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Protein

Thiazole synthase

Gene

thiG

Organism
Burkholderia mallei (strain ATCC 23344)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Schiff-base intermediate with DXPUniRule annotation1
Binding sitei165DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciBMAL243160:G1G3W-8588-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:BMA2728
OrganismiBurkholderia mallei (strain ATCC 23344)
Taxonomic identifieri243160 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006693 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627991 – 271Thiazole synthaseAdd BLAST271

Proteomic databases

PRIDEiQ62GC6

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi243160.BMA2728

Structurei

3D structure databases

ProteinModelPortaliQ62GC6
SMRiQ62GC6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 193DXP bindingUniRule annotation2
Regioni214 – 215DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q62GC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPLSSADTL TLHGQTFASR VLLGTSRYPS LQSLSDSIAA ARPGMVTVAL
60 70 80 90 100
RRQMNAGAAE AGFFELLKRH DVPLLPNTAG CQTIAEAVTT AQMAREVFET
110 120 130 140 150
DWIKLELIGD DYTLQPDPVG LIEAATLLVK DGFKVLPYCT EDLVIARRLL
160 170 180 190 200
DAGCEALMPW GAPIGTGKGI VNPYGLRVLR ERLPDVPLIV DAGLGVPSHA
210 220 230 240 250
CQVMEWGFDG VLLNTAVSQA THPEIMARAF ARGVEAGRAA YLAGPMDARE
260 270
SAHASTPVVG MPFWHQDGSH A
Length:271
Mass (Da):28,942
Last modified:October 25, 2004 - v1
Checksum:iAEE947181AE99414
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000010 Genomic DNA Translation: AAU48295.1
RefSeqiWP_004199935.1, NC_006348.1
YP_104248.1, NC_006348.1

Genome annotation databases

EnsemblBacteriaiAAU48295; AAU48295; BMA2728
GeneIDi3091410
KEGGibma:BMA2728
PATRICifig|243160.12.peg.2797

Similar proteinsi

Entry informationi

Entry nameiTHIG_BURMA
AccessioniPrimary (citable) accession number: Q62GC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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