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Protein

Thiazole synthase

Gene

thiG

Organism
Burkholderia mallei (strain ATCC 23344)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Schiff-base intermediate with DXPUniRule annotation
Binding sitei165 – 1651DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:BMA2728
OrganismiBurkholderia mallei (strain ATCC 23344)
Taxonomic identifieri243160 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006693 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Thiazole synthasePRO_0000162799Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi243160.BMA2728.

Structurei

3D structure databases

ProteinModelPortaliQ62GC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1932DXP bindingUniRule annotation
Regioni214 – 2152DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62GC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPLSSADTL TLHGQTFASR VLLGTSRYPS LQSLSDSIAA ARPGMVTVAL
60 70 80 90 100
RRQMNAGAAE AGFFELLKRH DVPLLPNTAG CQTIAEAVTT AQMAREVFET
110 120 130 140 150
DWIKLELIGD DYTLQPDPVG LIEAATLLVK DGFKVLPYCT EDLVIARRLL
160 170 180 190 200
DAGCEALMPW GAPIGTGKGI VNPYGLRVLR ERLPDVPLIV DAGLGVPSHA
210 220 230 240 250
CQVMEWGFDG VLLNTAVSQA THPEIMARAF ARGVEAGRAA YLAGPMDARE
260 270
SAHASTPVVG MPFWHQDGSH A
Length:271
Mass (Da):28,942
Last modified:October 25, 2004 - v1
Checksum:iAEE947181AE99414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000010 Genomic DNA. Translation: AAU48295.1.
RefSeqiWP_004199935.1. NC_006348.1.
YP_104248.1. NC_006348.1.

Genome annotation databases

EnsemblBacteriaiAAU48295; AAU48295; BMA2728.
GeneIDi3091410.
KEGGibma:BMA2728.
PATRICi19120607. VBIBurMal55007_2797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000010 Genomic DNA. Translation: AAU48295.1.
RefSeqiWP_004199935.1. NC_006348.1.
YP_104248.1. NC_006348.1.

3D structure databases

ProteinModelPortaliQ62GC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243160.BMA2728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU48295; AAU48295; BMA2728.
GeneIDi3091410.
KEGGibma:BMA2728.
PATRICi19120607. VBIBurMal55007_2797.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23344.

Entry informationi

Entry nameiTHIG_BURMA
AccessioniPrimary (citable) accession number: Q62GC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 25, 2004
Last modified: November 11, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.