Reviewed, UniProtKB/Swiss-Prot Q62CU8 (NADE_BURMA)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BMAA0760

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099008
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62CU8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 8AE2C18E0B0BCEAF	FASTA	284	30,863
	10 20 30 40 50 60 MSRPDQAARR RAIAAELHVS PTFDARDEAE RRIGFVADYL RTAGLRACVL GISGGIDSST 70 80 90 100 110 120 AGRLAQLAVE RLRASGYDAR FVAMRLPYGA QHDEADARRA LAFVRADETL TVDVKPAADA 130 140 150 160 170 180 MLAALAAGGL AYLDHAQQDF VLGNIKARER MIAQYAVAGA RNGVVIGTDH AAESVMGFFT 190 200 210 220 230 240 KFGDGGADVL PLAGLTKRRV RALARMLGAD EPLVLKTPTA DLETLRPQRP DEHAYGITYE 250 260 270 280 QIDDFLEGKP MDDAVAETVL RFYDATHHKR ALPYTMFDWP GHPA

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000011 Genomic DNA. Translation: AAU46930.1.
RefSeq	YP_105480.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3086366.
GenomeReviews	Gene locus BMAA0760 in contig CP000011_GR.
KEGG	bma:BMAA0760.
TIGR	BMAA0760.
Phylogenomic databases
HOGENOM	Q62CU8.
OMA	ADLEEDR.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_A0760-MON.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURMA

Accession

Primary (citable) accession number: Q62CU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)