DIHLGHAVNK ILKDMVVKSR NMAGFDAPYV 100
PGWDCHGMPI EIQIEKRFGK SLPAAEVMAK ARAYATEQIE KQKVGFKRLG 150
VLGEWGNPYK TMNFQNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL 200
AEAEVEYKDR TDPTIDVLFA FAEPEKTAHA FGLAELPRAE GGIVIWTTTP 250
WTIPANQALN LHPEIVYALV DTERGLLVMA EERVEACMKD FGLTGRVIAR 300
TPGEKLANLR FHHPLAAAHP GYKRTSPVYL GDYVTTDTGT GVVHSSPAYG 350
VEDFTSCKAH GMTDSDIINP VMGDGRYIES LPLFGGLTIW DANPKIVDAL 400
KAAGSLLRNE RYAHSYMHCW RHKTPIIYRA TSQWFAGMDT QPAGGGKTLR 450
ETALDAVDAT AFYPSWGKQR LHAMIANRPD WTLSRQRQWG VPMAFFVHKE 500
TGELHPRTLE LLEEVAKRVE RQGIEAWQTL DARELIGDDA NLYEKNRDTL 550
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML 600
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA 650
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAKDALPAGQ 700
WLEIDRYAVA FAAQLQAELL AHYEKYEFHP VVAKLQTFCS EDLGGFYLDV 750
LKDRLYTSAP ASPARRSAQT ALYHVTQGLL RVLAPFLSFT AEEAWRVFQP 800
QSDTIFTETY YAYPEIAGAE ALIAKWTLLR DVRGDVTKAL EEARTANRIG 850
SSLQAQVEVR ASGARYDALA SLGDDLKFVL ITSAATVVKV DAQGDESVDV 900
AASTYPKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE TRSAA 945
Length:945
Mass (Da):105,680
Last modified:October 25, 2004 - v1
Checksum:i5E7C07BF9C19C03C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000010 Genomic DNA. Translation: AAU50245.1.
RefSeqiYP_103806.1. NC_006348.1.

Genome annotation databases

EnsemblBacteriaiAAU50245; AAU50245; BMA2242.
GeneIDi3090843.
KEGGibma:BMA2242.
PATRICi19119605. VBIBurMal55007_2307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000010 Genomic DNA. Translation: AAU50245.1 .
RefSeqi YP_103806.1. NC_006348.1.

3D structure databases

ProteinModelPortali Q62HL4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243160.BMA2242.

Proteomic databases

PRIDEi Q62HL4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU50245 ; AAU50245 ; BMA2242 .
GeneIDi 3090843.
KEGGi bma:BMA2242.
PATRICi 19119605. VBIBurMal55007_2307.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23344.

Entry informationi

Entry nameiSYI1_BURMA
AccessioniPrimary (citable) accession number: Q62HL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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